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Protein

Lipopolysaccharide core biosynthesis protein RfaG

Gene

rfaG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the addition of the first glucose residue to the lipopolysaccharide core.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • lipopolysaccharide glucosyltransferase I activity Source: EcoCyc

GO - Biological processi

  • lipopolysaccharide core region biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11339-MONOMER.
ECOL316407:JW3606-MONOMER.
MetaCyc:EG11339-MONOMER.
BRENDAi2.4.1.B64. 2026.
UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide core biosynthesis protein RfaG (EC:2.4.-.-)
Alternative name(s):
Glucosyltransferase I
Gene namesi
Name:rfaG
Synonyms:pcsA, waaG
Ordered Locus Names:b3631, JW3606
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11339. rfaG.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000803051 – 374Lipopolysaccharide core biosynthesis protein RfaGAdd BLAST374

Proteomic databases

PaxDbiP25740.
PRIDEiP25740.

Interactioni

Protein-protein interaction databases

BioGridi4260869. 274 interactors.
IntActiP25740. 10 interactors.
STRINGi511145.b3631.

Structurei

Secondary structure

1374
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Beta strandi8 – 10Combined sources3
Helixi15 – 29Combined sources15
Beta strandi34 – 41Combined sources8
Beta strandi50 – 53Combined sources4
Helixi61 – 78Combined sources18
Beta strandi82 – 88Combined sources7
Beta strandi94 – 97Combined sources4
Helixi103 – 110Combined sources8
Helixi113 – 116Combined sources4
Helixi119 – 132Combined sources14
Beta strandi139 – 143Combined sources5
Helixi145 – 155Combined sources11
Helixi159 – 161Combined sources3
Beta strandi162 – 164Combined sources3
Helixi171 – 173Combined sources3
Helixi175 – 177Combined sources3
Helixi182 – 189Combined sources8
Beta strandi197 – 202Combined sources6
Turni206 – 210Combined sources5
Helixi211 – 219Combined sources9
Helixi223 – 227Combined sources5
Beta strandi229 – 237Combined sources9
Helixi240 – 248Combined sources9
Helixi252 – 254Combined sources3
Beta strandi255 – 259Combined sources5
Helixi264 – 270Combined sources7
Beta strandi272 – 276Combined sources5
Helixi285 – 293Combined sources9
Beta strandi297 – 300Combined sources4
Turni304 – 306Combined sources3
Helixi307 – 312Combined sources6
Beta strandi315 – 318Combined sources4
Helixi324 – 336Combined sources13
Helixi338 – 354Combined sources17
Helixi360 – 369Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IV7X-ray1.60A1-374[»]
2IW1X-ray1.50A1-374[»]
2N58NMR-A103-132[»]
ProteinModelPortaliP25740.
SMRiP25740.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25740.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107RJ7. Bacteria.
ENOG410XPWS. LUCA.
HOGENOMiHOG000126226.
InParanoidiP25740.
KOiK02844.
OMAiIHICNPR.
PhylomeDBiP25740.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVAFCLYKY FPFGGLQRDF MRIASTVAAR GHHVRVYTQS WEGDCPKAFE
60 70 80 90 100
LIQVPVKSHT NHGRNAEYYA WVQNHLKEHP ADRVVGFNKM PGLDVYFAAD
110 120 130 140 150
VCYAEKVAQE KGFLYRLTSR YRHYAAFERA TFEQGKSTKL MMLTDKQIAD
160 170 180 190 200
FQKHYQTEPE RFQILPPGIY PDRKYSEQIP NSREIYRQKN GIKEQQNLLL
210 220 230 240 250
QVGSDFGRKG VDRSIEALAS LPESLRHNTL LFVVGQDKPR KFEALAEKLG
260 270 280 290 300
VRSNVHFFSG RNDVSELMAA ADLLLHPAYQ EAAGIVLLEA ITAGLPVLTT
310 320 330 340 350
AVCGYAHYIA DANCGTVIAE PFSQEQLNEV LRKALTQSPL RMAWAENARH
360 370
YADTQDLYSL PEKAADIITG GLDG
Length:374
Mass (Da):42,284
Last modified:May 1, 1992 - v1
Checksum:i7F20AE577CBB80C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80599 Genomic DNA. Translation: AAA24082.1.
M86305 Genomic DNA. Translation: AAA03743.2.
U00039 Genomic DNA. Translation: AAB18608.1.
U00096 Genomic DNA. Translation: AAC76655.1.
AP009048 Genomic DNA. Translation: BAE77661.1.
S75736 Genomic DNA. Translation: AAD43826.1.
PIRiB42595.
RefSeqiNP_418088.1. NC_000913.3.
WP_000634283.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76655; AAC76655; b3631.
BAE77661; BAE77661; BAE77661.
GeneIDi948149.
KEGGiecj:JW3606.
eco:b3631.
PATRICi32122749. VBIEscCol129921_3751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80599 Genomic DNA. Translation: AAA24082.1.
M86305 Genomic DNA. Translation: AAA03743.2.
U00039 Genomic DNA. Translation: AAB18608.1.
U00096 Genomic DNA. Translation: AAC76655.1.
AP009048 Genomic DNA. Translation: BAE77661.1.
S75736 Genomic DNA. Translation: AAD43826.1.
PIRiB42595.
RefSeqiNP_418088.1. NC_000913.3.
WP_000634283.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IV7X-ray1.60A1-374[»]
2IW1X-ray1.50A1-374[»]
2N58NMR-A103-132[»]
ProteinModelPortaliP25740.
SMRiP25740.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260869. 274 interactors.
IntActiP25740. 10 interactors.
STRINGi511145.b3631.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

PaxDbiP25740.
PRIDEiP25740.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76655; AAC76655; b3631.
BAE77661; BAE77661; BAE77661.
GeneIDi948149.
KEGGiecj:JW3606.
eco:b3631.
PATRICi32122749. VBIEscCol129921_3751.

Organism-specific databases

EchoBASEiEB1315.
EcoGeneiEG11339. rfaG.

Phylogenomic databases

eggNOGiENOG4107RJ7. Bacteria.
ENOG410XPWS. LUCA.
HOGENOMiHOG000126226.
InParanoidiP25740.
KOiK02844.
OMAiIHICNPR.
PhylomeDBiP25740.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciEcoCyc:EG11339-MONOMER.
ECOL316407:JW3606-MONOMER.
MetaCyc:EG11339-MONOMER.
BRENDAi2.4.1.B64. 2026.

Miscellaneous databases

EvolutionaryTraceiP25740.
PROiP25740.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFAG_ECOLI
AccessioniPrimary (citable) accession number: P25740
Secondary accession number(s): Q2M7U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.