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Protein

Bifunctional enolase 2/transcriptional activator

Gene

ENO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional enzyme that acts as an enolase involved in the metabolism and as a positive regulator of cold-responsive gene transcription. Binds to the cis-element the gene promoter of STZ/ZAT10, a zinc finger transcriptional repressor.2 Publications

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+Note: Mg2+ is required for catalysis and for stabilizing the dimer.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
  2. no protein annotated in this organism
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
  4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
  5. Pyruvate kinase (At5g08570), Pyruvate kinase (At5g56350), Pyruvate kinase (F1I16_60), Pyruvate kinase (At5g63680), Pyruvate kinase (At3g25960), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (At3g52990), Pyruvate kinase (At2g36580), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (T11I18.16), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei163 – 1631SubstrateBy similarity
Binding sitei172 – 1721SubstrateBy similarity
Active sitei215 – 2151Proton donorBy similarity
Metal bindingi250 – 2501MagnesiumBy similarity
Metal bindingi300 – 3001MagnesiumBy similarity
Binding sitei300 – 3001SubstrateBy similarity
Metal bindingi327 – 3271MagnesiumBy similarity
Binding sitei327 – 3271SubstrateBy similarity
Active sitei352 – 3521Proton acceptorBy similarity
Binding sitei403 – 4031SubstrateBy similarity

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • DNA binding Source: UniProtKB
  • magnesium ion binding Source: InterPro
  • phosphopyruvate hydratase activity Source: TAIR

GO - Biological processi

  • glycolytic process Source: UniProtKB-UniPathway
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to abscisic acid Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • response to light stimulus Source: TAIR
  • response to salt stress Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Repressor

Keywords - Biological processi

Glycolysis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G36530-MONOMER.
MetaCyc:AT2G36530-MONOMER.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enolase 2/transcriptional activator (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 2
2-phosphoglycerate dehydratase 2
LOW EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1
Gene namesi
Name:ENO2
Synonyms:LOS2
Ordered Locus Names:At2g36530
ORF Names:F1O11.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G36530.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • membrane Source: TAIR
  • mitochondrial envelope Source: TAIR
  • mitochondrion Source: TAIR
  • nucleus Source: TAIR
  • phosphopyruvate hydratase complex Source: InterPro
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi326 – 3261G → S: Impairs cold-responsive gene transcription. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Bifunctional enolase 2/transcriptional activatorPRO_0000134067Add
BLAST

Proteomic databases

PaxDbiP25696.
PRIDEiP25696.

2D gel databases

SWISS-2DPAGEP25696.

PTM databases

iPTMnetiP25696.
SwissPalmiP25696.

Expressioni

Gene expression databases

GenevisibleiP25696. AT.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi3570. 5 interactions.
STRINGi3702.AT2G36530.1.

Structurei

3D structure databases

ProteinModelPortaliP25696.
SMRiP25696. Positions 3-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni379 – 3824Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
HOGENOMiHOG000072174.
InParanoidiP25696.
KOiK01689.
OMAiFAPNIMS.
OrthoDBiEOG093608UK.
PhylomeDBiP25696.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATITVVKAR QIFDSRGNPT VEVDIHTSNG IKVTAAVPSG ASTGIYEALE
60 70 80 90 100
LRDGGSDYLG KGVSKAVGNV NNIIGPALIG KDPTQQTAID NFMVHELDGT
110 120 130 140 150
QNEWGWCKQK LGANAILAVS LAVCKAGAVV SGIPLYKHIA NLAGNPKIVL
160 170 180 190 200
PVPAFNVING GSHAGNKLAM QEFMILPVGA ASFKEAMKMG VEVYHHLKSV
210 220 230 240 250
IKKKYGQDAT NVGDEGGFAP NIQENKEGLE LLKTAIEKAG YTGKVVIGMD
260 270 280 290 300
VAASEFYSED KTYDLNFKEE NNNGSQKISG DALKDLYKSF VAEYPIVSIE
310 320 330 340 350
DPFDQDDWEH YAKMTTECGT EVQIVGDDLL VTNPKRVAKA IAEKSCNALL
360 370 380 390 400
LKVNQIGSVT ESIEAVKMSK KAGWGVMTSH RSGETEDTFI ADLAVGLSTG
410 420 430 440
QIKTGAPCRS ERLAKYNQLL RIEEELGSEA IYAGVNFRKP VEPY
Length:444
Mass (Da):47,719
Last modified:May 1, 1992 - v1
Checksum:i4037C7E0390B6C2B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731I → V in AAL06912 (PubMed:14593172).Curated
Sequence conflicti160 – 1601G → D in AAM12985 (PubMed:14593172).Curated
Sequence conflicti207 – 2071Q → H in AAL59917 (PubMed:14593172).Curated
Sequence conflicti386 – 3861E → K in AAL06912 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58107 Genomic DNA. Translation: CAA41114.1.
AC006919 Genomic DNA. Translation: AAD24635.1.
CP002685 Genomic DNA. Translation: AEC09265.1.
AF424603 mRNA. Translation: AAL11597.1.
AY054253 mRNA. Translation: AAL06912.1.
AY072095 mRNA. Translation: AAL59917.1.
AY092986 mRNA. Translation: AAM12985.1.
AY150418 mRNA. Translation: AAN12963.1.
PIRiJQ1187.
RefSeqiNP_181192.1. NM_129209.3.
UniGeneiAt.24124.
At.25354.
At.74012.

Genome annotation databases

EnsemblPlantsiAT2G36530.1; AT2G36530.1; AT2G36530.
GeneIDi818226.
GrameneiAT2G36530.1; AT2G36530.1; AT2G36530.
KEGGiath:AT2G36530.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58107 Genomic DNA. Translation: CAA41114.1.
AC006919 Genomic DNA. Translation: AAD24635.1.
CP002685 Genomic DNA. Translation: AEC09265.1.
AF424603 mRNA. Translation: AAL11597.1.
AY054253 mRNA. Translation: AAL06912.1.
AY072095 mRNA. Translation: AAL59917.1.
AY092986 mRNA. Translation: AAM12985.1.
AY150418 mRNA. Translation: AAN12963.1.
PIRiJQ1187.
RefSeqiNP_181192.1. NM_129209.3.
UniGeneiAt.24124.
At.25354.
At.74012.

3D structure databases

ProteinModelPortaliP25696.
SMRiP25696. Positions 3-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3570. 5 interactions.
STRINGi3702.AT2G36530.1.

PTM databases

iPTMnetiP25696.
SwissPalmiP25696.

2D gel databases

SWISS-2DPAGEP25696.

Proteomic databases

PaxDbiP25696.
PRIDEiP25696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G36530.1; AT2G36530.1; AT2G36530.
GeneIDi818226.
GrameneiAT2G36530.1; AT2G36530.1; AT2G36530.
KEGGiath:AT2G36530.

Organism-specific databases

TAIRiAT2G36530.

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
HOGENOMiHOG000072174.
InParanoidiP25696.
KOiK01689.
OMAiFAPNIMS.
OrthoDBiEOG093608UK.
PhylomeDBiP25696.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
BioCyciARA:AT2G36530-MONOMER.
MetaCyc:AT2G36530-MONOMER.

Miscellaneous databases

PROiP25696.

Gene expression databases

GenevisibleiP25696. AT.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENO2_ARATH
AccessioniPrimary (citable) accession number: P25696
Secondary accession number(s): Q8RWM8, Q8VYG4, Q940N0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 7, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.