Reviewed, UniProtKB/Swiss-Prot P25780 (PEPT1_EURMA)

Names and origin

Protein names

Recommended name:
    Peptidase 1
    EC=3.4.22.65
Alternative name(s):
    Mite group 1 allergen Eur m 1
    Allergen Eur m I
    Allergen=Eur m 1

Gene names

Name:

EURM1

Organism

Euroglyphus maynei (Mayne's house dust mite)

Taxonomic identifier

6958 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Acari › Acariformes › Sarcoptiformes › Astigmata › Psoroptidia › Analgoidea › Pyroglyphidae › Pyroglyphidae incertae sedis › Euroglyphus

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable thiol protease.
Catalytic activity	Broad endopeptidase specificity.
Subcellular location	Secreted.
Allergenic properties	Causes an allergic reaction in human. Common symptoms of mite allergy are bronchial asthma, allergic rhinitis and conjunctivitis.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 98	80			PRO_0000026378
	Chain	99 – 321	223	Peptidase 1		PRO_0000026379
Sites
	Active site	133	1	By similarity
	Active site	269	1	By similarity
	Active site	289	1	By similarity
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Disulfide bond	130 ↔ 170		By similarity
Natural variations
	Natural variant	36	1	T → S in Eur m 1.0102.
	Natural variant	126	1	M → N in Eur m 1.0102.
	Natural variant	320	1	M → I in Eur m 1.0102.

Sequences

	Sequence		Length	Mass (Da)
	P25780-1 [UniParc]. Last modified June 20, 2001. Version 2. Checksum: 6CFD44FEC725999E	FASTA	321	36,290
	10 20 30 40 50 60 MKIILAIASL LVLSAVYARP ASIKTFEEFK KAFNKTYATP EKEEVARKNF LESLKYVESN 70 80 90 100 110 120 KGAINHLSDL SLDEFKNQFL MNANAFEQLK TQFDLNAETY ACSINSVSLP SELDLRSLRT 130 140 150 160 170 180 VTPIRMQGGC GSCWAFSGVA STESAYLAYR NMSLDLAEQE LVDCASQNGC HGDTIPRGIE 190 200 210 220 230 240 YIQQNGVVQE HYYPYVAREQ SCHRPNAQRY GLKNYCQISP PDSNKIRQAL TQTHTAVAVI 250 260 270 280 290 300 IGIKDLNAFR HYDGRTIMQH DNGYQPNYHA VNIVGYGNTQ GVDYWIVRNS WDTTWGDNGY 310 320 GYFAANINLM MIEQYPYVVM L

References

[1]	"Molecular analysis of the group 1 and 2 allergens from the house dust mite, Euroglyphus maynei." Smith W., Mills K., Hazell L., Hart B.J., Thomas W. Int. Arch. Allergy Immunol. 118:15-22(1999) [PubMed: 9925958] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (EUR M 1.0101 AND EUR M 1.0102).
[2]	"Molecular characterisation of group I allergen Eur m I from house dust mite Euroglyphus maynei." Kent N.A., Hill M.R., Keen J.N., Holland P.W., Hart B.J. Int. Arch. Allergy Immunol. 99:150-152(1992) [PubMed: 1483062] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-309.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF047610 mRNA. Translation: AAC82351.1. AF047611 mRNA. Translation: AAC82352.1. Different initiation. AF047612 mRNA. Translation: AAC82353.1. X60073 Genomic DNA. Translation: CAA42677.1.
PIR	S21864.
3D structure databases
HSSP	HSSP built from PDB template 1K3B based on UniProtKB P53634.
SMR	P25780. Positions 22-321.
ModBase	Search...
Protein family/group databases
MEROPS	C01.073.
Enzyme and pathway databases
BRENDA	3.4.22.65. 295922.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPT1_EURMA

Accession

Primary (citable) accession number: P25780
Secondary accession number(s): Q9TZZ3, Q9TZZ4, Q9UBA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	June 20, 2001
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)