SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P25656

- CDC50_YEAST

UniProt

P25656 - CDC50_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Cell division control protein 50
Gene
CDC50, YCR094W, YCR94W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Required for polarized cell growth.

GO - Biological processi

  1. actin cortical patch localization Source: SGD
  2. endocytosis Source: SGD
  3. intracellular protein transport Source: SGD
  4. phospholipid translocation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29388-MONOMER.

Protein family/group databases

TCDBi8.A.27.1.2. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 50
Gene namesi
Name:CDC50
Ordered Locus Names:YCR094W
ORF Names:YCR94W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR094w.
SGDiS000000690. CDC50.

Subcellular locationi

Late endosome membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4444Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei45 – 6521Helical; Reviewed prediction
Add
BLAST
Topological domaini66 – 331266Lumenal Reviewed prediction
Add
BLAST
Transmembranei332 – 35221Helical; Reviewed prediction
Add
BLAST
Topological domaini353 – 39139Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. late endosome membrane Source: SGD
  3. trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391Cell division control protein 50
PRO_0000207670Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi199 – 1991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi216 – 2161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi237 – 2371N-linked (GlcNAc...) Reviewed prediction
Glycosylationi288 – 2881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi329 – 3291N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP25656.
PaxDbiP25656.

Expressioni

Gene expression databases

GenevestigatoriP25656.

Interactioni

Subunit structurei

Interacts with DRS2. Might function as an essential subunit for this protein.1 Publication

Protein-protein interaction databases

BioGridi31066. 114 interactions.
DIPiDIP-5138N.
IntActiP25656. 4 interactions.
MINTiMINT-492575.
STRINGi4932.YCR094W.

Structurei

3D structure databases

ProteinModelPortaliP25656.

Family & Domainsi

Sequence similaritiesi

Belongs to the CDC50/LEM3 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5035.
GeneTreeiENSGT00390000004660.
HOGENOMiHOG000209056.
OMAiTLELYYI.
OrthoDBiEOG7M3JB3.

Family and domain databases

InterProiIPR005045. DUF284_TM_euk.
[Graphical view]
PANTHERiPTHR10926. PTHR10926. 1 hit.
PfamiPF03381. CDC50. 1 hit.
[Graphical view]
PIRSFiPIRSF015840. DUF284_TM_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P25656-1 [UniParc]FASTAAdd to Basket

« Hide

MVSLFKRGKA PPLTKEGPTS KKPPNTAFRQ QRLKAWQPIL SPQSVLPLLI    50
FVACIFTPIG IGLIVSATKV QDLTIDYSHC DTKASTTAFE DIPKKYIKYH 100
FKSKVENKPQ WRLTENENGE QSCELQFEIP NDIKKSIFIY YKITNFYQNH 150
RRYVQSFDTK QILGEPIKKD DLDTSCSPIR SREDKIIYPC GLIANSMFND 200
TFSQVLSGID DTEDYNLTNK HISWSIDRHR FKTTKYNASD IVPPPNWMKK 250
YPDGYTDENL PDIHTWEEFQ VWMRTAAFPK FYKLTLKNES ASLPKGKYQM 300
NIELNYPISL FGGTKSFVLT TNGAIGGRNM SLGVLYLIVA GLCALFGIIF 350
LVKLIFQPRA MGDHTYLNFD DEENEDYEDV HAENTTLREI L 391
Length:391
Mass (Da):44,982
Last modified:May 1, 1992 - v1
Checksum:i565575CA402C0C91
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42249.1.
BK006937 Genomic DNA. Translation: DAA07563.1.
PIRiS19510.
RefSeqiNP_010018.1. NM_001178800.1.

Genome annotation databases

EnsemblFungiiYCR094W; YCR094W; YCR094W.
GeneIDi850456.
KEGGisce:YCR094W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42249.1 .
BK006937 Genomic DNA. Translation: DAA07563.1 .
PIRi S19510.
RefSeqi NP_010018.1. NM_001178800.1.

3D structure databases

ProteinModelPortali P25656.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31066. 114 interactions.
DIPi DIP-5138N.
IntActi P25656. 4 interactions.
MINTi MINT-492575.
STRINGi 4932.YCR094W.

Protein family/group databases

TCDBi 8.A.27.1.2. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

Proteomic databases

MaxQBi P25656.
PaxDbi P25656.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR094W ; YCR094W ; YCR094W .
GeneIDi 850456.
KEGGi sce:YCR094W.

Organism-specific databases

CYGDi YCR094w.
SGDi S000000690. CDC50.

Phylogenomic databases

eggNOGi COG5035.
GeneTreei ENSGT00390000004660.
HOGENOMi HOG000209056.
OMAi TLELYYI.
OrthoDBi EOG7M3JB3.

Enzyme and pathway databases

BioCyci YEAST:G3O-29388-MONOMER.

Miscellaneous databases

NextBioi 966084.
PROi P25656.

Gene expression databases

Genevestigatori P25656.

Family and domain databases

InterProi IPR005045. DUF284_TM_euk.
[Graphical view ]
PANTHERi PTHR10926. PTHR10926. 1 hit.
Pfami PF03381. CDC50. 1 hit.
[Graphical view ]
PIRSFi PIRSF015840. DUF284_TM_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The cloning and characterization of the CDC50 gene family in Saccharomyces cerevisiae."
    Radji M., Kim J.-M., Togan T., Yoshikawa H., Shirahige K.
    Yeast 18:195-205(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae."
    Misu K., Fujimura-Kamada K., Ueda T., Nakano A., Katoh H., Tanaka K.
    Mol. Biol. Cell 14:730-747(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae."
    Saito K., Fujimura-Kamada K., Furuta N., Kato U., Umeda M., Tanaka K.
    Mol. Biol. Cell 15:3418-3432(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DRS2.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiCDC50_YEAST
AccessioniPrimary (citable) accession number: P25656
Secondary accession number(s): D6VR94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi