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Protein

Uricase

Gene

Uox

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.

Catalytic activityi

Urate + O2 + H2O = 5-hydroxyisourate + H2O2.

Pathwayi: urate degradation

This protein is involved in step 1 of the subpathway that synthesizes (S)-allantoin from urate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uricase (Uox), Uricase (Uox)
  2. 5-hydroxyisourate hydrolase (Urah)
  3. 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase (Urad)
This subpathway is part of the pathway urate degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-allantoin from urate, the pathway urate degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei187Charge relay systemBy similarity1
Binding sitei187SubstrateBy similarity1
Active sitei235Charge relay systemBy similarity1

GO - Molecular functioni

  • urate oxidase activity Source: MGI

GO - Biological processi

  • allantoin biosynthetic process Source: GO_Central
  • purine nucleobase metabolic process Source: MGI
  • urate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Names & Taxonomyi

Protein namesi
Recommended name:
Uricase (EC:1.7.3.3)
Alternative name(s):
Urate oxidase
Gene namesi
Name:Uox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:98907. Uox.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
  • peroxisome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001659862 – 303UricaseAdd BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei10N6-acetyllysine; alternateCombined sources1
Modified residuei10N6-succinyllysine; alternateCombined sources1
Modified residuei23N6-acetyllysine; alternateCombined sources1
Modified residuei23N6-succinyllysine; alternateCombined sources1
Modified residuei27N6-acetyllysineCombined sources1
Modified residuei36N6-acetyllysineCombined sources1
Modified residuei39PhosphoserineCombined sources1
Modified residuei63PhosphoserineCombined sources1
Modified residuei118N6-acetyllysineCombined sources1
Modified residuei122N6-acetyllysineCombined sources1
Modified residuei164N6-acetyllysineCombined sources1
Modified residuei175N6-acetyllysineCombined sources1
Modified residuei185N6-acetyllysineCombined sources1
Modified residuei220N6-acetyllysine; alternateCombined sources1
Modified residuei220N6-succinyllysine; alternateCombined sources1
Modified residuei227N6-acetyllysine; alternateCombined sources1
Modified residuei227N6-succinyllysine; alternateCombined sources1
Modified residuei231PhosphoserineCombined sources1
Modified residuei277N6-acetyllysineCombined sources1
Modified residuei288PhosphotyrosineCombined sources1

Post-translational modificationi

Acetylation of Lys-118, Lys-164 and Lys-290 is observed in liver mitochondria from fasted mice but not from fed mice. May be deacetylated by Sirt5; however it is unclear whether Sirt5 mediates deacetylation or desuccinylation of Uox; additional evidence is required to validate these results (PubMed:23085393).2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP25688.
PeptideAtlasiP25688.
PRIDEiP25688.

2D gel databases

SWISS-2DPAGEP25688.

PTM databases

iPTMnetiP25688.
PhosphoSitePlusiP25688.
SwissPalmiP25688.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028186.
CleanExiMM_UOX.
ExpressionAtlasiP25688. baseline and differential.
GenevisibleiP25688. MM.

Interactioni

Protein-protein interaction databases

IntActiP25688. 6 interactors.
MINTiMINT-4139643.
STRINGi10090.ENSMUSP00000029837.

Structurei

3D structure databases

ProteinModelPortaliP25688.
SMRiP25688.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 69Substrate bindingBy similarity2
Regioni234 – 235Substrate bindingBy similarity2

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi301 – 303Microbody targeting signalSequence analysis3

Sequence similaritiesi

Belongs to the uricase family.Curated

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
GeneTreeiENSGT00390000010863.
HOGENOMiHOG000250659.
HOVERGENiHBG018060.
InParanoidiP25688.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG091G0E99.
PhylomeDBiP25688.
TreeFamiTF323438.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25688-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHYHDNYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT
60 70 80 90 100
LRSKKDYLHG DNSDIIPTDT IKNTVHVLAK LRGIRNIETF AMNICEHFLS
110 120 130 140 150
SFNHVTRAHV YVEEVPWKRF EKNGIKHVHA FIHTPTGTHF CEVEQMRNGP
160 170 180 190 200
PVIHSGIKDL KVLKTTQSGF EGFLKDQFTT LPEVKDRCFA TQVYCKWRYQ
210 220 230 240 250
RRDVDFEAIW GAVRDIVLQK FAGPYDKGEY SPSVQKTLYD IQVLSLSQLP
260 270 280 290 300
EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVKRKLP

SRL
Length:303
Mass (Da):35,039
Last modified:January 23, 2007 - v2
Checksum:iF521383D05370FA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27695 mRNA. Translation: AAA40538.1.
BC019771 mRNA. Translation: AAH19771.1.
CCDSiCCDS17905.1.
PIRiB36227.
RefSeqiNP_033500.1. NM_009474.5.
UniGeneiMm.10865.

Genome annotation databases

EnsembliENSMUST00000029837; ENSMUSP00000029837; ENSMUSG00000028186.
GeneIDi22262.
KEGGimmu:22262.
UCSCiuc008rrp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27695 mRNA. Translation: AAA40538.1.
BC019771 mRNA. Translation: AAH19771.1.
CCDSiCCDS17905.1.
PIRiB36227.
RefSeqiNP_033500.1. NM_009474.5.
UniGeneiMm.10865.

3D structure databases

ProteinModelPortaliP25688.
SMRiP25688.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP25688. 6 interactors.
MINTiMINT-4139643.
STRINGi10090.ENSMUSP00000029837.

PTM databases

iPTMnetiP25688.
PhosphoSitePlusiP25688.
SwissPalmiP25688.

2D gel databases

SWISS-2DPAGEP25688.

Proteomic databases

PaxDbiP25688.
PeptideAtlasiP25688.
PRIDEiP25688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029837; ENSMUSP00000029837; ENSMUSG00000028186.
GeneIDi22262.
KEGGimmu:22262.
UCSCiuc008rrp.1. mouse.

Organism-specific databases

CTDi391051.
MGIiMGI:98907. Uox.

Phylogenomic databases

eggNOGiKOG1599. Eukaryota.
COG3648. LUCA.
GeneTreeiENSGT00390000010863.
HOGENOMiHOG000250659.
HOVERGENiHBG018060.
InParanoidiP25688.
KOiK00365.
OMAiKDRCFAT.
OrthoDBiEOG091G0E99.
PhylomeDBiP25688.
TreeFamiTF323438.

Enzyme and pathway databases

UniPathwayiUPA00394; UER00650.

Miscellaneous databases

PROiP25688.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028186.
CleanExiMM_UOX.
ExpressionAtlasiP25688. baseline and differential.
GenevisibleiP25688. MM.

Family and domain databases

InterProiIPR002042. Uricase.
IPR019842. Uricase_CS.
[Graphical view]
PfamiPF01014. Uricase. 2 hits.
[Graphical view]
PIRSFiPIRSF000241. Urate_oxidase. 1 hit.
PRINTSiPR00093. URICASE.
TIGRFAMsiTIGR03383. urate_oxi. 1 hit.
PROSITEiPS00366. URICASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURIC_MOUSE
AccessioniPrimary (citable) accession number: P25688
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.