Reviewed,
UniProtKB/Swiss-Prot P25765 (CHI2_ORYSJ)
Last modified
June 16, 2009.
Version 73.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Basic endochitinase 2 EC=3.2.1.14 | ||||||
| Gene names |
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| Organism | Oryza sativa subsp. japonica (Rice) | ||||||
| Taxonomic identifier | 39947 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza |
Protein attributes
| Sequence length | 326 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Defense against chitin containing fungal pathogens. |
| Catalytic activity | Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. |
| Sequence similarities | Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain. |
| Sequence caution | The sequence described in Ref.1 differs from that shown. Reason: Frameshift at several positions. The sequence described in Ref.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation |
| Domain | Signal |
| Ligand | Chitin-binding |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond |
| Gene Ontology (GO) | |
| Biological process | cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic processInferred from electronic annotation. Source: UniProtKB-KW defense responseInferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | beta-N-acetylglucosaminidase activity Ref.1 Inferred from direct assay. Source: Gramene chitin binding Ref.1Inferred from expression pattern. Source: Gramene endochitinase activity Ref.1Inferred from direct assay. Source: Gramene |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 21 | 21 | Potential | ||||||||
| Chain | 22 – 326 | 305 | Basic endochitinase 2 | PRO_0000005306 | |||||||
Regions | |||||||||||
| Domain | 22 – 62 | 41 | Chitin-binding type-1 | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 24 ↔ 39 | By similarity | |||||||||
| Disulfide bond | 33 ↔ 45 | By similarity | |||||||||
| Disulfide bond | 38 ↔ 52 | By similarity | |||||||||
| Disulfide bond | 56 ↔ 60 | By similarity | |||||||||
| Disulfide bond | 102 ↔ 165 | By similarity | |||||||||
| Disulfide bond | 179 ↔ 187 | By similarity | |||||||||
| Disulfide bond | 286 ↔ 318 | By similarity | |||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation and characterization of a rice gene encoding a basic chitinase." Zhu Q., Lamb C.J. Mol. Gen. Genet. 226:289-296(1991) [PubMed: 2034221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. Jackson S.Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
Cross-references
Sequence databases | |
|---|---|
| AC137992 Genomic DNA. Translation: AAP44624.1. AC145386 Genomic DNA. Translation: AAR01697.1. DP000009 Genomic DNA. Translation: ABF96639.1. | |
| PIR | S15997. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1CNS based on UniProtKB P23951. |
| SMR | P25765. Positions 22-324. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19. |
Organism-specific databases | |
| Gramene | P25765. |
Family and domain databases | |
| InterPro | IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view] |
| Gene3D | G3DSA:3.30.60.10. Chitin_bd_1. 1 hit. |
| PANTHER | PTHR22595. Glyco_hydro_19_cat. 1 hit. |
| Pfam | PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view] |
| PIRSF | PIRSF001060. Endochitinase. 1 hit. |
| PRINTS | PR00451. CHITINBINDNG. |
| ProDom | PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00270. ChtBD1. 1 hit. [Graphical view] |
| PROSITE | PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CHI2_ORYSJ | ||||||||
| Accession | Primary (citable) accession number: P25765 Secondary accession number(s): Q7Y1K8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| Oryza sativa (rice) Index of Oryza sativa entries and their corresponding gene designations |
| SIMILARITY comments Index of protein domains and families |

Clusters with


