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Protein

DnaJ homolog subfamily B member 1

Gene

DNAJB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.

GO - Molecular functioni

  • ATPase activator activity Source: BHF-UCL
  • ATPase binding Source: BHF-UCL
  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • chaperone binding Source: UniProtKB
  • Hsp70 protein binding Source: BHF-UCL
  • unfolded protein binding Source: UniProtKB

GO - Biological processi

  • chaperone cofactor-dependent protein refolding Source: UniProtKB
  • chaperone mediated protein folding requiring cofactor Source: Ensembl
  • negative regulation of inclusion body assembly Source: UniProtKB
  • positive regulation of ATPase activity Source: BHF-UCL
  • regulation of cellular response to heat Source: Reactome
  • response to unfolded protein Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Enzyme and pathway databases

ReactomeiR-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-3371568. Attenuation phase.
R-HSA-3371571. HSF1-dependent transactivation.
R-HSA-5687128. MAPK6/MAPK4 signaling.
SIGNORiP25685.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 1
Alternative name(s):
DnaJ protein homolog 1
Heat shock 40 kDa protein 1
Short name:
HSP40
Short name:
Heat shock protein 40
Human DnaJ protein 1
Short name:
hDj-1
Gene namesi
Name:DNAJB1
Synonyms:DNAJ1, HDJ1, HSPF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:5270. DNAJB1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication
  • Nucleusnucleolus 1 Publication

  • Note: Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasm Source: BHF-UCL
  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

MalaCardsiDNAJB1.
Orphaneti401920. Fibrolamellar hepatocellular carcinoma.
PharmGKBiPA27412.

Polymorphism and mutation databases

BioMutaiDNAJB1.
DMDMi1706473.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 340339DnaJ homolog subfamily B member 1PRO_0000071016Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei307 – 3071PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP25685.
PaxDbiP25685.
PeptideAtlasiP25685.
PRIDEiP25685.

2D gel databases

REPRODUCTION-2DPAGEIPI00015947.

PTM databases

iPTMnetiP25685.
PhosphoSiteiP25685.

Expressioni

Inductioni

By heat shock.1 Publication

Gene expression databases

BgeeiENSG00000132002.
CleanExiHS_DNAJB1.
ExpressionAtlasiP25685. baseline and differential.
GenevisibleiP25685. HS.

Organism-specific databases

HPAiCAB017450.
HPA063247.

Interactioni

Subunit structurei

Interacts with DNAJC3. Interacts with SRPK1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Phlda1Q623922EBI-357034,EBI-309727From a different organism.
TERF1P542742EBI-357034,EBI-710997

GO - Molecular functioni

  • ATPase binding Source: BHF-UCL
  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • chaperone binding Source: UniProtKB
  • Hsp70 protein binding Source: BHF-UCL
  • unfolded protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi109569. 99 interactions.
DIPiDIP-41180N.
IntActiP25685. 44 interactions.
MINTiMINT-204558.
STRINGi9606.ENSP00000254322.

Structurei

Secondary structure

1
340
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 95Combined sources
Helixi17 – 3115Combined sources
Turni33 – 353Combined sources
Helixi41 – 5515Combined sources
Helixi58 – 7013Combined sources
Beta strandi166 – 1705Combined sources
Helixi172 – 1776Combined sources
Beta strandi179 – 19012Combined sources
Turni191 – 1933Combined sources
Beta strandi197 – 20812Combined sources
Beta strandi217 – 2204Combined sources
Beta strandi228 – 2303Combined sources
Beta strandi235 – 2417Combined sources
Beta strandi248 – 2503Combined sources
Beta strandi253 – 2619Combined sources
Helixi262 – 2676Combined sources
Beta strandi269 – 2746Combined sources
Beta strandi280 – 2856Combined sources
Beta strandi294 – 2974Combined sources
Beta strandi305 – 3073Combined sources
Beta strandi314 – 3218Combined sources
Helixi328 – 33710Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HDJNMR-A1-76[»]
2QLDX-ray2.70A158-340[»]
3AGXX-ray1.85A/B161-340[»]
3AGYX-ray1.85A/B161-340[»]
3AGZX-ray2.51A/B151-340[»]
4WB7X-ray1.90A/B2-70[»]
ProteinModelPortaliP25685.
SMRiP25685. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25685.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7069JPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiP25685.
KOiK09507.
OMAiGAFTNMN.
OrthoDBiEOG091G0DLS.
PhylomeDBiP25685.
TreeFamiTF105141.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P25685-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE
60 70 80 90 100
AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA
110 120 130 140 150
MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR
160 170 180 190 200
SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE
210 220 230 240 250
DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD
260 270 280 290 300
GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE
310 320 330 340
GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI
Length:340
Mass (Da):38,044
Last modified:January 23, 2007 - v4
Checksum:i17545098B0C196DF
GO
Isoform 2 (identifier: P25685-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Note: No experimental confirmation available.
Show »
Length:240
Mass (Da):27,016
Checksum:i760FFA819B48174B
GO

