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Reviewed, UniProtKB/Swiss-Prot P25740 (RFAG_ECOLI)

Last modified June 16, 2009. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Lipopolysaccharide core biosynthesis protein rfaG
    EC=2.4.-.-
Alternative name(s):
    Glucosyltransferase I
Gene names
Name: rfaG
Synonyms: pcsA, waaG
Ordered Locus Names: b3631, JW3606
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Involved in the addition of the first glucose residue to the lipopolysaccharide core.

Pathway

Bacterial outer membrane biogenesis; LPS core biosynthesis.

Sequence similarities

Belongs to the glycosyltransferase 1 family.

Ontologies

Keywords
   Biological processLipopolysaccharide biosynthesis
   Molecular functionGlycosyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processlipopolysaccharide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functiontransferase activity, transferring glycosyl groups

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374Lipopolysaccharide core biosynthesis protein rfaG
PRO_0000080305

Secondary structure

.................................................................... 374
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25740-1 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 7F20AE577CBB80C2

FASTA37442,284
        10         20         30         40         50         60 
MIVAFCLYKY FPFGGLQRDF MRIASTVAAR GHHVRVYTQS WEGDCPKAFE LIQVPVKSHT 

        70         80         90        100        110        120 
NHGRNAEYYA WVQNHLKEHP ADRVVGFNKM PGLDVYFAAD VCYAEKVAQE KGFLYRLTSR 

       130        140        150        160        170        180 
YRHYAAFERA TFEQGKSTKL MMLTDKQIAD FQKHYQTEPE RFQILPPGIY PDRKYSEQIP 

       190        200        210        220        230        240 
NSREIYRQKN GIKEQQNLLL QVGSDFGRKG VDRSIEALAS LPESLRHNTL LFVVGQDKPR 

       250        260        270        280        290        300 
KFEALAEKLG VRSNVHFFSG RNDVSELMAA ADLLLHPAYQ EAAGIVLLEA ITAGLPVLTT 

       310        320        330        340        350        360 
AVCGYAHYIA DANCGTVIAE PFSQEQLNEV LRKALTQSPL RMAWAENARH YADTQDLYSL 

       370 
PEKAADIITG GLDG 

« Hide

References

« Hide 'large scale' references
[1]"Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12."
Parker C.T., Pradel E., Schnaitman C.A.
J. Bacteriol. 174:930-934(1992) [PubMed: 1732225] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The gene coding for 3-deoxy-manno-octulosonic acid transferase and the rfaQ gene are transcribed from divergently arranged promoters in Escherichia coli."
Clementz T.
J. Bacteriol. 174:7750-7756(1992) [PubMed: 1447141] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-217.
[6]"Identification by Tn10 transposon mutagenesis of host factors involved in the biosynthesis of K99 fimbriae of Escherichia coli: effect of LPS core mutations."
Pilipcinec E., Huisman T.T., Willemsen P.T., Appelmelk B.J., Graaf F.K., Oudega B.
FEMS Microbiol. Lett. 123:201-206(1994) [PubMed: 7988890] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58.
+Additional computationally mapped references.

Cross-references

Sequence databases

M80599 Genomic DNA. Translation: AAA24082.1.
M86305 Genomic DNA. Translation: AAA03743.2.
U00039 Genomic DNA. Translation: AAB18608.1.
U00096 Genomic DNA. Translation: AAC76655.1.
AP009048 Genomic DNA. Translation: BAE77661.1.
S75736 Genomic DNA. Translation: AAD43826.1.
PIRB42595.
RefSeqAP_004160.1.
NP_418088.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2IV7X-ray1.60A1-374[»]
2IW1X-ray1.50A1-374[»]
ModBaseSearch...

Protein family/group databases

CAZyGT4. Glycosyltransferase Family 4.

Genome annotation databases

GeneID948149.
GenomeReviewsGene locus JW3606 in contig AP009048_GR.
Gene locus b3631 in contig U00096_GR.
KEGGecj:JW3606.
eco:b3631.

Organism-specific databases

EchoBASEEB1315.
EcoGeneEG11339. rfaG.
CMRSearch...

Phylogenomic databases

HOGENOMP25740.
OMAP25740. LMISEVQ.

Enzyme and pathway databases

BioCycEcoCyc:EG11339-MON.
MetaCyc:EG11339-MON.

Family and domain databases

InterProIPR001296. Glyco_trans_1.
[Graphical view]
PfamPF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRFAG_ECOLI
AccessionPrimary (citable) accession number: P25740
Secondary accession number(s): Q2M7U5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents