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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N7 position of guanine in position 535 of 16S rRNA.UniRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei82 – 821S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei147 – 1471S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBSUB:BSU41000-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.-UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Glucose-inhibited division protein B
Gene namesi
Name:rsmGUniRule annotation
Synonyms:gidB
Ordered Locus Names:BSU41000
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Low-level streptomycin resistance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Ribosomal RNA small subunit methyltransferase GPRO_0000184215Add
BLAST

Proteomic databases

PaxDbiP25813.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100022131.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1210Combined sources
Helixi19 – 3820Combined sources
Helixi47 – 537Combined sources
Helixi55 – 595Combined sources
Helixi60 – 634Combined sources
Helixi67 – 693Combined sources
Beta strandi72 – 765Combined sources
Beta strandi78 – 803Combined sources
Helixi84 – 907Combined sources
Beta strandi95 – 1017Combined sources
Helixi103 – 11614Combined sources
Beta strandi119 – 1268Combined sources
Helixi128 – 1314Combined sources
Turni135 – 1395Combined sources
Beta strandi141 – 1477Combined sources
Helixi152 – 1598Combined sources
Helixi160 – 1623Combined sources
Beta strandi163 – 17311Combined sources
Helixi178 – 19013Combined sources
Beta strandi193 – 20210Combined sources
Turni204 – 2063Combined sources
Beta strandi209 – 2179Combined sources
Helixi231 – 2344Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XDZX-ray1.60A1-239[»]
ProteinModelPortaliP25813.
SMRiP25813. Positions 1-238.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25813.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni128 – 1292S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221014.
InParanoidiP25813.
KOiK03501.
OMAiGVYLKHF.
OrthoDBiEOG6HF639.
PhylomeDBiP25813.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.

Sequencei

Sequence statusi: Complete.

P25813-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIEEFTSGL AEKGISLSPR QLEQFELYYD MLVEWNEKIN LTSITEKKEV
60 70 80 90 100
YLKHFYDSIT AAFYVDFNQV NTICDVGAGA GFPSLPIKIC FPHLHVTIVD
110 120 130 140 150
SLNKRITFLE KLSEALQLEN TTFCHDRAET FGQRKDVRES YDIVTARAVA
160 170 180 190 200
RLSVLSELCL PLVKKNGLFV ALKAASAEEE LNAGKKAITT LGGELENIHS
210 220 230
FKLPIEESDR NIMVIRKIKN TPKKYPRKPG TPNKSPIEG
Length:239
Mass (Da):26,954
Last modified:May 1, 1992 - v1
Checksum:i525826A929B32AA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62539 Genomic DNA. Translation: CAA44405.1.
D26185 Genomic DNA. Translation: BAA05230.1.
AL009126 Genomic DNA. Translation: CAB16137.1.
PIRiI40441. BWBSGB.
RefSeqiNP_391980.1. NC_000964.3.
WP_003243029.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB16137; CAB16137; BSU41000.
GeneIDi937931.
KEGGibsu:BSU41000.
PATRICi18980302. VBIBacSub10457_4309.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62539 Genomic DNA. Translation: CAA44405.1.
D26185 Genomic DNA. Translation: BAA05230.1.
AL009126 Genomic DNA. Translation: CAB16137.1.
PIRiI40441. BWBSGB.
RefSeqiNP_391980.1. NC_000964.3.
WP_003243029.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XDZX-ray1.60A1-239[»]
ProteinModelPortaliP25813.
SMRiP25813. Positions 1-238.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100022131.

Proteomic databases

PaxDbiP25813.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB16137; CAB16137; BSU41000.
GeneIDi937931.
KEGGibsu:BSU41000.
PATRICi18980302. VBIBacSub10457_4309.

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221014.
InParanoidiP25813.
KOiK03501.
OMAiGVYLKHF.
OrthoDBiEOG6HF639.
PhylomeDBiP25813.

Enzyme and pathway databases

BioCyciBSUB:BSU41000-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP25813.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genes and their organization in the replication origin region of the bacterial chromosome."
    Ogasawara N., Yoshikawa H.
    Mol. Microbiol. 6:629-634(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / CRK2000.
  2. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
    Ogasawara N., Nakai S., Yoshikawa H.
    DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants."
    Nishimura K., Johansen S.K., Inaoka T., Hosaka T., Tokuyama S., Tahara Y., Okamoto S., Kawamura F., Douthwaite S., Ochi K.
    J. Bacteriol. 189:6068-6073(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: 168.
  5. "The 1.6 A crystal structure of Gram-positive Bacillus subtilis glucose inhibited division protein b (gidB)."
    Midwest center for structural genomics (MCSG)
    Submitted (JAN-2005) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiRSMG_BACSU
AccessioniPrimary (citable) accession number: P25813
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: February 17, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.