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Protein

Protein S100-P

Gene

S100P

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi19 – 32141; low affinityAdd
BLAST
Calcium bindingi62 – 73122; high affinityAdd
BLAST

GO - Molecular functioni

  1. calcium-dependent protein binding Source: HGNC
  2. calcium ion binding Source: ProtInc
  3. magnesium ion binding Source: HGNC

GO - Biological processi

  1. endothelial cell migration Source: UniProtKB
  2. response to organic substance Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein S100-P
Alternative name(s):
Migration-inducing gene 9 protein
Short name:
MIG9
Protein S100-E
S100 calcium-binding protein P
Gene namesi
Name:S100P
Synonyms:S100E
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:10504. S100P.

Subcellular locationi

Nucleus. Cytoplasm. Cell projectionmicrovillus membrane
Note: Colocalizes with S100PBP in the nucleus. Colocolizes with EZR in the microvilli in a calcium-dependent manner.

GO - Cellular componenti

  1. cytoplasm Source: HGNC
  2. extracellular vesicular exosome Source: UniProtKB
  3. microvillus membrane Source: UniProtKB-SubCell
  4. nucleus Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi21 – 233Missing in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with C-64, K-68 and S-73. 2 Publications
Mutagenesisi64 – 641N → C in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with 21-S--G-23, K-68 and S-73. 2 Publications
Mutagenesisi68 – 681Q → K in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with 21-S--G-23, C-64 and S-73. 2 Publications
Mutagenesisi73 – 731E → S in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with 21-S--G-23, C-64 and K-68. 2 Publications

Organism-specific databases

PharmGKBiPA34913.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9595Protein S100-PPRO_0000144032Add
BLAST

Proteomic databases

MaxQBiP25815.
PaxDbiP25815.
PeptideAtlasiP25815.
PRIDEiP25815.

PTM databases

PhosphoSiteiP25815.

Expressioni

Tissue specificityi

Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias.2 Publications

Gene expression databases

BgeeiP25815.
CleanExiHS_S100P.
GenevestigatoriP25815.

Organism-specific databases

HPAiCAB012900.
HPA019502.

Interactioni

Subunit structurei

Homodimer and heterodimer with S100A1. Interacts with S100PBP and S100Z. Interacts with CACYBP in a calcium-dependent manner. Dimeric form binds to and activates EZR/Ezrin by unmasking its F-actin binding sites. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AGERQ151092EBI-743700,EBI-1646426
CacybpQ9CXW32EBI-743700,EBI-767146From a different organism.
S100A1P232977EBI-743700,EBI-743686

Protein-protein interaction databases

BioGridi112194. 18 interactions.
IntActiP25815. 5 interactions.
MINTiMINT-239013.
STRINGi9606.ENSP00000296370.

Structurei

Secondary structure

1
95
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1816Combined sources
Beta strandi21 – 233Combined sources
Beta strandi25 – 284Combined sources
Helixi30 – 4011Combined sources
Helixi44 – 463Combined sources
Helixi53 – 619Combined sources
Beta strandi63 – 708Combined sources
Helixi71 – 9222Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J55X-ray2.00A1-95[»]
1OZONMR-A/B1-95[»]
2MJWNMR-B/D1-94[»]
ProteinModelPortaliP25815.
SMRiP25815. Positions 1-95.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25815.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 4736EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini49 – 8436EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the S-100 family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG78209.
GeneTreeiENSGT00760000119034.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP25815.
OMAiNGDSEVD.
OrthoDBiEOG7R833W.
PhylomeDBiP25815.
TreeFamiTF332727.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
IPR028494. S100P.
[Graphical view]
PANTHERiPTHR11639:SF69. PTHR11639:SF69. 1 hit.
PfamiPF01023. S_100. 1 hit.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25815-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTELETAMGM IIDVFSRYSG SEGSTQTLTK GELKVLMEKE LPGFLQSGKD
60 70 80 90
KDAVDKLLKD LDANGDAQVD FSEFIVFVAA ITSACHKYFE KAGLK
Length:95
Mass (Da):10,400
Last modified:December 1, 1992 - v2
Checksum:i786E6E3F3EACC6C1
GO

Sequence cautioni

The sequence AAS00487.1 differs from that shown.Sequencing error in Met-1 codon.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121I → M in AAS00487. 1 PublicationCurated
Sequence conflicti32 – 321E → T AA sequence (PubMed:1540168)Curated
Sequence conflicti44 – 441F → E AA sequence (PubMed:1540168)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65614 mRNA. Translation: CAA46566.1.
AF539739 mRNA. Translation: AAO41114.1.
BT007289 mRNA. Translation: AAP35953.1.
AY423724 mRNA. Translation: AAS00487.1. Sequence problems.
BC006819 mRNA. Translation: AAH06819.1.
CCDSiCCDS3391.1.
PIRiS24146.
RefSeqiNP_005971.1. NM_005980.2.
UniGeneiHs.2962.

Genome annotation databases

EnsembliENST00000296370; ENSP00000296370; ENSG00000163993.
GeneIDi6286.
KEGGihsa:6286.
UCSCiuc003gjl.3. human.

