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P25656

- CDC50_YEAST

UniProt

P25656 - CDC50_YEAST

Protein

Cell division control protein 50

Gene

CDC50

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Required for polarized cell growth.

    GO - Biological processi

    1. actin cortical patch localization Source: SGD
    2. endocytosis Source: SGD
    3. intracellular protein transport Source: SGD
    4. phospholipid translocation Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29388-MONOMER.

    Protein family/group databases

    TCDBi8.A.27.1.2. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division control protein 50
    Gene namesi
    Name:CDC50
    Ordered Locus Names:YCR094W
    ORF Names:YCR94W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR094w.
    SGDiS000000690. CDC50.

    Subcellular locationi

    Late endosome membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. late endosome membrane Source: SGD
    3. trans-Golgi network Source: SGD

    Keywords - Cellular componenti

    Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 391391Cell division control protein 50PRO_0000207670Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi237 – 2371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP25656.
    PaxDbiP25656.

    Expressioni

    Gene expression databases

    GenevestigatoriP25656.

    Interactioni

    Subunit structurei

    Interacts with DRS2. Might function as an essential subunit for this protein.1 Publication

    Protein-protein interaction databases

    BioGridi31066. 115 interactions.
    DIPiDIP-5138N.
    IntActiP25656. 4 interactions.
    MINTiMINT-492575.
    STRINGi4932.YCR094W.

    Structurei

    3D structure databases

    ProteinModelPortaliP25656.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4444CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini66 – 331266LumenalSequence AnalysisAdd
    BLAST
    Topological domaini353 – 39139CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei45 – 6521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei332 – 35221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CDC50/LEM3 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5035.
    GeneTreeiENSGT00390000004660.
    HOGENOMiHOG000209056.
    OMAiTLELYYI.
    OrthoDBiEOG7M3JB3.

    Family and domain databases

    InterProiIPR005045. DUF284_TM_euk.
    [Graphical view]
    PANTHERiPTHR10926. PTHR10926. 1 hit.
    PfamiPF03381. CDC50. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015840. DUF284_TM_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P25656-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSLFKRGKA PPLTKEGPTS KKPPNTAFRQ QRLKAWQPIL SPQSVLPLLI    50
    FVACIFTPIG IGLIVSATKV QDLTIDYSHC DTKASTTAFE DIPKKYIKYH 100
    FKSKVENKPQ WRLTENENGE QSCELQFEIP NDIKKSIFIY YKITNFYQNH 150
    RRYVQSFDTK QILGEPIKKD DLDTSCSPIR SREDKIIYPC GLIANSMFND 200
    TFSQVLSGID DTEDYNLTNK HISWSIDRHR FKTTKYNASD IVPPPNWMKK 250
    YPDGYTDENL PDIHTWEEFQ VWMRTAAFPK FYKLTLKNES ASLPKGKYQM 300
    NIELNYPISL FGGTKSFVLT TNGAIGGRNM SLGVLYLIVA GLCALFGIIF 350
    LVKLIFQPRA MGDHTYLNFD DEENEDYEDV HAENTTLREI L 391
    Length:391
    Mass (Da):44,982
    Last modified:May 1, 1992 - v1
    Checksum:i565575CA402C0C91
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42249.1.
    BK006937 Genomic DNA. Translation: DAA07563.1.
    PIRiS19510.
    RefSeqiNP_010018.1. NM_001178800.1.

    Genome annotation databases

    EnsemblFungiiYCR094W; YCR094W; YCR094W.
    GeneIDi850456.
    KEGGisce:YCR094W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42249.1 .
    BK006937 Genomic DNA. Translation: DAA07563.1 .
    PIRi S19510.
    RefSeqi NP_010018.1. NM_001178800.1.

    3D structure databases

    ProteinModelPortali P25656.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31066. 115 interactions.
    DIPi DIP-5138N.
    IntActi P25656. 4 interactions.
    MINTi MINT-492575.
    STRINGi 4932.YCR094W.

    Protein family/group databases

    TCDBi 8.A.27.1.2. the cdc50 p-type atpase lipid flippase subunit (cdc50) family.

    Proteomic databases

    MaxQBi P25656.
    PaxDbi P25656.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR094W ; YCR094W ; YCR094W .
    GeneIDi 850456.
    KEGGi sce:YCR094W.

    Organism-specific databases

    CYGDi YCR094w.
    SGDi S000000690. CDC50.

    Phylogenomic databases

    eggNOGi COG5035.
    GeneTreei ENSGT00390000004660.
    HOGENOMi HOG000209056.
    OMAi TLELYYI.
    OrthoDBi EOG7M3JB3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29388-MONOMER.

    Miscellaneous databases

    NextBioi 966084.
    PROi P25656.

    Gene expression databases

    Genevestigatori P25656.

    Family and domain databases

    InterProi IPR005045. DUF284_TM_euk.
    [Graphical view ]
    PANTHERi PTHR10926. PTHR10926. 1 hit.
    Pfami PF03381. CDC50. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015840. DUF284_TM_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The cloning and characterization of the CDC50 gene family in Saccharomyces cerevisiae."
      Radji M., Kim J.-M., Togan T., Yoshikawa H., Shirahige K.
      Yeast 18:195-205(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Cdc50p, a conserved endosomal membrane protein, controls polarized growth in Saccharomyces cerevisiae."
      Misu K., Fujimura-Kamada K., Ueda T., Nakano A., Katoh H., Tanaka K.
      Mol. Biol. Cell 14:730-747(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae."
      Saito K., Fujimura-Kamada K., Furuta N., Kato U., Umeda M., Tanaka K.
      Mol. Biol. Cell 15:3418-3432(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DRS2.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiCDC50_YEAST
    AccessioniPrimary (citable) accession number: P25656
    Secondary accession number(s): D6VR94
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 589 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3