Reviewed, UniProtKB/Swiss-Prot P25796 (CATE_CAVPO)

Names and origin

Protein names

Recommended name:
    Cathepsin E
    EC=3.4.23.34

Gene names

Name:

CTSE

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain By similarity.
Catalytic activity	Similar to cathepsin D, but slightly broader specificity. Ref.1
Subunit structure	Homodimer; disulfide-linked. Ref.1
Subcellular location	Endosome By similarity. Note: The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome By similarity.
Tissue specificity	Expressed abundantly in the surface and foveolar epithelial cells of the fundic and pyloric stomach mucosa, and at very low levels in the spleen. Ref.1 Ref.3
Post-translational modification	Glycosylated. The nature of the carbohydrate chain varies between cell types By similarity.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Endosome
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	antigen processing and presentation of exogenous peptide antigen via MHC class II Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	endosome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	aspartic-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.1
	Propeptide	20 – 53	34	Activation peptide Potential		PRO_0000025972
	Chain	54 – 391	338	Cathepsin E		PRO_0000025973
Sites
	Active site	92	1	By similarity
	Active site	276	1	By similarity
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Disulfide bond	56		Interchain Probable
	Disulfide bond	105 ↔ 110		By similarity
	Disulfide bond	267 ↔ 271		By similarity
	Disulfide bond	309 ↔ 346		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25796-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 78D216BF8CFCDABD	FASTA	391	42,132
	10 20 30 40 50 60 MKTFLLLLLV LLELGQAPGA LHRVPLSRRE SLRKKLRAQG QLTELWKSQN LNMDQCSTIQ 70 80 90 100 110 120 SANEPLINYL DMEYFGTISI GSPPQNFTVI FDTGSSNLWV PSVYCTSPAC QTHPVFHPSL 130 140 150 160 170 180 SSTYREVGNS FSIQYGTGSL TGIIGADQVS VEGLTVVGQQ FGESVQEPGK TFVHAEFDGI 190 200 210 220 230 240 LGLGYPSLAA GGVTPVFDNM MAQNLVALPM FSVYMSSNPG GSGSELTFGG YDPSHFSGSL 250 260 270 280 290 300 NWVPVTKQAY WQIALDGIQV GDSVMFCSEG CQAIVDTGTS LITGPPGKIK QLQEALGATY 310 320 330 340 350 360 VDEGYSVQCA NLNMMLDVTF IINGVPYTLN PTAYTLLDFV DGMQVCSTGF EGLEIQPPAG 370 380 390 PLWILGDVFI RQFYAVFDRG NNRVGLAPAV P

References

[1]	"Gastric procathepsin E and progastricsin from guinea pig. Purification, molecular cloning of cDNAs, and characterization of enzymatic properties, with special reference to procathepsin E." Kageyama T., Ichinose M., Tsukada S., Miki K., Kurokawa K., Koiwai O., Tanji M., Yakabe E., Athauda S.B., Takahashi K. J. Biol. Chem. 267:16450-16459(1992) [PubMed: 1644829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-48, CATALYTIC ACTIVITY, SUBUNIT, TISSUE SPECIFICITY.
[2]	"Isolation, characterization, and structure of procathepsin E and cathepsin E from the gastric mucosa of guinea pig." Kageyama T., Ichinose M., Miki K., Moriyama A., Yonezawa S., Tanji M., Athauda S.B., Takahashi K. Adv. Exp. Med. Biol. 362:211-221(1995) [PubMed: 8540321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Gastric mucosa.
[3]	"Tissue- and cell-specific control of guinea pig cathepsin E gene expression." Tsukada S., Ichinose M., Miki K., Tatematsu M., Yonezawa S., Matsushima M., Kakei N., Fukamachi H., Yasugi S., Kurokawa K., Kageyama T., Takahashi K. Biochem. Biophys. Res. Commun. 187:1401-1408(1992) [PubMed: 1417816] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M88653 mRNA. Translation: AAA37052.1. S80547 mRNA. Translation: AAB35844.1.
PIR	A43356.
3D structure databases
HSSP	HSSP built from PDB template 4CMS based on UniProtKB P00794.
SMR	P25796. Positions 64-390.
ModBase	Search...
Protein family/group databases
MEROPS	A01.010.
Genome annotation databases
Ensembl	ENSCPOT00000009609; ENSCPOP00000008547; ENSCPOG00000009523; Cavia porcellus. [Genome view]
Phylogenomic databases
HOVERGEN	P25796.
OMA	TAYTLLD.
Enzyme and pathway databases
BRENDA	3.4.23.34. 44.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
PMAP-CutDB	P25796.

Entry information

Entry name

CATE_CAVPO

Accession

Primary (citable) accession number: P25796

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)