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Protein

Bifunctional enolase 2/transcriptional activator

Gene

ENO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multifunctional enzyme that acts as an enolase involved in the metabolism and as a positive regulator of cold-responsive gene transcription. Binds to the cis-element the gene promoter of STZ/ZAT10, a zinc finger transcriptional repressor.2 Publications

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+Note: Mg2+ is required for catalysis and for stabilizing the dimer.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
  2. Phosphoglycerate kinase 3, cytosolic (PGK3)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
  4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
  5. Pyruvate kinase (AXX17_At5g51800), Pyruvate kinase (AXX17_At3g24740), Pyruvate kinase (AXX17_At5g63250), Pyruvate kinase (AXX17_At3g50280), Pyruvate kinase (AXX17_ATUG04870), Pyruvate kinase (MAH20.13), Pyruvate kinase (MCD7.8), Pyruvate kinase (MBK5.16), Pyruvate kinase (F1I16_60), Pyruvate kinase (At3g25960), Pyruvate kinase (AXX17_At3g50440), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At5g08490), Pyruvate kinase (AXX17_At5g55530), Pyruvate kinase (AXX17_At3g28070), Pyruvate kinase (At2g36580), Pyruvate kinase (AXX17_At3g47400), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At3g03460), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (AXX17_At2g33300), Pyruvate kinase (At3g04050), Pyruvate kinase (AXX17_At4g30430), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (AXX17_At1g33230), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei163SubstrateBy similarity1
Binding sitei172SubstrateBy similarity1
Active sitei215Proton donorBy similarity1
Metal bindingi250MagnesiumBy similarity1
Metal bindingi300MagnesiumBy similarity1
Binding sitei300SubstrateBy similarity1
Metal bindingi327MagnesiumBy similarity1
Binding sitei327SubstrateBy similarity1
Active sitei352Proton acceptorBy similarity1
Binding sitei403SubstrateBy similarity1

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • DNA binding Source: UniProtKB
  • magnesium ion binding Source: InterPro
  • phosphopyruvate hydratase activity Source: TAIR

GO - Biological processi

  • glycolytic process Source: UniProtKB-UniPathway
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to abscisic acid Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • response to light stimulus Source: TAIR
  • response to salt stress Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Lyase, Repressor
Biological processGlycolysis, Transcription, Transcription regulation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G36530-MONOMER
MetaCyc:AT2G36530-MONOMER
ReactomeiR-ATH-70171 Glycolysis
R-ATH-70263 Gluconeogenesis
UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enolase 2/transcriptional activator (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 2
2-phosphoglycerate dehydratase 2
LOW EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1
Gene namesi
Name:ENO2
Synonyms:LOS2
Ordered Locus Names:At2g36530
ORF Names:F1O11.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G36530
TAIRilocus:2044851 AT2G36530

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi326G → S: Impairs cold-responsive gene transcription. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340671 – 444Bifunctional enolase 2/transcriptional activatorAdd BLAST444

Proteomic databases

PaxDbiP25696
PRIDEiP25696

2D gel databases

SWISS-2DPAGEiP25696

PTM databases

iPTMnetiP25696
SwissPalmiP25696

Expressioni

Gene expression databases

ExpressionAtlasiP25696 differential
GenevisibleiP25696 AT

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi3570, 5 interactors
STRINGi3702.AT2G36530.1

Structurei

3D structure databases

ProteinModelPortaliP25696
SMRiP25696
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni379 – 382Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670 Eukaryota
COG0148 LUCA
HOGENOMiHOG000072174
InParanoidiP25696
KOiK01689
OMAiMTTECGD
OrthoDBiEOG093608UK
PhylomeDBiP25696

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

P25696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATITVVKAR QIFDSRGNPT VEVDIHTSNG IKVTAAVPSG ASTGIYEALE
60 70 80 90 100
LRDGGSDYLG KGVSKAVGNV NNIIGPALIG KDPTQQTAID NFMVHELDGT
110 120 130 140 150
QNEWGWCKQK LGANAILAVS LAVCKAGAVV SGIPLYKHIA NLAGNPKIVL
160 170 180 190 200
PVPAFNVING GSHAGNKLAM QEFMILPVGA ASFKEAMKMG VEVYHHLKSV
210 220 230 240 250
IKKKYGQDAT NVGDEGGFAP NIQENKEGLE LLKTAIEKAG YTGKVVIGMD
260 270 280 290 300
VAASEFYSED KTYDLNFKEE NNNGSQKISG DALKDLYKSF VAEYPIVSIE
310 320 330 340 350
DPFDQDDWEH YAKMTTECGT EVQIVGDDLL VTNPKRVAKA IAEKSCNALL
360 370 380 390 400
LKVNQIGSVT ESIEAVKMSK KAGWGVMTSH RSGETEDTFI ADLAVGLSTG
410 420 430 440
QIKTGAPCRS ERLAKYNQLL RIEEELGSEA IYAGVNFRKP VEPY
Length:444
Mass (Da):47,719
Last modified:May 1, 1992 - v1
Checksum:i4037C7E0390B6C2B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73I → V in AAL06912 (PubMed:14593172).Curated1
Sequence conflicti160G → D in AAM12985 (PubMed:14593172).Curated1
Sequence conflicti207Q → H in AAL59917 (PubMed:14593172).Curated1
Sequence conflicti386E → K in AAL06912 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58107 Genomic DNA Translation: CAA41114.1
AC006919 Genomic DNA Translation: AAD24635.1
CP002685 Genomic DNA Translation: AEC09265.1
AF424603 mRNA Translation: AAL11597.1
AY054253 mRNA Translation: AAL06912.1
AY072095 mRNA Translation: AAL59917.1
AY092986 mRNA Translation: AAM12985.1
AY150418 mRNA Translation: AAN12963.1
PIRiJQ1187
RefSeqiNP_181192.1, NM_129209.4
UniGeneiAt.24124
At.25354
At.74012

Genome annotation databases

EnsemblPlantsiAT2G36530.1; AT2G36530.1; AT2G36530
GeneIDi818226
GrameneiAT2G36530.1; AT2G36530.1; AT2G36530
KEGGiath:AT2G36530

Similar proteinsi

Entry informationi

Entry nameiENO2_ARATH
AccessioniPrimary (citable) accession number: P25696
Secondary accession number(s): Q8RWM8, Q8VYG4, Q940N0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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