Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endonuclease-1

Gene

endA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has double-strand break activity.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.

Enzyme regulationi

Inhibited by RNA.

GO - Molecular functioni

  • deoxyribonuclease I activity Source: UniProtKB-EC
  • endodeoxyribonuclease activity, producing 5'-phosphomonoesters Source: EcoCyc
  • endonuclease activity Source: EcoCyc

GO - Biological processi

  • DNA catabolic process Source: EcoCyc
  • nucleic acid phosphodiester bond hydrolysis Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BioCyciEcoCyc:EG11336-MONOMER.
ECOL316407:JW2912-MONOMER.
MetaCyc:EG11336-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease-1 (EC:3.1.21.1)
Alternative name(s):
Endonuclease I
Short name:
Endo I
Gene namesi
Name:endA
Ordered Locus Names:b2945, JW2912
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11336. endA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 235213Endonuclease-1PRO_0000007827Add
BLAST

Proteomic databases

PaxDbiP25736.

Interactioni

Protein-protein interaction databases

BioGridi4262350. 55 interactions.
STRINGi511145.b2945.

Structurei

3D structure databases

ProteinModelPortaliP25736.
SMRiP25736. Positions 26-230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the EndA/NucM nuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105RWS. Bacteria.
COG2356. LUCA.
HOGENOMiHOG000281182.
InParanoidiP25736.
KOiK01150.
OMAiADQYGQC.
OrthoDBiEOG6DRPG6.
PhylomeDBiP25736.

Family and domain databases

InterProiIPR007346. Endonuclease-I.
[Graphical view]
PfamiPF04231. Endonuclease_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRYLSIAAV VLSAAFSGPA LAEGINSFSQ AKAAAVKVHA DAPGTFYCGC
60 70 80 90 100
KINWQGKKGV VDLQSCGYQV RKNENRASRV EWEHVVPAWQ FGHQRQCWQD
110 120 130 140 150
GGRKNCAKDP VYRKMESDMH NLQPSVGEVN GDRGNFMYSQ WNGGEGQYGQ
160 170 180 190 200
CAMKVDFKEK AAEPPARARG AIARTYFYMR DQYNLTLSRQ QTQLFNAWNK
210 220 230
MYPVTDWECE RDERIAKVQG NHNPYVQRAC QARKS
Length:235
Mass (Da):26,709
Last modified:May 1, 1992 - v1
Checksum:iEB0C355ADEF91EB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65169 Genomic DNA. Translation: CAA46287.1.
U28377 Genomic DNA. Translation: AAA69112.1.
U00096 Genomic DNA. Translation: AAC75982.1.
AP009048 Genomic DNA. Translation: BAE77008.1.
PIRiA38966.
RefSeqiNP_417420.1. NC_000913.3.
WP_000286500.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75982; AAC75982; b2945.
BAE77008; BAE77008; BAE77008.
GeneIDi949092.
KEGGiecj:JW2912.
eco:b2945.
PATRICi32121300. VBIEscCol129921_3038.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65169 Genomic DNA. Translation: CAA46287.1.
U28377 Genomic DNA. Translation: AAA69112.1.
U00096 Genomic DNA. Translation: AAC75982.1.
AP009048 Genomic DNA. Translation: BAE77008.1.
PIRiA38966.
RefSeqiNP_417420.1. NC_000913.3.
WP_000286500.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP25736.
SMRiP25736. Positions 26-230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262350. 55 interactions.
STRINGi511145.b2945.

Proteomic databases

PaxDbiP25736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75982; AAC75982; b2945.
BAE77008; BAE77008; BAE77008.
GeneIDi949092.
KEGGiecj:JW2912.
eco:b2945.
PATRICi32121300. VBIEscCol129921_3038.

Organism-specific databases

EchoBASEiEB1312.
EcoGeneiEG11336. endA.

Phylogenomic databases

eggNOGiENOG4105RWS. Bacteria.
COG2356. LUCA.
HOGENOMiHOG000281182.
InParanoidiP25736.
KOiK01150.
OMAiADQYGQC.
OrthoDBiEOG6DRPG6.
PhylomeDBiP25736.

Enzyme and pathway databases

BioCyciEcoCyc:EG11336-MONOMER.
ECOL316407:JW2912-MONOMER.
MetaCyc:EG11336-MONOMER.

Miscellaneous databases

PROiP25736.

Family and domain databases

InterProiIPR007346. Endonuclease-I.
[Graphical view]
PfamiPF04231. Endonuclease_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The periplasmic endonuclease I of Escherichia coli has amino-acid sequence homology to the extracellular DNases of Vibrio cholerae and Aeromonas hydrophila."
    Jekel M., Wackernagel W.
    Gene 154:55-59(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: SUBCELLULAR LOCATION.
  5. "The deoxyribonuclease of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease."
    Lehman I.R., Roussos G.G., Pratt E.A.
    J. Biol. Chem. 237:819-828(1962) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "The deoxyribonuclease of Escherichia coli. III. Studies on the nature of the inhibition of endonuclease by ribonucleic acid."
    Lehman I.R., Roussos G.G., Pratt E.A.
    J. Biol. Chem. 237:829-833(1962) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome."
    Jekel M., Wackernagel W.
    J. Bacteriol. 176:1550-1551(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE MAPPING.

Entry informationi

Entry nameiEND1_ECOLI
AccessioniPrimary (citable) accession number: P25736
Secondary accession number(s): Q2M9P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 13, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.