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P25801 (RBTN2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rhombotin-2
Alternative name(s):
Cysteine-rich protein TTG-2
LIM domain only protein 2
Short name=LMO-2
T-cell translocation protein 2
Gene names
Name:Lmo2
Synonyms:Rbtn-2, Rbtn2, Rhom-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length158 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts with TAL1/SCL to regulate red blood cell development. Also acts with LDB1 to maintain erythroid precursors in an immature state. Ref.4

Subunit structure

Interacts with BEX2 and KDM5A By similarity. Interacts via its LIM domains with ELF2 and LDB1. Also interacts with basic helix-loop-helix protein TAL1/SCL and can assemble in a complex with LMO2 and TAL1/SCL. Ref.3 Ref.4

Subcellular location

Nucleus Ref.4.

Tissue specificity

Expressed in early mouse development in central nervous system, lung, kidney, liver and spleen but only very low levels occur in thymus. Ref.1

Domain

The second LIM zinc-binding domain interacts with KDM5A By similarity.

Sequence similarities

Contains 2 LIM zinc-binding domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Gata1P176795EBI-3903256,EBI-3903251
GATA2P237693EBI-3903256,EBI-2806671From a different organism.
TAL1P175425EBI-3903256,EBI-1753878From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 158158Rhombotin-2
PRO_0000075897

Regions

Domain30 – 8960LIM zinc-binding 1
Domain94 – 15360LIM zinc-binding 2

Secondary structure

............................ 158
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25801 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 1B49302505528C93

FASTA15818,340
        10         20         30         40         50         60 
MSSAIERKSL DPSEEPVDEV LQIPPSLLTC GGCQQNIGDR YFLKAIDQYW HEDCLSCDLC 

        70         80         90        100        110        120 
GCRLGEVGRR LYYKLGRKLC RRDYLRLFGQ DGLCASCDKR IRAYEMTMRV KDKVYHLECF 

       130        140        150 
KCAACQKHFC VGDRYLLINS DIVCEQDIYE WTKINGII 

« Hide

References

« Hide 'large scale' references
[1]"The rhombotin family of cysteine-rich LIM-domain oncogenes: distinct members are involved in T-cell translocations to human chromosomes 11p15 and 11p13."
Boehm T., Foroni L., Kaneko Y., Perutz M.F., Rabbitts T.H.
Proc. Natl. Acad. Sci. U.S.A. 88:4367-4371(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Embryo.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NMRI.
Tissue: Mammary gland.
[3]"Elf-2, a rhombotin-2 binding ets transcription factor: discovery and potential role in T cell leukemia."
Wilkinson D.A., Neale G.A.M., Mao S., Naeve C.W., Goorha R.M.
Leukemia 11:86-96(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ELF2.
[4]"The LIM-domain binding protein Ldb1 and its partner LMO2 act as negative regulators of erythroid differentiation."
Visvader J.E., Mao X., Fujiwara Y., Hahm K., Orkin S.H.
Proc. Natl. Acad. Sci. U.S.A. 94:13707-13712(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LDB1 AND TAL1, IDENTIFICATION IN A COMPLEX WITH LDB1 AND TAL1.
[5]"Structural basis for the recognition of ldb1 by the N-terminal LIM domains of LMO2 and LMO4."
Deane J.E., Mackay J.P., Kwan A.H.Y., Sum E.Y.M., Visvader J.E., Matthews J.M.
EMBO J. 22:2224-2233(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 26-87 IN COMPLEX WITH LDB1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64360 mRNA. Translation: AAA40054.1.
BC057880 mRNA. Translation: AAH57880.1.
PIRA39370.
RefSeqNP_001135808.1. NM_001142336.1.
NP_001135809.1. NM_001142337.1.
XP_006498883.1. XM_006498820.1.
XP_006498884.1. XM_006498821.1.
UniGeneMm.29266.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1J2ONMR-A26-87[»]
2L6YNMR-B84-156[»]
2L6ZNMR-C84-156[»]
2LXDNMR-A84-156[»]
ProteinModelPortalP25801.
SMRP25801. Positions 26-156.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201179. 4 interactions.
DIPDIP-24247N.
IntActP25801. 5 interactions.
MINTMINT-2567948.

PTM databases

PhosphoSiteP25801.

Proteomic databases

PRIDEP25801.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000123437; ENSMUSP00000117703; ENSMUSG00000032698.
ENSMUST00000163256; ENSMUSP00000129211; ENSMUSG00000032698.
ENSMUST00000170926; ENSMUSP00000128317; ENSMUSG00000032698.
GeneID16909.
KEGGmmu:16909.
UCSCuc012caj.1. mouse.

Organism-specific databases

CTD4005.
MGIMGI:102811. Lmo2.

Phylogenomic databases

eggNOGNOG319108.
HOGENOMHOG000232175.
HOVERGENHBG054231.
InParanoidP25801.
KOK15612.

Gene expression databases

ArrayExpressP25801.
BgeeP25801.
CleanExMM_LMO2.
GenevestigatorP25801.

Family and domain databases

Gene3D2.10.110.10. 2 hits.
InterProIPR001781. Znf_LIM.
[Graphical view]
PfamPF00412. LIM. 2 hits.
[Graphical view]
SMARTSM00132. LIM. 2 hits.
[Graphical view]
PROSITEPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSLMO2. mouse.
EvolutionaryTraceP25801.
NextBio290948.
PROP25801.
SOURCESearch...

Entry information

Entry nameRBTN2_MOUSE
AccessionPrimary (citable) accession number: P25801
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: March 19, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot