P25765 (CHI12_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitinase 12
EC=3.2.1.14

Alternative name(s):
Basic endochitinase 2
Pathogenesis related (PR)-3 chitinase 12

Gene names

Name:	Cht12
Synonyms:	RCH10
Ordered Locus Names:	Os03g0418000, LOC_Os03g30470
ORF Names:	OSJNBb0028K20.4, OSJNBb0056B16.17

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Its overexpression confers enhanced resistance to sheath blight pathogen (R.solani). Ref.5
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Tissue specificity	Expressed in meristems and at lower levels in roots and sheaths. Ref.6
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.
Sequence caution	The sequence described in Ref.1 differs from that shown. Reason: Frameshift at several positions. The sequence described in Ref.1 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	amino sugar metabolic process Inferred from direct assay. Source: Gramene cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response to fungus Inferred from direct assay Ref.5. Source: UniProtKB
Molecular function	beta-N-acetylglucosaminidase activity Inferred from direct assay. Source: Gramene chitin binding Inferred from electronic annotation. Source: UniProtKB-KW endochitinase activity Inferred from direct assay. Source: Gramene
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 326	305	Chitinase 12		PRO_0000005306
Regions
	Domain	22 – 62	41	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	24 ↔ 39		By similarity
	Disulfide bond	33 ↔ 45		By similarity
	Disulfide bond	36 ↔ 65		By similarity
	Disulfide bond	38 ↔ 52		By similarity
	Disulfide bond	56 ↔ 60		By similarity
	Disulfide bond	102 ↔ 165		By similarity
	Disulfide bond	179 ↔ 187		By similarity
	Disulfide bond	286 ↔ 318		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25765 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: 41E748706FAFEDC1	FASTA	326	33,636
	10 20 30 40 50 60 MRALAVVAMV ATAFLAAAVH AEQCGSQAGG AVCPNCLCCS QFGWCGSTSD YCGAGCQSQC 70 80 90 100 110 120 SAAGCGGGGP TPPSGSGGSG VASIVSRSLF DQMLLHRNDA ACPASNFYTY DAFVAAASAF 130 140 150 160 170 180 PGFAAAGGDA DTNKREVAAF LAQTSHETTG GWATAPDGPY AWGYCFKEEN GGAAGPDYCQ 190 200 210 220 230 240 QSAQWPCAAG KKYYGRGPIQ LSYNFNYGPA GQAIGADLLG DPDLVASDAT VSFDTAFWFW 250 260 270 280 290 300 MTPQSPKPSC HAVATGQWTP SADDQAAGRV PGYGVITNII NGGLECGHGE DDRVADRIGF 310 320 YKRYCDILGV SYDANLDCYS QRPFGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a rice gene encoding a basic chitinase." Zhu Q., Lamb C.J. Mol. Gen. Genet. 226:289-296(1991) [PubMed: 2034221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"Co-expression of a modified maize ribosome-inactivating protein and a rice basic chitinase gene in transgenic rice plants confers enhanced resistance to sheath blight." Kim J.-K., Jang I.-C., Wu R., Zuo W.-N., Boston R.S., Lee Y.-H., Ahn I.-P., Nahm B.H. Transgenic Res. 12:475-484(2003) [PubMed: 12885168] [Abstract] Cited for: FUNCTION.
[6]	"Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)." Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T. Genome 49:619-630(2006) [PubMed: 16936841] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC137992 Genomic DNA. Translation: AAP44624.1. AC145386 Genomic DNA. Translation: AAR01697.1. DP000009 Genomic DNA. Translation: ABF96639.1. AP008209 Genomic DNA. Translation: BAF12287.2.
PIR	S15997.
RefSeq	NP_001050373.2. NM_001056908.2.
UniGene	Os.79080.
3D structure databases
ProteinModelPortal	P25765.
SMR	P25765. Positions 22-324.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g30470.1; LOC_Os03g30470.1; LOC_Os03g30470.
GeneID	4333121.
KEGG	osa:4333121.
Organism-specific databases
Gramene	P25765.
Phylogenomic databases
OMA	SCHAVAT.
PhylomeDB	P25765.
Family and domain databases
InterPro	IPR001002. Chitin-bd_1. IPR018371. Chitin-binding_1_CS. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. IPR023346. Lysozyme-like_dom. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
SUPFAM	SSF57016. Chitin_bd_1. 1 hit. SSF53955. SSF53955. 1 hit.
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI12_ORYSJ

Accession

Primary (citable) accession number: P25765
Secondary accession number(s): Q0DR51, Q7Y1K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	September 5, 2006
Last modified:	December 14, 2011
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program