P25686 (DNJB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ homolog subfamily B member 2

Alternative name(s):
DnaJ protein homolog 1
Heat shock 40 kDa protein 3
Heat shock protein J1
Short name=HSJ-1

Gene names

Name:	DNAJB2
Synonyms:	HSJ1, HSPF3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Tissue specificity	Brain (neuronal layers). Weakly, in skeletal muscle and spleen.
Sequence similarities	Contains 1 J domain. Contains 2 UIM (ubiquitin-interacting motif) repeats.
Sequence caution	Isoform 3: The sequence AAA09035.1 differs from that shown. Reason: Frameshift at position 288.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER-associated protein catabolic process Inferred from direct assay. Source: BHF-UCL negative regulation of cell growth Inferred from genetic interaction. Source: UniProtKB negative regulation of cell proliferation Inferred from genetic interaction. Source: UniProtKB negative regulation of inclusion body assembly Inferred from direct assay. Source: BHF-UCL negative regulation of protein deubiquitination Inferred from direct assay. Source: BHF-UCL positive regulation of proteasomal ubiquitin-dependent protein catabolic process Inferred from direct assay. Source: BHF-UCL positive regulation of protein ubiquitination Inferred from direct assay. Source: BHF-UCL protein folding Traceable author statement. Source: ProtInc response to unfolded protein Traceable author statement. Source: ProtInc
Cellular component	inclusion body Inferred from direct assay. Source: BHF-UCL
Molecular function	Hsp70 protein binding Inferred from physical interaction. Source: BHF-UCL heat shock protein binding Inferred from electronic annotation. Source: InterPro polyubiquitin binding Inferred from direct assay. Source: BHF-UCL proteasome binding Inferred from direct assay. Source: BHF-UCL protein binding Inferred from physical interaction. Source: BHF-UCL unfolded protein binding Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 3 (identifier: P25686-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 2 (identifier: P25686-2)

Also known as: HSJ1A;