Sequence cautioni

The sequence CAA44287 differs from that shown. Reason: Frameshift at positions 11, 28, 81 and 136. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681G → L in CAA44287 (PubMed:1754405).Curated
Sequence conflicti150 – 1501R → C in CAA44287 (PubMed:1754405).Curated
Sequence conflicti183 – 1831M → T in CAA44287 (PubMed:1754405).Curated
Sequence conflicti320 – 3201V → A in CAA44287 (PubMed:1754405).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 100100Missing in isoform 2. 1 PublicationVSP_056414Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62421 mRNA. Translation: CAA44287.1. Frameshift.
D49547 mRNA. Translation: BAA08495.1.
D85429 Genomic DNA. Translation: BAA12819.1.
AK301817 mRNA. Translation: BAG63264.1.
AC009004 Genomic DNA. No translation available.
AC012318 Genomic DNA. No translation available.
BC002352 mRNA. Translation: AAH02352.1.
BC019827 mRNA. Translation: AAH19827.1.
CCDSiCCDS12312.1. [P25685-1]
CCDS74295.1. [P25685-2]
PIRiJN0912.
S20062.
RefSeqiNP_001287843.1. NM_001300914.1. [P25685-2]
NP_001300893.1. NM_001313964.1. [P25685-2]
NP_006136.1. NM_006145.2. [P25685-1]
XP_006722796.1. XM_006722733.1. [P25685-2]
XP_006722797.1. XM_006722734.3. [P25685-2]
XP_011526258.1. XM_011527956.2. [P25685-2]
UniGeneiHs.515210.

Genome annotation databases

EnsembliENST00000254322; ENSP00000254322; ENSG00000132002. [P25685-1]
ENST00000396969; ENSP00000444212; ENSG00000132002. [P25685-2]
GeneIDi3337.
KEGGihsa:3337.
UCSCiuc010xnr.2. human. [P25685-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62421 mRNA. Translation: CAA44287.1. Frameshift.
D49547 mRNA. Translation: BAA08495.1.
D85429 Genomic DNA. Translation: BAA12819.1.
AK301817 mRNA. Translation: BAG63264.1.
AC009004 Genomic DNA. No translation available.
AC012318 Genomic DNA. No translation available.
BC002352 mRNA. Translation: AAH02352.1.
BC019827 mRNA. Translation: AAH19827.1.
CCDSiCCDS12312.1. [P25685-1]
CCDS74295.1. [P25685-2]
PIRiJN0912.
S20062.
RefSeqiNP_001287843.1. NM_001300914.1. [P25685-2]
NP_001300893.1. NM_001313964.1. [P25685-2]
NP_006136.1. NM_006145.2. [P25685-1]
XP_006722796.1. XM_006722733.1. [P25685-2]
XP_006722797.1. XM_006722734.3. [P25685-2]
XP_011526258.1. XM_011527956.2. [P25685-2]
UniGeneiHs.515210.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HDJNMR-A1-76[»]
2QLDX-ray2.70A158-340[»]
3AGXX-ray1.85A/B161-340[»]
3AGYX-ray1.85A/B161-340[»]
3AGZX-ray2.51A/B151-340[»]
4WB7X-ray1.90A/B2-70[»]
ProteinModelPortaliP25685.
SMRiP25685. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109569. 99 interactions.
DIPiDIP-41180N.
IntActiP25685. 44 interactions.
MINTiMINT-204558.
STRINGi9606.ENSP00000254322.

PTM databases

iPTMnetiP25685.
PhosphoSiteiP25685.

Polymorphism and mutation databases

BioMutaiDNAJB1.
DMDMi1706473.

2D gel databases

REPRODUCTION-2DPAGEIPI00015947.

Proteomic databases

EPDiP25685.
PaxDbiP25685.
PeptideAtlasiP25685.
PRIDEiP25685.

Protocols and materials databases

DNASUi3337.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254322; ENSP00000254322; ENSG00000132002. [P25685-1]
ENST00000396969; ENSP00000444212; ENSG00000132002. [P25685-2]
GeneIDi3337.
KEGGihsa:3337.
UCSCiuc010xnr.2. human. [P25685-1]

Organism-specific databases

CTDi3337.
GeneCardsiDNAJB1.
H-InvDBHIX0014838.
HGNCiHGNC:5270. DNAJB1.
HPAiCAB017450.
HPA063247.
MalaCardsiDNAJB1.
MIMi604572. gene.
neXtProtiNX_P25685.
Orphaneti401920. Fibrolamellar hepatocellular carcinoma.
PharmGKBiPA27412.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiP25685.
KOiK09507.
OMAiGAFTNMN.
OrthoDBiEOG091G0DLS.
PhylomeDBiP25685.
TreeFamiTF105141.

Enzyme and pathway databases

ReactomeiR-HSA-3371453. Regulation of HSF1-mediated heat shock response.
R-HSA-3371568. Attenuation phase.
R-HSA-3371571. HSF1-dependent transactivation.
R-HSA-5687128. MAPK6/MAPK4 signaling.
SIGNORiP25685.

Miscellaneous databases

ChiTaRSiDNAJB1. human.
EvolutionaryTraceiP25685.
GeneWikiiDNAJB1.
GenomeRNAii3337.
PROiP25685.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132002.
CleanExiHS_DNAJB1.
ExpressionAtlasiP25685. baseline and differential.
GenevisibleiP25685. HS.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNJB1_HUMAN
AccessioniPrimary (citable) accession number: P25685
Secondary accession number(s): B4DX52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 175 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.