Reviewed, UniProtKB/Swiss-Prot P25783 (CATV_NPVAC)

Names and origin

Protein names

Recommended name:
    Viral cathepsin
      Short name=V-cath
    EC=3.4.22.50
Alternative name(s):
    Cysteine proteinase
      Short name=CP

Gene names

Name:

VCATH

Organism

Autographa californica nuclear polyhedrosis virus (AcMNPV)

Taxonomic identifier

46015 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Baculoviridae › Alphabaculovirus

Virus host

Lepidoptera (butterflies and moths) [TaxID: 7088]

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cysteine protease that plays an essential role in host liquefaction to facilitate horizontal transmission of the virus. May participate in the degradation of foreign protein expressed by the baculovirus system By similarity.
Catalytic activity	Endopeptidase of broad specificity, hydrolyzing substrates of both cathepsin L and cathepsin B.
Post-translational modification	Synthesized as an inactive proenzyme and activated by proteolytic removal of the inhibitory propeptide.
Sequence similarities	Belongs to the peptidase C1 family.
Biophysicochemical properties	pH dependence: Optimum pH is 5.0-5.5.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 112	96	Activation peptide Potential		PRO_0000322201
	Chain	113 – 323	211	Viral cathepsin		PRO_0000050572
Sites
	Active site	136	1	By similarity
	Active site	269	1	By similarity
	Active site	289	1	By similarity
Amino acid modifications
	Glycosylation	158	1	N-linked (GlcNAc...); by host Potential
	Disulfide bond	133 ↔ 174		By similarity
	Disulfide bond	167 ↔ 207		By similarity
	Disulfide bond	262 ↔ 310		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25783-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: F23876A6ED303FC8	FASTA	323	36,938
	10 20 30 40 50 60 MNKILFYLFV YGVVNSAAYD LLKAPNYFEE FVHRFNKDYG SEVEKLRRFK IFQHNLNEII 70 80 90 100 110 120 NKNQNDSAKY EINKFSDLSK DETIAKYTGL SLPIQTQNFC KVIVLDQPPG KGPLEFDWRR 130 140 150 160 170 180 LNKVTSVKNQ GMCGACWAFA TLASLESQFA IKHNQLINLS EQQMIDCDFV DAGCNGGLLH 190 200 210 220 230 240 TAFEAIIKMG GVQLESDYPY EADNNNCRMN SNKFLVQVKD CYRYITVYEE KLKDLLRLVG 250 260 270 280 290 300 PIPMAIDAAD IVNYKQGIIK YCFNSGLNHA VLLVGYGVEN NIPYWTFKNT WGTDWGEDGF 310 320 FRVQQNINAC GMRNELASTA VIY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a cysteine proteinase encoded by the Autographa californica nuclear polyhedrosis virus." Kuzio J., Faulkner P. (In) Goosen M.F.A., Daugulis A.J., Faulkner P. (eds.); Insect cell culture engineering, pp.17-50, Marcel Dekker, New York (1993) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C6.
[2]	"The complete DNA sequence of Autographa californica nuclear polyhedrosis virus." Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D. Virology 202:586-605(1994) [PubMed: 8030224] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C6.
[3]	"The baculovirus Autographa californica nuclear polyhedrosis virus genome includes a papain-like sequence." Rawlings N.D., Pearl L.H., Buttle D.J. Biol. Chem. Hoppe-Seyler 373:1211-1215(1992) [PubMed: 1363350] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 118-323.
[4]	"The baculovirus cysteine protease has a cathepsin B-like S2-subsite specificity." Bromme D., Okamoto K. Biol. Chem. Hoppe-Seyler 376:611-615(1995) [PubMed: 8590630] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Characterization of v-cath, a cathepsin L-like proteinase expressed by the baculovirus Autographa californica multiple nuclear polyhedrosis virus." Slack J.M., Kuzio J., Faulkner P. J. Gen. Virol. 76:1091-1098(1995) [PubMed: 7730794] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Autographa californica M nucleopolyhedrovirus ProV-CATH is activated during infected cell death." Hom L.G., Ohkawa T., Trudeau D., Volkman L.E. Virology 296:212-218(2002) [PubMed: 12069520] [Abstract] Cited for: PROTEOLYTIC CLEAVAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M67451 Genomic DNA. Translation: AAA46752.1. L22858 Genomic DNA. Translation: AAA66757.1. X70124 Genomic DNA. Translation: CAA49713.1. Different initiation.
PIR	S62736.
RefSeq	NP_054157.1.
3D structure databases
HSSP	HSSP built from PDB template 1K3B based on UniProtKB P53634.
ModBase	Search...
Protein family/group databases
MEROPS	C01.083.
Genome annotation databases
GeneID	1403960.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATV_NPVAC

Accession

Primary (citable) accession number: P25783
Secondary accession number(s): Q96589

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 5, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)