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P25815

- S100P_HUMAN

UniProt

P25815 - S100P_HUMAN

Protein

Protein S100-P

Gene

S100P

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi19 – 32141; low affinityAdd
    BLAST
    Calcium bindingi62 – 73122; high affinityAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent protein binding Source: HGNC
    2. calcium ion binding Source: ProtInc
    3. magnesium ion binding Source: HGNC
    4. protein binding Source: IntAct

    GO - Biological processi

    1. endothelial cell migration Source: UniProtKB
    2. response to organic substance Source: UniProtKB

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein S100-P
    Alternative name(s):
    Migration-inducing gene 9 protein
    Short name:
    MIG9
    Protein S100-E
    S100 calcium-binding protein P
    Gene namesi
    Name:S100P
    Synonyms:S100E
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:10504. S100P.

    Subcellular locationi

    Nucleus. Cytoplasm. Cell projectionmicrovillus membrane
    Note: Colocalizes with S100PBP in the nucleus. Colocolizes with EZR in the microvilli in a calcium-dependent manner.

    GO - Cellular componenti

    1. cytoplasm Source: HGNC
    2. extracellular vesicular exosome Source: UniProt
    3. microvillus membrane Source: UniProtKB-SubCell
    4. nucleus Source: HGNC

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi21 – 233Missing in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with C-64, K-68 and S-73.
    Mutagenesisi64 – 641N → C in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with 21-S--G-23, K-68 and S-73. 2 Publications
    Mutagenesisi68 – 681Q → K in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with 21-S--G-23, C-64 and S-73. 2 Publications
    Mutagenesisi73 – 731E → S in S100Ppa; permanently active, interacts with EZR and PPP5C in absence of calcium; when associated with 21-S--G-23, C-64 and K-68. 2 Publications

    Organism-specific databases

    PharmGKBiPA34913.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9595Protein S100-PPRO_0000144032Add
    BLAST

    Proteomic databases

    MaxQBiP25815.
    PaxDbiP25815.
    PeptideAtlasiP25815.
    PRIDEiP25815.

    PTM databases

    PhosphoSiteiP25815.

    Expressioni

    Tissue specificityi

    Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias.2 Publications

    Gene expression databases

    BgeeiP25815.
    CleanExiHS_S100P.
    GenevestigatoriP25815.

    Organism-specific databases

    HPAiCAB012900.
    HPA019502.

    Interactioni

    Subunit structurei

    Homodimer and heterodimer with S100A1. Interacts with S100PBP and S100Z. Interacts with CACYBP in a calcium-dependent manner. Dimeric form binds to and activates EZR/Ezrin by unmasking its F-actin binding sites. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AGERQ151092EBI-743700,EBI-1646426
    CacybpQ9CXW32EBI-743700,EBI-767146From a different organism.
    S100A1P232977EBI-743700,EBI-743686

    Protein-protein interaction databases

    BioGridi112194. 15 interactions.
    IntActiP25815. 5 interactions.
    MINTiMINT-239013.
    STRINGi9606.ENSP00000296370.

    Structurei

    Secondary structure

    1
    95
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1816
    Beta strandi21 – 233
    Helixi30 – 4011
    Helixi44 – 463
    Helixi53 – 619
    Beta strandi63 – 708
    Helixi71 – 9222

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J55X-ray2.00A1-95[»]
    1OZONMR-A/B1-95[»]
    ProteinModelPortaliP25815.
    SMRiP25815. Positions 1-95.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP25815.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 4736EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini49 – 8436EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the S-100 family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG78209.
    HOGENOMiHOG000246968.
    HOVERGENiHBG001479.
    InParanoidiP25815.
    OMAiLTSACHK.
    OrthoDBiEOG7R833W.
    PhylomeDBiP25815.
    TreeFamiTF332727.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    IPR028494. S100P.
    [Graphical view]
    PANTHERiPTHR11639:SF69. PTHR11639:SF69. 1 hit.
    PfamiPF01023. S_100. 1 hit.
    [Graphical view]
    PROSITEiPS50222. EF_HAND_2. 1 hit.
    PS00303. S100_CABP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25815-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTELETAMGM IIDVFSRYSG SEGSTQTLTK GELKVLMEKE LPGFLQSGKD   50
    KDAVDKLLKD LDANGDAQVD FSEFIVFVAA ITSACHKYFE KAGLK 95
    Length:95
    Mass (Da):10,400
    Last modified:December 1, 1992 - v2
    Checksum:i786E6E3F3EACC6C1
    GO

    Sequence cautioni

    The sequence AAS00487.1 differs from that shown. Reason: Sequencing error in Met-1 codon.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121I → M in AAS00487. 1 PublicationCurated
    Sequence conflicti32 – 321E → T AA sequence (PubMed:1540168)Curated
    Sequence conflicti44 – 441F → E AA sequence (PubMed:1540168)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65614 mRNA. Translation: CAA46566.1.
    AF539739 mRNA. Translation: AAO41114.1.
    BT007289 mRNA. Translation: AAP35953.1.
    AY423724 mRNA. Translation: AAS00487.1. Sequence problems.
    BC006819 mRNA. Translation: AAH06819.1.
    CCDSiCCDS3391.1.
    PIRiS24146.
    RefSeqiNP_005971.1. NM_005980.2.
    UniGeneiHs.2962.

    Genome annotation databases

    EnsembliENST00000296370; ENSP00000296370; ENSG00000163993.
    GeneIDi6286.
    KEGGihsa:6286.
    UCSCiuc003gjl.3. human.

