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Protein

Gut-specific cysteine proteinase

Gene

cpr-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Thiol protease. Has a role as a digestive enzyme.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei113 – 1131By similarity
Active sitei275 – 2751By similarity
Active sitei295 – 2951By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

ReactomeiR-CEL-1442490. Collagen degradation.
R-CEL-1679131. Trafficking and processing of endosomal TLR.

Protein family/group databases

MEROPSiC01.A32.

Names & Taxonomyi

Protein namesi
Recommended name:
Gut-specific cysteine proteinase (EC:3.4.22.-)
Gene namesi
Name:cpr-1
Synonyms:gcp-1
ORF Names:C52E4.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiC52E4.1; CE31896; WBGene00000781; cpr-1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence analysisAdd
BLAST
Propeptidei16 – 8469Activation peptideSequence analysisPRO_0000026190Add
BLAST
Chaini85 – 329245Gut-specific cysteine proteinasePRO_0000026191Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi98 ↔ 127By similarity
Disulfide bondi110 ↔ 155By similarity
Disulfide bondi146 ↔ 204By similarity
Disulfide bondi147 ↔ 151By similarity
Disulfide bondi183 ↔ 208By similarity
Disulfide bondi191 ↔ 196By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

EPDiP25807.
PaxDbiP25807.
PRIDEiP25807.

Expressioni

Tissue specificityi

Larvae exhibit strong expression in gut cells and weak expression in hypodermal cells. Adults exhibit the reverse: strong expression in hypodermal cells and weaker expression in gut cells.2 Publications

Developmental stagei

Larvae and adults, but not in embryos.2 Publications

Inductioni

Activated by a GATA-like transcription factor.

Interactioni

Protein-protein interaction databases

BioGridi44661. 2 interactions.
DIPiDIP-25619N.
MINTiMINT-1058393.
STRINGi6239.C52E4.1.

Structurei

3D structure databases

ProteinModelPortaliP25807.
SMRiP25807. Positions 33-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00780000121937.
HOGENOMiHOG000241341.
InParanoidiP25807.
KOiK01363.
OMAiCSLSCQS.
OrthoDBiEOG7034HR.
PhylomeDBiP25807.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25807-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLILTALC AVTLAFVPIN HQSAVETLTG QALVDYVNSA QSLFKTEHVE
60 70 80 90 100
ITEEEMKFKL MDGKYAAAHS DEIRATEQEV VLASVPATFD SRTQWSECKS
110 120 130 140 150
IKLIRDQATC GSCWAFGAAE MISDRTCIET KGAQQPIISP DDLLSCCGSS
160 170 180 190 200
CGNGCEGGYP IQALRWWDSK GVVTGGDYHG AGCKPYPIAP CTSGNCPESK
210 220 230 240 250
TPSCSMSCQS GYSTAYAKDK HFGVSAYAVP KNAASIQAEI YANGPVEAAF
260 270 280 290 300
SVYEDFYKYK SGVYKHTAGK YLGGHAIKII GWGTESGSPY WLVANSWGVN
310 320
WGESGFFKIY RGDDQCGIES AVVAGKAKV
Length:329
Mass (Da):35,397
Last modified:June 20, 2003 - v2
Checksum:i4FFD6F1B717B537D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74797 Genomic DNA. Translation: AAB88058.1.
Z78012 Genomic DNA. Translation: CAB01410.2.
PIRiT20148.
RefSeqiNP_506002.2. NM_073601.9.
UniGeneiCel.4328.

Genome annotation databases

EnsemblMetazoaiC52E4.1; C52E4.1; WBGene00000781.
GeneIDi179637.
KEGGicel:CELE_C52E4.1.
UCSCiC52E4.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74797 Genomic DNA. Translation: AAB88058.1.
Z78012 Genomic DNA. Translation: CAB01410.2.
PIRiT20148.
RefSeqiNP_506002.2. NM_073601.9.
UniGeneiCel.4328.

3D structure databases

ProteinModelPortaliP25807.
SMRiP25807. Positions 33-325.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi44661. 2 interactions.
DIPiDIP-25619N.
MINTiMINT-1058393.
STRINGi6239.C52E4.1.

Protein family/group databases

MEROPSiC01.A32.

Proteomic databases

EPDiP25807.
PaxDbiP25807.
PRIDEiP25807.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC52E4.1; C52E4.1; WBGene00000781.
GeneIDi179637.
KEGGicel:CELE_C52E4.1.
UCSCiC52E4.1. c. elegans.

Organism-specific databases

CTDi179637.
WormBaseiC52E4.1; CE31896; WBGene00000781; cpr-1.

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00780000121937.
HOGENOMiHOG000241341.
InParanoidiP25807.
KOiK01363.
OMAiCSLSCQS.
OrthoDBiEOG7034HR.
PhylomeDBiP25807.

Enzyme and pathway databases

ReactomeiR-CEL-1442490. Collagen degradation.
R-CEL-1679131. Trafficking and processing of endosomal TLR.

Miscellaneous databases

PROiP25807.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gut-specific and developmental expression of a Caenorhabditis elegans cysteine protease gene."
    Ray C., McKerrow J.H.
    Mol. Biochem. Parasitol. 51:239-250(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Regulation of the Caenorhabditis elegans gut cysteine protease gene cpr-1: requirement for GATA motifs."
    Britton C., McKerrow J.H., Johnstone I.L.
    J. Mol. Biol. 283:15-27(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCPR1_CAEEL
AccessioniPrimary (citable) accession number: P25807
Secondary accession number(s): Q18783
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 20, 2003
Last modified: June 8, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.