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Protein

Lipopolysaccharide core heptosyltransferase RfaQ

Gene

rfaQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core (By similarity).By similarity

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

  • lipopolysaccharide core region biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11341-MONOMER.
ECOL316407:JW3607-MONOMER.
MetaCyc:EG11341-MONOMER.
UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT9. Glycosyltransferase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide core heptosyltransferase RfaQ (EC:2.-.-.-)
Gene namesi
Name:rfaQ
Synonyms:waaQ
Ordered Locus Names:b3632, JW3607
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11341. rfaQ.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Lipopolysaccharide core heptosyltransferase RfaQPRO_0000207265Add
BLAST

Proteomic databases

PaxDbiP25742.
PRIDEiP25742.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F51EBI-555525,EBI-543750

Protein-protein interaction databases

BioGridi4259595. 220 interactions.
DIPiDIP-10675N.
IntActiP25742. 2 interactions.
MINTiMINT-1275410.
STRINGi511145.b3632.

Structurei

3D structure databases

ProteinModelPortaliP25742.
SMRiP25742. Positions 5-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 9 family.Curated

Phylogenomic databases

eggNOGiENOG4105DKP. Bacteria.
COG0859. LUCA.
HOGENOMiHOG000010798.
InParanoidiP25742.
KOiK02849.
OMAiSMPMHLA.
OrthoDBiEOG6DRPDK.
PhylomeDBiP25742.

Family and domain databases

InterProiIPR002201. Glyco_trans_9.
IPR011916. LipoPS_heptosylTferase-III.
[Graphical view]
PfamiPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02201. heptsyl_trn_III. 1 hit.

Sequencei

Sequence statusi: Complete.

P25742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYHGDMLLT TPVISTLKQN YPDAKIDMLL YQDTIPILSE NPEINALYGI
60 70 80 90 100
SNKGAGTFDK IKNVLSLIKT LRANNYDLVI NLTDQWMVAL LVRCLPARMK
110 120 130 140 150
ISQLYGHRQH GIWKKSFTHL APIHGTHIVE RNLSVLEPLG ITDFYTDTTM
160 170 180 190 200
SYAEDCWKKM RRELDALGVK DHYVVIQPTA RQIFKCWDND KFSKVIDALQ
210 220 230 240 250
QRGYQVVLTC GPSADDLACV DEIARGCETK PITGLAGKTR FPELGALIDH
260 270 280 290 300
AVLFIGVDSA PGHIAAAVKT PVISLFGATD HVFWRPWTEN IIQFWAGNYQ
310 320 330 340
KMPTRHELDR NKKYLSVIPA EDVIAATEKL LPEDAPSADR NAQL
Length:344
Mass (Da):38,731
Last modified:June 1, 1994 - v2
Checksum:i1C788368BE46404A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80599 Genomic DNA. Translation: AAA24081.1.
M86305 Genomic DNA. Translation: AAA03744.1.
U00039 Genomic DNA. Translation: AAB18609.1.
U00096 Genomic DNA. Translation: AAC76656.1.
AP009048 Genomic DNA. Translation: BAE77660.1.
PIRiS27560.
RefSeqiNP_418089.1. NC_000913.3.
WP_001268851.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76656; AAC76656; b3632.
BAE77660; BAE77660; BAE77660.
GeneIDi948155.
KEGGiecj:JW3607.
eco:b3632.
PATRICi32122751. VBIEscCol129921_3752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80599 Genomic DNA. Translation: AAA24081.1.
M86305 Genomic DNA. Translation: AAA03744.1.
U00039 Genomic DNA. Translation: AAB18609.1.
U00096 Genomic DNA. Translation: AAC76656.1.
AP009048 Genomic DNA. Translation: BAE77660.1.
PIRiS27560.
RefSeqiNP_418089.1. NC_000913.3.
WP_001268851.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP25742.
SMRiP25742. Positions 5-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259595. 220 interactions.
DIPiDIP-10675N.
IntActiP25742. 2 interactions.
MINTiMINT-1275410.
STRINGi511145.b3632.

Protein family/group databases

CAZyiGT9. Glycosyltransferase Family 9.

Proteomic databases

PaxDbiP25742.
PRIDEiP25742.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76656; AAC76656; b3632.
BAE77660; BAE77660; BAE77660.
GeneIDi948155.
KEGGiecj:JW3607.
eco:b3632.
PATRICi32122751. VBIEscCol129921_3752.

Organism-specific databases

EchoBASEiEB1317.
EcoGeneiEG11341. rfaQ.

Phylogenomic databases

eggNOGiENOG4105DKP. Bacteria.
COG0859. LUCA.
HOGENOMiHOG000010798.
InParanoidiP25742.
KOiK02849.
OMAiSMPMHLA.
OrthoDBiEOG6DRPDK.
PhylomeDBiP25742.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciEcoCyc:EG11341-MONOMER.
ECOL316407:JW3607-MONOMER.
MetaCyc:EG11341-MONOMER.

Miscellaneous databases

PROiP25742.

Family and domain databases

InterProiIPR002201. Glyco_trans_9.
IPR011916. LipoPS_heptosylTferase-III.
[Graphical view]
PfamiPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02201. heptsyl_trn_III. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12."
    Parker C.T., Pradel E., Schnaitman C.A.
    J. Bacteriol. 174:930-934(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "The gene coding for 3-deoxy-manno-octulosonic acid transferase and the rfaQ gene are transcribed from divergently arranged promoters in Escherichia coli."
    Clementz T.
    J. Bacteriol. 174:7750-7756(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiRFAQ_ECOLI
AccessioniPrimary (citable) accession number: P25742
Secondary accession number(s): Q2M7U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 1, 1994
Last modified: March 16, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.