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P25801

- RBTN2_MOUSE

UniProt

P25801 - RBTN2_MOUSE

Protein

Rhombotin-2

Gene

Lmo2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state.1 Publication

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. protein binding Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. embryonic hemopoiesis Source: MGI
    2. negative regulation of erythrocyte differentiation Source: UniProtKB
    3. positive regulation of transcription from RNA polymerase II promoter Source: MGI

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rhombotin-2
    Alternative name(s):
    Cysteine-rich protein TTG-2
    LIM domain only protein 2
    Short name:
    LMO-2
    T-cell translocation protein 2
    Gene namesi
    Name:Lmo2
    Synonyms:Rbtn-2, Rbtn2, Rhom-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:102811. Lmo2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB
    2. protein complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 158158Rhombotin-2PRO_0000075897Add
    BLAST

    Proteomic databases

    PRIDEiP25801.

    PTM databases

    PhosphoSiteiP25801.

    Expressioni

    Tissue specificityi

    Expressed in early mouse development in central nervous system, lung, kidney, liver and spleen but only very low levels occur in thymus.1 Publication

    Gene expression databases

    ArrayExpressiP25801.
    BgeeiP25801.
    CleanExiMM_LMO2.
    GenevestigatoriP25801.

    Interactioni

    Subunit structurei

    Interacts with BEX2 and KDM5A By similarity. Interacts via its LIM domains with ELF2 and LDB1. Also interacts with basic helix-loop-helix protein TAL1/SCL and can assemble in a complex with LMO2 and TAL1/SCL.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Gata1P176795EBI-3903256,EBI-3903251
    GATA2P237693EBI-3903256,EBI-2806671From a different organism.
    TAL1P175425EBI-3903256,EBI-1753878From a different organism.

    Protein-protein interaction databases

    BioGridi201179. 4 interactions.
    DIPiDIP-24247N.
    IntActiP25801. 5 interactions.
    MINTiMINT-2567948.

    Structurei

    Secondary structure

    1
    158
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi30 – 334
    Beta strandi39 – 446
    Beta strandi46 – 505
    Turni52 – 543
    Beta strandi58 – 603
    Beta strandi67 – 693
    Helixi81 – 877
    Beta strandi95 – 984
    Turni117 – 1193
    Beta strandi122 – 1254
    Beta strandi133 – 1375
    Beta strandi139 – 1435
    Beta strandi145 – 1473
    Helixi148 – 1558

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J2ONMR-A26-87[»]
    2L6YNMR-B84-156[»]
    2L6ZNMR-C84-156[»]
    2LXDNMR-A84-156[»]
    ProteinModelPortaliP25801.
    SMRiP25801. Positions 9-156.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP25801.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 8960LIM zinc-binding 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini94 – 15360LIM zinc-binding 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The second LIM zinc-binding domain interacts with KDM5A.By similarity

    Sequence similaritiesi

    Contains 2 LIM zinc-binding domains.PROSITE-ProRule annotation

    Keywords - Domaini

    LIM domain, Repeat

    Phylogenomic databases

    eggNOGiNOG319108.
    HOGENOMiHOG000232175.
    HOVERGENiHBG054231.
    InParanoidiP25801.
    KOiK15612.

    Family and domain databases

    Gene3Di2.10.110.10. 2 hits.
    InterProiIPR001781. Znf_LIM.
    [Graphical view]
    PfamiPF00412. LIM. 2 hits.
    [Graphical view]
    SMARTiSM00132. LIM. 2 hits.
    [Graphical view]
    PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
    PS50023. LIM_DOMAIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25801-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSAIERKSL DPSEEPVDEV LQIPPSLLTC GGCQQNIGDR YFLKAIDQYW    50
    HEDCLSCDLC GCRLGEVGRR LYYKLGRKLC RRDYLRLFGQ DGLCASCDKR 100
    IRAYEMTMRV KDKVYHLECF KCAACQKHFC VGDRYLLINS DIVCEQDIYE 150
    WTKINGII 158
    Length:158
    Mass (Da):18,340
    Last modified:May 1, 1992 - v1
    Checksum:i1B49302505528C93
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64360 mRNA. Translation: AAA40054.1.
    BC057880 mRNA. Translation: AAH57880.1.
    CCDSiCCDS50652.1.
    PIRiA39370.
    RefSeqiNP_001135808.1. NM_001142336.1.
    NP_001135809.1. NM_001142337.1.
    XP_006498883.1. XM_006498820.1.
    XP_006498884.1. XM_006498821.1.
    UniGeneiMm.29266.

