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Protein

High frequency lysogenization protein HflD

Gene

hflD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negative regulator of phage lambda lysogenization. Contributes to the degradation of the phage regulatory protein CII. Acts probably by holding CII on the membrane surface, away from the target promoters, but close to the FtsH protease.1 Publication

GO - Biological processi

  • response to heat Source: EcoliWiki

Enzyme and pathway databases

BioCyciEcoCyc:EG11345-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
High frequency lysogenization protein HflD
Gene namesi
Name:hflD
Synonyms:ycfC
Ordered Locus Names:b1132, JW5165
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11345 hflD

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extrinsic component of cytoplasmic side of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show an increased lysogenization frequency.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000715761 – 213High frequency lysogenization protein HflDAdd BLAST213

Proteomic databases

PaxDbiP25746
PRIDEiP25746

Interactioni

Subunit structurei

Interacts with CII protein from phage lambda.1 Publication

Protein-protein interaction databases

BioGridi4263130, 20 interactors
STRINGi316385.ECDH10B_1204

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 28Combined sources24
Helixi33 – 44Combined sources12
Helixi51 – 54Combined sources4
Helixi59 – 62Combined sources4
Helixi63 – 74Combined sources12
Beta strandi78 – 80Combined sources3
Helixi81 – 101Combined sources21
Helixi105 – 114Combined sources10
Helixi117 – 120Combined sources4
Helixi128 – 141Combined sources14
Helixi143 – 145Combined sources3
Helixi155 – 158Combined sources4
Helixi161 – 183Combined sources23
Helixi188 – 208Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QZ4X-ray2.00A2-213[»]
1SDIX-ray1.65A2-213[»]
ProteinModelPortaliP25746
SMRiP25746
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25746

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili79 – 126Sequence analysisAdd BLAST48

Sequence similaritiesi

Belongs to the HflD family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG41075W9 Bacteria
COG2915 LUCA
HOGENOMiHOG000119128
InParanoidiP25746
KOiK07153
OMAiRSAVLWH

Family and domain databases

Gene3Di1.10.3890.10, 1 hit
HAMAPiMF_00695 HflD_protein, 1 hit
InterProiView protein in InterPro
IPR007451 HflD
IPR035932 HflD-like_sf
PANTHERiPTHR38100 PTHR38100, 1 hit
PfamiView protein in Pfam
PF04356 DUF489, 1 hit
SUPFAMiSSF101322 SSF101322, 1 hit

Sequencei

Sequence statusi: Complete.

P25746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNYYDITL ALAGICQSAR LVQQLAHQGH CDADALHVSL NSIIDMNPSS
60 70 80 90 100
TLAVFGGSEA NLRVGLETLL GVLNASSRQG LNAELTRYTL SLMVLERKLS
110 120 130 140 150
SAKGALDTLG NRINGLQRQL EHFDLQSETL MSAMAAIYVD VISPLGPRIQ
160 170 180 190 200
VTGSPAVLQS PQVQAKVRAT LLAGIRAAVL WHQVGGGRLQ LMFSRNRLTT
210
QAKQILAHLT PEL
Length:213
Mass (Da):22,948
Last modified:May 1, 1992 - v1
Checksum:iE26EF9698C3CEE42
GO

Sequence cautioni

The sequence M74924 differs from that shown. Reason: Frameshift at positions 49, 174 and 178.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59307 Genomic DNA Translation: CAA41995.1
M74924 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAC74216.1
AP009048 Genomic DNA Translation: BAA35954.1
PIRiS19211
RefSeqiNP_415650.1, NC_000913.3
WP_001297479.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74216; AAC74216; b1132
BAA35954; BAA35954; BAA35954
GeneIDi945693
KEGGiecj:JW5165
eco:b1132
PATRICifig|1411691.4.peg.1134

Similar proteinsi

Entry informationi

Entry nameiHFLD_ECOLI
AccessioniPrimary (citable) accession number: P25746
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: March 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
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Main funding by: National Institutes of Health