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P25746

- HFLD_ECOLI

UniProt

P25746 - HFLD_ECOLI

Protein

High frequency lysogenization protein HflD

Gene

hflD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Negative regulator of phage lambda lysogenization. Contributes to the degradation of the phage regulatory protein CII. Acts probably by holding CII on the membrane surface, away from the target promoters, but close to the FtsH protease.1 Publication

    GO - Molecular functioni

    1. protein binding Source: EcoCyc

    GO - Biological processi

    1. response to heat Source: EcoliWiki

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11345-MONOMER.
    ECOL316407:JW5165-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High frequency lysogenization protein HflD
    Gene namesi
    Name:hflD
    Synonyms:ycfC
    Ordered Locus Names:b1132, JW5165
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11345. hflD.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extrinsic component of cytoplasmic side of plasma membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene show an increased lysogenization frequency.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 213213High frequency lysogenization protein HflDPRO_0000071576Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP25746.

    Interactioni

    Subunit structurei

    Interacts with CII protein from phage lambda.1 Publication

    Protein-protein interaction databases

    STRINGi511145.b1132.

    Structurei

    Secondary structure

    1
    213
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 2824
    Helixi33 – 4412
    Helixi51 – 544
    Helixi59 – 624
    Helixi63 – 7412
    Beta strandi78 – 803
    Helixi81 – 10121
    Helixi105 – 11410
    Helixi117 – 1204
    Helixi128 – 14114
    Helixi143 – 1453
    Helixi155 – 1584
    Helixi161 – 18323
    Helixi188 – 20821

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1QZ4X-ray2.00A2-213[»]
    1SDIX-ray1.65A2-213[»]
    ProteinModelPortaliP25746.
    SMRiP25746. Positions 2-213.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP25746.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili79 – 12648Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HflD family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG2915.
    HOGENOMiHOG000119128.
    KOiK07153.
    OMAiDRQLAHY.
    OrthoDBiEOG6J1DBP.

    Family and domain databases

    Gene3Di1.10.3890.10. 1 hit.
    HAMAPiMF_00695. HflD_protein.
    InterProiIPR007451. UPF0274.
    [Graphical view]
    PfamiPF04356. DUF489. 1 hit.
    [Graphical view]
    ProDomiPD037115. UPF0274. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF101322. SSF101322. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P25746-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKNYYDITL ALAGICQSAR LVQQLAHQGH CDADALHVSL NSIIDMNPSS    50
    TLAVFGGSEA NLRVGLETLL GVLNASSRQG LNAELTRYTL SLMVLERKLS 100
    SAKGALDTLG NRINGLQRQL EHFDLQSETL MSAMAAIYVD VISPLGPRIQ 150
    VTGSPAVLQS PQVQAKVRAT LLAGIRAAVL WHQVGGGRLQ LMFSRNRLTT 200
    QAKQILAHLT PEL 213
    Length:213
    Mass (Da):22,948
    Last modified:May 1, 1992 - v1
    Checksum:iE26EF9698C3CEE42
    GO

    Sequence cautioni

    The sequence M74924 differs from that shown. Reason: Frameshift at positions 49, 174 and 178.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59307 Genomic DNA. Translation: CAA41995.1.
    M74924 Genomic DNA. No translation available.
    U00096 Genomic DNA. Translation: AAC74216.1.
    AP009048 Genomic DNA. Translation: BAA35954.1.
    PIRiS19211.
    RefSeqiNP_415650.1. NC_000913.3.
    YP_489400.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74216; AAC74216; b1132.
    BAA35954; BAA35954; BAA35954.
    GeneIDi12934136.
    945693.
    KEGGiecj:Y75_p1102.
    eco:b1132.
    PATRICi32117513. VBIEscCol129921_1179.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59307 Genomic DNA. Translation: CAA41995.1 .
    M74924 Genomic DNA. No translation available.
    U00096 Genomic DNA. Translation: AAC74216.1 .
    AP009048 Genomic DNA. Translation: BAA35954.1 .
    PIRi S19211.
    RefSeqi NP_415650.1. NC_000913.3.
    YP_489400.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1QZ4 X-ray 2.00 A 2-213 [» ]
    1SDI X-ray 1.65 A 2-213 [» ]
    ProteinModelPortali P25746.
    SMRi P25746. Positions 2-213.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b1132.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74216 ; AAC74216 ; b1132 .
    BAA35954 ; BAA35954 ; BAA35954 .
    GeneIDi 12934136.
    945693.
    KEGGi ecj:Y75_p1102.
    eco:b1132.
    PATRICi 32117513. VBIEscCol129921_1179.

    Organism-specific databases

    EchoBASEi EB1321.
    EcoGenei EG11345. hflD.

    Phylogenomic databases

    eggNOGi COG2915.
    HOGENOMi HOG000119128.
    KOi K07153.
    OMAi DRQLAHY.
    OrthoDBi EOG6J1DBP.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11345-MONOMER.
    ECOL316407:JW5165-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P25746.
    PROi P25746.

    Gene expression databases

    Genevestigatori P25746.

    Family and domain databases

    Gene3Di 1.10.3890.10. 1 hit.
    HAMAPi MF_00695. HflD_protein.
    InterProi IPR007451. UPF0274.
    [Graphical view ]
    Pfami PF04356. DUF489. 1 hit.
    [Graphical view ]
    ProDomi PD037115. UPF0274. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF101322. SSF101322. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The purB gene of Escherichia coli K-12 is located in an operon."
      Green S.M., Malik T., Giles I.G., Drabble W.T.
      Microbiology 142:3219-3230(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR."
      He B., Smith J.M., Zalkin H.
      J. Bacteriol. 174:130-136(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Revisiting the lysogenization control of bacteriophage lambda. Identification and characterization of a new host component, HflD."
      Kihara A., Akiyama Y., Ito K.
      J. Biol. Chem. 276:13695-13700(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CII, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    7. "Structure of a hypothetical protein ycfC coded by Escherichia coli genome."
      Midwest center for structural genomics (MCSG)
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 2-213.

    Entry informationi

    Entry nameiHFLD_ECOLI
    AccessioniPrimary (citable) accession number: P25746
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

    External Data

    Dasty 3