P25798 (FLIF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flagellar M-ring protein

Gene names

Name:	fliF
Synonyms:	fla AII.1, fla BI
Ordered Locus Names:	b1938, JW1922

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The M ring may be actively involved in energy transduction.
Subunit structure	The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The M ring is integral to the inner membrane of the cell and may be connected to the flagellar rod via the S ring. The S (supramembrane ring) lies just distal to the M ring. The L and P rings lie in the outer membrane and the periplasmic space, respectively.
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body Ref.7.
Sequence similarities	Belongs to the fliF family.

Ontologies

Keywords
Cellular component	Bacterial flagellum Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ciliary or flagellar motility Inferred from electronic annotation. Source: InterPro
Cellular component	bacterial-type flagellum basal body, MS ring Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	motor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Flagellar M-ring protein		PRO_0000180882
Regions
	Transmembrane	23 – 43	21	Helical; Potential
	Transmembrane	447 – 467	21	Helical; Potential
Experimental info
	Sequence conflict	260	1	H → R in BAA14029. Ref.1
	Sequence conflict	266	1	Q → H in BAA14029. Ref.1
	Sequence conflict	541	1	L → V Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P25798 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: B2038FDBEA914DA5	FASTA	552	60,589
	10 20 30 40 50 60 MNATAAQTKS LEWLNRLRAN PKIPLIVAGS AAVAVMVALI LWAKAPDYRT LFSNLSDQDG 70 80 90 100 110 120 GAIVSQLTQM NIPYRFSEAS GAIEVPADKV HELRLRLAQQ GLPKGGAVGF ELLDQEKFGI 130 140 150 160 170 180 SQFSEQVNYQ RALEGELSRT IETIGPVKGA RVHLAMPKPS LFVREQKSPS ASVTVNLLPG 190 200 210 220 230 240 RALDEGQISA IVHLVSSAVA GLPPGNVTLV DQGGHLLTQS NTSGRDLNDA QLKYASDVEG 250 260 270 280 290 300 RIQRRIEAIL SPIVGNGNIH AQVTAQLDFA SKEQTEEQYR PNGDESHAAL RSRQLNESEQ 310 320 330 340 350 360 SGSGYPGGVP GALSNQPAPA NNAPISTPPA NQNNRQQQAS TTSNSGPRST QRNETSNYEV 370 380 390 400 410 420 DRTIRHTKMN VGDVQRLSVA VVVNYKTLPD GKPLPLSNEQ MKQIEDLTRE AMGFSEKRGD 430 440 450 460 470 480 SLNVVNSPFN SSDESGGELP FWQQQAFIDQ LLAAGRWLLV LLVAWLLWRK AVRPQLTRRA 490 500 510 520 530 540 EAMKAVQQQA QAREEVEDAV EVRLSKDEQL QQRRANQRLG AEVMSQRIRE MSDNDPRVVA 550 LVIRQWINND HE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	al Mamun A.A.M., Tominaga A., Enomoto M. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Characterization of the fliE genes of Escherichia coli and Salmonella typhimurium and identification of the FliE protein as a component of the flagellar hook-basal body complex." Mueller V., Jones C.J., Kawagishi I., Aizawa S., Macnab R.M. J. Bacteriol. 174:2298-2304(1992) [PubMed: 1551848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5. Strain: JA11.
[6]	"Gene sequence, overproduction, purification and determination of the wild-type level of the Escherichia coli flagellar switch protein FliG." Roman S.J., Frantz B.B., Matsumura P. Gene 133:103-108(1993) [PubMed: 8224881] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 532-552.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D89826 Genomic DNA. Translation: BAA14029.1. U00096 Genomic DNA. Translation: AAC75005.1. AP009048 Genomic DNA. Translation: BAA15763.1. M84992 Genomic DNA. No translation available. L13243 Genomic DNA. No translation available.
PIR	G64957.
RefSeq	NP_416448.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P25798.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-401N.
IntAct	P25798. 4 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000800; EBESCP00000000800; EBESCG00000000669. EBESCT00000000801; EBESCP00000000801; EBESCG00000000669. EBESCT00000016882; EBESCP00000016173; EBESCG00000015941.
GeneID	946448.
GenomeReviews	Gene locus JW1922 in contig AP009048_GR. Gene locus b1938 in contig U00096_GR.
KEGG	ecj:JW1922. eco:b1938.
PATRIC	32119205. VBIEscCol129921_2017.
Organism-specific databases
EchoBASE	EB1323.
EcoGene	EG11347. fliF.
Phylogenomic databases
eggNOG	COG1766.
GeneTree	EBGT00050000011524.
HOGENOM	HBG661951.
OMA	FMEATRY.
PhylomeDB	P25798.
ProtClustDB	PRK06007.
Enzyme and pathway databases
BioCyc	EcoCyc:FLIF-FLAGELLAR-MS-RING.
Gene expression databases
Genevestigator	P25798.
Family and domain databases
InterPro	IPR013556. Flag_M-ring_C. IPR000067. FlgMring_FLIF. IPR006182. YscJ_FliF. [Graphical view]
KO	K02409.
Pfam	PF01514. YscJ_FliF. 1 hit. PF08345. YscJ_FliF_C. 1 hit. [Graphical view]
PRINTS	PR01009. FLGMRINGFLIF.
TIGRFAMs	TIGR00206. FliF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FLIF_ECOLI

Accession

Primary (citable) accession number: P25798
Secondary accession number(s): P76324, P76914

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program