ID   DNJB2_HUMAN             Reviewed;         324 AA.
AC   P25686; A8K9P6; Q8IUK1; Q8IUK2; Q96F52;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 3.
DT   25-JAN-2012, entry version 110.
DE   RecName: Full=DnaJ homolog subfamily B member 2;
DE   AltName: Full=DnaJ protein homolog 1;
DE   AltName: Full=Heat shock 40 kDa protein 3;
DE   AltName: Full=Heat shock protein J1;
DE            Short=HSJ-1;
GN   Name=DNAJB2; Synonyms=HSJ1, HSPF3;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
RC   TISSUE=Brain;
RX   MEDLINE=92287055; PubMed=1599432;
RA   Cheetham M.E., Brion J.-P., Anderton B.H.;
RT   "Human homologues of the bacterial heat-shock protein DnaJ are
RT   preferentially expressed in neurons.";
RL   Biochem. J. 284:469-476(1992).
RN   [2]
RP   SEQUENCE REVISION TO 214.
RA   Cheetham M.E.;
RL   Submitted (JUL-1998) to UniProtKB.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RA   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA   Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA   Phelan M., Farmer A.;
RT   "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT   vector.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Lung;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC   TISSUE=Colon, and Lymph;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
RX   PubMed=14759258; DOI=10.1186/gb-2004-5-2-r8;
RA   Hillman R.T., Green R.E., Brenner S.E.;
RT   "An unappreciated role for RNA surveillance.";
RL   Genome Biol. 5:R8.1-R8.16(2004).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC         Comment=Experimental confirmation may be lacking for some
CC         isoforms;
CC       Name=3;
CC         IsoId=P25686-3; Sequence=Displayed;
CC         Note=May be produced at very low levels due to a premature stop
CC         codon in the mRNA, leading to nonsense-mediated mRNA decay.
CC         Ref.1 (AAA09035) sequence differs from that shown due to a
CC         frameshift in position 288;
CC       Name=2; Synonyms=HSJ1A;
CC         IsoId=P25686-2; Sequence=VSP_001286, VSP_001287;
CC   -!- TISSUE SPECIFICITY: Brain (neuronal layers). Weakly, in skeletal
CC       muscle and spleen.
CC   -!- SIMILARITY: Contains 1 J domain.
CC   -!- SIMILARITY: Contains 2 UIM (ubiquitin-interacting motif) repeats.
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DR   EMBL; S37375; AAA09034.1; -; mRNA.
DR   EMBL; S37374; AAA09035.1; ALT_FRAME; mRNA.
DR   EMBL; X63368; CAA44968.2; -; Genomic_DNA.
DR   EMBL; X63368; CAA44969.2; -; Genomic_DNA.
DR   EMBL; BT007088; AAP35751.1; -; mRNA.
DR   EMBL; AK292761; BAF85450.1; -; mRNA.
DR   EMBL; BC011609; AAH11609.1; -; mRNA.
DR   EMBL; BC047056; AAH47056.1; -; mRNA.
DR   IPI; IPI00220102; -.
DR   IPI; IPI00874105; -.
DR   PIR; S23508; S23508.
DR   RefSeq; NP_001034639.1; NM_001039550.1.
DR   RefSeq; NP_006727.2; NM_006736.5.
DR   UniGene; Hs.77768; -.
DR   ProteinModelPortal; P25686; -.
DR   SMR; P25686; 1-69.
DR   DIP; DIP-29051N; -.
DR   MINT; MINT-1192251; -.
DR   STRING; P25686; -.
DR   PhosphoSite; P25686; -.
DR   DMDM; 158518384; -.
DR   PRIDE; P25686; -.
DR   Ensembl; ENST00000336576; ENSP00000338019; ENSG00000135924.
DR   GeneID; 3300; -.
DR   KEGG; hsa:3300; -.
DR   UCSC; uc002vkw.1; human.
DR   UCSC; uc002vkx.1; human.
DR   CTD; 3300; -.
DR   GeneCards; GC02P220143; -.
DR   HGNC; HGNC:5228; DNAJB2.
DR   HPA; HPA036268; -.
DR   MIM; 604139; gene.
DR   neXtProt; NX_P25686; -.
DR   PharmGKB; PA27415; -.
DR   eggNOG; prNOG13146; -.
DR   HOGENOM; HBG716471; -.
DR   HOVERGEN; HBG066998; -.
