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Protein

Dorsal-ventral patterning protein tolloid

Gene

tld

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for normal dorsal development. TLD may interact physically with DPP-C protein.

Miscellaneous

Mutations in TLD lead to a partial transformation of dorsal ectoderm into ventral ectoderm.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi231Zinc; catalyticPROSITE-ProRule annotation1
Active sitei232PROSITE-ProRule annotation1
Metal bindingi235Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi241Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • metalloendopeptidase activity Source: FlyBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • amnioserosa formation Source: FlyBase
  • embryonic pattern specification Source: FlyBase
  • imaginal disc-derived wing vein morphogenesis Source: FlyBase
  • maternal specification of dorsal/ventral axis, oocyte, soma encoded Source: FlyBase
  • negative regulation of gene expression Source: FlyBase
  • positive regulation of BMP signaling pathway Source: FlyBase
  • protein processing Source: FlyBase
  • proteolysis Source: FlyBase
  • regulation of transforming growth factor beta receptor signaling pathway Source: FlyBase
  • terminal region determination Source: FlyBase
  • torso signaling pathway Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, Hydrolase, Metalloprotease, Protease
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-1474228 Degradation of the extracellular matrix
R-DME-2243919 Crosslinking of collagen fibrils

Protein family/group databases

MEROPSiM12.010

Names & Taxonomyi

Protein namesi
Recommended name:
Dorsal-ventral patterning protein tolloid (EC:3.4.24.-)
Gene namesi
Name:tld
ORF Names:CG6868
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003719 tld

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Sequence analysisAdd BLAST36
PropeptideiPRO_000002890337 – 136Sequence analysisAdd BLAST100
ChainiPRO_0000028904137 – 1067Dorsal-ventral patterning protein tolloidAdd BLAST931

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi176N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi340 ↔ 390By similarity
Disulfide bondi417 ↔ 439By similarity
Glycosylationi441N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi478 ↔ 505By similarity
Disulfide bondi532 ↔ 554By similarity
Glycosylationi543N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi595 ↔ 606By similarity
Disulfide bondi602 ↔ 615By similarity
Disulfide bondi617 ↔ 630By similarity
Disulfide bondi634 ↔ 662By similarity
Glycosylationi644N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi677N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi693 ↔ 716By similarity
Disulfide bondi757 ↔ 768By similarity
Disulfide bondi764 ↔ 777By similarity
Disulfide bondi779 ↔ 792By similarity
Glycosylationi791N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi797 ↔ 823By similarity
Disulfide bondi850 ↔ 872By similarity
Glycosylationi864N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi910 ↔ 940By similarity
Glycosylationi918N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi967 ↔ 989By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP25723
PRIDEiP25723

Expressioni

Gene expression databases

BgeeiFBgn0003719
ExpressionAtlasiP25723 baseline and differential
GenevisibleiP25723 DM

Interactioni

Protein-protein interaction databases

BioGridi67870, 7 interactors
DIPiDIP-22889N
IntActiP25723, 1 interactor
STRINGi7227.FBpp0084070

Structurei

3D structure databases

ProteinModelPortaliP25723
SMRiP25723
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini340 – 477CUB 1PROSITE-ProRule annotationAdd BLAST138
Domaini478 – 591CUB 2PROSITE-ProRule annotationAdd BLAST114
Domaini591 – 631EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini634 – 753CUB 3PROSITE-ProRule annotationAdd BLAST120
Domaini753 – 793EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST41
Domaini797 – 909CUB 4PROSITE-ProRule annotationAdd BLAST113
Domaini910 – 1026CUB 5PROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 339MetalloproteaseAdd BLAST203

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi245 – 247Cell attachment siteSequence analysis3
Motifi325 – 327Cell attachment siteSequence analysis3

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3714 Eukaryota
ENOG410ZPX7 LUCA
InParanoidiP25723
KOiK13045
OMAiKGFEASH
OrthoDBiEOG091G009U
PhylomeDBiP25723

