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P25718 (AMY1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-amylase

EC=3.2.1.1
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene names
Name:malS
Ordered Locus Names:b3571, JW3543
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length676 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded by the periplasmic alpha-amylase, the MalS protein.

Catalytic activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subunit structure

Monomer.

Subcellular location

Periplasm.

Induction

Under the regulatory control of the MalT protein.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Ref.1
Chain18 – 676659Alpha-amylase
PRO_0000001339

Sites

Active site4601Nucleophile By similarity
Active site5031Proton donor By similarity
Metal binding3141Calcium By similarity
Metal binding4641Calcium; via carbonyl oxygen By similarity
Site5651Transition state stabilizer By similarity

Amino acid modifications

Disulfide bond57 ↔ 75 Ref.5
Disulfide bond121 ↔ 537 Ref.5

Sequences

Sequence LengthMass (Da)Tools
P25718 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 980110EFA45E011D

FASTA67675,713
        10         20         30         40         50         60 
MKLAACFLTL LPGFAVAASW TSPGFPAFSE QGTGTFVSHA QLPKGTRPLT LNFDQQCWQP 

        70         80         90        100        110        120 
ADAIKLNQML SLQPCSNTPP QWRLFRDGEY TLQIDTRSGT PTLMISIQNA AEPVASLVRE 

       130        140        150        160        170        180 
CPKWDGLPLT VDVSATFPEG AAVRDYYSQQ IAIVKNGQIM LQPAATSNGL LLLERAETDT 

       190        200        210        220        230        240 
SAPFDWHNAT VYFVLTDRFE NGDPSNDQSY GRHKDGMAEI GTFHGGDLRG LTNKLDYLQQ 

       250        260        270        280        290        300 
LGVNALWISA PFEQIHGWVG GGTKGDFPHY AYHGYYTQDW TNLDANMGNE ADLRTLVDSA 

       310        320        330        340        350        360 
HQRGIRILFD VVMNHTGYAT LADMQEYQFG ALYLSGDEVK KSLGERWSDW KPAAGQTWHS 

       370        380        390        400        410        420 
FNDYINFSDK TGWDKWWGKN WIRTDIGDYD NPGFDDLTMS LAFLPDIKTE STTASGLPVF 

       430        440        450        460        470        480 
YKNKMDTHAK AIDGYTPRDY LTHWLSQWVR DYGIDGFRVD TAKHVELPAW QQLKTEASAA 

       490        500        510        520        530        540 
LREWKKANPD KALDDKPFWM TGEAWGHGVM QSDYYRHGFD AMINFDYQEQ AAKAVDCLAQ 

       550        560        570        580        590        600 
MDTTWQQMAE KLQGFNVLSY LSSHDTRLFR EGGDKAAELL LLAPGAVQIF YGDESSRPFG 

       610        620        630        640        650        660 
PTGSDPLQGT RSDMNWQDVS GKSAASVAHW QKISQFRARH PAIGAGKQTT LLLKQGYGFV 

       670 
REHGDDKVLV VWAGQQ 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by malS."
Schneider E., Freundlieb S., Tapio S., Boos W.
J. Biol. Chem. 267:5148-5154(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-43.
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Biochemical characterization and mass spectrometric disulfide bond mapping of periplasmic alpha-amylase MalS of Escherichia coli."
Spiess C., Happersberger H.P., Glocker M.O., Spiess E., Rippe K., Ehrmann M.
J. Biol. Chem. 272:22125-22133(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X58994 Genomic DNA. Translation: CAA41740.1.
U00039 Genomic DNA. Translation: AAB18548.1.
U00096 Genomic DNA. Translation: AAC76595.1.
AP009048 Genomic DNA. Translation: BAE77722.1.
PIRS23807.
RefSeqNP_418028.1. NC_000913.3.
YP_491863.1. NC_007779.1.

3D structure databases

ProteinModelPortalP25718.
SMRP25718. Positions 181-670.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10148N.
IntActP25718. 1 interaction.
MINTMINT-1293484.
STRING511145.b3571.

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEP25718.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76595; AAC76595; b3571.
BAE77722; BAE77722; BAE77722.
GeneID12932718.
948088.
KEGGecj:Y75_p3604.
eco:b3571.
PATRIC32122618. VBIEscCol129921_3686.

Organism-specific databases

EchoBASEEB1292.
EcoGeneEG11316. malS.

Phylogenomic databases

eggNOGCOG0366.
HOGENOMHOG000273912.
KOK01176.
OMAGQNWHSF.
OrthoDBEOG65QWH3.
PhylomeDBP25718.
ProtClustDBPRK09505.

Enzyme and pathway databases

BioCycEcoCyc:ALPHA-AMYL-PERI-MONOMER.
ECOL316407:JW3543-MONOMER.
MetaCyc:ALPHA-AMYL-PERI-MONOMER.

Gene expression databases

GenevestigatorP25718.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR014635. A_amylase_MalS.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFPIRSF036917. Alph_amls_MalS. 1 hit.
SMARTSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 2 hits.
ProtoNetSearch...

Other

PROP25718.

Entry information

Entry nameAMY1_ECOLI
AccessionPrimary (citable) accession number: P25718
Secondary accession number(s): Q2M7N4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene