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P25815 (S100P_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein S100-P
Alternative name(s):
Migration-inducing gene 9 protein
Short name=MIG9
Protein S100-E
S100 calcium-binding protein P
Gene names
Name:S100P
Synonyms:S100E
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length95 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE). Ref.10 Ref.12 Ref.14

Subunit structure

Homodimer and heterodimer with S100A1. Interacts with S100PBP and S100Z. Interacts with CACYBP in a calcium-dependent manner. Dimeric form binds to and activates EZR/Ezrin by unmasking its F-actin binding sites. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity. Ref.7 Ref.9 Ref.11 Ref.12 Ref.14

Subcellular location

Nucleus. Cytoplasm. Cell projectionmicrovillus membrane. Note: Colocalizes with S100PBP in the nucleus. Colocolizes with EZR in the microvilli in a calcium-dependent manner. Ref.11 Ref.12

Tissue specificity

Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias. Ref.8 Ref.11

Miscellaneous

This protein binds two calcium ions.

Sequence similarities

Belongs to the S-100 family.

Contains 2 EF-hand domains.

Sequence caution

The sequence AAS00487.1 differs from that shown. Reason: Sequencing error in Met-1 codon.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AGERQ151092EBI-743700,EBI-1646426
CacybpQ9CXW32EBI-743700,EBI-767146From a different organism.
S100A1P232977EBI-743700,EBI-743686

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9595Protein S100-P
PRO_0000144032

Regions

Domain12 – 4736EF-hand 1
Domain49 – 8436EF-hand 2
Calcium binding19 – 32141; low affinity
Calcium binding62 – 73122; high affinity

Experimental info

Mutagenesis21 – 233Missing in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with C-64, K-68 and S-73. Ref.12 Ref.14
Mutagenesis641N → C in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with 21-S--G-23, K-68 and S-73. Ref.12 Ref.14
Mutagenesis681Q → K in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with 21-S--G-23, C-64 and S-73. Ref.12 Ref.14
Mutagenesis731E → S in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with 21-S--G-23, C-64 and K-68. Ref.12 Ref.14
Sequence conflict121I → M in AAS00487. Ref.4
Sequence conflict321E → T AA sequence Ref.6
Sequence conflict441F → E AA sequence Ref.6

Secondary structure

.............. 95
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25815 [UniParc].

Last modified December 1, 1992. Version 2.
Checksum: 786E6E3F3EACC6C1

FASTA9510,400
        10         20         30         40         50         60 
MTELETAMGM IIDVFSRYSG SEGSTQTLTK GELKVLMEKE LPGFLQSGKD KDAVDKLLKD 

