Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lipopolysaccharide core biosynthesis protein RfaG

Gene

rfaG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the addition of the first glucose residue to the lipopolysaccharide core.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

  • lipopolysaccharide glucosyltransferase I activity Source: EcoCyc

GO - Biological processi

  • lipopolysaccharide core region biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11339-MONOMER.
ECOL316407:JW3606-MONOMER.
MetaCyc:EG11339-MONOMER.
BRENDAi2.4.1.B64. 2026.
UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide core biosynthesis protein RfaG (EC:2.4.-.-)
Alternative name(s):
Glucosyltransferase I
Gene namesi
Name:rfaG
Synonyms:pcsA, waaG
Ordered Locus Names:b3631, JW3606
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11339. rfaG.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 374374Lipopolysaccharide core biosynthesis protein RfaGPRO_0000080305Add
BLAST

Proteomic databases

PaxDbiP25740.
PRIDEiP25740.

Interactioni

Protein-protein interaction databases

BioGridi4260869. 274 interactions.
IntActiP25740. 10 interactions.
STRINGi511145.b3631.

Structurei

Secondary structure

1
374
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 64Combined sources
Beta strandi8 – 103Combined sources
Helixi15 – 2915Combined sources
Beta strandi34 – 418Combined sources
Beta strandi50 – 534Combined sources
Helixi61 – 7818Combined sources
Beta strandi82 – 887Combined sources
Beta strandi94 – 974Combined sources
Helixi103 – 1108Combined sources
Helixi113 – 1164Combined sources
Helixi119 – 13214Combined sources
Beta strandi139 – 1435Combined sources
Helixi145 – 15511Combined sources
Helixi159 – 1613Combined sources
Beta strandi162 – 1643Combined sources
Helixi171 – 1733Combined sources
Helixi175 – 1773Combined sources
Helixi182 – 1898Combined sources
Beta strandi197 – 2026Combined sources
Turni206 – 2105Combined sources
Helixi211 – 2199Combined sources
Helixi223 – 2275Combined sources
Beta strandi229 – 2379Combined sources
Helixi240 – 2489Combined sources
Helixi252 – 2543Combined sources
Beta strandi255 – 2595Combined sources
Helixi264 – 2707Combined sources
Beta strandi272 – 2765Combined sources
Helixi285 – 2939Combined sources
Beta strandi297 – 3004Combined sources
Turni304 – 3063Combined sources
Helixi307 – 3126Combined sources
Beta strandi315 – 3184Combined sources
Helixi324 – 33613Combined sources
Helixi338 – 35417Combined sources
Helixi360 – 36910Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IV7X-ray1.60A1-374[»]
2IW1X-ray1.50A1-374[»]
2N58NMR-A103-132[»]
ProteinModelPortaliP25740.
SMRiP25740. Positions 2-371.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25740.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107RJ7. Bacteria.
ENOG410XPWS. LUCA.
HOGENOMiHOG000126226.
InParanoidiP25740.
KOiK02844.
OMAiIHICNPR.
PhylomeDBiP25740.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVAFCLYKY FPFGGLQRDF MRIASTVAAR GHHVRVYTQS WEGDCPKAFE
60 70 80 90 100
LIQVPVKSHT NHGRNAEYYA WVQNHLKEHP ADRVVGFNKM PGLDVYFAAD
110 120 130 140 150
VCYAEKVAQE KGFLYRLTSR YRHYAAFERA TFEQGKSTKL MMLTDKQIAD
160 170 180 190 200
FQKHYQTEPE RFQILPPGIY PDRKYSEQIP NSREIYRQKN GIKEQQNLLL
210 220 230 240 250
QVGSDFGRKG VDRSIEALAS LPESLRHNTL LFVVGQDKPR KFEALAEKLG
260 270 280 290 300
VRSNVHFFSG RNDVSELMAA ADLLLHPAYQ EAAGIVLLEA ITAGLPVLTT
310 320 330 340 350
AVCGYAHYIA DANCGTVIAE PFSQEQLNEV LRKALTQSPL RMAWAENARH
360 370
YADTQDLYSL PEKAADIITG GLDG
Length:374
Mass (Da):42,284
Last modified:May 1, 1992 - v1
Checksum:i7F20AE577CBB80C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80599 Genomic DNA. Translation: AAA24082.1.
M86305 Genomic DNA. Translation: AAA03743.2.
U00039 Genomic DNA. Translation: AAB18608.1.
U00096 Genomic DNA. Translation: AAC76655.1.
AP009048 Genomic DNA. Translation: BAE77661.1.
S75736 Genomic DNA. Translation: AAD43826.1.
PIRiB42595.
RefSeqiNP_418088.1. NC_000913.3.
WP_000634283.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76655; AAC76655; b3631.
BAE77661; BAE77661; BAE77661.
GeneIDi948149.
KEGGiecj:JW3606.
eco:b3631.
PATRICi32122749. VBIEscCol129921_3751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80599 Genomic DNA. Translation: AAA24082.1.
M86305 Genomic DNA. Translation: AAA03743.2.
U00039 Genomic DNA. Translation: AAB18608.1.
U00096 Genomic DNA. Translation: AAC76655.1.
AP009048 Genomic DNA. Translation: BAE77661.1.
S75736 Genomic DNA. Translation: AAD43826.1.
PIRiB42595.
RefSeqiNP_418088.1. NC_000913.3.
WP_000634283.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IV7X-ray1.60A1-374[»]
2IW1X-ray1.50A1-374[»]
2N58NMR-A103-132[»]
ProteinModelPortaliP25740.
SMRiP25740. Positions 2-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260869. 274 interactions.
IntActiP25740. 10 interactions.
STRINGi511145.b3631.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

PaxDbiP25740.
PRIDEiP25740.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76655; AAC76655; b3631.
BAE77661; BAE77661; BAE77661.
GeneIDi948149.
KEGGiecj:JW3606.
eco:b3631.
PATRICi32122749. VBIEscCol129921_3751.

Organism-specific databases

EchoBASEiEB1315.
EcoGeneiEG11339. rfaG.

Phylogenomic databases

eggNOGiENOG4107RJ7. Bacteria.
ENOG410XPWS. LUCA.
HOGENOMiHOG000126226.
InParanoidiP25740.
KOiK02844.
OMAiIHICNPR.
PhylomeDBiP25740.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciEcoCyc:EG11339-MONOMER.
ECOL316407:JW3606-MONOMER.
MetaCyc:EG11339-MONOMER.
BRENDAi2.4.1.B64. 2026.

Miscellaneous databases

EvolutionaryTraceiP25740.
PROiP25740.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFAG_ECOLI
AccessioniPrimary (citable) accession number: P25740
Secondary accession number(s): Q2M7U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.