Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

HTH-type transcriptional regulator GalS

Gene

galS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of the mgl operon. Binds galactose and D-fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence (corresponding to residues 15 to 20).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi4 – 2320H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • transcription factor activity, sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00261.
ECOL316407:JW2138-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator GalS
Alternative name(s):
Mgl repressor and galactose ultrainduction factor
Gene namesi
Name:galS
Ordered Locus Names:b2151, JW2138
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10365. galS.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346HTH-type transcriptional regulator GalSPRO_0000107956Add
BLAST

Proteomic databases

PaxDbiP25748.
PRIDEiP25748.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
galRP030245EBI-1117006,EBI-556902

Protein-protein interaction databases

DIPiDIP-9734N.
IntActiP25748. 7 interactions.
STRINGi511145.b2151.

Structurei

3D structure databases

ProteinModelPortaliP25748.
SMRiP25748. Positions 2-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5656HTH lacI-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH lacI-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41060NJ. Bacteria.
COG1609. LUCA.
HOGENOMiHOG000220179.
InParanoidiP25748.
KOiK02529.
OMAiTGSPDMQ.
OrthoDBiEOG6SJJMM.
PhylomeDBiP25748.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR000843. HTH_LacI.
IPR010982. Lambda_DNA-bd_dom.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00356. LacI. 1 hit.
[Graphical view]
PRINTSiPR00036. HTHLACI.
SMARTiSM00354. HTH_LACI. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF53822. SSF53822. 1 hit.
PROSITEiPS00356. HTH_LACI_1. 1 hit.
PS50932. HTH_LACI_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITIRDVARQ AGVSVATVSR VLNNSTLVSA DTREAVMKAV SELDYRPNAN
60 70 80 90 100
AQALATQVSD TIGVVVMDVS DAFFGALVKA VDLVAQQHQK YVLIGNSYHE
110 120 130 140 150
AEKERHAIEV LIRQRCNALI VHSKALSDDE LAQFMDNIPG MVLINRVVPG
160 170 180 190 200
YAHRCVCLDN LSGARMATRM LLNNGHQRIG YLSSSHGIED DAMRKAGWMS
210 220 230 240 250
ALKEQDIIPP ESWIGAGTPD MPGGEAAMVE LLGRNLQLTA VFAYNDNMAA
260 270 280 290 300
GALTALKDNG IAIPLHLSII GFDDIPIARY TDPQLTTVRY PIASMAKLAT
310 320 330 340
ELALQGAAGN IDPRASHCFM PTLVRRHSVA TRQNAAAITN STNQAM
Length:346
Mass (Da):37,357
Last modified:November 1, 1997 - v2
Checksum:iF13D9DF41A29ADDA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1052ER → DG in CAA44390 (PubMed:1619663).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62529 Genomic DNA. Translation: CAA44390.1.
U00007 Genomic DNA. Translation: AAA60528.1.
U00096 Genomic DNA. Translation: AAC75212.1.
AP009048 Genomic DNA. Translation: BAE76628.1.
PIRiF64983.
RefSeqiNP_416656.1. NC_000913.3.
WP_000628648.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75212; AAC75212; b2151.
BAE76628; BAE76628; BAE76628.
GeneIDi949043.
KEGGiecj:JW2138.
eco:b2151.
PATRICi32119647. VBIEscCol129921_2234.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62529 Genomic DNA. Translation: CAA44390.1.
U00007 Genomic DNA. Translation: AAA60528.1.
U00096 Genomic DNA. Translation: AAC75212.1.
AP009048 Genomic DNA. Translation: BAE76628.1.
PIRiF64983.
RefSeqiNP_416656.1. NC_000913.3.
WP_000628648.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP25748.
SMRiP25748. Positions 2-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9734N.
IntActiP25748. 7 interactions.
STRINGi511145.b2151.

Proteomic databases

PaxDbiP25748.
PRIDEiP25748.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75212; AAC75212; b2151.
BAE76628; BAE76628; BAE76628.
GeneIDi949043.
KEGGiecj:JW2138.
eco:b2151.
PATRICi32119647. VBIEscCol129921_2234.

Organism-specific databases

EchoBASEiEB0360.
EcoGeneiEG10365. galS.

Phylogenomic databases

eggNOGiENOG41060NJ. Bacteria.
COG1609. LUCA.
HOGENOMiHOG000220179.
InParanoidiP25748.
KOiK02529.
OMAiTGSPDMQ.
OrthoDBiEOG6SJJMM.
PhylomeDBiP25748.

Enzyme and pathway databases

BioCyciEcoCyc:PD00261.
ECOL316407:JW2138-MONOMER.

Miscellaneous databases

PROiP25748.

Family and domain databases

Gene3Di1.10.260.40. 1 hit.
InterProiIPR000843. HTH_LacI.
IPR010982. Lambda_DNA-bd_dom.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00356. LacI. 1 hit.
[Graphical view]
PRINTSiPR00036. HTHLACI.
SMARTiSM00354. HTH_LACI. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF53822. SSF53822. 1 hit.
PROSITEiPS00356. HTH_LACI_1. 1 hit.
PS50932. HTH_LACI_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isorepressor of the gal regulon in Escherichia coli."
    Weickert M.J., Adhya S.
    J. Mol. Biol. 226:69-83(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / C600 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222.
  2. "Automated multiplex sequencing of the E.coli genome."
    Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / BHB2600.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Cited for: DNA-BINDING.
  6. "Functional characterization of roles of GalR and GalS as regulators of the gal regulon."
    Geanacopoulos M., Adhya S.
    J. Bacteriol. 179:228-234(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-20, CHARACTERIZATION.

Entry informationi

Entry nameiGALS_ECOLI
AccessioniPrimary (citable) accession number: P25748
Secondary accession number(s): Q2MAS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.