Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lysine-specific permease

Gene

lysP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Permease that is involved in the transport across the cytoplasmic membrane of lysine.

GO - Molecular functioni

  • amino acid transmembrane transporter activity Source: EcoCyc
  • antiporter activity Source: GO_Central
  • identical protein binding Source: EcoCyc
  • L-amino acid transmembrane transporter activity Source: GO_Central
  • protein heterodimerization activity Source: EcoCyc

GO - Biological processi

  • protein homooligomerization Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:LYSP-MONOMER.
ECOL316407:JW2143-MONOMER.
MetaCyc:LYSP-MONOMER.

Protein family/group databases

TCDBi2.A.3.1.2. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine-specific permease
Gene namesi
Name:lysP
Synonyms:cadR
Ordered Locus Names:b2156, JW2143
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11337. lysP.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 2120CytoplasmicCuratedAdd
BLAST
Transmembranei22 – 4221HelicalCuratedAdd
BLAST
Topological domaini43 – 5513PeriplasmicCuratedAdd
BLAST
Transmembranei56 – 7621HelicalCuratedAdd
BLAST
Topological domaini77 – 10428CytoplasmicCuratedAdd
BLAST
Transmembranei105 – 12521HelicalCuratedAdd
BLAST
Topological domaini126 – 1305PeriplasmicCurated
Transmembranei131 – 14919HelicalCuratedAdd
BLAST
Topological domaini150 – 16011CytoplasmicCuratedAdd
BLAST
Transmembranei161 – 18121HelicalCuratedAdd
BLAST
Topological domaini182 – 19918PeriplasmicCuratedAdd
BLAST
Transmembranei200 – 21819HelicalCuratedAdd
BLAST
Topological domaini219 – 24426CytoplasmicCuratedAdd
BLAST
Transmembranei245 – 26420HelicalCuratedAdd
BLAST
Topological domaini265 – 28319PeriplasmicCuratedAdd
BLAST
Transmembranei284 – 30421HelicalCuratedAdd
BLAST
Topological domaini305 – 34743CytoplasmicCuratedAdd
BLAST
Transmembranei348 – 36518HelicalCuratedAdd
BLAST
Topological domaini366 – 3716PeriplasmicCurated
Transmembranei372 – 39221HelicalCuratedAdd
BLAST
Topological domaini393 – 41523CytoplasmicCuratedAdd
BLAST
Transmembranei416 – 43318HelicalCuratedAdd
BLAST
Topological domaini434 – 44916PeriplasmicCuratedAdd
BLAST
Transmembranei450 – 47021HelicalCuratedAdd
BLAST
Topological domaini471 – 48919CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 489488Lysine-specific permeasePRO_0000054204Add
BLAST

Proteomic databases

PaxDbiP25737.

Expressioni

Inductioni

High, in anaerobic media of low pH, containing lysine.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: EcoCyc
  • protein heterodimerization activity Source: EcoCyc

Protein-protein interaction databases

BioGridi4260458. 12 interactions.
IntActiP25737. 1 interaction.
STRINGi511145.b2156.

Structurei

3D structure databases

ProteinModelPortaliP25737.
SMRiP25737. Positions 144-464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108IQI. Bacteria.
COG0833. LUCA.
HOGENOMiHOG000261850.
InParanoidiP25737.
KOiK11733.
OMAiDIPGFYW.
OrthoDBiEOG6HQSQM.
PhylomeDBiP25737.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25737-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSETKTTEA PGLRRELKAR HLTMIAIGGS IGTGLFVASG ATISQAGPGG
60 70 80 90 100
ALLSYMLIGL MVYFLMTSLG ELAAYMPVSG SFATYGQNYV EEGFGFALGW
110 120 130 140 150
NYWYNWAVTI AVDLVAAQLV MSWWFPDTPG WIWSALFLGV IFLLNYISVR
160 170 180 190 200
GFGEAEYWFS LIKVTTVIVF IIVGVLMIIG IFKGAQPAGW SNWTIGEAPF
210 220 230 240 250
AGGFAAMIGV AMIVGFSFQG TELIGIAAGE SEDPAKNIPR AVRQVFWRIL
260 270 280 290 300
LFYVFAILII SLIIPYTDPS LLRNDVKDIS VSPFTLVFQH AGLLSAAAVM
310 320 330 340 350
NAVILTAVLS AGNSGMYAST RMLYTLACDG KAPRIFAKLS RGGVPRNALY
360 370 380 390 400
ATTVIAGLCF LTSMFGNQTV YLWLLNTSGM TGFIAWLGIA ISHYRFRRGY
410 420 430 440 450
VLQGHDINDL PYRSGFFPLG PIFAFILCLI ITLGQNYEAF LKDTIDWGGV
460 470 480
AATYIGIPLF LIIWFGYKLI KGTHFVRYSE MKFPQNDKK
Length:489
Mass (Da):53,576
Last modified:January 23, 2007 - v5
Checksum:i144FAFC01F2F8E7A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1221S → N in AAA60532 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89774 Genomic DNA. Translation: AAA17053.1.
U00007 Genomic DNA. Translation: AAA60532.1.
U00096 Genomic DNA. Translation: AAC75217.1.
AP009048 Genomic DNA. Translation: BAE76633.1.
PIRiC64984.
RefSeqiNP_416661.1. NC_000913.3.
WP_000253273.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC75217; AAC75217; b2156.
BAE76633; BAE76633; BAE76633.
GeneIDi946667.
KEGGiecj:JW2143.
eco:b2156.
PATRICi32119661. VBIEscCol129921_2241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89774 Genomic DNA. Translation: AAA17053.1.
U00007 Genomic DNA. Translation: AAA60532.1.
U00096 Genomic DNA. Translation: AAC75217.1.
AP009048 Genomic DNA. Translation: BAE76633.1.
PIRiC64984.
RefSeqiNP_416661.1. NC_000913.3.
WP_000253273.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP25737.
SMRiP25737. Positions 144-464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260458. 12 interactions.
IntActiP25737. 1 interaction.
STRINGi511145.b2156.

Protein family/group databases

TCDBi2.A.3.1.2. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbiP25737.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75217; AAC75217; b2156.
BAE76633; BAE76633; BAE76633.
GeneIDi946667.
KEGGiecj:JW2143.
eco:b2156.
PATRICi32119661. VBIEscCol129921_2241.

Organism-specific databases

EchoBASEiEB1313.
EcoGeneiEG11337. lysP.

Phylogenomic databases

eggNOGiENOG4108IQI. Bacteria.
COG0833. LUCA.
HOGENOMiHOG000261850.
InParanoidiP25737.
KOiK11733.
OMAiDIPGFYW.
OrthoDBiEOG6HQSQM.
PhylomeDBiP25737.

Enzyme and pathway databases

BioCyciEcoCyc:LYSP-MONOMER.
ECOL316407:JW2143-MONOMER.
MetaCyc:LYSP-MONOMER.

Miscellaneous databases

PROiP25737.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The lysP gene encodes the lysine-specific permease."
    Steffes C., Ellis J., Wu J., Rosen B.P.
    J. Bacteriol. 174:3242-3249(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BPR2.
  2. Rosen B.P.
    Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Automated multiplex sequencing of the E.coli genome."
    Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / BHB2600.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Topological analysis of the lysine-specific permease of Escherichia coli."
    Ellis J., Carlin A., Steffes C., Wu J., Liu J., Rosen B.P.
    Microbiology 141:1927-1935(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY, PROTEIN SEQUENCE OF 2-7.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiLYSP_ECOLI
AccessioniPrimary (citable) accession number: P25737
Secondary accession number(s): Q2MAS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 136 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.