        70         80         90 
LDANGDAQVD FSEFIVFVAA ITSACHKYFE KAGLK 

« Hide

References

« Hide 'large scale' references
[1]"S100P, a novel Ca(2+)-binding protein from human placenta. cDNA cloning, recombinant protein expression and Ca2+ binding properties."
Becker T., Gerke V., Kube E., Weber K.
Eur. J. Biochem. 207:541-547(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"Cloning, expression and characterization of a novel human calcium-binding S100 gene."
Jin G., Wang S., Chen J.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Identification of a human migration-inducing gene 9 (MIG9)."
Kim J.W.
Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[6]"Purification and characterization of a new member of the S-100 protein family from human placenta."
Emoto Y., Kobayashi R., Akatsuka H., Hidaka H.
Biochem. Biophys. Res. Commun. 182:1246-1253(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-91.
Tissue: Placenta.
[7]"Ca2+-dependent binding and activation of dormant ezrin by dimeric S100P."
Koltzscher M., Neumann C., Konig S., Gerke V.
Mol. Biol. Cell 14:2372-2384(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EZR.
[8]"Characterization of the tissue-specific expression of the s100P gene which encodes an EF-hand Ca2+-binding protein."
Jin G., Wang S., Hu X., Jing Z., Chen J., Ying K., Xie Y., Mao Y.
Mol. Biol. Rep. 30:243-248(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"Heterodimeric interaction and interfaces of S100A1 and S100P."
Wang G., Zhang S., Fernig D.G., Spiller D., Martin-Fernandez M., Zhang H., Ding Y., Rao Z., Rudland P.S., Barraclough R.
Biochem. J. 382:375-383(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, INTERACTION WITH S100A1.
[10]"S100P stimulates cell proliferation and survival via receptor for activated glycation end products (RAGE)."
Arumugam T., Simeone D.M., Schmidt A.M., Logsdon C.D.
J. Biol. Chem. 279:5059-5065(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Expression of S100P and its novel binding partner S100PBPR in early pancreatic cancer."
Dowen S.E., Crnogorac-Jurcevic T., Gangeswaran R., Hansen M., Eloranta J.J., Bhakta V., Brentnall T.A., Luettges J., Kloeppel G., Lemoine N.R.
Am. J. Pathol. 166:81-92(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH S100PBP, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[12]"Generation and characterization of a novel, permanently active S100P mutant."
Austermann J., Nazmi A.R., Heil A., Fritz G., Kolinski M., Filipek S., Gerke V.
Biochim. Biophys. Acta 1793:1078-1085(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH EZR, SUBCELLULAR LOCATION, MUTAGENESIS OF 21-SER--GLY-23; ASN-64; GLN-68 AND GLU-73.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation."
Yamaguchi F., Umeda Y., Shimamoto S., Tsuchiya M., Tokumitsu H., Tokuda M., Kobayashi R.
J. Biol. Chem. 287:13787-13798(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PPP5C, MUTAGENESIS OF 21-SER--GLY-23; ASN-64; GLN-68 AND GLU-73.
[15]"The crystal structure at 2A resolution of the Ca2+ -binding protein S100P."
Zhang H., Wang G., Ding Y., Wang Z., Barraclough R., Rudland P.S., Fernig D.G., Rao Z.
J. Mol. Biol. 325:785-794(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[16]"NMR structure of the Apo-S100P protein."
Lee Y.C., Volk D.E., Thiviyanathan V., Kleerekoper Q., Gribenko A.V., Zhang S., Gorenstein D.G., Makhatadze G.I., Luxon B.A.
J. Biomol. NMR 29:399-402(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X65614 mRNA. Translation: CAA46566.1.
AF539739 mRNA. Translation: AAO41114.1.
BT007289 mRNA. Translation: AAP35953.1.
AY423724 mRNA. Translation: AAS00487.1. Sequence problems.
BC006819 mRNA. Translation: AAH06819.1.
CCDSCCDS3391.1.
PIRS24146.
RefSeqNP_005971.1. NM_005980.2.
UniGeneHs.2962.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1J55X-ray2.00A1-95[»]
1OZONMR-A/B1-95[»]
ProteinModelPortalP25815.
SMRP25815. Positions 1-95.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112194. 15 interactions.
IntActP25815. 5 interactions.
MINTMINT-239013.
STRING9606.ENSP00000296370.

Chemistry

DrugBankDB01003. Cromoglicate.

PTM databases

PhosphoSiteP25815.

Polymorphism databases

DMDM134142.

Proteomic databases

MaxQBP25815.
PaxDbP25815.
PeptideAtlasP25815.
PRIDEP25815.

Protocols and materials databases

DNASU6286.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000296370; ENSP00000296370; ENSG00000163993.
GeneID6286.
KEGGhsa:6286.
UCSCuc003gjl.3. human.

Organism-specific databases

CTD6286.
GeneCardsGC04P006698.
HGNCHGNC:10504. S100P.
HPACAB012900.
HPA019502.
MIM600614. gene.
neXtProtNX_P25815.
PharmGKBPA34913.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG78209.
HOGENOMHOG000246968.
HOVERGENHBG001479.
InParanoidP25815.
OMALTSACHK.
OrthoDBEOG7R833W.
PhylomeDBP25815.
TreeFamTF332727.

Gene expression databases

BgeeP25815.
CleanExHS_S100P.
GenevestigatorP25815.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
IPR028494. S100P.
[Graphical view]
PANTHERPTHR11639:SF69. PTHR11639:SF69. 1 hit.
PfamPF01023. S_100. 1 hit.
[Graphical view]
PROSITEPS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP25815.
GeneWikiS100P.
GenomeRNAi6286.
NextBio24409.
PROP25815.
SOURCESearch...

Entry information

Entry nameS100P_HUMAN
AccessionPrimary (citable) accession number: P25815
Secondary accession number(s): Q5J7W2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM