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Reviewed, UniProtKB/Swiss-Prot P25765 (CHI12_ORYSJ)

Last modified February 9, 2010. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Chitinase 12
    EC=3.2.1.14
Alternative name(s):
    Pathogenesis related (PR)-3 chitinase 12
    Basic endochitinase 2
Gene names
Name: Cht12
Synonyms: RCH10
Ordered Locus Names: Os03g0418000, LOC_Os03g30470
ORF Names: OSJNBb0028K20.4, OSJNBb0056B16.17
OrganismOryza sativa subsp. japonica (Rice)
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Its overexpression confers enhanced resistance to sheath blight pathogen (R.solani). Ref.5

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Tissue specificity

Expressed in meristems and at lower levels in roots and sheaths. Ref.6

Sequence similarities

Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.

Contains 1 chitin-binding type-1 domain.

Sequence caution

The sequence BAF12287.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence described in Ref.1 differs from that shown. Reason: Frameshift at several positions.

The sequence described in Ref.1 differs from that shown. Reason: Miscellaneous discrepancy. Sequencing errors.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 326305Chitinase 12
PRO_0000005306

Regions

Domain22 – 6241Chitin-binding type-1

Amino acid modifications

Disulfide bond24 ↔ 39 By similarity
Disulfide bond33 ↔ 45 By similarity
Disulfide bond36 ↔ 65 By similarity
Disulfide bond38 ↔ 52 By similarity
Disulfide bond56 ↔ 60 By similarity
Disulfide bond102 ↔ 165 By similarity
Disulfide bond179 ↔ 187 By similarity
Disulfide bond286 ↔ 318 By similarity

Sequences

Sequence LengthMass (Da)Tools
P25765-1 [UniParc].

Last modified September 5, 2006. Version 2.
Checksum: 41E748706FAFEDC1

FASTA32633,636
        10         20         30         40         50         60 
MRALAVVAMV ATAFLAAAVH AEQCGSQAGG AVCPNCLCCS QFGWCGSTSD YCGAGCQSQC 

        70         80         90        100        110        120 
SAAGCGGGGP TPPSGSGGSG VASIVSRSLF DQMLLHRNDA ACPASNFYTY DAFVAAASAF 

       130        140        150        160        170        180 
PGFAAAGGDA DTNKREVAAF LAQTSHETTG GWATAPDGPY AWGYCFKEEN GGAAGPDYCQ 

       190        200        210        220        230        240 
QSAQWPCAAG KKYYGRGPIQ LSYNFNYGPA GQAIGADLLG DPDLVASDAT VSFDTAFWFW 

       250        260        270        280        290        300 
MTPQSPKPSC HAVATGQWTP SADDQAAGRV PGYGVITNII NGGLECGHGE DDRVADRIGF 

       310        320 
YKRYCDILGV SYDANLDCYS QRPFGS 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of a rice gene encoding a basic chitinase."
Zhu Q., Lamb C.J.
Mol. Gen. Genet. 226:289-296(1991) [PubMed: 2034221] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed: 16100779] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana."
The rice annotation project (RAP)
Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"Co-expression of a modified maize ribosome-inactivating protein and a rice basic chitinase gene in transgenic rice plants confers enhanced resistance to sheath blight."
Kim J.-K., Jang I.-C., Wu R., Zuo W.-N., Boston R.S., Lee Y.-H., Ahn I.-P., Nahm B.H.
Transgenic Res. 12:475-484(2003) [PubMed: 12885168] [Abstract]
Cited for: FUNCTION.
[6]"Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
Genome 49:619-630(2006) [PubMed: 16936841] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC137992 Genomic DNA. Translation: AAP44624.1.
AC145386 Genomic DNA. Translation: AAR01697.1.
DP000009 Genomic DNA. Translation: ABF96639.1.
AP008209 Genomic DNA. Translation: BAF12287.1. Sequence problems.
PIRS15997.
RefSeqNP_001050373.1.
UniGeneOs.79080

3D structure databases

SMRP25765. Positions 22-324.
ModBaseSearch...

Protein family/group databases

CAZyCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Genome annotation databases

GeneID4333121.
GenomeReviewsGene locus Cht12 in contig AP008209_GR.
KEGGosa:4333121.

Organism-specific databases

GrameneP25765.

Family and domain databases

InterProIPR018371. Chitin-binding_1_CS.
IPR001002. Chitin_bd_1.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
[Graphical view]
Gene3DG3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHERPTHR22595. Glyco_hydro_19_cat. 1 hit.
PfamPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFPIRSF001060. Endochitinase. 1 hit.
PRINTSPR00451. CHITINBINDNG.
ProDomPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00270. ChtBD1. 1 hit.
[Graphical view]
PROSITEPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHI12_ORYSJ
AccessionPrimary (citable) accession number: P25765
Secondary accession number(s): Q0DR51, Q7Y1K8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 5, 2006
Last modified: February 9, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents