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P25803

- CYSEP_PHAVU

UniProt

P25803 - CYSEP_PHAVU

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Protein

Vignain

Gene
N/A
Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Thought to be involved in the hydrolysis of stored seed proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521By similarity
Active sitei288 – 2881By similarity
Active sitei309 – 3091By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Protein family/group databases

MEROPSiC01.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Vignain (EC:3.4.22.-)
Alternative name(s):
Bean endopeptidase
Cysteine proteinase EP-C1
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Propeptidei21 – 131111Activation peptideSequence AnalysisPRO_0000026440Add
BLAST
Chaini132 – 362231VignainPRO_0000026441Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence Analysis
Glycosylationi346 – 3461N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Zymogen

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP25803.
SMRiP25803. Positions 127-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi359 – 3624Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25803-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATKKLLWVV LSFSLVLGVA NSFDFHDKDL ASEESLWDLY ERWRSHHTVS
60 70 80 90 100
RSLGEKHKRF NVFKANLMHV HNTNKMDKPY KLKLNKFADM TNHEFRSTYA
110 120 130 140 150
GSKVNHPRMF RGTPHENGAF MYEKVVSVPP SVDWRKKGAV TDVKDQGQCG
160 170 180 190 200
SCWAFSTVVA VEGINQIKTN KLVALSEQEL VDCDKEENQG CNGGLMESAF
210 220 230 240 250
EFIKQKGGIT TESNYPYKAQ EGTCDASKVN DLAVSIDGHE NVPANDEDAL
260 270 280 290 300
LKAVANQPVS VAIDAGGSDF QFYSEGVFTG DCSTDLNHGV AIVGYGTTVD
310 320 330 340 350
GTNYWIVRNS WGPEWGEHGY IRMQRNISKK EGLCGIAMLP SYPIKNSSDN
360
PTGSFSSPKD EL
Length:362
Mass (Da):40,212
Last modified:June 1, 1994 - v2
Checksum:i9AAF24A557FAF806
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti107 – 1071P → H in CAA40073. (PubMed:1863761)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63102 Genomic DNA. Translation: CAA44816.1.
X56753 mRNA. Translation: CAA40073.1.
PIRiS22502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63102 Genomic DNA. Translation: CAA44816.1 .
X56753 mRNA. Translation: CAA40073.1 .
PIRi S22502.

3D structure databases

ProteinModelPortali P25803.
SMRi P25803. Positions 127-352.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C01.010.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view ]
PANTHERi PTHR12411. PTHR12411. 1 hit.
Pfami PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view ]
PRINTSi PR00705. PAPAIN.
SMARTi SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view ]
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of a gene for an endopeptidase (EP-C1) from Phaseolus vulgaris."
    Ogushi Y., Tanaka T., Yamauchi D., Minamikawa T.
    Plant Mol. Biol. 19:705-706(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Saxa.
    Tissue: Seedling.
  2. "Nucleotide sequence of cDNA for an endopeptidase (EP-C1) from pods of maturing Phaseolus vulgaris fruits."
    Tanaka T., Yamauchi D., Minamikawa T.
    Plant Mol. Biol. 16:1083-1084(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-362.
    Strain: cv. Goldstar.
    Tissue: Fruit.

Entry informationi

Entry nameiCYSEP_PHAVU
AccessioniPrimary (citable) accession number: P25803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3