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P25665

- METE_ECOLI

UniProt

P25665 - METE_ECOLI

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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

metE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Binds 1 zinc ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi641 – 6411Zinc
Metal bindingi643 – 6431Zinc
Metal bindingi726 – 7261Zinc

GO - Molecular functioni

  1. 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Source: EcoCyc
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:HOMOCYSMET-MONOMER.
ECOL316407:JW3805-MONOMER.
MetaCyc:HOMOCYSMET-MONOMER.
BRENDAi2.1.1.14. 2026.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene namesi
Name:metE
Ordered Locus Names:b3829, JW3805
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10584. metE.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi641 – 6411H → N: Impaired activity, lower affinity for zinc binding. 1 Publication
Mutagenesisi641 – 6411H → Q: Impaired activity, lower affinity for zinc binding. Binds homocysteine 2-4x more weakly than wild-type. 1 Publication
Mutagenesisi643 – 6431C → S: Impaired activity, lower affinity for zinc binding. Binds homocysteine 7x tighter than wild-type. 1 Publication
Mutagenesisi726 – 7261C → S: Impaired activity, lower affinity for zinc binding. Binds homocysteine 2-4x more weakly than wild-type. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 7537525-methyltetrahydropteroyltriglutamate--homocysteine methyltransferasePRO_0000098630Add
BLAST

Proteomic databases

PaxDbiP25665.
PRIDEiP25665.

Expressioni

Gene expression databases

GenevestigatoriP25665.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
uvrDP030182EBI-551247,EBI-559573

Protein-protein interaction databases

DIPiDIP-6847N.
IntActiP25665. 11 interactions.
MINTiMINT-1280694.
STRINGi511145.b3829.

Structurei

3D structure databases

ProteinModelPortaliP25665.
SMRiP25665. Positions 3-751.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati2 – 370369ApproximateAdd
BLAST
Repeati371 – 753383ApproximateAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0620.
HOGENOMiHOG000246221.
InParanoidiP25665.
KOiK00549.
OMAiFKANPAH.
OrthoDBiEOG6FFS3G.
PhylomeDBiP25665.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25665-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTILNHTLGF PRVGLRRELK KAQESYWAGN STREELLAVG RELRARHWDQ
60 70 80 90 100
QKQAGIDLLP VGDFAWYDHV LTTSLLLGNV PARHQNKDGS VDIDTLFRIG
110 120 130 140 150
RGRAPTGEPA AAAEMTKWFN TNYHYMVPEF VKGQQFKLTW TQLLDEVDEA
160 170 180 190 200
LALGHKVKPV LLGPVTWLWL GKVKGEQFDR LSLLNDILPV YQQVLAELAK
210 220 230 240 250
RGIEWVQIDE PALVLELPQA WLDAYKPAYD ALQGQVKLLL TTYFEGVTPN
260 270 280 290 300
LDTITALPVQ GLHVDLVHGK DDVAELHKRL PSDWLLSAGL INGRNVWRAD
310 320 330 340 350
LTEKYAQIKD IVGKRDLWVA SSCSLLHSPI DLSVETRLDA EVKSWFAFAL
360 370 380 390 400
QKCHELALLR DALNSGDTAA LAEWSAPIQA RRHSTRVHNP AVEKRLAAIT
410 420 430 440 450
AQDSQRANVY EVRAEAQRAR FKLPAWPTTT IGSFPQTTEI RTLRLDFKKG
460 470 480 490 500
NLDANNYRTG IAEHIKQAIV EQERLGLDVL VHGEAERNDM VEYFGEHLDG
510 520 530 540 550
FVFTQNGWVQ SYGSRCVKPP IVIGDISRPA PITVEWAKYA QSLTDKPVKG
560 570 580 590 600
MLTGPVTILC WSFPREDVSR ETIAKQIALA LRDEVADLEA AGIGIIQIDE
610 620 630 640 650
PALREGLPLR RSDWDAYLQW GVEAFRINAA VAKDDTQIHT HMCYCEFNDI
660 670 680 690 700
MDSIAALDAD VITIETSRSD MELLESFEEF DYPNEIGPGV YDIHSPNVPS
710 720 730 740 750
VEWIEALLKK AAKRIPAERL WVNPDCGLKT RGWPETRAAL ANMVQAAQNL

