P25685 (DNJB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DnaJ homolog subfamily B member 1

Alternative name(s):
DnaJ protein homolog 1
Heat shock 40 kDa protein 1
Short name=HSP40
Short name=Heat shock protein 40
Human DnaJ protein 1
Short name=hDj-1

Gene names

Name:	DNAJB1
Synonyms:	DNAJ1, HDJ1, HSPF1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
Subunit structure	Interacts with DNAJC3. Interacts with SRPK1. Ref.6 Ref.7
Subcellular location	Cytoplasm. Nucleus. Nucleus › nucleolus. Note: Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. Ref.5
Induction	By heat shock. Ref.5
Sequence similarities	Contains 1 J domain.
Sequence caution	The sequence CAA44287.1 differs from that shown. Reason: Frameshift at positions 11, 28, 81 and 136.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm Nucleus
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	chaperone cofactor-dependent protein refolding Inferred from direct assay PubMed 18620420. Source: UniProtKB chaperone mediated protein folding requiring cofactor Inferred from electronic annotation. Source: Compara response to unfolded protein Traceable author statement Ref.3. Source: ProtInc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2 Ref.5

Chain

2 – 340

339

DnaJ homolog subfamily B member 1

PRO_0000071016

Regions

Domain

2 – 70

69

J

Experimental info

Sequence conflict

68

1

G → L in CAA44287. Ref.1

Sequence conflict

150

1

R → C in CAA44287. Ref.1

Sequence conflict

183

1

M → T in CAA44287. Ref.1

Sequence conflict

320

1

V → A in CAA44287. Ref.1

Secondary structure

1

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340

Helix Strand Turn

Details...

Helix

6 – 9

4

Helix

17 – 29

13

Turn

33 – 35

3

Helix

41 – 54

14

Helix

58 – 66

9

Helix

69 – 71

3

Beta strand

166 – 170

5

Helix

172 – 177

6

Beta strand

179 – 190

12

Turn

191 – 193

3

Beta strand

197 – 208

12

Beta strand

217 – 220

4

Beta strand

228 – 230

3

Beta strand

235 – 241

7

Beta strand

248 – 250

3

Beta strand

253 – 261

9

Helix

262 – 267

6

Beta strand

269 – 274

6

Beta strand

280 – 285

6

Beta strand

294 – 297

4

Beta strand

305 – 307

3

Beta strand

314 – 321

8

Helix

328 – 337

10

Sequences

	Sequence		Length	Mass (Da)
	P25685 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 17545098B0C196DF	FASTA	340	38,044
	10 20 30 40 50 60 MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK 70 80 90 100 110 120 REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN 130 140 150 160 170 180 GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT 190 200 210 220 230 240 KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK 250 260 270 280 290 300 DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE 310 320 330 340 GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human homologue of the Escherichia coli DnaJ heat-shock protein." Raabe T., Manley J.L. Nucleic Acids Res. 19:6645-6645(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ." Ohtsuka K. Biochem. Biophys. Res. Commun. 197:235-240(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-48. Tissue: Placenta.
[3]	"Genomic cloning of a human heat shock protein 40 (Hsp40) gene (HSPF1) and its chromosomal localization to 19p13.2." Hata M., Okumura K., Seto M., Ohtsuka K. Genomics 38:446-449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Lung.
[5]	"Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells." Hattori H., Liu Y.-C., Tohnai I., Ueda M., Kaneda T., Kobayashi T., Tanabe K., Ohtsuka K. Cell Struct. Funct. 17:77-86(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-49, INDUCTION, SUBCELLULAR LOCATION.
[6]	"The cellular inhibitor of the PKR protein kinase, P58(IPK), is an influenza virus-activated co-chaperone that modulates heat shock protein 70 activity." Melville M.W., Tan S.-L., Wambach M., Song J., Morimoto R.I., Katze M.G. J. Biol. Chem. 274:3797-3803(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DNAJC3.
[7]	"Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones." Zhong X.Y., Ding J.H., Adams J.A., Ghosh G., Fu X.D. Genes Dev. 23:482-495(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SRPK1.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Nuclear magnetic resonance solution structure of the human Hsp40 (HDJ-1) J-domain." Qian Y.Q., Patel D., Hartl F.-U., McColl D.J. J. Mol. Biol. 260:224-235(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 1-76.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X62421 mRNA. Translation: CAA44287.1. Frameshift.
D49547 mRNA. Translation: BAA08495.1.
D85429 Genomic DNA. Translation: BAA12819.1.
BC002352 mRNA. Translation: AAH02352.1.
BC019827 mRNA. Translation: AAH19827.1.

IPI

IPI00015947.

PIR

JN0912.
S20062.

RefSeq

NP_006136.1. NM_006145.1.

UniGene

Hs.515210.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HDJ	NMR	-	A	1-76	[»]
2QLD	X-ray	2.70	A	158-340	[»]
3AGX	X-ray	1.85	A/B	161-340	[»]
3AGY	X-ray	1.85	A/B	161-340	[»]
3AGZ	X-ray	2.51	A/B	151-340	[»]

ProteinModelPortal

P25685.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-41180N.

IntAct

P25685. 19 interactions.

MINT

MINT-204558.

STRING

9606.ENSP00000254322.

PTM databases

PhosphoSite

P25685.

Polymorphism databases

DMDM

1706473.

2D gel databases

REPRODUCTION-2DPAGE

IPI00015947.

Proteomic databases

PaxDb

P25685.

PeptideAtlas

P25685.

PRIDE

P25685.

Protocols and materials databases

DNASU

3337.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000254322; ENSP00000254322; ENSG00000132002.

GeneID

3337.

KEGG

hsa:3337.

UCSC

uc002myz.1. human.

Organism-specific databases

CTD

3337.

GeneCards

GC19M014625.

H-InvDB

HIX0014838.

HGNC

HGNC:5270. DNAJB1.

HPA

CAB017450.

MIM

604572. gene.

neXtProt

NX_P25685.

PharmGKB

PA27412.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG2214.

HOGENOM

HOG000226718.

HOVERGEN

HBG066727.

InParanoid

P25685.

KO

K09507.

OMA

PERIPVS.

OrthoDB

EOG4PVP00.

PhylomeDB

P25685.

Gene expression databases

ArrayExpress

P25685.

Bgee

P25685.

CleanEx

HS_DNAJB1.

Genevestigator

P25685.

GermOnline

ENSG00000132002. Homo sapiens.

Family and domain databases

Gene3D

1.10.287.110. 1 hit.

InterPro

IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]

Pfam

PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]

PRINTS

PR00625. JDOMAIN.

SMART

SM00271. DnaJ. 1 hit.
[Graphical view]

SUPFAM

SSF46565. DnaJ_N. 1 hit.
SSF49493. HSP40_DnaJ_pep. 2 hits.

PROSITE

PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

DNAJB1. human.

EvolutionaryTrace

P25685.

GenomeRNAi

3337.

NextBio

13208.

SOURCE

Search...

Entry information

Entry name

DNJB1_HUMAN

Accession

Primary (citable) accession number: P25685

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 141 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.