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Protein

High frequency lysogenization protein HflD

Gene

hflD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of phage lambda lysogenization. Contributes to the degradation of the phage regulatory protein CII. Acts probably by holding CII on the membrane surface, away from the target promoters, but close to the FtsH protease.1 Publication

GO - Biological processi

  • response to heat Source: EcoliWiki
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG11345-MONOMER.
ECOL316407:JW5165-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
High frequency lysogenization protein HflD
Gene namesi
Name:hflD
Synonyms:ycfC
Ordered Locus Names:b1132, JW5165
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11345. hflD.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extrinsic component of cytoplasmic side of plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show an increased lysogenization frequency.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213High frequency lysogenization protein HflDPRO_0000071576Add
BLAST

Interactioni

Subunit structurei

Interacts with CII protein from phage lambda.1 Publication

Protein-protein interaction databases

STRINGi511145.b1132.

Structurei

Secondary structure

1
213
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2824Combined sources
Helixi33 – 4412Combined sources
Helixi51 – 544Combined sources
Helixi59 – 624Combined sources
Helixi63 – 7412Combined sources
Beta strandi78 – 803Combined sources
Helixi81 – 10121Combined sources
Helixi105 – 11410Combined sources
Helixi117 – 1204Combined sources
Helixi128 – 14114Combined sources
Helixi143 – 1453Combined sources
Helixi155 – 1584Combined sources
Helixi161 – 18323Combined sources
Helixi188 – 20821Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QZ4X-ray2.00A2-213[»]
1SDIX-ray1.65A2-213[»]
ProteinModelPortaliP25746.
SMRiP25746. Positions 2-213.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25746.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili79 – 12648Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the HflD family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG2915.
HOGENOMiHOG000119128.
InParanoidiP25746.
KOiK07153.
OMAiRSAVLWH.
OrthoDBiEOG6J1DBP.

Family and domain databases

Gene3Di1.10.3890.10. 1 hit.
HAMAPiMF_00695. HflD_protein.
InterProiIPR007451. UPF0274.
[Graphical view]
PfamiPF04356. DUF489. 1 hit.
[Graphical view]
ProDomiPD037115. UPF0274. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF101322. SSF101322. 1 hit.

Sequencei

Sequence statusi: Complete.

P25746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNYYDITL ALAGICQSAR LVQQLAHQGH CDADALHVSL NSIIDMNPSS
60 70 80 90 100
TLAVFGGSEA NLRVGLETLL GVLNASSRQG LNAELTRYTL SLMVLERKLS
110 120 130 140 150
SAKGALDTLG NRINGLQRQL EHFDLQSETL MSAMAAIYVD VISPLGPRIQ
160 170 180 190 200
VTGSPAVLQS PQVQAKVRAT LLAGIRAAVL WHQVGGGRLQ LMFSRNRLTT
210
QAKQILAHLT PEL
Length:213
Mass (Da):22,948
Last modified:May 1, 1992 - v1
Checksum:iE26EF9698C3CEE42
GO

Sequence cautioni

The sequence M74924 differs from that shown. Reason: Frameshift at positions 49, 174 and 178. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59307 Genomic DNA. Translation: CAA41995.1.
M74924 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74216.1.
AP009048 Genomic DNA. Translation: BAA35954.1.
PIRiS19211.
RefSeqiNP_415650.1. NC_000913.3.
WP_001297479.1. NZ_CP010445.1.

Genome annotation databases

EnsemblBacteriaiAAC74216; AAC74216; b1132.
BAA35954; BAA35954; BAA35954.
GeneIDi945693.
KEGGieco:b1132.
PATRICi32117513. VBIEscCol129921_1179.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59307 Genomic DNA. Translation: CAA41995.1.
M74924 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74216.1.
AP009048 Genomic DNA. Translation: BAA35954.1.
PIRiS19211.
RefSeqiNP_415650.1. NC_000913.3.
WP_001297479.1. NZ_CP010445.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QZ4X-ray2.00A2-213[»]
1SDIX-ray1.65A2-213[»]
ProteinModelPortaliP25746.
SMRiP25746. Positions 2-213.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b1132.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74216; AAC74216; b1132.
BAA35954; BAA35954; BAA35954.
GeneIDi945693.
KEGGieco:b1132.
PATRICi32117513. VBIEscCol129921_1179.

Organism-specific databases

EchoBASEiEB1321.
EcoGeneiEG11345. hflD.

Phylogenomic databases

eggNOGiCOG2915.
HOGENOMiHOG000119128.
InParanoidiP25746.
KOiK07153.
OMAiRSAVLWH.
OrthoDBiEOG6J1DBP.

Enzyme and pathway databases

BioCyciEcoCyc:EG11345-MONOMER.
ECOL316407:JW5165-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP25746.
PROiP25746.

Family and domain databases

Gene3Di1.10.3890.10. 1 hit.
HAMAPiMF_00695. HflD_protein.
InterProiIPR007451. UPF0274.
[Graphical view]
PfamiPF04356. DUF489. 1 hit.
[Graphical view]
ProDomiPD037115. UPF0274. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF101322. SSF101322. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The purB gene of Escherichia coli K-12 is located in an operon."
    Green S.M., Malik T., Giles I.G., Drabble W.T.
    Microbiology 142:3219-3230(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR."
    He B., Smith J.M., Zalkin H.
    J. Bacteriol. 174:130-136(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Revisiting the lysogenization control of bacteriophage lambda. Identification and characterization of a new host component, HflD."
    Kihara A., Akiyama Y., Ito K.
    J. Biol. Chem. 276:13695-13700(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CII, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  7. "Structure of a hypothetical protein ycfC coded by Escherichia coli genome."
    Midwest center for structural genomics (MCSG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 2-213.

Entry informationi

Entry nameiHFLD_ECOLI
AccessioniPrimary (citable) accession number: P25746
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: July 22, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.