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Protein

Thiol protease

Gene

tpr

Organism
Porphyromonas gingivalis (strain ATCC BAA-308 / W83)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Thiol protease. Probably an important virulence factor.

Enzyme regulationi

Inactive below 20 degrees Celsius and pH 6.0. Inhibited by divalent cations.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei229 – 2291By similarity
Active sitei406 – 4061By similarity
Active sitei426 – 4261By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciPGIN242619:GHX8-956-MONOMER.
BRENDAi3.4.22.B8. 756.

Protein family/group databases

MEROPSiC02.022.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiol protease (EC:3.4.22.-)
Gene namesi
Name:tpr
Ordered Locus Names:PG_1055
OrganismiPorphyromonas gingivalis (strain ATCC BAA-308 / W83)
Taxonomic identifieri242619 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaePorphyromonas
Proteomesi
  • UP000000588 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Thiol proteasePRO_0000207747Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi242619.PG1055.

Structurei

3D structure databases

ProteinModelPortaliP25806.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini169 – 481313Calpain catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410Y3MW. LUCA.
KOiK08586.
OMAiINDREPH.
OrthoDBiEOG66MQR0.

Family and domain databases

InterProiIPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF00648. Peptidase_C2. 1 hit.
[Graphical view]
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKLVPQSI SKERLQKLEA QATLTPQQEE AKARKIEREK ARLKELNIPT
60 70 80 90 100
ESKESKDCSP AGMINPYALT EVILERPLDW SNPRTTDIVE RVLGSSMQDL
110 120 130 140 150
SKGDSVLRAG RDQNAEVKIV DSVLTKTQRG QDGLERILES FNDYDMPPEE
160 170 180 190 200
KEEAAPKAKK AAQKLDIDDL REQALSSTTI TKEISKIILP TKNLRDDNNT
210 220 230 240 250
VHQYREVGFQ SNGAHNLWDT VVQGIAGDCY MLAALSAIAW VWPALLNMDV
260 270 280 290 300
DIMSNQDEWR LYRYFIGRSK QTYARPSGSG TSTNEILQEG YYKVPIFARS
310 320 330 340 350
RYWFNGEYWP ALFEQAYANW KFPNDSKYNA ILQIGGGWPE EALCELSGDS
360 370 380 390 400
WFTSSGKLML SSFTDLSLLN FMKSMCYSWK TIKPMVIVTP CWEPLPPMMP
410 420 430 440 450
GIAAYHAYTV LGYTVSNGAY YLIIRNPWGV TEPTGDGVLS KRDWVIHFDN
460 470 480
MKWFNLSKDD GIFALRLDKV RENFWYIAYM Y
Length:481
Mass (Da):54,991
Last modified:October 3, 2003 - v2
Checksum:i9CC973EF01908383
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti274 – 2741A → AN in AAA25652 (PubMed:1322368).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84471 Genomic DNA. Translation: AAA25652.1.
AE015924 Genomic DNA. Translation: AAQ66170.1.
PIRiS27608.
RefSeqiWP_005873455.1. NC_002950.2.

Genome annotation databases

EnsemblBacteriaiAAQ66170; AAQ66170; PG_1055.
GeneIDi2553108.
KEGGipgi:PG1055.
PATRICi22979156. VBIPorGin134034_0973.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84471 Genomic DNA. Translation: AAA25652.1.
AE015924 Genomic DNA. Translation: AAQ66170.1.
PIRiS27608.
RefSeqiWP_005873455.1. NC_002950.2.

3D structure databases

ProteinModelPortaliP25806.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi242619.PG1055.

Protein family/group databases

MEROPSiC02.022.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAQ66170; AAQ66170; PG_1055.
GeneIDi2553108.
KEGGipgi:PG1055.
PATRICi22979156. VBIPorGin134034_0973.

Phylogenomic databases

eggNOGiENOG410Y3MW. LUCA.
KOiK08586.
OMAiINDREPH.
OrthoDBiEOG66MQR0.

Enzyme and pathway databases

BioCyciPGIN242619:GHX8-956-MONOMER.
BRENDAi3.4.22.B8. 756.

Family and domain databases

InterProiIPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF00648. Peptidase_C2. 1 hit.
[Graphical view]
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, and sequencing of a protease gene (tpr) from Porphyromonas gingivalis W83 in Escherichia coli."
    Bourgeau G., Lapointe H., Peloquin P., Mayrand D.
    Infect. Immun. 60:3186-3192(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-308 / W83.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-308 / W83.

Entry informationi

Entry nameiTPR_PORGI
AccessioniPrimary (citable) accession number: P25806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 3, 2003
Last modified: May 11, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.