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Protein

Creatine kinase U-type, mitochondrial

Gene

Ckmt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225ATPPROSITE-ProRule annotation1
Binding sitei270ATPPROSITE-ProRule annotation1
Binding sitei326ATPPROSITE-ProRule annotation1
Binding sitei369ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 166ATPPROSITE-ProRule annotation5
Nucleotide bindingi354 – 359ATPPROSITE-ProRule annotation6

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • creatine kinase activity Source: RGD
  • protein homodimerization activity Source: RGD

GO - Biological processi

  • brain development Source: RGD
  • cerebellum development Source: RGD
  • digestive tract development Source: RGD
  • kidney development Source: RGD
  • skeletal muscle tissue development Source: RGD

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase U-type, mitochondrial (EC:2.7.3.2)
Alternative name(s):
Acidic-type mitochondrial creatine kinase
Short name:
Mia-CK
Ubiquitous mitochondrial creatine kinase
Short name:
U-MtCK
Gene namesi
Name:Ckmt1
Synonyms:Ckmt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61976. Ckmt1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39Mitochondrion1 PublicationAdd BLAST39
ChainiPRO_000001659240 – 418Creatine kinase U-type, mitochondrialAdd BLAST379

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152PhosphoserineCombined sources1
Modified residuei197PhosphoserineCombined sources1
Modified residuei233PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP25809.
PRIDEiP25809.

2D gel databases

World-2DPAGEi0004:P25809.

PTM databases

iPTMnetiP25809.
PhosphoSitePlusiP25809.

Expressioni

Tissue specificityi

In many tissues, with highest levels in brain gut and kidney. In the kidney localized primarily in the outer medulla in the thick ascending limb and distal convoluted tubule.1 Publication

Interactioni

Subunit structurei

Exists as an octamer composed of four MTCK homodimers.

GO - Molecular functioni

  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044253.

Structurei

3D structure databases

ProteinModelPortaliP25809.
SMRiP25809.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 132Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST87
Domaini159 – 401Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 64Cardiolipin-bindingBy similarityAdd BLAST25

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP25809.
PhylomeDBiP25809.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiView protein in Pfam
PF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiView protein in PROSITE
PS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25809-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPFSRLLS ARPGLKLLAL AGAGSLAAGI LLRPESVRAA TGERRRLYPP
60 70 80 90 100
SAEYPDLRKH NNCMASHLTP AVYARLCDKT TPTGWTLDQC IQTGVDNPGH
110 120 130 140 150
PFIKTVGMVA GDEETYEVFA ELFDPVIQER HNGYDPRTMK HTTDLDASKI
160 170 180 190 200
RSGYFDERYV LSSRVRTGRS IRGLSLPPAC TRAERREVER VVVDALSGLK
210 220 230 240 250
GDLAGRYYRL SEMTEAEQQQ LIDDHFLFDK PVSPLLTAAG MARDWPDARG
260 270 280 290 300
IWHNNEKSFL IWVNEEDHTR VISMEKGGNM KRVFERFCRG LKKVEKLIQE
310 320 330 340 350
RGWEFMWNER LGYILTCPSN LGTGLRAGVH VKLPLLSKDS RFPKILENLR
360 370 380 390 400
LQKRGTGGVD TPATADVFDI SNLDRLGKSE VELVQLVIDG VNYLIDCERR
410
LEKGQDIRIP PPLVHGKH
Length:418
Mass (Da):47,029
Last modified:May 1, 1992 - v1
Checksum:i468339C52E4232D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59737 mRNA. Translation: CAA42415.1.
PIRiS17189.
UniGeneiRn.155589.

Genome annotation databases

UCSCiRGD:61976. rat.

Similar proteinsi

Entry informationi

Entry nameiKCRU_RAT
AccessioniPrimary (citable) accession number: P25809
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: August 30, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families