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Reviewed, UniProtKB/Swiss-Prot P25686 (DNJB2_HUMAN)

Last modified November 3, 2009. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    DnaJ homolog subfamily B member 2
Alternative name(s):
    Heat shock 40 kDa protein 3
    DnaJ protein homolog 1
    HSJ-1
Gene names
Name: DNAJB2
Synonyms: HSJ1, HSPF3
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Tissue specificity

Brain (neuronal layers). Weakly, in skeletal muscle and spleen.

Sequence similarities

Contains 1 J domain.

Contains 2 UIM (ubiquitin-interacting motif) repeats.

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Ontologies

Keywords
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
   Molecular functionChaperone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processprotein folding Ref.1

Traceable author statement. Source: ProtInc

response to unfolded protein Ref.1

Traceable author statement. Source: ProtInc

   Molecular functionheat shock protein binding

Inferred from electronic annotation. Source: InterPro

unfolded protein binding Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 3 (identifier: P25686-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. Ref.1 (AAA09035) sequence differs from that shown due to a frameshift at position 288.
Isoform 2 (identifier: P25686-2)

Also known as: HSJ1A;

The sequence of this isoform differs from the canonical sequence as follows:
     275-277: GGR → DVF
     278-324: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324DnaJ homolog subfamily B member 2
PRO_0000071018

Regions

Domain1 – 7171J
Repeat210 – 22617UIM 1
Repeat250 – 26920UIM 2

Natural variations

Alternative sequence275 – 2773GGR → DVF in isoform 2.
VSP_001286
Alternative sequence278 – 32447Missing in isoform 2.
VSP_001287
Natural variant2701G → R: dbSNP rs34127289.
VAR_048910

Experimental info

Sequence conflict2141L → R in AAA09034. Ref.1
Sequence conflict2141L → R in AAA09035. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Isoform 3 [UniParc].

Last modified September 11, 2007. Version 3.
Checksum: 0154ED3E29F34B4A

FASTA32435,580
        10         20         30         40         50         60 
MASYYEILDV PRSASADDIK KAYRRKALQW HPDKNPDNKE FAEKKFKEVA EAYEVLSDKH 

        70         80         90        100        110        120 
KREIYDRYGR EGLTGTGTGP SRAEAGSGGP GFTFTFRSPE EVFREFFGSG DPFAELFDDL 

       130        140        150        160        170        180 
GPFSELQNRG SRHSGPFFTF SSSFPGHSDF SSSSFSFSPG AGAFRSVSTS TTFVQGRRIT 

       190        200        210        220        230        240 
TRRIMENGQE RVEVEEDGQL KSVTINGVPD DLALGLELSR REQQPSVTSR SGGTQVQQTP 

       250        260        270        280        290        300 
ASCPLDSDLS EDEDLQLAMA YSLSEMEAAG KKPAGGREAQ HRRQGRPKAQ HQDPGLGGTQ 

       310        320 
EGARGEATKR SPSPEEKASR CLIL

« Hide

Isoform 2 (HSJ1A).

Checksum: 1C843287D7856CFB
Show »

FASTA27730,569

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References

« Hide 'large scale' references
[1]"Human homologues of the bacterial heat-shock protein DnaJ are preferentially expressed in neurons."
Cheetham M.E., Brion J.-P., Anderton B.H.
Biochem. J. 284:469-476(1992) [PubMed: 1599432] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
Tissue: Brain.
[2]Cheetham M.E.
Submitted (JUL-1998) to UniProtKB
Cited for: SEQUENCE REVISION TO 214.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Lung.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Colon and Lymph.
[6]"An unappreciated role for RNA surveillance."
Hillman R.T., Green R.E., Brenner S.E.
Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004) [PubMed: 14759258] [Abstract]
Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
+Additional computationally mapped references.

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Cross-references

Sequence databases

S37375 mRNA. Translation: AAA09034.1.
S37374 mRNA. Translation: AAA09035.1. Frameshift.
X63368 Genomic DNA. Translation: CAA44968.2.
X63368 Genomic DNA. Translation: CAA44969.2.
BT007088 mRNA. Translation: AAP35751.1.
AK292761 mRNA. Translation: BAF85450.1.
BC011609 mRNA. Translation: AAH11609.1.
BC047056 mRNA. Translation: AAH47056.1.
IPIIPI00220102.
IPI00874105.
PIRS23508.
RefSeqNP_001034639.1.
NP_006727.2.
UniGeneHs.77768

3D structure databases

HSSPHSSP built from PDB template 1HDJ based on UniProtKB P25685.
SMRP25686. Positions 1-69.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:29051N.
STRINGP25686.

PTM databases

PhosphoSiteP25686.

Proteomic databases

PRIDEP25686.

Genome annotation databases

EnsemblENST00000336576; ENSP00000338019; ENSG00000135924; Homo sapiens. [Genome view]
ENST00000392086; ENSP00000375936; ENSG00000135924; Homo sapiens. [Genome view]
ENST00000392087; ENSP00000375937; ENSG00000135924; Homo sapiens. [Genome view]
ENST00000421532; ENSP00000395173; ENSG00000135924; Homo sapiens. [Genome view]
ENST00000425450; ENSP00000414796; ENSG00000135924; Homo sapiens. [Genome view]
ENST00000439026; ENSP00000387951; ENSG00000135924; Homo sapiens. [Genome view]
ENST00000442681; ENSP00000392790; ENSG00000135924; Homo sapiens. [Genome view]
GeneID3300.
KEGGhsa:3300.
UCSCuc002vkw.1. human.
uc002vkx.1. human.

Organism-specific databases

CTD3300.
GeneCardsGC02P219852.
H-InvDBHIX0002859.
HGNCHGNC:5228. DNAJB2.
MIM604139. gene.
PharmGKBPA27415.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP25686.
HOVERGENP25686.
OMAQQSVTSR.

Gene expression databases

ArrayExpressP25686.
BgeeP25686.
CleanExHS_DNAJB2.
GenevestigatorP25686.
GermOnlineENSG00000135924. Homo sapiens.

Family and domain databases

InterProIPR001623. DnaJ_N.
IPR018253. Heat_shock_DnaJ_CS.
IPR015609. Hsp40/DnaJ_Rel.
IPR003903. Ubiquitin-int_motif.
[Graphical view]
PANTHERPTHR11821. Hsp40/DnaJ_Rel. 1 hit.
PfamPF00226. DnaJ. 1 hit.
PF02809. UIM. 1 hit.
[Graphical view]
SMARTSM00271. DnaJ. 1 hit.
SM00726. UIM. 2 hits.
[Graphical view]
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS50330. UIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio13093.
SOURCESearch...

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Entry information

Entry nameDNJB2_HUMAN
AccessionPrimary (citable) accession number: P25686
Secondary accession number(s): A8K9P6 expand/collapse secondary AC list , Q8IUK1, Q8IUK2, Q96F52
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: September 11, 2007
Last modified: November 3, 2009
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents