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Reviewed, UniProtKB/Swiss-Prot P25688 (URIC_MOUSE)

Last modified June 16, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Uricase
    EC=1.7.3.3
Alternative name(s):
    Urate oxidase
Gene names
Name: Uox
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which spontaneously decomposes to form allantoin.

Catalytic activity

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.

Pathway

Purine metabolism; uric acid degradation; (S)-allantoin from uric acid: step 1/3.

Subcellular location

Peroxisome.

Post-translational modification

Acetylation of Lys-118, Lys-164 and Lys-290 is observed in liver mitochondria from fasted mice but not from fed mice.

Sequence similarities

Belongs to the uricase family.

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Ontologies

Keywords
   Biological processPurine metabolism
   Cellular componentPeroxisome
   Molecular functionOxidoreductase
   PTMAcetylation
Phosphoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

purine base metabolic process

Traceable author statement. Source: MGI

   Cellular componentmitochondrion

Inferred from direct assay. Source: MGI

peroxisome

Traceable author statement. Source: MGI

   Molecular functionurate oxidase activity

Traceable author statement. Source: MGI

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 303302Uricase
PRO_0000165986

Regions

Region234 – 2352Substrate binding By similarity
Motif301 – 3033Microbody targeting signal Potential

Sites

Active site1871Charge relay system By similarity
Active site2351Charge relay system By similarity
Binding site681Substrate By similarity
Binding site1871Substrate By similarity

Amino acid modifications

Modified residue21N-acetylalanine Ref.3
Modified residue361N6-acetyllysine Ref.4
Modified residue391Phosphoserine Ref.5
Modified residue551N6-acetyllysine Ref.4
Modified residue1181N6-acetyllysine Ref.4
Modified residue1221N6-acetyllysine Ref.4
Modified residue1641N6-acetyllysine Ref.4
Modified residue1851N6-acetyllysine Ref.4
Modified residue2201N6-acetyllysine Ref.4
Modified residue2271N6-acetyllysine Ref.4
Modified residue2311Phosphoserine Ref.5
Modified residue2771N6-acetyllysine Ref.4
Modified residue2901N6-acetyllysine Ref.4
Modified residue3011Phosphoserine Ref.5

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Sequences

Sequence LengthMass (Da)Tools
P25688-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: F521383D05370FA2

FASTA30335,039
        10         20         30         40         50         60 
MAHYHDNYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT LRSKKDYLHG 

        70         80         90        100        110        120 
DNSDIIPTDT IKNTVHVLAK LRGIRNIETF AMNICEHFLS SFNHVTRAHV YVEEVPWKRF 

       130        140        150        160        170        180 
EKNGIKHVHA FIHTPTGTHF CEVEQMRNGP PVIHSGIKDL KVLKTTQSGF EGFLKDQFTT 

       190        200        210        220        230        240 
LPEVKDRCFA TQVYCKWRYQ RRDVDFEAIW GAVRDIVLQK FAGPYDKGEY SPSVQKTLYD 

       250        260        270        280        290        300 
IQVLSLSQLP EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVKRKLP 


SRL

« Hide

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References

« Hide 'large scale' references
[1]"Urate oxidase: primary structure and evolutionary implications."
Wu X., Lee C.C., Muzny D.M., Caskey C.T.
Proc. Natl. Acad. Sci. U.S.A. 86:9412-9416(1989) [PubMed: 2594778] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[3]Kanor S., Bienvenut W.V.
Submitted (OCT-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-18; 42-52; 56-80; 86-107; 148-158; 165-185; 188-196; 203-214 AND 221-236, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Liver.
[4]"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey."
Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y.
Mol. Cell 23:607-618(2006) [PubMed: 16916647] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-36; LYS-55; LYS-118; LYS-122; LYS-164; LYS-185; LYS-220; LYS-227; LYS-277 AND LYS-290, MASS SPECTROMETRY.
Tissue: Liver.
[5]"Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS."
Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y.
Mol. Cell. Proteomics 6:669-676(2007) [PubMed: 17208939] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; SER-231 AND SER-301, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M27695 mRNA. Translation: AAA40538.1.
BC019771 mRNA. Translation: AAH19771.1.
IPIIPI00223367.
PIRB36227.
RefSeqNP_033500.1.
UniGeneMm.10865

3D structure databases

HSSPHSSP built from PDB template 1UOX based on UniProtKB Q00511.
ModBaseSearch...

PTM databases

PhosphoSiteP25688.

2-D gel databases

SWISS-2DPAGEP25688.

Genome annotation databases

EnsemblENSMUSG00000028186. Mus musculus. [Contig view]
GeneID22262.
KEGGmmu:22262.

Organism-specific databases

MGIMGI:98907. Uox.

Phylogenomic databases

HOGENOMP25688.
HOVERGENP25688.
OMAP25688. KTTQSGF.

Enzyme and pathway databases

BRENDA1.7.3.3. 244.

Gene expression databases

ArrayExpressP25688.
BgeeP25688.
CleanExMM_UOX.
GermOnlineENSMUSG00000028186. Mus musculus.

Family and domain databases

InterProIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
Gene3DG3DSA:3.10.270.10. Uricase. 1 hit.
PANTHERPTHR10395:SF1. Uricase. 1 hit.
PfamPF01014. Uricase. 2 hits.
[Graphical view]
PRINTSPR00093. URICASE.
ProDomPD003367. Uricase. 2 hits.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR03383. urate_oxi. 1 hit.
PROSITEPS00366. URICASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio302359.
SOURCESearch...

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Entry information

Entry nameURIC_MOUSE
AccessionPrimary (citable) accession number: P25688
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents