P25741 (RFAP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipopolysaccharide core heptose(I) kinase RfaP
EC=2.7.1.-

Gene names

Name:	rfaP
Synonyms:	waaP
Ordered Locus Names:	b3630, JW3605

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core By similarity.
Cofactor	Magnesium By similarity.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the protein kinase superfamily. KdkA/RfaP family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipopolysaccharide core region biosynthetic process Inferred from genetic interaction PubMed 1348243. Source: EcoCyc
Cellular_component	membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from direct assay PubMed 11069912. Source: EcoCyc phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Lipopolysaccharide core heptose(I) kinase RfaP		PRO_0000097285
Sites
	Active site	162	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P25741 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: E77F1E9956ABDBED	FASTA	265	30,872
	10 20 30 40 50 60 MVELKEPLAT LWRGKDAFAE VKKLNGEVFR ELETRRTLRF ELSGKSYFLK WHKGTTLKEI 70 80 90 100 110 120 IKNLLSLRMP VLGADREWHA IHRLSDVGVD TMKGIGFGEK GLNPLTRASF IITEDLTPTI 130 140 150 160 170 180 SLEDYCADWA VNPPDIRVKR MLIARVATMV RKMHTAGINH RDCYICHFLL HLPFTGREDE 190 200 210 220 230 240 LKISVIDLHR AQIRAKVPRR WRDKDLIGLY FSSMNIGLTQ RDIWRFMKVY FGMPLRKILS 250 260 LEQNLLNMAS VKAERIKERT QRKGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12." Parker C.T., Pradel E., Schnaitman C.A. J. Bacteriol. 174:930-934(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M80599 Genomic DNA. Translation: AAA24083.1. U00039 Genomic DNA. Translation: AAB18607.1. U00096 Genomic DNA. Translation: AAC76654.1. AP009048 Genomic DNA. Translation: BAE77662.1.
PIR	C42595.
RefSeq	NP_418087.1. NC_000913.2. YP_491803.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P25741.
ModBase	Search...
Protein-protein interaction databases
IntAct	P25741. 7 interactions.
STRING	511145.b3630.
Proteomic databases
PaxDb	P25741.
PRIDE	P25741.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76654; AAC76654; b3630. BAE77662; BAE77662; BAE77662.
GeneID	12931810. 948150.
KEGG	ecj:Y75_p3544. eco:b3630.
PATRIC	32122747. VBIEscCol129921_3750.
Organism-specific databases
EchoBASE	EB1316.
EcoGene	EG11340. rfaP.
Phylogenomic databases
eggNOG	NOG04355.
HOGENOM	HOG000126215.
KO	K02848.
OMA	GVPTMTA.
ProtClustDB	PRK15123.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11340-MONOMER. ECOL316407:JW3605-MONOMER. MetaCyc:EG11340-MONOMER.
UniPathway	UPA00958.
Gene expression databases
Genevestigator	P25741.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR010440. LipoPS_kinase. IPR017172. Lsacc_core_hep_kinase_RfaP. [Graphical view]
Pfam	PF06293. Kdo. 1 hit. [Graphical view]
PIRSF	PIRSF037318. RfaP. 1 hit.
SUPFAM	SSF56112. Kinase_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFAP_ECOLI

Accession

Primary (citable) accession number: P25741
Secondary accession number(s): Q2M7U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program