Polymorphism databases

DMDMi134142.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65614 mRNA. Translation: CAA46566.1.
AF539739 mRNA. Translation: AAO41114.1.
BT007289 mRNA. Translation: AAP35953.1.
AY423724 mRNA. Translation: AAS00487.1. Sequence problems.
BC006819 mRNA. Translation: AAH06819.1.
CCDSiCCDS3391.1.
PIRiS24146.
RefSeqiNP_005971.1. NM_005980.2.
UniGeneiHs.2962.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J55X-ray2.00A1-95[»]
1OZONMR-A/B1-95[»]
2MJWNMR-B/D1-94[»]
ProteinModelPortaliP25815.
SMRiP25815. Positions 1-95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112194. 18 interactions.
IntActiP25815. 5 interactions.
MINTiMINT-239013.
STRINGi9606.ENSP00000296370.

Chemistry

DrugBankiDB01003. Cromoglicic acid.

PTM databases

PhosphoSiteiP25815.

Polymorphism databases

DMDMi134142.

Proteomic databases

MaxQBiP25815.
PaxDbiP25815.
PeptideAtlasiP25815.
PRIDEiP25815.

Protocols and materials databases

DNASUi6286.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296370; ENSP00000296370; ENSG00000163993.
GeneIDi6286.
KEGGihsa:6286.
UCSCiuc003gjl.3. human.

Organism-specific databases

CTDi6286.
GeneCardsiGC04P006700.
HGNCiHGNC:10504. S100P.
HPAiCAB012900.
HPA019502.
MIMi600614. gene.
neXtProtiNX_P25815.
PharmGKBiPA34913.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG78209.
GeneTreeiENSGT00760000119034.
HOGENOMiHOG000246968.
HOVERGENiHBG001479.
InParanoidiP25815.
OMAiNGDSEVD.
OrthoDBiEOG7R833W.
PhylomeDBiP25815.
TreeFamiTF332727.

Miscellaneous databases

EvolutionaryTraceiP25815.
GeneWikiiS100P.
GenomeRNAii6286.
NextBioi24409.
PROiP25815.
SOURCEiSearch...

Gene expression databases

BgeeiP25815.
CleanExiHS_S100P.
GenevestigatoriP25815.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
IPR028494. S100P.
[Graphical view]
PANTHERiPTHR11639:SF69. PTHR11639:SF69. 1 hit.
PfamiPF01023. S_100. 1 hit.
[Graphical view]
PROSITEiPS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "S100P, a novel Ca(2+)-binding protein from human placenta. cDNA cloning, recombinant protein expression and Ca2+ binding properties."
    Becker T., Gerke V., Kube E., Weber K.
    Eur. J. Biochem. 207:541-547(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  2. "Cloning, expression and characterization of a novel human calcium-binding S100 gene."
    Jin G., Wang S., Chen J.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Identification of a human migration-inducing gene 9 (MIG9)."
    Kim J.W.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  6. "Purification and characterization of a new member of the S-100 protein family from human placenta."
    Emoto Y., Kobayashi R., Akatsuka H., Hidaka H.
    Biochem. Biophys. Res. Commun. 182:1246-1253(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-91.
    Tissue: Placenta.
  7. "Ca2+-dependent binding and activation of dormant ezrin by dimeric S100P."
    Koltzscher M., Neumann C., Konig S., Gerke V.
    Mol. Biol. Cell 14:2372-2384(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EZR.
  8. "Characterization of the tissue-specific expression of the s100P gene which encodes an EF-hand Ca2+-binding protein."
    Jin G., Wang S., Hu X., Jing Z., Chen J., Ying K., Xie Y., Mao Y.
    Mol. Biol. Rep. 30:243-248(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  9. Cited for: SUBUNIT, INTERACTION WITH S100A1.
  10. "S100P stimulates cell proliferation and survival via receptor for activated glycation end products (RAGE)."
    Arumugam T., Simeone D.M., Schmidt A.M., Logsdon C.D.
    J. Biol. Chem. 279:5059-5065(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: INTERACTION WITH S100PBP, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  12. "Generation and characterization of a novel, permanently active S100P mutant."
    Austermann J., Nazmi A.R., Heil A., Fritz G., Kolinski M., Filipek S., Gerke V.
    Biochim. Biophys. Acta 1793:1078-1085(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EZR, SUBCELLULAR LOCATION, MUTAGENESIS OF 21-SER--GLY-23; ASN-64; GLN-68 AND GLU-73.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation."
    Yamaguchi F., Umeda Y., Shimamoto S., Tsuchiya M., Tokumitsu H., Tokuda M., Kobayashi R.
    J. Biol. Chem. 287:13787-13798(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPP5C, MUTAGENESIS OF 21-SER--GLY-23; ASN-64; GLN-68 AND GLU-73.
  15. "The crystal structure at 2A resolution of the Ca2+ -binding protein S100P."
    Zhang H., Wang G., Ding Y., Wang Z., Barraclough R., Rudland P.S., Fernig D.G., Rao Z.
    J. Mol. Biol. 325:785-794(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  16. Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiS100P_HUMAN
AccessioniPrimary (citable) accession number: P25815
Secondary accession number(s): Q5J7W2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: December 1, 1992
Last modified: February 4, 2015
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This protein binds two calcium ions.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.