The sequence of this isoform differs from the canonical sequence as follows:
     275-277: GGR → DVF
     278-324: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	DnaJ homolog subfamily B member 2		PRO_0000071018
Regions
	Domain	1 – 71	71	J
	Repeat	210 – 226	17	UIM 1
	Repeat	250 – 269	20	UIM 2
Natural variations
	Alternative sequence	275 – 277	3	GGR → DVF in isoform 2.		VSP_001286
	Alternative sequence	278 – 324	47	Missing in isoform 2.		VSP_001287
	Natural variant	270	1	G → R. Corresponds to variant rs34127289 [ dbSNP | Ensembl ].		VAR_048910
Experimental info
	Sequence conflict	214	1	L → R in AAA09034. Ref.1
	Sequence conflict	214	1	L → R in AAA09035. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 [UniParc]. Last modified September 11, 2007. Version 3. Checksum: 0154ED3E29F34B4A	FASTA	324	35,580
	10 20 30 40 50 60 MASYYEILDV PRSASADDIK KAYRRKALQW HPDKNPDNKE FAEKKFKEVA EAYEVLSDKH 70 80 90 100 110 120 KREIYDRYGR EGLTGTGTGP SRAEAGSGGP GFTFTFRSPE EVFREFFGSG DPFAELFDDL 130 140 150 160 170 180 GPFSELQNRG SRHSGPFFTF SSSFPGHSDF SSSSFSFSPG AGAFRSVSTS TTFVQGRRIT 190 200 210 220 230 240 TRRIMENGQE RVEVEEDGQL KSVTINGVPD DLALGLELSR REQQPSVTSR SGGTQVQQTP 250 260 270 280 290 300 ASCPLDSDLS EDEDLQLAMA YSLSEMEAAG KKPAGGREAQ HRRQGRPKAQ HQDPGLGGTQ 310 320 EGARGEATKR SPSPEEKASR CLIL
	Isoform 2 (HSJ1A) [UniParc]. Checksum: 1C843287D7856CFB	FASTA	277	30,569
	10 20 30 40 50 60 MASYYEILDV PRSASADDIK KAYRRKALQW HPDKNPDNKE FAEKKFKEVA EAYEVLSDKH 70 80 90 100 110 120 KREIYDRYGR EGLTGTGTGP SRAEAGSGGP GFTFTFRSPE EVFREFFGSG DPFAELFDDL 130 140 150 160 170 180 GPFSELQNRG SRHSGPFFTF SSSFPGHSDF SSSSFSFSPG AGAFRSVSTS TTFVQGRRIT 190 200 210 220 230 240 TRRIMENGQE RVEVEEDGQL KSVTINGVPD DLALGLELSR REQQPSVTSR SGGTQVQQTP 250 260 270 ASCPLDSDLS EDEDLQLAMA YSLSEMEAAG KKPADVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human homologues of the bacterial heat-shock protein DnaJ are preferentially expressed in neurons." Cheetham M.E., Brion J.-P., Anderton B.H. Biochem. J. 284:469-476(1992) [PubMed: 1599432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2). Tissue: Brain.
[2]	Cheetham M.E. Submitted (JUL-1998) to UniProtKB Cited for: SEQUENCE REVISION TO 214.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Colon and Lymph.
[6]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:R8.1-R8.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S37375 mRNA. Translation: AAA09034.1. S37374 mRNA. Translation: AAA09035.1. Frameshift. X63368 Genomic DNA. Translation: CAA44968.2. X63368 Genomic DNA. Translation: CAA44969.2. BT007088 mRNA. Translation: AAP35751.1. AK292761 mRNA. Translation: BAF85450.1. BC011609 mRNA. Translation: AAH11609.1. BC047056 mRNA. Translation: AAH47056.1.
IPI	IPI00220102. IPI00874105.
PIR	S23508.
RefSeq	NP_001034639.1. NM_001039550.1. NP_006727.2. NM_006736.5.
UniGene	Hs.77768.
3D structure databases
ProteinModelPortal	P25686.
SMR	P25686. Positions 1-69.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29051N.
MINT	MINT-1192251.
STRING	P25686.
PTM databases
PhosphoSite	P25686.
Polymorphism databases
DMDM	158518384.
Proteomic databases
PRIDE	P25686.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336576; ENSP00000338019; ENSG00000135924.
GeneID	3300.
KEGG	hsa:3300.
UCSC	uc002vkw.1. human. uc002vkx.1. human.
Organism-specific databases
CTD	3300.
GeneCards	GC02P220143.
HGNC	HGNC:5228. DNAJB2.
HPA	HPA036268.
MIM	604139. gene.
neXtProt	NX_P25686.
PharmGKB	PA27415.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13146.
HOGENOM	HBG716471.
HOVERGEN	HBG066998.
InParanoid	P25686.
OMA	SINGVPD.
OrthoDB	EOG4QJRPN.
PhylomeDB	P25686.
Gene expression databases
ArrayExpress	P25686.
Bgee	P25686.
CleanEx	HS_DNAJB2.
Genevestigator	P25686.
GermOnline	ENSG00000135924. Homo sapiens.
Family and domain databases
InterPro	IPR001623. DnaJ_N. IPR018253. Heat_shock_DnaJ_CS. IPR003095. Hsp_DnaJ. IPR003903. Ubiquitin-int_motif. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit.
KO	K09508.
Pfam	PF00226. DnaJ. 1 hit. [Graphical view]
PRINTS	PR00625. JDOMAIN.
SMART	SM00271. DnaJ. 1 hit. SM00726. UIM. 2 hits. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit.
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. PS50330. UIM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	13093.
SOURCE	Search...

Entry information

Entry name

DNJB2_HUMAN

Accession

Primary (citable) accession number: P25686
Secondary accession number(s): A8K9P6 , Q8IUK1, Q8IUK2, Q96F52

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	September 11, 2007
Last modified:	January 25, 2012
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.