RRG
Length:753
Mass (Da):84,674
Last modified:January 23, 2007 - v6
Checksum:iC46BE52F227AFAF1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti363 – 3631L → V in AAA23544. (PubMed:1339288)Curated
Sequence conflicti605 – 6051E → Q in AAA67625. (PubMed:8346018)Curated
Sequence conflicti659 – 6591A → R in AAA67625. (PubMed:8346018)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M87625 Genomic DNA. Translation: AAA23544.1.
M87049 Genomic DNA. Translation: AAA67625.1.
U00096 Genomic DNA. Translation: AAC76832.1.
AP009048 Genomic DNA. Translation: BAE77472.1.
J04155 Genomic DNA. Translation: AAA24160.1.
PIRiF65187. A42863.
RefSeqiNP_418273.1. NC_000913.3.
YP_491613.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76832; AAC76832; b3829.
BAE77472; BAE77472; BAE77472.
GeneIDi12930483.
948323.
KEGGiecj:Y75_p3349.
eco:b3829.
PATRICi32123159. VBIEscCol129921_3945.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M87625 Genomic DNA. Translation: AAA23544.1 .
M87049 Genomic DNA. Translation: AAA67625.1 .
U00096 Genomic DNA. Translation: AAC76832.1 .
AP009048 Genomic DNA. Translation: BAE77472.1 .
J04155 Genomic DNA. Translation: AAA24160.1 .
PIRi F65187. A42863.
RefSeqi NP_418273.1. NC_000913.3.
YP_491613.1. NC_007779.1.

3D structure databases

ProteinModelPortali P25665.
SMRi P25665. Positions 3-751.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-6847N.
IntActi P25665. 11 interactions.
MINTi MINT-1280694.
STRINGi 511145.b3829.

Proteomic databases

PaxDbi P25665.
PRIDEi P25665.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76832 ; AAC76832 ; b3829 .
BAE77472 ; BAE77472 ; BAE77472 .
GeneIDi 12930483.
948323.
KEGGi ecj:Y75_p3349.
eco:b3829.
PATRICi 32123159. VBIEscCol129921_3945.

Organism-specific databases

EchoBASEi EB0579.
EcoGenei EG10584. metE.

Phylogenomic databases

eggNOGi COG0620.
HOGENOMi HOG000246221.
InParanoidi P25665.
KOi K00549.
OMAi FKANPAH.
OrthoDBi EOG6FFS3G.
PhylomeDBi P25665.

Enzyme and pathway databases

UniPathwayi UPA00051 ; UER00082 .
BioCyci EcoCyc:HOMOCYSMET-MONOMER.
ECOL316407:JW3805-MONOMER.
MetaCyc:HOMOCYSMET-MONOMER.
BRENDAi 2.1.1.14. 2026.

Miscellaneous databases

PROi P25665.

Gene expression databases

Genevestigatori P25665.

Family and domain databases

HAMAPi MF_00172. Meth_synth.
InterProi IPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view ]
Pfami PF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsi TIGR01371. met_syn_B12ind. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Comparison of cobalamin-independent and cobalamin-dependent methionine synthases from Escherichia coli: two solutions to the same chemical problem."
    Gonzalez J.C., Banerjee R.V., Huang S., Sumner J.S., Matthews R.G.
    Biochemistry 31:6045-6056(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: K12 / DH5-alpha.
  2. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 604 AND 658.
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Regulation of methionine synthesis in Escherichia coli: effect of the MetR protein on the expression of the metE and metR genes."
    Maxon M.E., Redfield B., Cai X.-Y., Shoeman R., Fujita K., Fisher W., Stauffer G., Weissbach H., Brot N.
    Proc. Natl. Acad. Sci. U.S.A. 86:85-89(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
  7. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-13.
    Strain: K12 / EMG2.
  8. "Cobalamin-independent methionine synthase from Escherichia coli: a zinc metalloenzyme."
    Gonzalez J.C., Peariso K., Penner-Hahn J.E., Matthews R.G.
    Biochemistry 35:12228-12234(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF CYS-726.
  9. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  10. "Identification of the zinc ligands in cobalamin-independent methionine synthase (MetE) from Escherichia coli."
    Zhou Z.S., Peariso K., Penner-Hahn J.E., Matthews R.G.
    Biochemistry 38:15915-15926(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF HIS-641; CYS-643 AND CYS-726.

Entry informationi

Entry nameiMETE_ECOLI
AccessioniPrimary (citable) accession number: P25665
Secondary accession number(s): Q2M8D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 148 of the entry and version 6 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Has an absolute requirement for a polyglutamylated folate as substrate. Its activity depends on phosphate anions and divalent cations.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3