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P25685

- DNJB1_HUMAN

UniProt

P25685 - DNJB1_HUMAN

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Protein
DnaJ homolog subfamily B member 1
Gene
DNAJB1, DNAJ1, HDJ1, HSPF1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.

GO - Molecular functioni

  1. ATPase activator activity Source: BHF-UCL
  2. ATPase binding Source: BHF-UCL
  3. Hsp70 protein binding Source: BHF-UCL
  4. chaperone binding Source: UniProt
  5. protein binding Source: IntAct
  6. unfolded protein binding Source: UniProt

GO - Biological processi

  1. chaperone cofactor-dependent protein refolding Source: UniProtKB
  2. chaperone mediated protein folding requiring cofactor Source: Ensembl
  3. negative regulation of inclusion body assembly Source: UniProt
  4. positive regulation of ATPase activity Source: BHF-UCL
  5. response to unfolded protein Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Enzyme and pathway databases

ReactomeiREACT_200624. Attenuation phase.
REACT_200775. HSF1-dependent transactivation.
REACT_200780. Regulation of HSF1-mediated heat shock response.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 1
Alternative name(s):
DnaJ protein homolog 1
Heat shock 40 kDa protein 1
Short name:
HSP40
Short name:
Heat shock protein 40
Human DnaJ protein 1
Short name:
hDj-1
Gene namesi
Name:DNAJB1
Synonyms:DNAJ1, HDJ1, HSPF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:5270. DNAJB1.

Subcellular locationi

Cytoplasm. Nucleus. Nucleusnucleolus
Note: Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProt
  2. extracellular vesicular exosome Source: UniProt
  3. nucleolus Source: UniProtKB-SubCell
  4. nucleus Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27412.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 340339DnaJ homolog subfamily B member 1
PRO_0000071016Add
BLAST

Proteomic databases

MaxQBiP25685.
PaxDbiP25685.
PeptideAtlasiP25685.
PRIDEiP25685.

2D gel databases

REPRODUCTION-2DPAGEIPI00015947.

PTM databases

PhosphoSiteiP25685.

Expressioni

Inductioni

By heat shock.1 Publication

Gene expression databases

ArrayExpressiP25685.
BgeeiP25685.
CleanExiHS_DNAJB1.
GenevestigatoriP25685.

Organism-specific databases

HPAiCAB017450.

Interactioni

Subunit structurei

Interacts with DNAJC3. Interacts with SRPK1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Phlda1Q623922EBI-357034,EBI-309727From a different organism.

Protein-protein interaction databases

BioGridi109569. 55 interactions.
DIPiDIP-41180N.
IntActiP25685. 25 interactions.
MINTiMINT-204558.
STRINGi9606.ENSP00000254322.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 94
Helixi17 – 2913
Turni33 – 353
Helixi41 – 5414
Helixi58 – 669
Helixi69 – 713
Beta strandi166 – 1705
Helixi172 – 1776
Beta strandi179 – 19012
Turni191 – 1933
Beta strandi197 – 20812
Beta strandi217 – 2204
Beta strandi228 – 2303
Beta strandi235 – 2417
Beta strandi248 – 2503
Beta strandi253 – 2619
Helixi262 – 2676
Beta strandi269 – 2746
Beta strandi280 – 2856
Beta strandi294 – 2974
Beta strandi305 – 3073
Beta strandi314 – 3218
Helixi328 – 33710

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HDJNMR-A1-76[»]
2QLDX-ray2.70A158-340[»]
3AGXX-ray1.85A/B161-340[»]
3AGYX-ray1.85A/B161-340[»]
3AGZX-ray2.51A/B151-340[»]
ProteinModelPortaliP25685.
SMRiP25685. Positions 1-340.

Miscellaneous databases

EvolutionaryTraceiP25685.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7069J
Add
BLAST

Sequence similaritiesi

Contains 1 J domain.

Phylogenomic databases

eggNOGiCOG2214.
HOGENOMiHOG000226718.
HOVERGENiHBG066727.
InParanoidiP25685.
KOiK09507.
OMAiNPFDNFF.
OrthoDBiEOG7TF79F.
PhylomeDBiP25685.
TreeFamiTF105141.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25685-1 [UniParc]FASTAAdd to Basket

« Hide

MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE    50
AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA 100
MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR 150
SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE 200
DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD 250
GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE 300
GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI 340
Length:340
Mass (Da):38,044
Last modified:January 23, 2007 - v4
Checksum:i17545098B0C196DF
GO

Sequence cautioni

The sequence CAA44287.1 differs from that shown. Reason: Frameshift at positions 11, 28, 81 and 136.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681G → L in CAA44287. 1 Publication
Sequence conflicti150 – 1501R → C in CAA44287. 1 Publication
Sequence conflicti183 – 1831M → T in CAA44287. 1 Publication
Sequence conflicti320 – 3201V → A in CAA44287. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62421 mRNA. Translation: CAA44287.1. Frameshift.
D49547 mRNA. Translation: BAA08495.1.
D85429 Genomic DNA. Translation: BAA12819.1.
BC002352 mRNA. Translation: AAH02352.1.
BC019827 mRNA. Translation: AAH19827.1.
CCDSiCCDS12312.1.
PIRiJN0912.
S20062.
RefSeqiNP_006136.1. NM_006145.1.
UniGeneiHs.515210.

