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Protein

Lipopolysaccharide core heptose(I) kinase RfaP

Gene

rfaP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core.By similarity

Cofactori

Mg2+By similarity

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei162 – 1621Sequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11340-MONOMER.
ECOL316407:JW3605-MONOMER.
MetaCyc:EG11340-MONOMER.
UniPathwayiUPA00958.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide core heptose(I) kinase RfaP (EC:2.7.1.-)
Gene namesi
Name:rfaP
Synonyms:waaP
Ordered Locus Names:b3630, JW3605
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11340. rfaP.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265Lipopolysaccharide core heptose(I) kinase RfaPPRO_0000097285Add
BLAST

Proteomic databases

PaxDbiP25741.
PRIDEiP25741.

Interactioni

Protein-protein interaction databases

BioGridi4260804. 370 interactions.
IntActiP25741. 7 interactions.
STRINGi511145.b3630.

Structurei

3D structure databases

ProteinModelPortaliP25741.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107543. Bacteria.
ENOG410XQAT. LUCA.
HOGENOMiHOG000126215.
InParanoidiP25741.
KOiK02848.
OMAiGVPTMTA.
OrthoDBiEOG63JR6W.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR017172. Lsacc_core_hep_kinase_RfaP.
[Graphical view]
PfamiPF06293. Kdo. 1 hit.
[Graphical view]
PIRSFiPIRSF037318. RfaP. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

P25741-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVELKEPLAT LWRGKDAFAE VKKLNGEVFR ELETRRTLRF ELSGKSYFLK
60 70 80 90 100
WHKGTTLKEI IKNLLSLRMP VLGADREWHA IHRLSDVGVD TMKGIGFGEK
110 120 130 140 150
GLNPLTRASF IITEDLTPTI SLEDYCADWA VNPPDIRVKR MLIARVATMV
160 170 180 190 200
RKMHTAGINH RDCYICHFLL HLPFTGREDE LKISVIDLHR AQIRAKVPRR
210 220 230 240 250
WRDKDLIGLY FSSMNIGLTQ RDIWRFMKVY FGMPLRKILS LEQNLLNMAS
260
VKAERIKERT QRKGL
Length:265
Mass (Da):30,872
Last modified:May 1, 1992 - v1
Checksum:iE77F1E9956ABDBED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80599 Genomic DNA. Translation: AAA24083.1.
U00039 Genomic DNA. Translation: AAB18607.1.
U00096 Genomic DNA. Translation: AAC76654.1.
AP009048 Genomic DNA. Translation: BAE77662.1.
PIRiC42595.
RefSeqiNP_418087.1. NC_000913.3.
WP_000229840.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76654; AAC76654; b3630.
BAE77662; BAE77662; BAE77662.
GeneIDi948150.
KEGGiecj:JW3605.
eco:b3630.
PATRICi32122747. VBIEscCol129921_3750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80599 Genomic DNA. Translation: AAA24083.1.
U00039 Genomic DNA. Translation: AAB18607.1.
U00096 Genomic DNA. Translation: AAC76654.1.
AP009048 Genomic DNA. Translation: BAE77662.1.
PIRiC42595.
RefSeqiNP_418087.1. NC_000913.3.
WP_000229840.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP25741.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260804. 370 interactions.
IntActiP25741. 7 interactions.
STRINGi511145.b3630.

Proteomic databases

PaxDbiP25741.
PRIDEiP25741.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76654; AAC76654; b3630.
BAE77662; BAE77662; BAE77662.
GeneIDi948150.
KEGGiecj:JW3605.
eco:b3630.
PATRICi32122747. VBIEscCol129921_3750.

Organism-specific databases

EchoBASEiEB1316.
EcoGeneiEG11340. rfaP.

Phylogenomic databases

eggNOGiENOG4107543. Bacteria.
ENOG410XQAT. LUCA.
HOGENOMiHOG000126215.
InParanoidiP25741.
KOiK02848.
OMAiGVPTMTA.
OrthoDBiEOG63JR6W.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciEcoCyc:EG11340-MONOMER.
ECOL316407:JW3605-MONOMER.
MetaCyc:EG11340-MONOMER.

Miscellaneous databases

PROiP25741.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR017172. Lsacc_core_hep_kinase_RfaP.
[Graphical view]
PfamiPF06293. Kdo. 1 hit.
[Graphical view]
PIRSFiPIRSF037318. RfaP. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12."
    Parker C.T., Pradel E., Schnaitman C.A.
    J. Bacteriol. 174:930-934(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiRFAP_ECOLI
AccessioniPrimary (citable) accession number: P25741
Secondary accession number(s): Q2M7U4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: January 20, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.