P25746 (HFLD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High frequency lysogenization protein HflD

Gene names

Name:	hflD
Synonyms:	ycfC
Ordered Locus Names:	b1132, JW5165

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negative regulator of phage lambda lysogenization. Contributes to the degradation of the phage regulatory protein CII. Acts probably by holding CII on the membrane surface, away from the target promoters, but close to the FtsH protease. Ref.6
Subunit structure	Interacts with CII protein from phage lambda. Ref.6
Subcellular location	Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side Ref.6.
Disruption phenotype	Cells lacking this gene show an increased lysogenization frequency. Ref.6
Sequence similarities	Belongs to the HflD family.
Sequence caution	The sequence M74924 differs from that shown. Reason: Frameshift at positions 49, 174 and 178.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Cytoplasm Membrane
Domain	Coiled coil
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to heat Inferred from expression pattern PubMed 8349564. Source: EcoliWiki
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to internal side of plasma membrane Inferred from direct assay Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 213

213

High frequency lysogenization protein HflD HAMAP-Rule MF_00695

PRO_0000071576

Regions

Coiled coil

79 – 126

48

Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

213

Helix Strand Turn

Details...

Helix

5 – 28

24

Helix

33 – 44

12

Helix

51 – 54

4

Helix

59 – 62

4

Helix

63 – 74

12

Beta strand

78 – 80

3

Helix

81 – 101

21

Helix

105 – 114

10

Helix

117 – 120

4

Helix

128 – 141

14

Helix

143 – 145

3

Helix

155 – 158

4

Helix

161 – 183

23

Helix

188 – 208

21

Sequences

	Sequence		Length	Mass (Da)
	P25746 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: E26EF9698C3CEE42	FASTA	213	22,948
	10 20 30 40 50 60 MAKNYYDITL ALAGICQSAR LVQQLAHQGH CDADALHVSL NSIIDMNPSS TLAVFGGSEA 70 80 90 100 110 120 NLRVGLETLL GVLNASSRQG LNAELTRYTL SLMVLERKLS SAKGALDTLG NRINGLQRQL 130 140 150 160 170 180 EHFDLQSETL MSAMAAIYVD VISPLGPRIQ VTGSPAVLQS PQVQAKVRAT LLAGIRAAVL 190 200 210 WHQVGGGRLQ LMFSRNRLTT QAKQILAHLT PEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The purB gene of Escherichia coli K-12 is located in an operon." Green S.M., Malik T., Giles I.G., Drabble W.T. Microbiology 142:3219-3230(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR." He B., Smith J.M., Zalkin H. J. Bacteriol. 174:130-136(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Revisiting the lysogenization control of bacteriophage lambda. Identification and characterization of a new host component, HflD." Kihara A., Akiyama Y., Ito K. J. Biol. Chem. 276:13695-13700(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CII, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[7]	"Structure of a hypothetical protein ycfC coded by Escherichia coli genome." Midwest center for structural genomics (MCSG) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 2-213.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X59307 Genomic DNA. Translation: CAA41995.1.
M74924 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74216.1.
AP009048 Genomic DNA. Translation: BAA35954.1.

PIR

S19211.

RefSeq

NP_415650.1. NC_000913.2.
YP_489400.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QZ4	X-ray	2.00	A	2-213	[»]
1SDI	X-ray	1.65	A	2-213	[»]

ProteinModelPortal

P25746.

SMR

P25746. Positions 2-213.

ModBase

Search...

Protein-protein interaction databases

STRING

511145.b1132.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74216; AAC74216; b1132.
BAA35954; BAA35954; BAA35954.

GeneID

12934136.
945693.

KEGG

ecj:Y75_p1102.
eco:b1132.

PATRIC

32117513. VBIEscCol129921_1179.

Organism-specific databases

EchoBASE

EB1321.

EcoGene

EG11345. hflD.

Phylogenomic databases

eggNOG

COG2915.

HOGENOM

HOG000119128.

KO

K07153.

OMA

VRTAMDS.

ProtClustDB

PRK00218.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11345-MONOMER.
ECOL316407:JW5165-MONOMER.

Gene expression databases

Genevestigator

P25746.

Family and domain databases

HAMAP

MF_00695. HflD_protein.

InterPro

IPR007451. UPF0274.
[Graphical view]

Pfam

PF04356. DUF489. 1 hit.
[Graphical view]

ProDom

PD037115. UPF0274. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF101322. DUF489. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P25746.

Entry information

Entry name

HFLD_ECOLI

Accession

Primary (citable) accession number: P25746

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program