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Protein

PHO85 cyclin-2

Gene

PCL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G1/S-specific cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Essential for the control of the cell cycle at the G1/S (start) transition. Together with cyclin PCL1, positively controls degradation of sphingoid long chain base kinase LCB4. The PCL2-PHO85 cyclin-CDK holoenzyme phosphorylates LCB4, which is required for its ubiquitination and degradation. PCL2-PHO85 also phosphorylates RVS167, linking cyclin-CDK activity with organization of the actin cytoskeleton.3 Publications

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • regulation of establishment or maintenance of cell polarity Source: SGD
  • septin ring organization Source: SGD

Keywordsi

Molecular functionCyclin
Biological processCell cycle, Cell division

Enzyme and pathway databases

BioCyciYEAST:G3O-29526-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PHO85 cyclin-2
Alternative name(s):
Cyclin HCS26 homolog
G1/S-specific cyclin PCL2
Gene namesi
Name:PCL2
Synonyms:CLN4
Ordered Locus Names:YDL127W
ORF Names:D2223
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL127W.
SGDiS000002285. PCL2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805001 – 308PHO85 cyclin-2Add BLAST308

Proteomic databases

PRIDEiP25693.

PTM databases

iPTMnetiP25693.

Expressioni

Inductioni

By transcription factors SBF (SWI4-SWI6 cell-cycle box binding factor) and SWI5 in a cell cycle-regulated manner. Peaks in G1 phase.2 Publications

Interactioni

Subunit structurei

Forms a cyclin-CDK complex with PHO85. Interacts with RVS167.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31936. 85 interactors.
DIPiDIP-1495N.
IntActiP25693. 8 interactors.
MINTiMINT-370014.
STRINGi4932.YDL127W.

Structurei

3D structure databases

ProteinModelPortaliP25693.
SMRiP25693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 146Cyclin N-terminalAdd BLAST129

Sequence similaritiesi

Belongs to the cyclin family. PCL1,2 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004809.
HOGENOMiHOG000000866.
InParanoidiP25693.
KOiK06657.
OMAiIETTRHR.
OrthoDBiEOG092C3KSL.

Family and domain databases

CDDicd00043. CYCLIN. 1 hit.
Gene3Di1.10.472.10. 1 hit.
InterProiView protein in InterPro
IPR013763. Cyclin-like.
IPR036915. Cyclin-like_sf.
IPR006671. Cyclin_N.
IPR012104. PHO85_cyclin_1/2/9.
PfamiView protein in Pfam
PF00134. Cyclin_N. 1 hit.
PIRSFiPIRSF016511. Cyclin_Pcl. 1 hit.
SMARTiView protein in SMART
SM00385. CYCLIN. 1 hit.
SUPFAMiSSF47954. SSF47954. 1 hit.

Sequencei

Sequence statusi: Complete.

P25693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNYEALLKF NRKAVSKEMV QYLASTTASI IKIKKTNSMI DIALPAPPLT
60 70 80 90 100
KFINRLIKHS NVQTPTLMAT SVYLAKLRSI IPSNVYGIET TRHRIFLGCL
110 120 130 140 150
ILAAKTLNDS SPLNKHWAEY TDGLLILREV NTIERELLEY FDWDVTISTD
160 170 180 190 200
DLITCLSPFL KPIKEEQLYK SQRDCRTLKN FSAQEKDIVN KTSISHSRSS
210 220 230 240 250
SNMSIPSLAS TSTLSTLESR RSNLSNYSNR IRTLPELHES NNISDKFSPR
260 270 280 290 300
TYNIDSKHDN KENRPIPTIK PFNFSKARPV ILKTGLNKQI IKEDTKVKKS

NWSNYFKS
Length:308
Mass (Da):35,207
Last modified:October 1, 1996 - v2
Checksum:i914BE1E2B92858CA
GO

Sequence cautioni

The sequence CAA98695 differs from that shown. Reason: Frameshift at position 263.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56956 Genomic DNA. Translation: CAA40277.1.
Z74175 Genomic DNA. Translation: CAA98695.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA11733.1.
PIRiS67670.
RefSeqiNP_010156.2. NM_001180186.1.

Genome annotation databases

EnsemblFungiiYDL127W; YDL127W; YDL127W.
GeneIDi851430.
KEGGisce:YDL127W.

Similar proteinsi

Entry informationi

Entry nameiPCL2_YEAST
AccessioniPrimary (citable) accession number: P25693
Secondary accession number(s): D6VRM3, Q07554
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names