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Protein

tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Gene

mnmG

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

Cofactori

FADUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei126 – 1261FAD; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei181 – 1811FADUniRule annotation
Binding sitei370 – 3701FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 196FADUniRule annotation
Nucleotide bindingi273 – 28715NADUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciBSUB:BSU41010-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmGUniRule annotation
Alternative name(s):
Glucose-inhibited division protein AUniRule annotation
Gene namesi
Name:mnmGUniRule annotation
Synonyms:gidAUniRule annotation
Ordered Locus Names:BSU41010
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 628628tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmGPRO_0000117054Add
BLAST

Proteomic databases

PaxDbiP25812.
PRIDEiP25812.

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Protein-protein interaction databases

IntActiP25812. 1 interaction.
MINTiMINT-8366300.
STRINGi224308.Bsubs1_010100022136.

Structurei

3D structure databases

ProteinModelPortaliP25812.
SMRiP25812. Positions 1-620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RE5. Bacteria.
COG0445. LUCA.
HOGENOMiHOG000201060.
InParanoidiP25812.
KOiK03495.
OMAiFRPGYAI.
PhylomeDBiP25812.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYEAGQYDV IVIGAGHAGV EAALASARQG AKTLVLTINL DMVAFMPCNP
60 70 80 90 100
SVGGPAKGIV VREIDALGGE MGRNIDKTHI QMRMLNTGKG PAVRALRAQA
110 120 130 140 150
DKFQYQHEMK NTLEKEPNLT LLQGIVERLI VEDGECRGVI TQTGAHYRAK
160 170 180 190 200
AVVMTTGTYL RGRIILGDLS YSSGPNNQQP SIKLSEHLEE LGFDLVRFKT
210 220 230 240 250
GTPPRVKSDT IDYSKTEIQP GDDVPRAFSY ETVEYITDQL PCWLTYTSPE
260 270 280 290 300
THEIIDSNLH RSPMYSGMIK GTGPRYCPSI EDKVVRFNDK PRHQIFLEPE
310 320 330 340 350
GRNTQEVYVQ GLSTSLPEDV QQRMLATIPG LENVQMMRAG YAIEYDAIVP
360 370 380 390 400
TQLWPTLETK KITNLYTAGQ INGTSGYEEA AGQGIMAGIN AGRKALGKEE
410 420 430 440 450
VILSRSDAYI GVLIDDLVTK GTNEPYRLLT SRAEYRLLLR HDNADLRLTE
460 470 480 490 500
IGHRIGLISD ERYAAFEKKK AAIEAEKKRL HSVIIKPSPE NQEYIRSLGG
510 520 530 540 550
SELKDGVRGT DLMKRPEMNY ETVTKLAPPE VPVPQDVAEQ VEIQVKYEGY
560 570 580 590 600
IEKSLQQVEK LKKMENKKIP DRIDYDAIKG IATEARQKLK NVRPLSVAQA
610 620
SRISGVNPAD ISILLVYLEQ GRIAKIAE
Length:628
Mass (Da):69,753
Last modified:May 1, 1992 - v1
Checksum:i63A62726BF723BAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62539 Genomic DNA. Translation: CAA44404.1.
D26185 Genomic DNA. Translation: BAA05231.1.
AL009126 Genomic DNA. Translation: CAB16138.1.
PIRiI40440. BWBSGA.
RefSeqiNP_391981.1. NC_000964.3.
WP_003226825.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB16138; CAB16138; BSU41010.
GeneIDi937932.
KEGGibsu:BSU41010.
PATRICi18980304. VBIBacSub10457_4310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62539 Genomic DNA. Translation: CAA44404.1.
D26185 Genomic DNA. Translation: BAA05231.1.
AL009126 Genomic DNA. Translation: CAB16138.1.
PIRiI40440. BWBSGA.
RefSeqiNP_391981.1. NC_000964.3.
WP_003226825.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliP25812.
SMRiP25812. Positions 1-620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP25812. 1 interaction.
MINTiMINT-8366300.
STRINGi224308.Bsubs1_010100022136.

Proteomic databases

PaxDbiP25812.
PRIDEiP25812.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB16138; CAB16138; BSU41010.
GeneIDi937932.
KEGGibsu:BSU41010.
PATRICi18980304. VBIBacSub10457_4310.

Phylogenomic databases

eggNOGiENOG4107RE5. Bacteria.
COG0445. LUCA.
HOGENOMiHOG000201060.
InParanoidiP25812.
KOiK03495.
OMAiFRPGYAI.
PhylomeDBiP25812.

Enzyme and pathway databases

BioCyciBSUB:BSU41010-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMNMG_BACSU
AccessioniPrimary (citable) accession number: P25812
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.