P25750 (CH60_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60 kDa chaperonin

Alternative name(s):
GroEL protein
Protein Cpn60
Symbionin

Gene names

Name:	groL
Synonyms:	groEL, mopA, symL
Ordered Locus Names:	BU019

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions By similarity. HAMAP-Rule MF_00600
Subunit structure	Oligomer of 14 subunits composed of two stacked rings of 7 subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00600.
Sequence similarities	Belongs to the chaperonin (HSP60) family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein refolding Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	60 kDa chaperonin HAMAP-Rule MF_00600		PRO_0000063305

Sequences

	Sequence		Length	Mass (Da)
	P25750 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 9B33AE1E629E7592	FASTA	548	57,911
	10 20 30 40 50 60 MAAKDVKFGN EARIKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPSIT KDGVSVAREI 70 80 90 100 110 120 ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATLLAQSIV NEGLKAVAAG MNPMDLKRGI 130 140 150 160 170 180 DKAVISAVEE LKHLSVPCSD SKAITQVGTI SANADEKVGS LIAEAMEKVG NDGVITVEEG 190 200 210 220 230 240 TGLQDELEVV KGMQFDRGYL SPYFINKPET GIVELENPYI LMADKKISNV REMLPILESV 250 260 270 280 290 300 AKSGKPLLII SEDLEGEALA TLVVNSMRGI VKVAAVKAPG FGDRRKAMLQ DISILTGGSV 310 320 330 340 350 360 ISEELAMELE KSTLEDLGQA KRVVISKDTT TIIGGVGEKH SIQSRISQIR QEIQEATSDY 370 380 390 400 410 420 DKEKLNERLA KLSGGVAVLK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALV 430 440 450 460 470 480 RVAGKIADLR GQNEDQNVGI RVALRAMEAP LRQIVSNSGE EPSVVTNNVK DGKGNYGYNA 490 500 510 520 530 540 ATDEYGDMID FGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKEDKS SDSSSSPAGG MGGMGGMM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structures of chaperonins from an intracellular symbiont and their functional expression in Escherichia coli groE mutants." Ohtaka C., Nakamura H., Ishikawa H. J. Bacteriol. 174:1869-1874(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X61150 Genomic DNA. Translation: CAA43460.1. BA000003 Genomic DNA. Translation: BAB12746.1.
RefSeq	NP_239860.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P25750.
SMR	P25750. Positions 2-526.
ModBase	Search...
Protein-protein interaction databases
STRING	107806.BU019.
Proteomic databases
PRIDE	P25750.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12746; BAB12746; BAB12746.
GeneID	1109527.
KEGG	buc:BU019.
PATRIC	21243542. VBIBucAph127364_0031.
Phylogenomic databases
eggNOG	COG0459.
HOGENOM	HOG000076290.
KO	K04077.
OMA	VEDAMHA.
ProtClustDB	PRK00013.
Enzyme and pathway databases
BioCyc	BAPH107806:GBZJ-19-MONOMER.
Family and domain databases
Gene3D	1.10.560.10. 2 hits. 3.50.7.10. 1 hit.
HAMAP	MF_00600. CH60.
InterPro	IPR018370. Chaperonin_Cpn60_CS. IPR001844. Chaprnin_Cpn60. IPR002423. Cpn60/TCP-1. IPR027409. GroEL-like_apical_dom. IPR027413. GROEL-like_equatorial. [Graphical view]
PANTHER	PTHR11353. PTHR11353. 1 hit.
Pfam	PF00118. Cpn60_TCP1. 1 hit. [Graphical view]
PRINTS	PR00298. CHAPERONIN60.
SUPFAM	SSF48592. GroEL-ATPase. 1 hit. SSF52029. SSF52029. 1 hit.
TIGRFAMs	TIGR02348. GroEL. 1 hit.
PROSITE	PS00296. CHAPERONINS_CPN60. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CH60_BUCAI

Accession

Primary (citable) accession number: P25750

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 29, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program