Reviewed, UniProtKB/Swiss-Prot P25809 (KCRU_RAT)

Names and origin

Protein names

Recommended name:
    Creatine kinase, ubiquitous mitochondrial
    EC=2.7.3.2
Alternative name(s):
    U-MtCK
    Acidic-type mitochondrial creatine kinase
      Short name=Mia-CK

Gene names

Name:	Ckmt1
Synonyms:	Ckmt

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Exists as an octamer composed of four MTCK homodimers.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.
Tissue specificity	In many tissues, with highest levels in brain gut and kidney. In the kidney localized primarily in the outer medulla in the thick ascending limb and distal convoluted tubule. Ref.2
Miscellaneous	Mitochondrial creatine kinase binds cardiolipin.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	phosphocreatine biosynthetic process Ref.1 Traceable author statement. Source: RGD
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Ref.1 Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion Ref.2
	Chain	40 – 418	379	Creatine kinase, ubiquitous mitochondrial		PRO_0000016592
Regions
	Nucleotide binding	162 – 166	5	ATP By similarity
	Nucleotide binding	354 – 359	6	ATP By similarity
	Region	40 – 64	25	Cardiolipin-binding By similarity
Sites
	Binding site	225	1	ATP By similarity
	Binding site	270	1	ATP By similarity
	Binding site	326	1	ATP By similarity
	Binding site	369	1	ATP By similarity
Amino acid modifications
	Modified residue	154	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25809-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 468339C52E4232D5	FASTA	418	47,029
	10 20 30 40 50 60 MAGPFSRLLS ARPGLKLLAL AGAGSLAAGI LLRPESVRAA TGERRRLYPP SAEYPDLRKH 70 80 90 100 110 120 NNCMASHLTP AVYARLCDKT TPTGWTLDQC IQTGVDNPGH PFIKTVGMVA GDEETYEVFA 130 140 150 160 170 180 ELFDPVIQER HNGYDPRTMK HTTDLDASKI RSGYFDERYV LSSRVRTGRS IRGLSLPPAC 190 200 210 220 230 240 TRAERREVER VVVDALSGLK GDLAGRYYRL SEMTEAEQQQ LIDDHFLFDK PVSPLLTAAG 250 260 270 280 290 300 MARDWPDARG IWHNNEKSFL IWVNEEDHTR VISMEKGGNM KRVFERFCRG LKKVEKLIQE 310 320 330 340 350 360 RGWEFMWNER LGYILTCPSN LGTGLRAGVH VKLPLLSKDS RFPKILENLR LQKRGTGGVD 370 380 390 400 410 TPATADVFDI SNLDRLGKSE VELVQLVIDG VNYLIDCERR LEKGQDIRIP PPLVHGKH

References

[1]	"Structural characterization and tissue-specific expression of the mRNAs encoding isoenzymes from two rat mitochondrial creatine kinase genes." Payne R.M., Haas R.C., Strauss A.W. Biochim. Biophys. Acta 1089:352-361(1991) [PubMed: 1859839] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Intestine.
[2]	"Compartmentation of multiple forms of creatine kinase in the distal nephron of the rat kidney." Friedman D.L., Perryman M.B. J. Biol. Chem. 266:22404-22410(1991) [PubMed: 1939264] [Abstract] Cited for: PROTEIN SEQUENCE OF 40-56, TISSUE SPECIFICITY.
[3]	Lubec G., Afjehi-Sadat L., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 152-158; 191-200; 258-270 AND 311-326, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59737 mRNA. Translation: CAA42415.1.
IPI	IPI00193445.
PIR	S17189.
UniGene	Rn.155589
3D structure databases
HSSP	HSSP built from PDB template 1QK1 based on UniProtKB P12532.
SMR	P25809. Positions 47-413.
ModBase	Search...
PTM databases
PhosphoSite	P25809.
Proteomic databases
PRIDE	P25809.
Genome annotation databases
Ensembl	ENSRNOG00000014573. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	61976. Ckmt1.
Phylogenomic databases
HOVERGEN	P25809.
Enzyme and pathway databases
BRENDA	2.7.3.2. 248.
Gene expression databases
ArrayExpress	P25809.
GermOnline	ENSRNOG00000014573. Rattus norvegicus.
Family and domain databases
InterPro	IPR000749. ATP-guanido_PTrfase. IPR014746. Gln_synth/guanido_kin_cat. [Graphical view]
Gene3D	G3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PANTHER	PTHR11547. ATP-gua_Ptrans. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
PROSITE	PS00112. GUANIDO_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCRU_RAT

Accession

Primary (citable) accession number: P25809

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)