Reviewed, UniProtKB/Swiss-Prot P25714 (OXAA_ECOLI)

Names and origin

Protein names

Recommended name:
    Inner membrane protein oxaA

Gene names

Name:	oxaA
Synonyms:	yidC
Ordered Locus Names:	b3705, JW3683

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inner membrane protein required for the insertion of integral membrane proteins into the membrane. Probably plays an essential role in the integration of proteins of the respiratory chain complexes. Involved in integration of membrane proteins that insert dependently and independently of the Sec translocase complex. Essential for the integration of subunits a and c of the F1F0ATP synthase, and secE proteins. Ref.6 Ref.7 Ref.10 Ref.12
Subunit structure	Interacts with secD and secF. Component of the SecDFyajC complex, a heterotetrameric translocase complex. Specifically interacts with transmembrane segments of nascent integral membrane proteins during membrane integration. Found in 3 different complexes in inner membrane preparations (Ref.13). Ref.6 Ref.8 Ref.13
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Note: Predominantly localized at the poles of cells. Ref.13 Ref.9
Sequence similarities	Belongs to the OXA1/oxaA family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	protein insertion into membrane Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 548

548

Inner membrane protein oxaA HAMAP MF_01810

PRO_0000124712

Regions

Topological domain

1 – 5

5

Cytoplasmic Potential

Transmembrane

6 – 26

21

Potential

Topological domain

27 – 316

290

Periplasmic Potential

Transmembrane

317 – 337

21

Potential

Topological domain

338 – 349

12

Cytoplasmic Potential

Transmembrane

350 – 370

21

Potential

Topological domain

371 – 419

49

Periplasmic Potential

Transmembrane

420 – 440

21

Potential

Topological domain

441 – 457

17

Cytoplasmic Potential

Transmembrane

458 – 478

21

Potential

Topological domain

479 – 498

20

Periplasmic Potential

Transmembrane

499 – 519

21

Potential

Topological domain

520 – 548

29

Cytoplasmic Potential

Experimental info

Mutagenesis

361

1

I → S: Loss of function. Ref.11

Mutagenesis

436

1

L → S: Loss of function. Ref.11

Secondary structure

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548

Helix Strand Turn

Details...