DR   InParanoid; P25686; -.
DR   OMA; SINGVPD; -.
DR   OrthoDB; EOG4QJRPN; -.
DR   PhylomeDB; P25686; -.
DR   NextBio; 13093; -.
DR   ArrayExpress; P25686; -.
DR   Bgee; P25686; -.
DR   CleanEx; HS_DNAJB2; -.
DR   Genevestigator; P25686; -.
DR   GermOnline; ENSG00000135924; Homo sapiens.
DR   GO; GO:0016234; C:inclusion body; IDA:BHF-UCL.
DR   GO; GO:0031072; F:heat shock protein binding; IEA:InterPro.
DR   GO; GO:0030544; F:Hsp70 protein binding; IPI:BHF-UCL.
DR   GO; GO:0031593; F:polyubiquitin binding; IDA:BHF-UCL.
DR   GO; GO:0070628; F:proteasome binding; IDA:BHF-UCL.
DR   GO; GO:0005515; F:protein binding; IPI:BHF-UCL.
DR   GO; GO:0051082; F:unfolded protein binding; TAS:ProtInc.
DR   GO; GO:0030433; P:ER-associated protein catabolic process; IDA:BHF-UCL.
DR   GO; GO:0030308; P:negative regulation of cell growth; IGI:UniProtKB.
DR   GO; GO:0008285; P:negative regulation of cell proliferation; IGI:UniProtKB.
DR   GO; GO:0090084; P:negative regulation of inclusion body assembly; IDA:BHF-UCL.
DR   GO; GO:0090086; P:negative regulation of protein deubiquitination; IDA:BHF-UCL.
DR   GO; GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IDA:BHF-UCL.
DR   GO; GO:0031398; P:positive regulation of protein ubiquitination; IDA:BHF-UCL.
DR   GO; GO:0006457; P:protein folding; TAS:ProtInc.
DR   GO; GO:0006986; P:response to unfolded protein; TAS:ProtInc.
DR   InterPro; IPR001623; DnaJ_N.
DR   InterPro; IPR018253; Heat_shock_DnaJ_CS.
DR   InterPro; IPR003095; Hsp_DnaJ.
DR   InterPro; IPR003903; Ubiquitin-int_motif.
DR   Gene3D; G3DSA:1.10.287.110; DnaJ_N; 1.
DR   KO; K09508; -.
DR   Pfam; PF00226; DnaJ; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SMART; SM00726; UIM; 2.
DR   SUPFAM; SSF46565; DnaJ_N; 1.
DR   PROSITE; PS00636; DNAJ_1; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
DR   PROSITE; PS50330; UIM; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Chaperone; Complete proteome; Polymorphism;
KW   Reference proteome; Repeat.
FT   CHAIN         1    324       DnaJ homolog subfamily B member 2.
FT                                /FTId=PRO_0000071018.
FT   DOMAIN        1     71       J.
FT   REPEAT      210    226       UIM 1.
FT   REPEAT      250    269       UIM 2.
FT   VAR_SEQ     275    277       GGR -> DVF (in isoform 2).
FT                                /FTId=VSP_001286.
FT   VAR_SEQ     278    324       Missing (in isoform 2).
FT                                /FTId=VSP_001287.
FT   VARIANT     270    270       G -> R (in dbSNP:rs34127289).
FT                                /FTId=VAR_048910.
FT   CONFLICT    214    214       L -> R (in Ref. 1; AAA09034/AAA09035).
SQ   SEQUENCE   324 AA;  35580 MW;  0154ED3E29F34B4A CRC64;
     MASYYEILDV PRSASADDIK KAYRRKALQW HPDKNPDNKE FAEKKFKEVA EAYEVLSDKH
     KREIYDRYGR EGLTGTGTGP SRAEAGSGGP GFTFTFRSPE EVFREFFGSG DPFAELFDDL
     GPFSELQNRG SRHSGPFFTF SSSFPGHSDF SSSSFSFSPG AGAFRSVSTS TTFVQGRRIT
     TRRIMENGQE RVEVEEDGQL KSVTINGVPD DLALGLELSR REQQPSVTSR SGGTQVQQTP
     ASCPLDSDLS EDEDLQLAMA YSLSEMEAAG KKPAGGREAQ HRRQGRPKAQ HQDPGLGGTQ
     EGARGEATKR SPSPEEKASR CLIL
//