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P25656 (CDC50_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell division control protein 50
Gene names
Name:CDC50
Ordered Locus Names:YCR094W
ORF Names:YCR94W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for polarized cell growth.

Subunit structure

Interacts with DRS2. Might function as an essential subunit for this protein. Ref.6

Subcellular location

Late endosome membrane; Multi-pass membrane protein Ref.4.

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CDC50/LEM3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 391391Cell division control protein 50
PRO_0000207670

Regions

Topological domain1 – 4444Cytoplasmic Potential
Transmembrane45 – 6521Helical; Potential
Topological domain66 – 331266Lumenal Potential
Transmembrane332 – 35221Helical; Potential
Topological domain353 – 39139Cytoplasmic Potential

Amino acid modifications

Glycosylation1991N-linked (GlcNAc...) Potential
Glycosylation2161N-linked (GlcNAc...) Potential
Glycosylation2371N-linked (GlcNAc...) Potential
Glycosylation2881N-linked (GlcNAc...) Potential
Glycosylation3291N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P25656 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 565575CA402C0C91

FASTA39144,982
        10         20         30         40         50         60 
MVSLFKRGKA PPLTKEGPTS KKPPNTAFRQ QRLKAWQPIL SPQSVLPLLI FVACIFTPIG 

        70         80         90        100        110        120 
IGLIVSATKV QDLTIDYSHC DTKASTTAFE DIPKKYIKYH FKSKVENKPQ WRLTENENGE 

       130        140        150        160        170        180 
QSCELQFEIP NDIKKSIFIY YKITNFYQNH RRYVQSFDTK QILGEPIKKD DLDTSCSPIR 

       190        200        210        220        230        240 
SREDKIIYPC GLIANSMFND TFSQVLSGID DTEDYNLTNK HISWSIDRHR FKTTKYNASD 

       250        260        270        280        290        300 
IVPPPNWMKK YPDGYTDENL PDIHTWEEFQ VWMRTAAFPK FYKLTLKNES ASLPKGKYQM 

       310        320        330        340        350        360 
NIELNYPISL FGGTKSFVLT TNGAIGGRNM SLGVLYLIVA GLCALFGIIF LVKLIFQPRA 

       370        380        390 
MGDHTYLNFD DEENEDYEDV HAENTTLREI L 

« Hide

References

« Hide 'large scale' references
[1]"The cloning and characterization of the CDC50 gene family in Saccharomyces cerevisiae."
Radji M., Kim J.-M., Togan T., Yoshikawa H., Shirahige K.
Yeast 18:195-205(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae."
Misu K., Fujimura-Kamada K., Ueda T., Nakano A., Katoh H., Tanaka K.
Mol. Biol. Cell 14:730-747(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae."
Saito K., Fujimura-Kamada K., Furuta N., Kato U., Umeda M., Tanaka K.
Mol. Biol. Cell 15:3418-3432(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DRS2.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59720 Genomic DNA. Translation: CAA42249.1.
BK006937 Genomic DNA. Translation: DAA07563.1.
PIRS19510.
RefSeqNP_010018.1. NM_001178800.1.

3D structure databases

ProteinModelPortalP25656.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31066. 114 interactions.
DIPDIP-5138N.
IntActP25656. 4 interactions.
MINTMINT-492575.
STRING4932.YCR094W.

Protein family/group databases

TCDB8.A.27.1.2. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

Proteomic databases

MaxQBP25656.
PaxDbP25656.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR094W; YCR094W; YCR094W.
GeneID850456.
KEGGsce:YCR094W.

Organism-specific databases

CYGDYCR094w.
SGDS000000690. CDC50.

Phylogenomic databases

eggNOGCOG5035.
GeneTreeENSGT00390000004660.
HOGENOMHOG000209056.
OMATLELYYI.
OrthoDBEOG7M3JB3.

Enzyme and pathway databases

BioCycYEAST:G3O-29388-MONOMER.

Gene expression databases

GenevestigatorP25656.

Family and domain databases

InterProIPR005045. DUF284_TM_euk.
[Graphical view]
PANTHERPTHR10926. PTHR10926. 1 hit.
PfamPF03381. CDC50. 1 hit.
[Graphical view]
PIRSFPIRSF015840. DUF284_TM_euk. 1 hit.
ProtoNetSearch...

Other

NextBio966084.
PROP25656.

Entry information

Entry nameCDC50_YEAST
AccessionPrimary (citable) accession number: P25656
Secondary accession number(s): D6VR94
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families