Reviewed,
UniProtKB/Swiss-Prot P25685 (DNJB1_HUMAN)
Last modified
January 19, 2010.
Version 109.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: DnaJ homolog subfamily B member 1 Alternative name(s): Heat shock 40 kDa protein 1 Short name=Heat shock protein 40 Short name=HSP40 DnaJ protein homolog 1 HDJ-1 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 340 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. |
| Subunit structure | Interacts with DNAJC3. Ref.6 |
| Subcellular location | Cytoplasm. Nucleus. Nucleus › nucleolus. Note: Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. Ref.5 |
| Induction | By heat shock. Ref.5 |
| Sequence similarities | Contains 1 J domain. |
| Sequence caution | The sequence CAA44287.1 differs from that shown. Reason: Frameshift at positions 11, 28, 81 and 136. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Stress response |
| Cellular component | Cytoplasm Nucleus |
| Molecular function | Chaperone |
| PTM | Acetylation |
| Technical term | 3D-structure Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | response to unfolded protein Ref.3 Traceable author statement. Source: ProtInc |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolusInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | heat shock protein binding Inferred from electronic annotation. Source: InterPro unfolded protein bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||
Molecule processing | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.5 Ref.2 | ||||||||||||||||||
| Chain | 2 – 340 | 339 | DnaJ homolog subfamily B member 1 | PRO_0000071016 | |||||||||||||||||
Regions | |||||||||||||||||||||
| Domain | 2 – 70 | 69 | J | ||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||
| Modified residue | 44 | 1 | N6-acetyllysine Ref.8 | ||||||||||||||||||
Experimental info | |||||||||||||||||||||
| Sequence conflict | 68 | 1 | G → L in CAA44287. Ref.1 | ||||||||||||||||||
| Sequence conflict | 150 | 1 | R → C in CAA44287. Ref.1 | ||||||||||||||||||
| Sequence conflict | 183 | 1 | M → T in CAA44287. Ref.1 | ||||||||||||||||||
| Sequence conflict | 320 | 1 | V → A in CAA44287. Ref.1 | ||||||||||||||||||
Secondary structure | |||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||
| Helix | 5 – 8 | 4 | |||||||||||||||||||
| Helix | 16 – 28 | 13 | |||||||||||||||||||
| Turn | 32 – 34 | 3 | |||||||||||||||||||
| Helix | 40 – 53 | 14 | |||||||||||||||||||
| Helix | 57 – 65 | 9 | |||||||||||||||||||
| Helix | 68 – 70 | 3 | |||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A human homologue of the Escherichia coli DnaJ heat-shock protein." Raabe T., Manley J.L. Nucleic Acids Res. 19:6645-6645(1991) [PubMed: 1754405] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta. |
| [2] | "Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ." Ohtsuka K. Biochem. Biophys. Res. Commun. 197:235-240(1993) [PubMed: 8250930] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-48. Tissue: Placenta. |
| [3] | "Genomic cloning of a human heat shock protein 40 (Hsp40) gene (HSPF1) and its chromosomal localization to 19p13.2." Hata M., Okumura K., Seto M., Ohtsuka K. Genomics 38:446-449(1996) [PubMed: 8975727] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Lung. |
| [5] | "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells." Hattori H., Liu Y.-C., Tohnai I., Ueda M., Kaneda T., Kobayashi T., Tanabe K., Ohtsuka K. Cell Struct. Funct. 17:77-86(1992) [PubMed: 1586970] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-49, INDUCTION, SUBCELLULAR LOCATION. |
