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P25685 (DNJB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 149. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DnaJ homolog subfamily B member 1
Alternative name(s):
DnaJ protein homolog 1
Heat shock 40 kDa protein 1
Short name=HSP40
Short name=Heat shock protein 40
Human DnaJ protein 1
Short name=hDj-1
Gene names
Name:DNAJB1
Synonyms:DNAJ1, HDJ1, HSPF1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.

Subunit structure

Interacts with DNAJC3. Interacts with SRPK1. Ref.6 Ref.7

Subcellular location

Cytoplasm. Nucleus. Nucleusnucleolus. Note: Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. Ref.5

Induction

By heat shock. Ref.5

Sequence similarities

Contains 1 J domain.

Sequence caution

The sequence CAA44287.1 differs from that shown. Reason: Frameshift at positions 11, 28, 81 and 136.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Phlda1Q623922EBI-357034,EBI-309727From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2 Ref.5
Chain2 – 340339DnaJ homolog subfamily B member 1
PRO_0000071016

Regions

Domain2 – 7069J

Experimental info

Sequence conflict681G → L in CAA44287. Ref.1
Sequence conflict1501R → C in CAA44287. Ref.1
Sequence conflict1831M → T in CAA44287. Ref.1
Sequence conflict3201V → A in CAA44287. Ref.1

Secondary structure

............................................. 340
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P25685 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 17545098B0C196DF

FASTA34038,044
        10         20         30         40         50         60 
MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK 

        70         80         90        100        110        120 
REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN 

       130        140        150        160        170        180 
GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT 

       190        200        210        220        230        240 
KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK 

       250        260        270        280        290        300 
DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE 

       310        320        330        340 
GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI 

« Hide

References

« Hide 'large scale' references
[1]"A human homologue of the Escherichia coli DnaJ heat-shock protein."
Raabe T., Manley J.L.
Nucleic Acids Res. 19:6645-6645(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ."
Ohtsuka K.
Biochem. Biophys. Res. Commun. 197:235-240(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-48.
Tissue: Placenta.
[3]"Genomic cloning of a human heat shock protein 40 (Hsp40) gene (HSPF1) and its chromosomal localization to 19p13.2."
Hata M., Okumura K., Seto M., Ohtsuka K.
Genomics 38:446-449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Placenta.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Lung.
[5]"Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells."
Hattori H., Liu Y.-C., Tohnai I., Ueda M., Kaneda T., Kobayashi T., Tanabe K., Ohtsuka K.
Cell Struct. Funct. 17:77-86(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-49, INDUCTION, SUBCELLULAR LOCATION.
[6]"The cellular inhibitor of the PKR protein kinase, P58(IPK), is an influenza virus-activated co-chaperone that modulates heat shock protein 70 activity."
Melville M.W., Tan S.-L., Wambach M., Song J., Morimoto R.I., Katze M.G.
J. Biol. Chem. 274:3797-3803(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DNAJC3.
[7]"Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones."
Zhong X.Y., Ding J.H., Adams J.A., Ghosh G., Fu X.D.
Genes Dev. 23:482-495(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRPK1.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Nuclear magnetic resonance solution structure of the human Hsp40 (HDJ-1) J-domain."
Qian Y.Q., Patel D., Hartl F.-U., McColl D.J.
J. Mol. Biol. 260:224-235(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 1-76.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62421 mRNA. Translation: CAA44287.1. Frameshift.
D49547 mRNA. Translation: BAA08495.1.
D85429 Genomic DNA. Translation: BAA12819.1.
BC002352 mRNA. Translation: AAH02352.1.
BC019827 mRNA. Translation: AAH19827.1.
PIRJN0912.
S20062.
RefSeqNP_006136.1. NM_006145.1.
UniGeneHs.515210.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HDJNMR-A1-76[»]
2QLDX-ray2.70A158-340[»]
3AGXX-ray1.85A/B161-340[»]
3AGYX-ray1.85A/B161-340[»]
3AGZX-ray2.51A/B151-340[»]
ProteinModelPortalP25685.
SMRP25685. Positions 1-340.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109569. 53 interactions.
DIPDIP-41180N.
IntActP25685. 25 interactions.
MINTMINT-204558.
STRING9606.ENSP00000254322.

PTM databases

PhosphoSiteP25685.

Polymorphism databases

DMDM1706473.

2D gel databases

REPRODUCTION-2DPAGEIPI00015947.

Proteomic databases

PaxDbP25685.
PeptideAtlasP25685.
PRIDEP25685.

Protocols and materials databases

DNASU3337.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000254322; ENSP00000254322; ENSG00000132002.
GeneID3337.
KEGGhsa:3337.
UCSCuc002myz.1. human.

Organism-specific databases

CTD3337.
GeneCardsGC19M014625.
H-InvDBHIX0014838.
HGNCHGNC:5270. DNAJB1.
HPACAB017450.
MIM604572. gene.
neXtProtNX_P25685.
PharmGKBPA27412.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2214.
HOGENOMHOG000226718.
HOVERGENHBG066727.
InParanoidP25685.
KOK09507.
OMAHSIFKRD.
OrthoDBEOG7TF79F.
PhylomeDBP25685.
TreeFamTF105141.

Gene expression databases

ArrayExpressP25685.
BgeeP25685.
CleanExHS_DNAJB1.
GenevestigatorP25685.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
InterProIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDNAJB1. human.
EvolutionaryTraceP25685.
GeneWikiDNAJB1.
GenomeRNAi3337.
NextBio13208.
PROP25685.
SOURCESearch...

Entry information

Entry nameDNJB1_HUMAN
AccessionPrimary (citable) accession number: P25685
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 149 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM