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Protein

Phosphoribulokinase, chloroplastic

Gene

At1g32060

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-bisphosphate.

Enzyme regulationi

Light regulated via thioredoxin by reversible oxidation/reduction of sulfhydryl/disulfide groups.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • disordered domain specific binding Source: CAFA
  • phosphoribulokinase activity Source: CAFA
  • protein homodimerization activity Source: CAFA
  • uridine kinase activity Source: GO_Central

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • pyrimidine nucleobase metabolic process Source: GO_Central
  • pyrimidine nucleoside salvage Source: GO_Central
  • reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
  • response to cold Source: TAIR
  • response to cytokinin Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
Biological processCalvin cycle, Photosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:AT1G32060-MONOMER.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribulokinase, chloroplastic (EC:2.7.1.19)
Short name:
PRK
Short name:
PRKase
Alternative name(s):
Phosphopentokinase
Gene namesi
Ordered Locus Names:At1g32060
ORF Names:T12O21.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G32060.
TAIRilocus:2195366. AT1G32060.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 46ChloroplastAdd BLAST46
ChainiPRO_000002575147 – 395Phosphoribulokinase, chloroplasticAdd BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi61 ↔ 100By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP25697.
PRIDEiP25697.

2D gel databases

SWISS-2DPAGEiP25697.
World-2DPAGEi0003:P25697.

PTM databases

iPTMnetiP25697.

Expressioni

Gene expression databases

ExpressionAtlasiP25697. baseline and differential.
GenevisibleiP25697. AT.

Interactioni

GO - Molecular functioni

  • disordered domain specific binding Source: CAFA
  • protein homodimerization activity Source: CAFA

Protein-protein interaction databases

BioGridi25332. 3 interactors.
IntActiP25697. 2 interactors.
STRINGi3702.AT1G32060.1.

Structurei

3D structure databases

ProteinModelPortaliP25697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoribulokinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IMEH. Eukaryota.
COG0572. LUCA.
HOGENOMiHOG000230117.
InParanoidiP25697.
KOiK00855.
OMAiMKPIYNH.
OrthoDBiEOG09360B0Z.
PhylomeDBiP25697.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR006082. PRK.
IPR006083. PRK/URK.
PfamiView protein in Pfam
PF00485. PRK. 1 hit.
PRINTSiPR00478. PHRIBLKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00567. PHOSPHORIBULOKINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSTIYSTQ ALNSTHFLTS SSSSKQVFLY RRQPQTNRRF NTLITCAQET
60 70 80 90 100
IVIGLAADSG CGKSTFMRRL TSVFGGAAKP PKGGNPDSNT LISDTTTVIC
110 120 130 140 150
LDDYHSLDRY GRKEQKVTAL DPRANDFDLM YEQVKALKNG IAVEKPIYNH
160 170 180 190 200
VTGLLDPPEL IQPPKILVIE GLHPMFDERV RDLLDFSIYL DISNEVKFAW
210 220 230 240 250
KIQRDMAERG HSLESIKASI EARKPDFDAF IDPQKQYADA VIEVLPTTLI
260 270 280 290 300
PDDNEGKVLR VRLIMKEGVK YFSPVYLFDE GSTISWIPCG RKLTCSYPGI
310 320 330 340 350
KFNYEPDSYF DHEVSVLEMD GQFDRLDELI YVESHLSNLS TKFYGEVTQQ
360 370 380 390
MLKHADFPGS NNGTGLFQTI VGLKIRDLYE QLIANKATAR AEAKA
Length:395
Mass (Da):44,464
Last modified:May 1, 1992 - v1
Checksum:i4660A92EF7E39BC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58149 mRNA. Translation: CAA41155.1.
AC074309 Genomic DNA. Translation: AAG50797.1.
CP002684 Genomic DNA. Translation: AEE31430.1.
AY044335 mRNA. Translation: AAK73276.1.
AY128355 mRNA. Translation: AAM91558.1.
BT000019 mRNA. Translation: AAN15338.1.
AY084576 mRNA. Translation: AAM61142.1.
PIRiS16583.
RefSeqiNP_174486.1. NM_102940.6.
UniGeneiAt.22659.

Genome annotation databases

EnsemblPlantsiAT1G32060.1; AT1G32060.1; AT1G32060.
GeneIDi840098.
GrameneiAT1G32060.1; AT1G32060.1; AT1G32060.
KEGGiath:AT1G32060.

Similar proteinsi

Entry informationi

Entry nameiKPPR_ARATH
AccessioniPrimary (citable) accession number: P25697
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: August 30, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families