| [6] | "The cellular inhibitor of the PKR protein kinase, P58(IPK), is an influenza virus-activated co-chaperone that modulates heat shock protein 70 activity." Melville M.W., Tan S.-L., Wambach M., Song J., Morimoto R.I., Katze M.G. J. Biol. Chem. 274:3797-3803(1999) [PubMed: 9920933] [Abstract] Cited for: INTERACTION WITH DNAJC3. |
| [7] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [8] | "Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-44, MASS SPECTROMETRY. |
| [9] | "Nuclear magnetic resonance solution structure of the human Hsp40 (HDJ-1) J-domain." Qian Y.Q., Patel D., Hartl F.-U., McColl D.J. J. Mol. Biol. 260:224-235(1996) [PubMed: 8764402] [Abstract] Cited for: STRUCTURE BY NMR OF 1-76. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | X62421 mRNA. Translation: CAA44287.1. Frameshift. D49547 mRNA. Translation: BAA08495.1. D85429 Genomic DNA. Translation: BAA12819.1. BC002352 mRNA. Translation: AAH02352.1. BC019827 mRNA. Translation: AAH19827.1. | ||||||||||||||||||
| IPI | IPI00015947. | ||||||||||||||||||
| PIR | JN0912. S20062. | ||||||||||||||||||
| RefSeq | NP_006136.1. | ||||||||||||||||||
| UniGene | Hs.515210 | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| DIP | DIP-41180N. | ||||||||||||||||||
| IntAct | P25685. 6 interactions. | ||||||||||||||||||
| STRING | P25685. | ||||||||||||||||||
PTM databases | |||||||||||||||||||
| PhosphoSite | P25685. | ||||||||||||||||||
2-D gel databases | |||||||||||||||||||
| REPRODUCTION-2DPAGE | IPI00015947. | ||||||||||||||||||
Proteomic databases | |||||||||||||||||||
| PeptideAtlas | P25685. | ||||||||||||||||||
| PRIDE | P25685. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| Ensembl | ENST00000254322; ENSP00000254322; ENSG00000132002; Homo sapiens. [Genome view] | ||||||||||||||||||
| GeneID | 3337. | ||||||||||||||||||
| KEGG | hsa:3337. | ||||||||||||||||||
| UCSC | uc002myz.1. human. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| CTD | 3337. | ||||||||||||||||||
| GeneCards | GC19M014486. | ||||||||||||||||||
| H-InvDB | HIX0014838. | ||||||||||||||||||
| HGNC | HGNC:5270. DNAJB1. | ||||||||||||||||||
| HPA | CAB017450. | ||||||||||||||||||
| MIM | 604572. gene. | ||||||||||||||||||
| PharmGKB | PA27412. | ||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| eggNOG | prNOG06818. | ||||||||||||||||||
| HOGENOM | HBG635315. | ||||||||||||||||||
| HOVERGEN | P25685. | ||||||||||||||||||
| InParanoid | P25685. | ||||||||||||||||||
| OMA | GGFTNMN. | ||||||||||||||||||
| OrthoDB | EOG9NP9PN. | ||||||||||||||||||
| PhylomeDB | P25685. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| ArrayExpress | P25685. | ||||||||||||||||||
| Bgee | P25685. | ||||||||||||||||||
| CleanEx | HS_DNAJB1. | ||||||||||||||||||
| Genevestigator | P25685. | ||||||||||||||||||
| GermOnline | ENSG00000132002. Homo sapiens. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR002939. DnaJ_C. IPR001623. DnaJ_N. IPR018253. Heat_shock_DnaJ_CS. IPR008971. HSP40/DnaJ_pept-bd. IPR015609. Hsp40/DnaJ_Rel. IPR003095. Hsp_DnaJ. [Graphical view] | ||||||||||||||||||
| Gene3D | G3DSA:1.10.287.110. DnaJ_N. 1 hit. | ||||||||||||||||||
| PANTHER | PTHR11821. Hsp40/DnaJ_Rel. 1 hit. | ||||||||||||||||||
| Pfam | PF00226. DnaJ. 1 hit. PF01556. DnaJ_C. 1 hit. [Graphical view] | ||||||||||||||||||
| PRINTS | PR00625. DNAJPROTEIN. | ||||||||||||||||||
| SMART | SM00271. DnaJ. 1 hit. [Graphical view] | ||||||||||||||||||
| PROSITE | PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Other Resources | |||||||||||||||||||
| NextBio | 13208. | ||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||
Entry information
| Entry name | DNJB1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P25685 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 19 Human chromosome 19: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