    Polymorphism databases

    DMDMi134142.

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65614 mRNA. Translation: CAA46566.1 .
    AF539739 mRNA. Translation: AAO41114.1 .
    BT007289 mRNA. Translation: AAP35953.1 .
    AY423724 mRNA. Translation: AAS00487.1 . Sequence problems.
    BC006819 mRNA. Translation: AAH06819.1 .
    CCDSi CCDS3391.1.
    PIRi S24146.
    RefSeqi NP_005971.1. NM_005980.2.
    UniGenei Hs.2962.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1J55 X-ray 2.00 A 1-95 [» ]
    1OZO NMR - A/B 1-95 [» ]
    ProteinModelPortali P25815.
    SMRi P25815. Positions 1-95.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112194. 15 interactions.
    IntActi P25815. 5 interactions.
    MINTi MINT-239013.
    STRINGi 9606.ENSP00000296370.

    Chemistry

    DrugBanki DB01003. Cromoglicate.

    PTM databases

    PhosphoSitei P25815.

    Polymorphism databases

    DMDMi 134142.

    Proteomic databases

    MaxQBi P25815.
    PaxDbi P25815.
    PeptideAtlasi P25815.
    PRIDEi P25815.

    Protocols and materials databases

    DNASUi 6286.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000296370 ; ENSP00000296370 ; ENSG00000163993 .
    GeneIDi 6286.
    KEGGi hsa:6286.
    UCSCi uc003gjl.3. human.

    Organism-specific databases

    CTDi 6286.
    GeneCardsi GC04P006698.
    HGNCi HGNC:10504. S100P.
    HPAi CAB012900.
    HPA019502.
    MIMi 600614. gene.
    neXtProti NX_P25815.
    PharmGKBi PA34913.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG78209.
    HOGENOMi HOG000246968.
    HOVERGENi HBG001479.
    InParanoidi P25815.
    OMAi LTSACHK.
    OrthoDBi EOG7R833W.
    PhylomeDBi P25815.
    TreeFami TF332727.

    Miscellaneous databases

    EvolutionaryTracei P25815.
    GeneWikii S100P.
    GenomeRNAii 6286.
    NextBioi 24409.
    PROi P25815.
    SOURCEi Search...

    Gene expression databases

    Bgeei P25815.
    CleanExi HS_S100P.
    Genevestigatori P25815.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR001751. S100/CaBP-9k_CS.
    IPR013787. S100_Ca-bd_sub.
    IPR028494. S100P.
    [Graphical view ]
    PANTHERi PTHR11639:SF69. PTHR11639:SF69. 1 hit.
    Pfami PF01023. S_100. 1 hit.
    [Graphical view ]
    PROSITEi PS50222. EF_HAND_2. 1 hit.
    PS00303. S100_CABP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "S100P, a novel Ca(2+)-binding protein from human placenta. cDNA cloning, recombinant protein expression and Ca2+ binding properties."
      Becker T., Gerke V., Kube E., Weber K.
      Eur. J. Biochem. 207:541-547(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Placenta.
    2. "Cloning, expression and characterization of a novel human calcium-binding S100 gene."
      Jin G., Wang S., Chen J.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Identification of a human migration-inducing gene 9 (MIG9)."
      Kim J.W.
      Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    6. "Purification and characterization of a new member of the S-100 protein family from human placenta."
      Emoto Y., Kobayashi R., Akatsuka H., Hidaka H.
      Biochem. Biophys. Res. Commun. 182:1246-1253(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-91.
      Tissue: Placenta.
    7. "Ca2+-dependent binding and activation of dormant ezrin by dimeric S100P."
      Koltzscher M., Neumann C., Konig S., Gerke V.
      Mol. Biol. Cell 14:2372-2384(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EZR.
    8. "Characterization of the tissue-specific expression of the s100P gene which encodes an EF-hand Ca2+-binding protein."
      Jin G., Wang S., Hu X., Jing Z., Chen J., Ying K., Xie Y., Mao Y.
      Mol. Biol. Rep. 30:243-248(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    9. Cited for: SUBUNIT, INTERACTION WITH S100A1.
    10. "S100P stimulates cell proliferation and survival via receptor for activated glycation end products (RAGE)."
      Arumugam T., Simeone D.M., Schmidt A.M., Logsdon C.D.
      J. Biol. Chem. 279:5059-5065(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. Cited for: INTERACTION WITH S100PBP, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    12. "Generation and characterization of a novel, permanently active S100P mutant."
      Austermann J., Nazmi A.R., Heil A., Fritz G., Kolinski M., Filipek S., Gerke V.
      Biochim. Biophys. Acta 1793:1078-1085(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH EZR, SUBCELLULAR LOCATION, MUTAGENESIS OF 21-SER--GLY-23; ASN-64; GLN-68 AND GLU-73.
    13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation."
      Yamaguchi F., Umeda Y., Shimamoto S., Tsuchiya M., Tokumitsu H., Tokuda M., Kobayashi R.
      J. Biol. Chem. 287:13787-13798(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PPP5C, MUTAGENESIS OF 21-SER--GLY-23; ASN-64; GLN-68 AND GLU-73.
    15. "The crystal structure at 2A resolution of the Ca2+ -binding protein S100P."
      Zhang H., Wang G., Ding Y., Wang Z., Barraclough R., Rudland P.S., Fernig D.G., Rao Z.
      J. Mol. Biol. 325:785-794(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    16. Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiS100P_HUMAN
    AccessioniPrimary (citable) accession number: P25815
    Secondary accession number(s): Q5J7W2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    This protein binds two calcium ions.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3