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P25718

- AMY1_ECOLI

UniProt

P25718 - AMY1_ECOLI

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Protein

Alpha-amylase

Gene

malS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded by the periplasmic alpha-amylase, the MalS protein.

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

Cofactori

Binds 1 calcium ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi314 – 3141CalciumBy similarity
Active sitei460 – 4601NucleophileBy similarity
Metal bindingi464 – 4641Calcium; via carbonyl oxygenBy similarity
Active sitei503 – 5031Proton donorBy similarity
Sitei565 – 5651Transition state stabilizerBy similarity

GO - Molecular functioni

  1. alpha-amylase activity Source: EcoCyc
  2. calcium ion binding Source: EcoCyc

GO - Biological processi

  1. alpha-glucan catabolic process Source: EcoCyc
  2. cellular oligosaccharide catabolic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:ALPHA-AMYL-PERI-MONOMER.
ECOL316407:JW3543-MONOMER.
MetaCyc:ALPHA-AMYL-PERI-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase (EC:3.2.1.1)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:malS
Ordered Locus Names:b3571, JW3543
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11316. malS.

Subcellular locationi

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: EcoCyc
  2. periplasmic space Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 PublicationAdd
BLAST
Chaini18 – 676659Alpha-amylasePRO_0000001339Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 751 Publication
Disulfide bondi121 ↔ 5371 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP25718.

Expressioni

Inductioni

Under the regulatory control of the MalT protein.

Gene expression databases

GenevestigatoriP25718.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-10148N.
IntActiP25718. 1 interaction.
MINTiMINT-1293484.
STRINGi511145.b3571.

Structurei

3D structure databases

ProteinModelPortaliP25718.
SMRiP25718. Positions 181-670.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0366.
HOGENOMiHOG000273912.
InParanoidiP25718.
KOiK01176.
OMAiGQNWHSF.
OrthoDBiEOG65QWH3.
PhylomeDBiP25718.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR014635. A_amylase_MalS.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036917. Alph_amls_MalS. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25718-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLAACFLTL LPGFAVAASW TSPGFPAFSE QGTGTFVSHA QLPKGTRPLT
60 70 80 90 100
LNFDQQCWQP ADAIKLNQML SLQPCSNTPP QWRLFRDGEY TLQIDTRSGT
110 120 130 140 150
PTLMISIQNA AEPVASLVRE CPKWDGLPLT VDVSATFPEG AAVRDYYSQQ
160 170 180 190 200
IAIVKNGQIM LQPAATSNGL LLLERAETDT SAPFDWHNAT VYFVLTDRFE
210 220 230 240 250
NGDPSNDQSY GRHKDGMAEI GTFHGGDLRG LTNKLDYLQQ LGVNALWISA
260 270 280 290 300
PFEQIHGWVG GGTKGDFPHY AYHGYYTQDW TNLDANMGNE ADLRTLVDSA
310 320 330 340 350
HQRGIRILFD VVMNHTGYAT LADMQEYQFG ALYLSGDEVK KSLGERWSDW
360 370 380 390 400
KPAAGQTWHS FNDYINFSDK TGWDKWWGKN WIRTDIGDYD NPGFDDLTMS
410 420 430 440 450
LAFLPDIKTE STTASGLPVF YKNKMDTHAK AIDGYTPRDY LTHWLSQWVR
460 470 480 490 500
DYGIDGFRVD TAKHVELPAW QQLKTEASAA LREWKKANPD KALDDKPFWM
510 520 530 540 550
TGEAWGHGVM QSDYYRHGFD AMINFDYQEQ AAKAVDCLAQ MDTTWQQMAE
560 570 580 590 600
KLQGFNVLSY LSSHDTRLFR EGGDKAAELL LLAPGAVQIF YGDESSRPFG
610 620 630 640 650
PTGSDPLQGT RSDMNWQDVS GKSAASVAHW QKISQFRARH PAIGAGKQTT
660 670
LLLKQGYGFV REHGDDKVLV VWAGQQ
Length:676
Mass (Da):75,713
Last modified:May 1, 1992 - v1
Checksum:i980110EFA45E011D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58994 Genomic DNA. Translation: CAA41740.1.
U00039 Genomic DNA. Translation: AAB18548.1.
U00096 Genomic DNA. Translation: AAC76595.1.
AP009048 Genomic DNA. Translation: BAE77722.1.
PIRiS23807.
RefSeqiNP_418028.1. NC_000913.3.
YP_491863.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76595; AAC76595; b3571.
BAE77722; BAE77722; BAE77722.
GeneIDi12932718.
948088.
KEGGiecj:Y75_p3604.
eco:b3571.
PATRICi32122618. VBIEscCol129921_3686.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X58994 Genomic DNA. Translation: CAA41740.1 .
U00039 Genomic DNA. Translation: AAB18548.1 .
U00096 Genomic DNA. Translation: AAC76595.1 .
AP009048 Genomic DNA. Translation: BAE77722.1 .
PIRi S23807.
RefSeqi NP_418028.1. NC_000913.3.
YP_491863.1. NC_007779.1.

3D structure databases

ProteinModelPortali P25718.
SMRi P25718. Positions 181-670.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-10148N.
IntActi P25718. 1 interaction.
MINTi MINT-1293484.
STRINGi 511145.b3571.

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEi P25718.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76595 ; AAC76595 ; b3571 .
BAE77722 ; BAE77722 ; BAE77722 .
GeneIDi 12932718.
948088.
KEGGi ecj:Y75_p3604.
eco:b3571.
PATRICi 32122618. VBIEscCol129921_3686.

Organism-specific databases

EchoBASEi EB1292.
EcoGenei EG11316. malS.

Phylogenomic databases

eggNOGi COG0366.
HOGENOMi HOG000273912.
InParanoidi P25718.
KOi K01176.
OMAi GQNWHSF.
OrthoDBi EOG65QWH3.
PhylomeDBi P25718.

Enzyme and pathway databases

BioCyci EcoCyc:ALPHA-AMYL-PERI-MONOMER.
ECOL316407:JW3543-MONOMER.
MetaCyc:ALPHA-AMYL-PERI-MONOMER.

Miscellaneous databases

PROi P25718.

Gene expression databases

Genevestigatori P25718.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR014635. A_amylase_MalS.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036917. Alph_amls_MalS. 1 hit.
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by malS."
    Schneider E., Freundlieb S., Tapio S., Boos W.
    J. Biol. Chem. 267:5148-5154(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-43.
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Biochemical characterization and mass spectrometric disulfide bond mapping of periplasmic alpha-amylase MalS of Escherichia coli."
    Spiess C., Happersberger H.P., Glocker M.O., Spiess E., Rippe K., Ehrmann M.
    J. Biol. Chem. 272:22125-22133(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BONDS.

Entry informationi

Entry nameiAMY1_ECOLI
AccessioniPrimary (citable) accession number: P25718
Secondary accession number(s): Q2M7N4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 29, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3