P25810 (GUR_GYMSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Gurmarin Alternative name(s): Sweet taste-suppressing peptide
Organism	Gymnema sylvestre (Gurmar)
Taxonomic identifier	4068 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Periplocoideae › Gymnema

Protein attributes

Sequence length	35 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Suppresses strongly the sweet taste responses in the rat with high specificity to sucrose, glucose, glycine, and saccharin. This effect is reversible, but complete recovery of the suppressed responses required at least 3h. Gurmarin showed no effect or only a very weak effect on the sweet taste sensation in humans.
Domain	The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Ontologies

Keywords
Domain	Knottin
Molecular function	Taste-modifying protein
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 35

35

Gurmarin

PRO_0000044776

Amino acid modifications

Modified residue

1

1

Pyrrolidone carboxylic acid Ref.1

Disulfide bond

3 ↔ 18

Ref.2 Ref.3 Ref.4

Disulfide bond

10 ↔ 23

Ref.2 Ref.3 Ref.4

Disulfide bond

17 ↔ 33

Ref.2 Ref.3 Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

35

Helix Strand Turn

Details...

Turn

12 – 14

3

Beta strand

18 – 20

3

Beta strand

23 – 29

7

Beta strand

31 – 33

3

Sequences

	Sequence		Length	Mass (Da)
	P25810 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: 661BD6FBAF504CB6	FASTA	35	4,232
	10 20 30 QQCVKKDELC IPYYLDCCEP LECKKVNWWD HKCIG

References

[1]	"Amino acid sequence of sweet-taste-suppressing peptide (gurmarin) from the leaves of Gymnema sylvestre." Kamei K., Takano R., Miyasaka A., Imoto T., Hara S. J. Biochem. 111:109-112(1992) [PubMed: 1607357] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Leaf.
[2]	"Location of the disulfide bonds of the sweetness-suppressing polypeptide gurmarin." Ota M., Ariyoshi Y. Biosci. Biotechnol. Biochem. 59:1956-1957(1995) [PubMed: 8534991] [Abstract] Cited for: DISULFIDE BONDS. Tissue: Leaf.
[3]	"Three-dimensional structure of gurmarin, a sweet taste-suppressing polypeptide." Arai K., Ishima R., Morikawa S., Miyasaka A., Imoto T., Yoshimura S., Aimoto S., Akasaka K. J. Biomol. NMR 5:297-305(1995) [PubMed: 7787425] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.
[4]	"High-resolution solution structure of gurmarin, a sweet-taste-suppressing plant polypeptide." Fletcher J.I., Dingley A.J., Smith R., Connor M., Christie M.J., King G.F. Eur. J. Biochem. 264:525-533(1999) [PubMed: 10491100] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Cross-references

Sequence databases

PIR

JX0200.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C4E	NMR	-	A	2-35	[»]
1GUR	NMR	-	A	2-35	[»]

ProteinModelPortal

P25810.

SMR

P25810. Positions 1-35.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR009101. Gurmarin/antifun_pep.
[Graphical view]

SUPFAM

SSF57048. Gurmarin-like. 1 hit.

ProtoNet

Search...

Other

PMAP-CutDB

P25810.

Entry information

Entry name

GUR_GYMSY

Accession

Primary (citable) accession number: P25810
Secondary accession number(s): Q9S8D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1994
Last modified:	November 30, 2010
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program