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P25708

- NDUV1_BOVIN

UniProt

P25708 - NDUV1_BOVIN

Protein

NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Gene

NDUFV1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 FMN.Curated
    Binds 1 4Fe-4S cluster.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi379 – 3791Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi382 – 3821Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi385 – 3851Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi425 – 4251Iron-sulfur (4Fe-4S)Sequence Analysis

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi87 – 9610NAD(H)By similarity
    Nucleotide bindingi199 – 24749FMNBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. FMN binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. NAD binding Source: InterPro
    5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    ReactomeiREACT_223064. Respiratory electron transport.

    Protein family/group databases

    TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-51kD
    Short name:
    CI-51kD
    NADH dehydrogenase flavoprotein 1
    NADH-ubiquinone oxidoreductase 51 kDa subunit
    Gene namesi
    Name:NDUFV1
    Synonyms:UQOR1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 29

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: Ensembl

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2020MitochondrionAdd
    BLAST
    Chaini21 – 464444NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrialPRO_0000019975Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei81 – 811N6-acetyllysine; alternateBy similarity
    Modified residuei81 – 811N6-succinyllysine; alternateBy similarity
    Modified residuei104 – 1041N6-acetyllysineBy similarity
    Modified residuei375 – 3751N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP25708.
    PRIDEiP25708.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

    Protein-protein interaction databases

    IntActiP25708. 3 interactions.
    STRINGi9913.ENSBTAP00000029026.

    Structurei

    3D structure databases

    ProteinModelPortaliP25708.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 51 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1894.
    GeneTreeiENSGT00390000010641.
    HOGENOMiHOG000251534.
    HOVERGENiHBG006542.
    InParanoidiP25708.
    KOiK03942.
    OMAiIRHYRPE.
    OrthoDBiEOG7327NS.
    TreeFamiTF300381.

    Family and domain databases

    InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
    IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd.
    IPR011537. NADH-UbQ_OxRdtase_suF.
    IPR011538. NADH_UbQ_OxRdtase_51kDa_su.
    IPR019554. Soluble_ligand-bd.
    [Graphical view]
    PfamiPF01512. Complex1_51K. 1 hit.
    PF10589. NADH_4Fe-4S. 1 hit.
    PF10531. SLBB. 1 hit.
    [Graphical view]
    SMARTiSM00928. NADH_4Fe-4S. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
    PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
    PS00645. COMPLEX1_51K_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25708-1 [UniParc]FASTAAdd to Basket

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    MLAARRLLGG SLPARVSVRF SGDTTAPKKT SFGSLKDEDR IFTNLYGRHD    50
    WRLKGAQSRG DWYKTKEILL KGPDWILGEV KTSGLRGRGG AGFPTGLKWS 100
    FMNKPSDGRP KYLVVNADEG EPGTCKDREI IRHDPHKLVE GCLVGGRAMG 150
    ARAAYIYIRG EFYNEASNLQ VAIREAYEAG LIGKNACGSG YDFDVFVVRG 200
    AGAYICGEET ALIESIEGKQ GKPRLKPPFP ADVGVFGCPT TVANVETVAV 250
    SPTICRRGGA WFASFGRERN SGTKLFNISG HVNNPCTVEE EMSVPLKELI 300
    EKHAGGVTGG WDNLLAVIPG GSSTPLIPKS VCETVLMDFD ALIQAQTGLG 350
    TAAVIVMDRS TDIVKAIARL IEFYKHESCG QCTPCREGVD WMNKVMARFV 400
    RGDARPAEID SLWEISKQIE GHTICALGDG AAWPVQGLIR HFRPELEERM 450
    QQFAQQHQAR QAAF 464
    Length:464
    Mass (Da):50,652
    Last modified:June 1, 1994 - v2
    Checksum:i8ACEC256E026B317
    GO

    Sequence cautioni

    The sequence AAA30661.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti413 – 4131W → C in AAA30450. (PubMed:1900194)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58607 mRNA. Translation: AAA30450.1.
    M63009 mRNA. Translation: AAA30661.1. Different initiation.
    BC118304 mRNA. Translation: AAI18305.1.
    PIRiA39362.
    RefSeqiNP_777233.1. NM_174808.1.
    UniGeneiBt.4072.

    Genome annotation databases

    EnsembliENSBTAT00000029026; ENSBTAP00000029026; ENSBTAG00000021776.
    GeneIDi287014.
    KEGGibta:287014.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58607 mRNA. Translation: AAA30450.1 .
    M63009 mRNA. Translation: AAA30661.1 . Different initiation.
    BC118304 mRNA. Translation: AAI18305.1 .
    PIRi A39362.
    RefSeqi NP_777233.1. NM_174808.1.
    UniGenei Bt.4072.

    3D structure databases

    ProteinModelPortali P25708.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P25708. 3 interactions.
    STRINGi 9913.ENSBTAP00000029026.

    Chemistry

    ChEMBLi CHEMBL614865.

    Protein family/group databases

    TCDBi 3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PaxDbi P25708.
    PRIDEi P25708.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000029026 ; ENSBTAP00000029026 ; ENSBTAG00000021776 .
    GeneIDi 287014.
    KEGGi bta:287014.

    Organism-specific databases

    CTDi 4723.

    Phylogenomic databases

    eggNOGi COG1894.
    GeneTreei ENSGT00390000010641.
    HOGENOMi HOG000251534.
    HOVERGENi HBG006542.
    InParanoidi P25708.
    KOi K03942.
    OMAi IRHYRPE.
    OrthoDBi EOG7327NS.
    TreeFami TF300381.

    Enzyme and pathway databases

    Reactomei REACT_223064. Respiratory electron transport.

    Miscellaneous databases

    NextBioi 20806538.

    Family and domain databases

    InterProi IPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
    IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd.
    IPR011537. NADH-UbQ_OxRdtase_suF.
    IPR011538. NADH_UbQ_OxRdtase_51kDa_su.
    IPR019554. Soluble_ligand-bd.
    [Graphical view ]
    Pfami PF01512. Complex1_51K. 1 hit.
    PF10589. NADH_4Fe-4S. 1 hit.
    PF10531. SLBB. 1 hit.
    [Graphical view ]
    SMARTi SM00928. NADH_4Fe-4S. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01959. nuoF_fam. 1 hit.
    PROSITEi PS00644. COMPLEX1_51K_1. 1 hit.
    PS00645. COMPLEX1_51K_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA-derived amino acid sequence of the NADH-binding 51-kDa subunit of the bovine respiratory NADH dehydrogenase reveals striking similarities to a bacterial NAD(+)-reducing hydrogenase."
      Patel S.D., Aebersold R., Attardi G.
      Proc. Natl. Acad. Sci. U.S.A. 88:4225-4229(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "Relationship between mitochondrial NADH-ubiquinone reductase and a bacterial NAD-reducing hydrogenase."
      Pilkington S.J., Skehel J.M., Gennis R.B., Walker J.E.
      Biochemistry 30:2166-2175(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Basal ganglia.

    Entry informationi

    Entry nameiNDUV1_BOVIN
    AccessioniPrimary (citable) accession number: P25708
    Secondary accession number(s): Q148I2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3