    Genome annotation databases

    EnsembliENSMUST00000123437; ENSMUSP00000117703; ENSMUSG00000032698.
    ENSMUST00000163256; ENSMUSP00000129211; ENSMUSG00000032698.
    ENSMUST00000170926; ENSMUSP00000128317; ENSMUSG00000032698.
    GeneIDi16909.
    KEGGimmu:16909.
    UCSCiuc012caj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64360 mRNA. Translation: AAA40054.1 .
    BC057880 mRNA. Translation: AAH57880.1 .
    CCDSi CCDS50652.1.
    PIRi A39370.
    RefSeqi NP_001135808.1. NM_001142336.1.
    NP_001135809.1. NM_001142337.1.
    XP_006498883.1. XM_006498820.1.
    XP_006498884.1. XM_006498821.1.
    UniGenei Mm.29266.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1J2O NMR - A 26-87 [» ]
    2L6Y NMR - B 84-156 [» ]
    2L6Z NMR - C 84-156 [» ]
    2LXD NMR - A 84-156 [» ]
    ProteinModelPortali P25801.
    SMRi P25801. Positions 9-156.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201179. 4 interactions.
    DIPi DIP-24247N.
    IntActi P25801. 5 interactions.
    MINTi MINT-2567948.

    PTM databases

    PhosphoSitei P25801.

    Proteomic databases

    PRIDEi P25801.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000123437 ; ENSMUSP00000117703 ; ENSMUSG00000032698 .
    ENSMUST00000163256 ; ENSMUSP00000129211 ; ENSMUSG00000032698 .
    ENSMUST00000170926 ; ENSMUSP00000128317 ; ENSMUSG00000032698 .
    GeneIDi 16909.
    KEGGi mmu:16909.
    UCSCi uc012caj.1. mouse.

    Organism-specific databases

    CTDi 4005.
    MGIi MGI:102811. Lmo2.

    Phylogenomic databases

    eggNOGi NOG319108.
    HOGENOMi HOG000232175.
    HOVERGENi HBG054231.
    InParanoidi P25801.
    KOi K15612.

    Miscellaneous databases

    ChiTaRSi LMO2. mouse.
    EvolutionaryTracei P25801.
    NextBioi 290948.
    PROi P25801.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P25801.
    Bgeei P25801.
    CleanExi MM_LMO2.
    Genevestigatori P25801.

    Family and domain databases

    Gene3Di 2.10.110.10. 2 hits.
    InterProi IPR001781. Znf_LIM.
    [Graphical view ]
    Pfami PF00412. LIM. 2 hits.
    [Graphical view ]
    SMARTi SM00132. LIM. 2 hits.
    [Graphical view ]
    PROSITEi PS00478. LIM_DOMAIN_1. 2 hits.
    PS50023. LIM_DOMAIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The rhombotin family of cysteine-rich LIM-domain oncogenes: distinct members are involved in T-cell translocations to human chromosomes 11p15 and 11p13."
      Boehm T., Foroni L., Kaneko Y., Perutz M.F., Rabbitts T.H.
      Proc. Natl. Acad. Sci. U.S.A. 88:4367-4371(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Embryo.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NMRI.
      Tissue: Mammary gland.
    3. "Elf-2, a rhombotin-2 binding ets transcription factor: discovery and potential role in T cell leukemia."
      Wilkinson D.A., Neale G.A.M., Mao S., Naeve C.W., Goorha R.M.
      Leukemia 11:86-96(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ELF2.
    4. "The LIM-domain binding protein Ldb1 and its partner LMO2 act as negative regulators of erythroid differentiation."
      Visvader J.E., Mao X., Fujiwara Y., Hahm K., Orkin S.H.
      Proc. Natl. Acad. Sci. U.S.A. 94:13707-13712(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LDB1 AND TAL1, IDENTIFICATION IN A COMPLEX WITH LDB1 AND TAL1.
    5. "Structural basis for the recognition of ldb1 by the N-terminal LIM domains of LMO2 and LMO4."
      Deane J.E., Mackay J.P., Kwan A.H.Y., Sum E.Y.M., Visvader J.E., Matthews J.M.
      EMBO J. 22:2224-2233(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 26-87 IN COMPLEX WITH LDB1.

    Entry informationi

    Entry nameiRBTN2_MOUSE
    AccessioniPrimary (citable) accession number: P25801
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3