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Protein

Chitinase 12

Gene

Cht12

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Its overexpression confers enhanced resistance to sheath blight pathogen (R.solani).1 Publication

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  • beta-N-acetylglucosaminidase activity Source: Gramene
  • chitinase activity Source: UniProtKB
  • chitin binding Source: Gramene
  • endochitinase activity Source: Gramene

GO - Biological processi

  • amino sugar metabolic process Source: Gramene
  • cell wall macromolecule catabolic process Source: InterPro
  • chitin catabolic process Source: UniProtKB-KW
  • defense response to fungus Source: UniProtKB
  • polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase 12 (EC:3.2.1.14)
Alternative name(s):
Basic endochitinase 2
Pathogenesis related (PR)-3 chitinase 12
Gene namesi
Name:Cht12
Synonyms:RCH10
Ordered Locus Names:Os03g0418000, LOC_Os03g30470
ORF Names:OSJNBb0028K20.4, OSJNBb0056B16.17
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 3

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 326305Chitinase 12PRO_0000005306Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi24 ↔ 39PROSITE-ProRule annotation
Disulfide bondi33 ↔ 45PROSITE-ProRule annotation
Disulfide bondi36 ↔ 65PROSITE-ProRule annotation
Disulfide bondi38 ↔ 52PROSITE-ProRule annotation
Disulfide bondi56 ↔ 60PROSITE-ProRule annotation
Disulfide bondi102 ↔ 165PROSITE-ProRule annotation
Disulfide bondi179 ↔ 187PROSITE-ProRule annotation
Disulfide bondi286 ↔ 318PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP25765.

Expressioni

Tissue specificityi

Expressed in meristems and at lower levels in roots and sheaths.1 Publication

Gene expression databases

ExpressionAtlasiP25765. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os03g30470.1.

Structurei

3D structure databases

ProteinModelPortaliP25765.
SMRiP25765. Positions 22-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 6241Chitin-binding type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000231411.
InParanoidiP25765.
OMAiANLDCYS.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25765-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRALAVVAMV ATAFLAAAVH AEQCGSQAGG AVCPNCLCCS QFGWCGSTSD
60 70 80 90 100
YCGAGCQSQC SAAGCGGGGP TPPSGSGGSG VASIVSRSLF DQMLLHRNDA
110 120 130 140 150
ACPASNFYTY DAFVAAASAF PGFAAAGGDA DTNKREVAAF LAQTSHETTG
160 170 180 190 200
GWATAPDGPY AWGYCFKEEN GGAAGPDYCQ QSAQWPCAAG KKYYGRGPIQ
210 220 230 240 250
LSYNFNYGPA GQAIGADLLG DPDLVASDAT VSFDTAFWFW MTPQSPKPSC
260 270 280 290 300
HAVATGQWTP SADDQAAGRV PGYGVITNII NGGLECGHGE DDRVADRIGF
310 320
YKRYCDILGV SYDANLDCYS QRPFGS
Length:326
Mass (Da):33,636
Last modified:September 5, 2006 - v2
Checksum:i41E748706FAFEDC1
GO

Sequence cautioni

The sequence described in PubMed:2034221 differs from that shown.Sequencing errors.Curated
The sequence described in PubMed:2034221 differs from that shown. Reason: Frameshift at several positions. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137992 Genomic DNA. Translation: AAP44624.1.
AC145386 Genomic DNA. Translation: AAR01697.1.
DP000009 Genomic DNA. Translation: ABF96639.1.
AP008209 Genomic DNA. Translation: BAF12287.2.
AP014959 Genomic DNA. Translation: BAS84717.1.
PIRiS15997.
RefSeqiXP_015629397.1. XM_015773911.1.

Genome annotation databases

EnsemblPlantsiOS03T0418000-00; OS03T0418000-00; OS03G0418000.
GeneIDi4333121.
GrameneiOS03T0418000-00; OS03T0418000-00; OS03G0418000.
KEGGiosa:4333121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC137992 Genomic DNA. Translation: AAP44624.1.
AC145386 Genomic DNA. Translation: AAR01697.1.
DP000009 Genomic DNA. Translation: ABF96639.1.
AP008209 Genomic DNA. Translation: BAF12287.2.
AP014959 Genomic DNA. Translation: BAS84717.1.
PIRiS15997.
RefSeqiXP_015629397.1. XM_015773911.1.

3D structure databases

ProteinModelPortaliP25765.
SMRiP25765. Positions 22-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os03g30470.1.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PaxDbiP25765.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS03T0418000-00; OS03T0418000-00; OS03G0418000.
GeneIDi4333121.
GrameneiOS03T0418000-00; OS03T0418000-00; OS03G0418000.
KEGGiosa:4333121.

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000231411.
InParanoidiP25765.
OMAiANLDCYS.

Gene expression databases

ExpressionAtlasiP25765. baseline and differential.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a rice gene encoding a basic chitinase."
    Zhu Q., Lamb C.J.
    Mol. Gen. Genet. 226:289-296(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  6. "Co-expression of a modified maize ribosome-inactivating protein and a rice basic chitinase gene in transgenic rice plants confers enhanced resistance to sheath blight."
    Kim J.-K., Jang I.-C., Wu R., Zuo W.-N., Boston R.S., Lee Y.-H., Ahn I.-P., Nahm B.H.
    Transgenic Res. 12:475-484(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
    Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
    Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCHI12_ORYSJ
AccessioniPrimary (citable) accession number: P25765
Secondary accession number(s): A0A0P0VYS9, Q0DR51, Q7Y1K8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 5, 2006
Last modified: June 8, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.