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Protein

DNA ligase B

Gene

ligB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction.1 Publication

Miscellaneous

Considerably less active in nick joining than LigA.

Catalytic activityi

NAD+ + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.

Cofactori

Mg2+1 Publication, Mn2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei124N6-AMP-lysine intermediateCurated1

GO - Molecular functioni

  • DNA ligase (NAD+) activity Source: EcoCyc

GO - Biological processi

  • base-excision repair, DNA ligation Source: GO_Central
  • DNA ligation Source: EcoCyc
  • DNA replication Source: UniProtKB-KW

Keywordsi

Molecular functionLigase
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Manganese, NAD

Enzyme and pathway databases

BioCyciEcoCyc:EG11334-MONOMER
MetaCyc:EG11334-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase B (EC:6.5.1.2)
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD(+)] B
Gene namesi
Name:ligB
Synonyms:yicF
Ordered Locus Names:b3647, JW3622
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11334 ligB

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi124K → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001617761 – 560DNA ligase BAdd BLAST560

Proteomic databases

PaxDbiP25772
PRIDEiP25772

Interactioni

Protein-protein interaction databases

BioGridi4262567, 167 interactors
IntActiP25772, 3 interactors
STRINGi316385.ECDH10B_3829

Structurei

3D structure databases

ProteinModelPortaliP25772
SMRiP25772
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410649J Bacteria
COG0272 LUCA
HOGENOMiHOG000267776
KOiK01972
OMAiLFVWDWP
PhylomeDBiP25772

Family and domain databases

CDDicd00114 LIGANc, 1 hit
HAMAPiMF_01587 DNA_ligase_B, 1 hit
InterProiView protein in InterPro
IPR018239 DNA_ligase_AS
IPR020923 DNA_ligase_B
IPR033136 DNA_ligase_CS
IPR013839 DNAligase_adenylation
IPR013840 DNAligase_N
IPR012340 NA-bd_OB-fold
IPR004150 NAD_DNA_ligase_OB
IPR010994 RuvA_2-like
PfamiView protein in Pfam
PF01653 DNA_ligase_aden, 1 hit
PF03120 DNA_ligase_OB, 1 hit
SMARTiView protein in SMART
SM00532 LIGANc, 1 hit
SUPFAMiSSF47781 SSF47781, 1 hit
SSF50249 SSF50249, 1 hit
PROSITEiView protein in PROSITE
PS01055 DNA_LIGASE_N1, 1 hit
PS01056 DNA_LIGASE_N2, 1 hit

Sequencei

Sequence statusi: Complete.

P25772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVWMAILIG ILCWQSSVWA VCPAWSPARA QEEISRLQQQ IKQWDDDYWK
60 70 80 90 100
EGKSEVEDGV YDQLSARLTQ WQRCFGSEPR DVMMPPLNGA VMHPVAHTGV
110 120 130 140 150
RKMVDKNALS LWMRERSDLW VQPKVDGVAV TLVYRDGKLN KAISRGNGLK
160 170 180 190 200
GEDWTQKVSL ISAVPQTVSG PLANSTLQGE IFLQREGHIQ QQMGGINARA
210 220 230 240 250
KVAGLMMRQD DSDTLNSLGV FVWAWPDGPQ LMSDRLKELA TAGFTLTQTY
260 270 280 290 300
TRAVKNADEV ARVRNEWWKA ELPFVTDGVV VRAAKEPESR HWLPGQAEWL
310 320 330 340 350
VAWKYQPVAQ VAEVKAIQFA VGKSGKISVV ASLAPVMLDD KKVQRVNIGS
360 370 380 390 400
VRRWQEWDIA PGDQILVSLA GQGIPRIDDV VWRGAERTKP TPPENRFNSL
410 420 430 440 450
TCYFASDVCQ EQFISRLVWL GAKQVLGLDG IGEAGWRALH QTHRFEHIFS
460 470 480 490 500
WLLLTPEQLQ NTPGIAKSKS AQLWHQFNLA RKQPFTRWVM AMGIPLTRAA
510 520 530 540 550
LNASDERSWS QLLFSTEQFW QQLPGTGSGR ARQVIEWKEN AQIKKLGSWL
560
AAQQITGFEP
Length:560
Mass (Da):63,179
Last modified:July 1, 1993 - v2
Checksum:i2C1BA1F9B247A599
GO

Sequence cautioni

The sequence AAA62000 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAB88710 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10328 Genomic DNA Translation: AAA62000.1 Different initiation.
U00096 Genomic DNA Translation: AAC76671.2
AP009048 Genomic DNA Translation: BAE77646.1
M84400 Genomic DNA Translation: AAB88710.1 Different initiation.
PIRiA65166
RefSeqiNP_418104.2, NC_000913.3
WP_000870036.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76671; AAC76671; b3647
BAE77646; BAE77646; BAE77646
GeneIDi948164
KEGGiecj:JW3622
eco:b3647
PATRICifig|511145.12.peg.3767

Similar proteinsi

Entry informationi

Entry nameiLIGB_ECOLI
AccessioniPrimary (citable) accession number: P25772
Secondary accession number(s): P78120, Q2M7W0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: July 1, 1993
Last modified: March 28, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
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