P25697 (KPPR_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribulokinase, chloroplastic
Short name=PRK
Short name=PRKase
EC=2.7.1.19

Alternative name(s):
Phosphopentokinase

Gene names

Ordered Locus Names:	At1g32060
ORF Names:	T12O21.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-bisphosphate.
Enzyme regulation	Light regulated via thioredoxin by reversible oxidation/reduction of sulfhydryl/disulfide groups.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the phosphoribulokinase family.

Ontologies

Keywords
Biological process	Calvin cycle Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from expression pattern PubMed 17028151. Source: TAIR reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway response to cold Inferred from expression pattern PubMed 16923014. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR chloroplast envelope Inferred from direct assay PubMed 12938931. Source: TAIR chloroplast stroma Inferred from direct assay PubMed 16207701PubMed 18633119PubMed 20061580. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay PubMed 15322131. Source: TAIR stromule Inferred from direct assay PubMed 16923014. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribulokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449190,EBI-449157

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Chloroplast
	Chain	47 – 395	349	Phosphoribulokinase, chloroplastic		PRO_0000025751
Amino acid modifications
	Disulfide bond	61 ↔ 100		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25697 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 4660A92EF7E39BC6	FASTA	395	44,464
	10 20 30 40 50 60 MAVSTIYSTQ ALNSTHFLTS SSSSKQVFLY RRQPQTNRRF NTLITCAQET IVIGLAADSG 70 80 90 100 110 120 CGKSTFMRRL TSVFGGAAKP PKGGNPDSNT LISDTTTVIC LDDYHSLDRY GRKEQKVTAL 130 140 150 160 170 180 DPRANDFDLM YEQVKALKNG IAVEKPIYNH VTGLLDPPEL IQPPKILVIE GLHPMFDERV 190 200 210 220 230 240 RDLLDFSIYL DISNEVKFAW KIQRDMAERG HSLESIKASI EARKPDFDAF IDPQKQYADA 250 260 270 280 290 300 VIEVLPTTLI PDDNEGKVLR VRLIMKEGVK YFSPVYLFDE GSTISWIPCG RKLTCSYPGI 310 320 330 340 350 360 KFNYEPDSYF DHEVSVLEMD GQFDRLDELI YVESHLSNLS TKFYGEVTQQ MLKHADFPGS 370 380 390 NNGTGLFQTI VGLKIRDLYE QLIANKATAR AEAKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a cDNA clone encoding chloroplast phosphoribulokinase from Arabidopsis thaliana." Horsnell P.R., Raines C.A. Plant Mol. Biol. 17:183-184(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. C24.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58149 mRNA. Translation: CAA41155.1. AC074309 Genomic DNA. Translation: AAG50797.1. CP002684 Genomic DNA. Translation: AEE31430.1. AY044335 mRNA. Translation: AAK73276.1. AY128355 mRNA. Translation: AAM91558.1. BT000019 mRNA. Translation: AAN15338.1. AY084576 mRNA. Translation: AAM61142.1.
IPI	IPI00516350.
PIR	S16583.
RefSeq	NP_174486.1. NM_102940.5.
UniGene	At.22659.
3D structure databases
ProteinModelPortal	P25697.
SMR	P25697. Positions 49-242.
ModBase	Search...
Protein-protein interaction databases
IntAct	P25697. 2 interactions.
STRING	3702.AT1G32060.1-P.
2D gel databases
SWISS-2DPAGE	P25697.
World-2DPAGE	0003:P25697.
Proteomic databases
PaxDb	P25697.
PRIDE	P25697.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G32060.1; AT1G32060.1; AT1G32060.
GeneID	840098.
KEGG	ath:AT1G32060.
Organism-specific databases
TAIR	At1g32060.
Phylogenomic databases
eggNOG	COG0572.
HOGENOM	HOG000230117.
InParanoid	P25697.
KO	K00855.
OMA	VKIAWKI.
PhylomeDB	P25697.
ProtClustDB	PLN02348.
Enzyme and pathway databases
BioCyc	ARA:AT1G32060-MONOMER. MetaCyc:AT1G32060-MONOMER.
UniPathway	UPA00116.
Gene expression databases
Genevestigator	P25697.
GermOnline	AT1G32060. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006082. PRK. IPR006083. PRK/URK. [Graphical view]
Pfam	PF00485. PRK. 1 hit. [Graphical view]
PRINTS	PR00478. PHRIBLKINASE.
PROSITE	PS00567. PHOSPHORIBULOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPPR_ARATH

Accession

Primary (citable) accession number: P25697

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program