P25736 (END1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endonuclease-1
EC=3.1.21.1

Alternative name(s):
Endonuclease I
Short name=Endo I

Gene names

Name:	endA
Ordered Locus Names:	b2945, JW2912

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has double-strand break activity.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.
Enzyme regulation	Inhibited by RNA.
Subcellular location	Periplasm Ref.4.
Sequence similarities	Belongs to the endA/nucM nuclease family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	deoxyribonuclease I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 235	213	Endonuclease-1		PRO_0000007827

Sequences

	Sequence		Length	Mass (Da)
	P25736 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: EB0C355ADEF91EB3	FASTA	235	26,709
	10 20 30 40 50 60 MYRYLSIAAV VLSAAFSGPA LAEGINSFSQ AKAAAVKVHA DAPGTFYCGC KINWQGKKGV 70 80 90 100 110 120 VDLQSCGYQV RKNENRASRV EWEHVVPAWQ FGHQRQCWQD GGRKNCAKDP VYRKMESDMH 130 140 150 160 170 180 NLQPSVGEVN GDRGNFMYSQ WNGGEGQYGQ CAMKVDFKEK AAEPPARARG AIARTYFYMR 190 200 210 220 230 DQYNLTLSRQ QTQLFNAWNK MYPVTDWECE RDERIAKVQG NHNPYVQRAC QARKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The periplasmic endonuclease I of Escherichia coli has amino-acid sequence homology to the extracellular DNases of Vibrio cholerae and Aeromonas hydrophila." Jekel M., Wackernagel W. Gene 154:55-59(1995) [PubMed: 7867949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Localization of E. coli endonuclease I." Cordonnier C., Bernardi G. Biochem. Biophys. Res. Commun. 20:555-559(1965) [PubMed: 4955462] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"The deoxyribonuclease of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease." Lehman I.R., Roussos G.G., Pratt E.A. J. Biol. Chem. 237:819-828(1962) [PubMed: 14463751] [Abstract] Cited for: CHARACTERIZATION.
[6]	"The deoxyribonuclease of Escherichia coli. III. Studies on the nature of the inhibition of endonuclease by ribonucleic acid." Lehman I.R., Roussos G.G., Pratt E.A. J. Biol. Chem. 237:829-833(1962) [PubMed: 14463750] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome." Jekel M., Wackernagel W. J. Bacteriol. 176:1550-1551(1994) [PubMed: 8113204] [Abstract] Cited for: GENE MAPPING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X65169 Genomic DNA. Translation: CAA46287.1. U28377 Genomic DNA. Translation: AAA69112.1. U00096 Genomic DNA. Translation: AAC75982.1. AP009048 Genomic DNA. Translation: BAE77008.1.
PIR	A38966.
RefSeq	NP_417420.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P25736.
SMR	P25736. Positions 26-230.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002024; EBESCP00000002024; EBESCG00000001649. EBESCT00000002025; EBESCP00000002025; EBESCG00000001649. EBESCT00000015150; EBESCP00000014441; EBESCG00000014210.
GeneID	949092.
GenomeReviews	Gene locus JW2912 in contig AP009048_GR. Gene locus b2945 in contig U00096_GR.
KEGG	ecj:JW2912. eco:b2945.
PATRIC	32121300. VBIEscCol129921_3038.
Organism-specific databases
EchoBASE	EB1312.
EcoGene	EG11336. endA.
Phylogenomic databases
eggNOG	COG2356.
GeneTree	EBGT00050000009475.
HOGENOM	HBG297976.
OMA	IECKRDN.
ProtClustDB	PRK15137.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11336-MONOMER. MetaCyc:EG11336-MONOMER.
Gene expression databases
Genevestigator	P25736.
Family and domain databases
InterPro	IPR007346. Endonuclease-I. [Graphical view]
KO	K01150.
Pfam	PF04231. Endonuclease_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

END1_ECOLI

Accession

Primary (citable) accession number: P25736
Secondary accession number(s): Q2M9P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program