P25803 (CYSEP_PHAVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Vignain EC=3.4.22.- Alternative name(s): Bean endopeptidase Cysteine proteinase EP-C1
Organism	Phaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifier	3885 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Thought to be involved in the hydrolysis of stored seed proteins.
Subunit structure	Monomer.
Subcellular location	Endoplasmic reticulum lumen.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Glycoprotein Zymogen
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 131	111	Activation peptide Potential		PRO_0000026440
	Chain	132 – 362	231	Vignain		PRO_0000026441
Regions
	Motif	359 – 362	4	Prevents secretion from ER Potential
Sites
	Active site	152	1	By similarity
	Active site	288	1	By similarity
	Active site	309	1	By similarity
Amino acid modifications
	Glycosylation	326	1	N-linked (GlcNAc...) Potential
	Glycosylation	346	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	107	1	P → H in CAA40073. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P25803 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 9AAF24A557FAF806	FASTA	362	40,212
	10 20 30 40 50 60 MATKKLLWVV LSFSLVLGVA NSFDFHDKDL ASEESLWDLY ERWRSHHTVS RSLGEKHKRF 70 80 90 100 110 120 NVFKANLMHV HNTNKMDKPY KLKLNKFADM TNHEFRSTYA GSKVNHPRMF RGTPHENGAF 130 140 150 160 170 180 MYEKVVSVPP SVDWRKKGAV TDVKDQGQCG SCWAFSTVVA VEGINQIKTN KLVALSEQEL 190 200 210 220 230 240 VDCDKEENQG CNGGLMESAF EFIKQKGGIT TESNYPYKAQ EGTCDASKVN DLAVSIDGHE 250 260 270 280 290 300 NVPANDEDAL LKAVANQPVS VAIDAGGSDF QFYSEGVFTG DCSTDLNHGV AIVGYGTTVD 310 320 330 340 350 360 GTNYWIVRNS WGPEWGEHGY IRMQRNISKK EGLCGIAMLP SYPIKNSSDN PTGSFSSPKD EL

References

[1]	"Nucleotide sequence of a gene for an endopeptidase (EP-C1) from Phaseolus vulgaris." Ogushi Y., Tanaka T., Yamauchi D., Minamikawa T. Plant Mol. Biol. 19:705-706(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Saxa. Tissue: Seedling.
[2]	"Nucleotide sequence of cDNA for an endopeptidase (EP-C1) from pods of maturing Phaseolus vulgaris fruits." Tanaka T., Yamauchi D., Minamikawa T. Plant Mol. Biol. 16:1083-1084(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-362. Strain: cv. Goldstar. Tissue: Fruit.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63102 Genomic DNA. Translation: CAA44816.1. X56753 mRNA. Translation: CAA40073.1.
PIR	S22502.
3D structure databases
ProteinModelPortal	P25803.
SMR	P25803. Positions 127-352.
ModBase	Search...
Protein family/group databases
MEROPS	I29.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR025661. Pept_asp_AS. IPR000169. Pept_cys_AS. IPR025660. Pept_his_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. PTHR12411. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00014. ER_TARGET. 1 hit. PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSEP_PHAVU

Accession

Primary (citable) accession number: P25803

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program