Genome annotation databases

EnsembliENST00000254322; ENSP00000254322; ENSG00000132002.
GeneIDi3337.
KEGGihsa:3337.
UCSCiuc002myz.1. human.

Polymorphism databases

DMDMi1706473.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62421 mRNA. Translation: CAA44287.1 . Frameshift.
D49547 mRNA. Translation: BAA08495.1 .
D85429 Genomic DNA. Translation: BAA12819.1 .
BC002352 mRNA. Translation: AAH02352.1 .
BC019827 mRNA. Translation: AAH19827.1 .
CCDSi CCDS12312.1.
PIRi JN0912.
S20062.
RefSeqi NP_006136.1. NM_006145.1.
UniGenei Hs.515210.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HDJ NMR - A 1-76 [» ]
2QLD X-ray 2.70 A 158-340 [» ]
3AGX X-ray 1.85 A/B 161-340 [» ]
3AGY X-ray 1.85 A/B 161-340 [» ]
3AGZ X-ray 2.51 A/B 151-340 [» ]
ProteinModelPortali P25685.
SMRi P25685. Positions 1-340.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 109569. 55 interactions.
DIPi DIP-41180N.
IntActi P25685. 25 interactions.
MINTi MINT-204558.
STRINGi 9606.ENSP00000254322.

PTM databases

PhosphoSitei P25685.

Polymorphism databases

DMDMi 1706473.

2D gel databases

REPRODUCTION-2DPAGE IPI00015947.

Proteomic databases

MaxQBi P25685.
PaxDbi P25685.
PeptideAtlasi P25685.
PRIDEi P25685.

Protocols and materials databases

DNASUi 3337.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000254322 ; ENSP00000254322 ; ENSG00000132002 .
GeneIDi 3337.
KEGGi hsa:3337.
UCSCi uc002myz.1. human.

Organism-specific databases

CTDi 3337.
GeneCardsi GC19M014625.
H-InvDB HIX0014838.
HGNCi HGNC:5270. DNAJB1.
HPAi CAB017450.
MIMi 604572. gene.
neXtProti NX_P25685.
PharmGKBi PA27412.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG2214.
HOGENOMi HOG000226718.
HOVERGENi HBG066727.
InParanoidi P25685.
KOi K09507.
OMAi NPFDNFF.
OrthoDBi EOG7TF79F.
PhylomeDBi P25685.
TreeFami TF105141.

Enzyme and pathway databases

Reactomei REACT_200624. Attenuation phase.
REACT_200775. HSF1-dependent transactivation.
REACT_200780. Regulation of HSF1-mediated heat shock response.

Miscellaneous databases

ChiTaRSi DNAJB1. human.
EvolutionaryTracei P25685.
GeneWikii DNAJB1.
GenomeRNAii 3337.
NextBioi 13208.
PROi P25685.
SOURCEi Search...

Gene expression databases

ArrayExpressi P25685.
Bgeei P25685.
CleanExi HS_DNAJB1.
Genevestigatori P25685.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
InterProi IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A human homologue of the Escherichia coli DnaJ heat-shock protein."
    Raabe T., Manley J.L.
    Nucleic Acids Res. 19:6645-6645(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  2. "Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ."
    Ohtsuka K.
    Biochem. Biophys. Res. Commun. 197:235-240(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-48.
    Tissue: Placenta.
  3. "Genomic cloning of a human heat shock protein 40 (Hsp40) gene (HSPF1) and its chromosomal localization to 19p13.2."
    Hata M., Okumura K., Seto M., Ohtsuka K.
    Genomics 38:446-449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Placenta.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Lung.
  5. "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells."
    Hattori H., Liu Y.-C., Tohnai I., Ueda M., Kaneda T., Kobayashi T., Tanabe K., Ohtsuka K.
    Cell Struct. Funct. 17:77-86(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-49, INDUCTION, SUBCELLULAR LOCATION.
  6. "The cellular inhibitor of the PKR protein kinase, P58(IPK), is an influenza virus-activated co-chaperone that modulates heat shock protein 70 activity."
    Melville M.W., Tan S.-L., Wambach M., Song J., Morimoto R.I., Katze M.G.
    J. Biol. Chem. 274:3797-3803(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DNAJC3.
  7. "Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones."
    Zhong X.Y., Ding J.H., Adams J.A., Ghosh G., Fu X.D.
    Genes Dev. 23:482-495(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRPK1.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Nuclear magnetic resonance solution structure of the human Hsp40 (HDJ-1) J-domain."
    Qian Y.Q., Patel D., Hartl F.-U., McColl D.J.
    J. Mol. Biol. 260:224-235(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-76.

Entry informationi

Entry nameiDNJB1_HUMAN
AccessioniPrimary (citable) accession number: P25685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 153 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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