P25686 (DNJB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 110.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: DnaJ homolog subfamily B member 2 Alternative name(s): DnaJ protein homolog 1 Heat shock 40 kDa protein 3 Heat shock protein J1 Short name=HSJ-1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 324 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Tissue specificity | Brain (neuronal layers). Weakly, in skeletal muscle and spleen. |
| Sequence similarities | Contains 1 J domain. Contains 2 UIM (ubiquitin-interacting motif) repeats. |
| Sequence caution | Isoform 3: The sequence AAA09035.1 differs from that shown. Reason: Frameshift at position 288. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] Note: Experimental confirmation may be lacking for some isoforms. | ||||||
| Isoform 3 (identifier: P25686-3) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. | ||||||
| Isoform 2 (identifier: P25686-2) Also known as: HSJ1A; The sequence of this isoform differs from the canonical sequence as follows: 275-277: GGR → DVF 278-324: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 324 | 324 | DnaJ homolog subfamily B member 2 | PRO_0000071018 | |||||
Regions | |||||||||
| Domain | 1 – 71 | 71 | J | ||||||
| Repeat | 210 – 226 | 17 | UIM 1 | ||||||
| Repeat | 250 – 269 | 20 | UIM 2 | ||||||
Natural variations | |||||||||
| Alternative sequence | 275 – 277 | 3 | GGR → DVF in isoform 2. | VSP_001286 | |||||
| Alternative sequence | 278 – 324 | 47 | Missing in isoform 2. | VSP_001287 | |||||
| Natural variant | 270 | 1 | G → R. Corresponds to variant rs34127289 [ dbSNP | Ensembl ]. | VAR_048910 | |||||
Experimental info | |||||||||
| Sequence conflict | 214 | 1 | L → R in AAA09034. Ref.1 | ||||||
| Sequence conflict | 214 | 1 | L → R in AAA09035. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Human homologues of the bacterial heat-shock protein DnaJ are preferentially expressed in neurons." Cheetham M.E., Brion J.-P., Anderton B.H. Biochem. J. 284:469-476(1992) [PubMed: 1599432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2). Tissue: Brain. |
| [2] | Cheetham M.E. Submitted (JUL-1998) to UniProtKB Cited for: SEQUENCE REVISION TO 214. |
| [3] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). |
| [4] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Colon and Lymph. |
| [6] | "An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:R8.1-R8.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S). |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | S37375 mRNA. Translation: AAA09034.1. S37374 mRNA. Translation: AAA09035.1. Frameshift. X63368 Genomic DNA. Translation: CAA44968.2. X63368 Genomic DNA. Translation: CAA44969.2. BT007088 mRNA. Translation: AAP35751.1. AK292761 mRNA. Translation: BAF85450.1. BC011609 mRNA. Translation: AAH11609.1. BC047056 mRNA. Translation: AAH47056.1. |
| IPI | IPI00220102. IPI00874105. |
| PIR | S23508. |
| RefSeq | NP_001034639.1. NM_001039550.1. NP_006727.2. NM_006736.5. |
| UniGene | Hs.77768. |
3D structure databases | |
| ProteinModelPortal | P25686. |
| SMR | P25686. Positions 1-69. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-29051N. |
| MINT | MINT-1192251. |
| STRING | P25686. |
PTM databases | |
| PhosphoSite | P25686. |
Polymorphism databases | |
| DMDM | 158518384. |
Proteomic databases | |
| PRIDE | P25686. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000336576; ENSP00000338019; ENSG00000135924. |
| GeneID | 3300. |
| KEGG | hsa:3300. |
| UCSC | uc002vkw.1. human. uc002vkx.1. human. |
Organism-specific databases | |
| CTD | 3300. |
| GeneCards | GC02P220143. |
| HGNC | HGNC:5228. DNAJB2. |
| HPA | HPA036268. |
| MIM | 604139. gene. |
| neXtProt | NX_P25686. |
| PharmGKB | PA27415. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG13146. |
| HOGENOM | HBG716471. |
| HOVERGEN | HBG066998. |
| InParanoid | P25686. |
| OMA | SINGVPD. |
| OrthoDB | EOG4QJRPN. |
| PhylomeDB | P25686. |
Gene expression databases | |
| ArrayExpress | P25686. |
| Bgee | P25686. |
| CleanEx | HS_DNAJB2. |
| Genevestigator | P25686. |
| GermOnline | ENSG00000135924. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001623. DnaJ_N. IPR018253. Heat_shock_DnaJ_CS. IPR003095. Hsp_DnaJ. IPR003903. Ubiquitin-int_motif. [Graphical view] |
| Gene3D | G3DSA:1.10.287.110. DnaJ_N. 1 hit. |
| KO | K09508. |
| Pfam | PF00226. DnaJ. 1 hit. [Graphical view] |
| PRINTS | PR00625. JDOMAIN. |
| SMART | SM00271. DnaJ. 1 hit. SM00726. UIM. 2 hits. [Graphical view] |
| SUPFAM | SSF46565. DnaJ_N. 1 hit. |
| PROSITE | PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. PS50330. UIM. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 13093. |
| SOURCE | Search... |
Entry information
| Entry name | DNJB2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P25686 Secondary accession number(s): A8K9P6 Q96F52 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 2 Human chromosome 2: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with