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P25718

- AMY1_ECOLI

UniProt

P25718 - AMY1_ECOLI

Protein

Alpha-amylase

Gene

malS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded by the periplasmic alpha-amylase, the MalS protein.

    Catalytic activityi

    Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi314 – 3141CalciumBy similarity
    Active sitei460 – 4601NucleophileBy similarity
    Metal bindingi464 – 4641Calcium; via carbonyl oxygenBy similarity
    Active sitei503 – 5031Proton donorBy similarity
    Sitei565 – 5651Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. alpha-amylase activity Source: EcoCyc
    2. calcium ion binding Source: EcoCyc

    GO - Biological processi

    1. alpha-glucan catabolic process Source: EcoCyc
    2. cellular oligosaccharide catabolic process Source: EcoliWiki

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:ALPHA-AMYL-PERI-MONOMER.
    ECOL316407:JW3543-MONOMER.
    MetaCyc:ALPHA-AMYL-PERI-MONOMER.

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-amylase (EC:3.2.1.1)
    Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
    Gene namesi
    Name:malS
    Ordered Locus Names:b3571, JW3543
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11316. malS.

    Subcellular locationi

    GO - Cellular componenti

    1. outer membrane-bounded periplasmic space Source: EcoCyc
    2. periplasmic space Source: EcoliWiki

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 17171 PublicationAdd
    BLAST
    Chaini18 – 676659Alpha-amylasePRO_0000001339Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi57 ↔ 751 Publication
    Disulfide bondi121 ↔ 5371 Publication

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP25718.

    Expressioni

    Inductioni

    Under the regulatory control of the MalT protein.

    Gene expression databases

    GenevestigatoriP25718.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    DIPiDIP-10148N.
    IntActiP25718. 1 interaction.
    MINTiMINT-1293484.
    STRINGi511145.b3571.

    Structurei

    3D structure databases

    ProteinModelPortaliP25718.
    SMRiP25718. Positions 181-670.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0366.
    HOGENOMiHOG000273912.
    KOiK01176.
    OMAiGQNWHSF.
    OrthoDBiEOG65QWH3.
    PhylomeDBiP25718.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR014635. A_amylase_MalS.
    IPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036917. Alph_amls_MalS. 1 hit.
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25718-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLAACFLTL LPGFAVAASW TSPGFPAFSE QGTGTFVSHA QLPKGTRPLT    50
    LNFDQQCWQP ADAIKLNQML SLQPCSNTPP QWRLFRDGEY TLQIDTRSGT 100
    PTLMISIQNA AEPVASLVRE CPKWDGLPLT VDVSATFPEG AAVRDYYSQQ 150
    IAIVKNGQIM LQPAATSNGL LLLERAETDT SAPFDWHNAT VYFVLTDRFE 200
    NGDPSNDQSY GRHKDGMAEI GTFHGGDLRG LTNKLDYLQQ LGVNALWISA 250
    PFEQIHGWVG GGTKGDFPHY AYHGYYTQDW TNLDANMGNE ADLRTLVDSA 300
    HQRGIRILFD VVMNHTGYAT LADMQEYQFG ALYLSGDEVK KSLGERWSDW 350
    KPAAGQTWHS FNDYINFSDK TGWDKWWGKN WIRTDIGDYD NPGFDDLTMS 400
    LAFLPDIKTE STTASGLPVF YKNKMDTHAK AIDGYTPRDY LTHWLSQWVR 450
    DYGIDGFRVD TAKHVELPAW QQLKTEASAA LREWKKANPD KALDDKPFWM 500
    TGEAWGHGVM QSDYYRHGFD AMINFDYQEQ AAKAVDCLAQ MDTTWQQMAE 550
    KLQGFNVLSY LSSHDTRLFR EGGDKAAELL LLAPGAVQIF YGDESSRPFG 600
    PTGSDPLQGT RSDMNWQDVS GKSAASVAHW QKISQFRARH PAIGAGKQTT 650
    LLLKQGYGFV REHGDDKVLV VWAGQQ 676
    Length:676
    Mass (Da):75,713
    Last modified:May 1, 1992 - v1
    Checksum:i980110EFA45E011D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58994 Genomic DNA. Translation: CAA41740.1.
    U00039 Genomic DNA. Translation: AAB18548.1.
    U00096 Genomic DNA. Translation: AAC76595.1.
    AP009048 Genomic DNA. Translation: BAE77722.1.
    PIRiS23807.
    RefSeqiNP_418028.1. NC_000913.3.
    YP_491863.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76595; AAC76595; b3571.
    BAE77722; BAE77722; BAE77722.
    GeneIDi12932718.
    948088.
    KEGGiecj:Y75_p3604.
    eco:b3571.
    PATRICi32122618. VBIEscCol129921_3686.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X58994 Genomic DNA. Translation: CAA41740.1 .
    U00039 Genomic DNA. Translation: AAB18548.1 .
    U00096 Genomic DNA. Translation: AAC76595.1 .
    AP009048 Genomic DNA. Translation: BAE77722.1 .
    PIRi S23807.
    RefSeqi NP_418028.1. NC_000913.3.
    YP_491863.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P25718.
    SMRi P25718. Positions 181-670.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10148N.
    IntActi P25718. 1 interaction.
    MINTi MINT-1293484.
    STRINGi 511145.b3571.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PRIDEi P25718.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76595 ; AAC76595 ; b3571 .
    BAE77722 ; BAE77722 ; BAE77722 .
    GeneIDi 12932718.
    948088.
    KEGGi ecj:Y75_p3604.
    eco:b3571.
    PATRICi 32122618. VBIEscCol129921_3686.

    Organism-specific databases

    EchoBASEi EB1292.
    EcoGenei EG11316. malS.

    Phylogenomic databases

    eggNOGi COG0366.
    HOGENOMi HOG000273912.
    KOi K01176.
    OMAi GQNWHSF.
    OrthoDBi EOG65QWH3.
    PhylomeDBi P25718.

    Enzyme and pathway databases

    BioCyci EcoCyc:ALPHA-AMYL-PERI-MONOMER.
    ECOL316407:JW3543-MONOMER.
    MetaCyc:ALPHA-AMYL-PERI-MONOMER.

    Miscellaneous databases

    PROi P25718.

    Gene expression databases

    Genevestigatori P25718.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR014635. A_amylase_MalS.
    IPR015902. Glyco_hydro_13.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036917. Alph_amls_MalS. 1 hit.
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by malS."
      Schneider E., Freundlieb S., Tapio S., Boos W.
      J. Biol. Chem. 267:5148-5154(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 18-43.
      Strain: K12.
    2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
      Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
      Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Biochemical characterization and mass spectrometric disulfide bond mapping of periplasmic alpha-amylase MalS of Escherichia coli."
      Spiess C., Happersberger H.P., Glocker M.O., Spiess E., Rippe K., Ehrmann M.
      J. Biol. Chem. 272:22125-22133(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BONDS.

    Entry informationi

    Entry nameiAMY1_ECOLI
    AccessioniPrimary (citable) accession number: P25718
    Secondary accession number(s): Q2M7N4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3