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Protein

NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Gene

NDUFV1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • FMNCuratedNote: Binds 1 FMN.Curated
  • [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi379Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi382Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi385Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi425Iron-sulfur (4Fe-4S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi87 – 96NAD(H)By similarity10
Nucleotide bindingi199 – 247FMNBy similarityAdd BLAST49

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-51kD
Short name:
CI-51kD
NADH dehydrogenase flavoprotein 1
NADH-ubiquinone oxidoreductase 51 kDa subunit
Gene namesi
Name:NDUFV1
Synonyms:UQOR1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 29

Subcellular locationi

GO - Cellular componenti

  • mitochondrial respiratory chain complex I Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL614865.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 20MitochondrionAdd BLAST20
ChainiPRO_000001997521 – 464NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrialAdd BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81N6-acetyllysine; alternateBy similarity1
Modified residuei81N6-succinyllysine; alternateBy similarity1
Modified residuei104N6-acetyllysineBy similarity1
Modified residuei257Omega-N-methylarginineBy similarity1
Modified residuei375N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PaxDbiP25708.
PeptideAtlasiP25708.
PRIDEiP25708.

Expressioni

Gene expression databases

BgeeiENSBTAG00000021776.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.2 Publications

Protein-protein interaction databases

IntActiP25708. 3 interactors.
STRINGi9913.ENSBTAP00000029026.

Chemistry databases

BindingDBiP25708.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35F1-464[»]
5LDWelectron microscopy4.27F21-464[»]
5LDXelectron microscopy5.60F21-464[»]
ProteinModelPortaliP25708.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2658. Eukaryota.
COG1894. LUCA.
GeneTreeiENSGT00390000010641.
HOGENOMiHOG000251534.
HOVERGENiHBG006542.
InParanoidiP25708.
KOiK03942.
OMAiGTTWMMN.
OrthoDBiEOG091G05AF.
TreeFamiTF300381.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25708-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAARRLLGG SLPARVSVRF SGDTTAPKKT SFGSLKDEDR IFTNLYGRHD
60 70 80 90 100
WRLKGAQSRG DWYKTKEILL KGPDWILGEV KTSGLRGRGG AGFPTGLKWS
110 120 130 140 150
FMNKPSDGRP KYLVVNADEG EPGTCKDREI IRHDPHKLVE GCLVGGRAMG
160 170 180 190 200
ARAAYIYIRG EFYNEASNLQ VAIREAYEAG LIGKNACGSG YDFDVFVVRG
210 220 230 240 250
AGAYICGEET ALIESIEGKQ GKPRLKPPFP ADVGVFGCPT TVANVETVAV
260 270 280 290 300
SPTICRRGGA WFASFGRERN SGTKLFNISG HVNNPCTVEE EMSVPLKELI
310 320 330 340 350
EKHAGGVTGG WDNLLAVIPG GSSTPLIPKS VCETVLMDFD ALIQAQTGLG
360 370 380 390 400
TAAVIVMDRS TDIVKAIARL IEFYKHESCG QCTPCREGVD WMNKVMARFV
410 420 430 440 450
RGDARPAEID SLWEISKQIE GHTICALGDG AAWPVQGLIR HFRPELEERM
460
QQFAQQHQAR QAAF
Length:464
Mass (Da):50,652
Last modified:June 1, 1994 - v2
Checksum:i8ACEC256E026B317
GO

Sequence cautioni

The sequence AAA30661 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti413W → C in AAA30450 (PubMed:1900194).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58607 mRNA. Translation: AAA30450.1.
M63009 mRNA. Translation: AAA30661.1. Different initiation.
BC118304 mRNA. Translation: AAI18305.1.
PIRiA39362.
RefSeqiNP_777233.1. NM_174808.1.
UniGeneiBt.4072.

Genome annotation databases

EnsembliENSBTAT00000029026; ENSBTAP00000029026; ENSBTAG00000021776.
GeneIDi287014.
KEGGibta:287014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58607 mRNA. Translation: AAA30450.1.
M63009 mRNA. Translation: AAA30661.1. Different initiation.
BC118304 mRNA. Translation: AAI18305.1.
PIRiA39362.
RefSeqiNP_777233.1. NM_174808.1.
UniGeneiBt.4072.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LC5electron microscopy4.35F1-464[»]
5LDWelectron microscopy4.27F21-464[»]
5LDXelectron microscopy5.60F21-464[»]
ProteinModelPortaliP25708.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP25708. 3 interactors.
STRINGi9913.ENSBTAP00000029026.

Chemistry databases

BindingDBiP25708.
ChEMBLiCHEMBL614865.

Protein family/group databases

TCDBi3.D.1.6.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP25708.
PeptideAtlasiP25708.
PRIDEiP25708.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000029026; ENSBTAP00000029026; ENSBTAG00000021776.
GeneIDi287014.
KEGGibta:287014.

Organism-specific databases

CTDi4723.

Phylogenomic databases

eggNOGiKOG2658. Eukaryota.
COG1894. LUCA.
GeneTreeiENSGT00390000010641.
HOGENOMiHOG000251534.
HOVERGENiHBG006542.
InParanoidiP25708.
KOiK03942.
OMAiGTTWMMN.
OrthoDBiEOG091G05AF.
TreeFamiTF300381.

Enzyme and pathway databases

ReactomeiR-BTA-611105. Respiratory electron transport.
R-BTA-6799198. Complex I biogenesis.

Miscellaneous databases

PROiP25708.

Gene expression databases

BgeeiENSBTAG00000021776.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDUV1_BOVIN
AccessioniPrimary (citable) accession number: P25708
Secondary accession number(s): Q148I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.