Beta strand

60 – 64

5

Beta strand

69 – 73

5

Beta strand

78 – 90

13

Beta strand

95 – 103

9

Beta strand

106 – 116

11

Helix

123 – 125

3

Beta strand

136 – 139

4

Beta strand

145 – 154

10

Beta strand

160 – 168

9

Beta strand

173 – 181

9

Beta strand

184 – 186

3

Beta strand

188 – 201

14

Beta strand

220 – 224

5

Turn

226 – 228

3

Beta strand

231 – 233

3

Helix

235 – 239

5

Beta strand

245 – 250

6

Beta strand

252 – 257

6

Beta strand

260 – 280

21

Beta strand

283 – 289

7

Beta strand

293 – 295

3

Beta strand

300 – 311

12

Helix

314 – 320

7

Helix

324 – 327

4

Helix

330 – 336

7

Sequences

	Sequence		Length	Mass (Da)
	P25714-1 [UniParc]. Last modified July 1, 1993. Version 2. Checksum: 95EBC5DAB4F2FCFB	FASTA	548	61,526
	10 20 30 40 50 60 MDSQRNLLVI ALLFVSFMIW QAWEQDKNPQ PQAQQTTQTT TTAAGSAADQ GVPASGQGKL 70 80 90 100 110 120 ISVKTDVLDL TINTRGGDVE QALLPAYPKE LNSTQPFQLL ETSPQFIYQA QSGLTGRDGP 130 140 150 160 170 180 DNPANGPRPL YNVEKDAYVL AEGQNELQVP MTYTDAAGNT FTKTFVLKRG DYAVNVNYNV 190 200 210 220 230 240 QNAGEKPLEI SSFGQLKQSI TLPPHLDTGS SNFALHTFRG AAYSTPDEKY EKYKFDTIAD 250 260 270 280 290 300 NENLNISSKG GWVAMLQQYF ATAWIPHNDG TNNFYTANLG NGIAAIGYKS QPVLVQPGQT 310 320 330 340 350 360 GAMNSTLWVG PEIQDKMAAV APHLDLTVDY GWLWFISQPL FKLLKWIHSF VGNWGFSIII 370 380 390 400 410 420 ITFIVRGIMY PLTKAQYTSM AKMRMLQPKI QAMRERLGDD KQRISQEMMA LYKAEKVNPL 430 440 450 460 470 480 GGCFPLLIQM PIFLALYYML MGSVELRQAP FALWIHDLSA QDPYYILPIL MGVTMFFIQK 490 500 510 520 530 540 MSPTTVTDPM QQKIMTFMPV IFTVFFLWFP SGLVLYYIVS NLVTIIQQQL IYRGLEKRGL HSREKKKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Physical mapping and nucleotide sequence of the rnpA gene that encodes the protein component of ribonuclease P in Escherichia coli." Hansen F.G., Hansen E.B., Atlung T. Gene 38:85-93(1985) [PubMed: 2415431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2.
[5]	"Membrane topology of the 60-kDa Oxa1p homologue from Escherichia coli." Saeaef A., Monne M., de Gier J.W., von Heijne G. J. Biol. Chem. 273:30415-30418(1998) [PubMed: 9804807] [Abstract] Cited for: TOPOLOGY.
[6]	"YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase." Scotti P.A., Urbanus M.L., Brunner J., de Gier J.-W., von Heijne G., van der Does C., Driessen A.J.M., Oudega B., Luirink J. EMBO J. 19:542-549(2000) [PubMed: 10675323] [Abstract] Cited for: FUNCTION IN SEC-DEPENDENT PATHWAY, INTERACTION WITH TRANSMEMBRANE SEGMENTS.
[7]	"YidC mediates membrane protein insertion in bacteria." Samuelson J.C., Chen M., Jiang F., Moeller I., Wiedmann M., Kuhn A., Phillips G.J., Dalbey R.E. Nature 406:637-641(2000) [PubMed: 10949305] [Abstract] Cited for: FUNCTION IN SEC-INDEPENDENT PATHWAY.
[8]	"SecDFyajC forms a heterotetrameric complex with YidC." Nouwen N., Driessen A.J.M. Mol. Microbiol. 44:1397-1405(2002) [PubMed: 12068816] [Abstract] Cited for: INTERACTION WITH SECD AND SECF.
[9]	"Targeting, insertion, and localization of Escherichia coli YidC." Urbanus M.L., Froederberg L., Drew D., Bjoerk P., de Gier J.-W., Brunner J., Oudega B., Luirink J. J. Biol. Chem. 277:12718-12723(2002) [PubMed: 11821429] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"A conserved function of YidC in the biogenesis of respiratory chain complexes." Van Der Laan M., Urbanus M.L., Ten Hagen-Jongman C.M., Nouwen N., Oudega B., Harms N., Driessen A.J.M., Luirink J. Proc. Natl. Acad. Sci. U.S.A. 100:5801-5806(2003) [PubMed: 12724529] [Abstract] Cited for: FUNCTION.
[11]	"Defining the regions of Escherichia coli YidC that contribute to activity." Jiang F., Chen M., Yi L., de Gier J.-W.L., Kuhn A., Dalbey R.E. J. Biol. Chem. 278:48965-48972(2003) [PubMed: 14506280] [Abstract] Cited for: MUTAGENESIS OF ILE-361 AND LEU-436.
[12]	"YidC is strictly required for membrane insertion of subunits a and c of the F(1)F(0)ATP synthase and SecE of the SecYEG translocase." Yi L., Jiang F., Chen M., Cain B., Bolhuis A., Dalbey R.E. Biochemistry 42:10537-10544(2003) [PubMed: 12950181] [Abstract] Cited for: FUNCTION.
[13]	"Protein complexes of the Escherichia coli cell envelope." Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O. J. Biol. Chem. 280:34409-34419(2005) [PubMed: 16079137] [Abstract] Cited for: SUBUNIT, SUBCELLULAR LOCATION. Strain: BL21-DE3.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L10328 Genomic DNA. Translation: AAA62056.1.
U00096 Genomic DNA. Translation: AAC76728.1.
AP009048 Genomic DNA. Translation: BAE77588.1.
M11056 Genomic DNA. No translation available.

PIR

B65173.

RefSeq

AP_004087.1.
NP_418161.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BLC	X-ray	2.50	A/B	26-340	[»]
3BS6	X-ray	1.80	A/B	56-329	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:12442N.

STRING

P25714.

Protein family/group databases

TCDB

2.A.9.3.1. cytochrome oxidase biogenesis (Oxa1) family.

Genome annotation databases

GeneID

948214.

GenomeReviews

Gene locus JW3683 in contig AP009048_GR.
Gene locus b3705 in contig U00096_GR.

KEGG

ecj:JW3683.
eco:b3705.

Organism-specific databases

EchoBASE

EB1183.

EcoGene

EG11197. oxaA.

CMR

Search...

Phylogenomic databases

HOGENOM

P25714.

OMA

NVEKEAF.

Enzyme and pathway databases

BioCyc

EcoCyc:YIDC.

Gene expression databases

Genevestigator

P25714.

Family and domain databases

HAMAP

MF_01810.
[Tree]

InterPro

IPR020001. Membrane_insert_OxaA/YidC_core.
IPR001708. Membrane_insertion_OxaA/YidC.
IPR013308. Membrane_insertion_YidC.
IPR019998. Membrane_insertion_YidC/OxoA_N.
[Graphical view]

PANTHER

PTHR12428. Innermemb_insert. 1 hit.

Pfam

PF02096. 60KD_IMP. 1 hit.
[Graphical view]

PRINTS

PR00701. 60KDINNERMP.
PR01900. YIDCPROTEIN.

TIGRFAMs

TIGR03593. YidC_nterm. 1 hit.
TIGR03592. YidC_oxa1_C. 1 hit.

ProtoNet

Search...

Entry information

Entry name

OXAA_ECOLI

Accession

Primary (citable) accession number: P25714
Secondary accession number(s): Q2M818

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	July 1, 1993
Last modified:	November 3, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)