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P25708 (NDUV1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-51kD
Short name=CI-51kD
NADH dehydrogenase flavoprotein 1
NADH-ubiquinone oxidoreductase 51 kDa subunit
Gene names
Name:NDUFV1
Synonyms:UQOR1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

NADH + acceptor = NAD+ + reduced acceptor.

Cofactor

Binds 1 FMN Potential.

Binds 1 4Fe-4S cluster Potential.

Subunit structure

Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Sequence similarities

Belongs to the complex I 51 kDa subunit family.

Sequence caution

The sequence AAA30661.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2020Mitochondrion
Chain21 – 464444NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
PRO_0000019975

Regions

Nucleotide binding87 – 9610NAD(H) By similarity
Nucleotide binding199 – 24749FMN By similarity

Sites

Metal binding3791Iron-sulfur (4Fe-4S) Potential
Metal binding3821Iron-sulfur (4Fe-4S) Potential
Metal binding3851Iron-sulfur (4Fe-4S) Potential
Metal binding4251Iron-sulfur (4Fe-4S) Potential

Amino acid modifications

Modified residue811N6-acetyllysine; alternate By similarity
Modified residue811N6-succinyllysine; alternate By similarity
Modified residue1041N6-acetyllysine By similarity
Modified residue3751N6-acetyllysine By similarity

Experimental info

Sequence conflict4131W → C in AAA30450. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P25708 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 8ACEC256E026B317

FASTA46450,652
        10         20         30         40         50         60 
MLAARRLLGG SLPARVSVRF SGDTTAPKKT SFGSLKDEDR IFTNLYGRHD WRLKGAQSRG 

        70         80         90        100        110        120 
DWYKTKEILL KGPDWILGEV KTSGLRGRGG AGFPTGLKWS FMNKPSDGRP KYLVVNADEG 

       130        140        150        160        170        180 
EPGTCKDREI IRHDPHKLVE GCLVGGRAMG ARAAYIYIRG EFYNEASNLQ VAIREAYEAG 

       190        200        210        220        230        240 
LIGKNACGSG YDFDVFVVRG AGAYICGEET ALIESIEGKQ GKPRLKPPFP ADVGVFGCPT 

       250        260        270        280        290        300 
TVANVETVAV SPTICRRGGA WFASFGRERN SGTKLFNISG HVNNPCTVEE EMSVPLKELI 

       310        320        330        340        350        360 
EKHAGGVTGG WDNLLAVIPG GSSTPLIPKS VCETVLMDFD ALIQAQTGLG TAAVIVMDRS 

       370        380        390        400        410        420 
TDIVKAIARL IEFYKHESCG QCTPCREGVD WMNKVMARFV RGDARPAEID SLWEISKQIE 

       430        440        450        460 
GHTICALGDG AAWPVQGLIR HFRPELEERM QQFAQQHQAR QAAF 

« Hide

References

« Hide 'large scale' references
[1]"cDNA-derived amino acid sequence of the NADH-binding 51-kDa subunit of the bovine respiratory NADH dehydrogenase reveals striking similarities to a bacterial NAD(+)-reducing hydrogenase."
Patel S.D., Aebersold R., Attardi G.
Proc. Natl. Acad. Sci. U.S.A. 88:4225-4229(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"Relationship between mitochondrial NADH-ubiquinone reductase and a bacterial NAD-reducing hydrogenase."
Pilkington S.J., Skehel J.M., Gennis R.B., Walker J.E.
Biochemistry 30:2166-2175(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Basal ganglia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M58607 mRNA. Translation: AAA30450.1.
M63009 mRNA. Translation: AAA30661.1. Different initiation.
BC118304 mRNA. Translation: AAI18305.1.
PIRA39362.
RefSeqNP_777233.1. NM_174808.1.
UniGeneBt.4072.

3D structure databases

ProteinModelPortalP25708.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP25708. 3 interactions.
STRING9913.ENSBTAP00000029026.

Chemistry

ChEMBLCHEMBL614865.

Protein family/group databases

TCDB3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbP25708.
PRIDEP25708.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000029026; ENSBTAP00000029026; ENSBTAG00000021776.
GeneID287014.
KEGGbta:287014.

Organism-specific databases

CTD4723.

Phylogenomic databases

eggNOGCOG1894.
GeneTreeENSGT00390000010641.
HOGENOMHOG000251534.
HOVERGENHBG006542.
InParanoidP25708.
KOK03942.
OMAIRHYRPE.
OrthoDBEOG7327NS.
TreeFamTF300381.

Family and domain databases

InterProIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. NADH_UbQ_OxRdtase_51kDa_su.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsTIGR01959. nuoF_fam. 1 hit.
PROSITEPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20806538.

Entry information

Entry nameNDUV1_BOVIN
AccessionPrimary (citable) accession number: P25708
Secondary accession number(s): Q148I2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families