Family and domain databases

CDDicd00041 CUB, 5 hits
cd04281 ZnMc_BMP1_TLD, 1 hit
Gene3Di2.60.120.290, 5 hits
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR015446 BMP_1/tolloid-like
IPR000859 CUB_dom
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR024079 MetalloPept_cat_dom_sf
IPR001506 Peptidase_M12A
IPR006026 Peptidase_Metallo
IPR035914 Sperma_CUB_dom_sf
IPR034036 ZnMP_TLD/BMP1
PfamiView protein in Pfam
PF01400 Astacin, 1 hit
PF00431 CUB, 5 hits
PF07645 EGF_CA, 1 hit
PIRSFiPIRSF001199 BMP_1/tolloid-like, 1 hit
PRINTSiPR00480 ASTACIN
SMARTiView protein in SMART
SM00042 CUB, 5 hits
SM00181 EGF, 2 hits
SM00179 EGF_CA, 2 hits
SM00235 ZnMc, 1 hit
SUPFAMiSSF49854 SSF49854, 6 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 2 hits
PS01180 CUB, 5 hits
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 2 hits
PS01187 EGF_CA, 2 hits
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25723-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGMRLMPMK MKAKLVVLSV GALWMMMFFL VDYAEGRRLS QLPESECDFD
60 70 80 90 100
FKEQPEDFFG ILDSSLVPPK EPKDDIYQLK TTRQHSGRRR KQSHKSQNKA
110 120 130 140 150
ALRLPPPFLW TDDAVDVLQH SHSPTLNGQP IQRRRRAVTV RKERTWDYGV
160 170 180 190 200
IPYEIDTIFS GAHKALFKQA MRHWENFTCI KFVERDPNLH ANYIYFTVKN
210 220 230 240 250
CGCCSFLGKN GNGRQPISIG RNCEKFGIII HELGHTIGFH HEHARGDRDK
260 270 280 290 300
HIVINKGNIM RGQEYNFDVL SPEEVDLPLL PYDLNSIMHY AKNSFSKSPY
310 320 330 340 350
LDTITPIGIP PGTHLELGQR KRLSRGDIVQ ANLLYKCASC GRTYQQNSGH
360 370 380 390 400
IVSPHFIYSG NGVLSEFEGS GDAGEDPSAE SEFDASLTNC EWRITATNGE
410 420 430 440 450
KVILHLQQLH LMSSDDCTQD YLEIRDGYWH KSPLVRRICG NVSGEVITTQ
460 470 480 490 500
TSRMLLNYVN RNAAKGYRGF KARFEVVCGG DLKLTKDQSI DSPNYPMDYM
510 520 530 540 550
PDKECVWRIT APDNHQVALK FQSFELEKHD GCAYDFVEIR DGNHSDSRLI
560 570 580 590 600
GRFCGDKLPP NIKTRSNQMY IRFVSDSSVQ KLGFSAALML DVDECKFTDH
610 620 630 640 650
GCQHLCINTL GSYQCGCRAG YELQANGKTC EDACGGVVDA TKSNGSLYSP
660 670 680 690 700
SYPDVYPNSK QCVWEVVAPP NHAVFLNFSH FDLEGTRFHY TKCNYDYLII
710 720 730 740 750
YSKMRDNRLK KIGIYCGHEL PPVVNSEQSI LRLEFYSDRT VQRSGFVAKF
760 770 780 790 800
VIDVDECSMN NGGCQHRCRN TFGSYQCSCR NGYTLAENGH NCTETRCKFE
810 820 830 840 850
ITTSYGVLQS PNYPEDYPRN IYCYWHFQTV LGHRIQLTFH DFEVESHQEC
860 870 880 890 900
IYDYVAIYDG RSENSSTLGI YCGGREPYAV IASTNEMFMV LATDAGLQRK
910 920 930 940 950
GFKATFVSEC GGYLRATNHS QTFYSHPRYG SRPYKRNMYC DWRIQADPES
960 970 980 990 1000
SVKIRFLHFE IEYSERCDYD YLEITEEGYS MNTIHGRFCG KHKPPIIISN
1010 1020 1030 1040 1050
SDTLLLRFQT DESNSLRGFA ISFMAVDPPE DSVGEDFDAV TPFPGYLKSM
1060
YSSETGSDHL LPPSRLI
Length:1,067
Mass (Da):121,780
Last modified:August 16, 2004 - v2
Checksum:i6375871E9F2B54C2
GO

Sequence cautioni

The sequence AAA28491 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAC46482 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76976 mRNA Translation: AAA28491.1 Different initiation.
U04239 Unassigned DNA Translation: AAC46482.1 Different initiation.
AE014297 Genomic DNA Translation: AAF56329.2
PIRiA39288
RefSeqiNP_524487.2, NM_079763.4

Genome annotation databases

EnsemblMetazoaiFBtr0084691; FBpp0084070; FBgn0003719
GeneIDi42945
KEGGidme:Dmel_CG6868
UCSCiCG6868-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiTLD_DROME
AccessioniPrimary (citable) accession number: P25723
Secondary accession number(s): Q9VC46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: August 16, 2004
Last modified: May 23, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health