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Protein

General negative regulator of transcription subunit 1

Gene

CDC39

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

Miscellaneous

Present with 4300 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ATPase activator activity Source: SGD
  • protein complex scaffold activity Source: GO_Central

GO - Biological processi

  • negative regulation of cytoplasmic mRNA processing body assembly Source: SGD
  • negative regulation of translation Source: GO_Central
  • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: SGD
  • nuclear-transcribed mRNA poly(A) tail shortening Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • pseudohyphal growth Source: SGD
  • regulation of cell cycle Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • response to pheromone involved in conjugation with cellular fusion Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD

Keywordsi

Molecular functionActivator, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29387-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 1
Alternative name(s):
Cell division cycle protein 39
Gene namesi
Name:CDC39
Synonyms:NOT1, ROS1
Ordered Locus Names:YCR093W
ORF Names:YCR1151, YCR93W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR093W.
SGDiS000000689. CDC39.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000969551 – 2108General negative regulator of transcription subunit 1Add BLAST2108

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2102PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25655.
PRIDEiP25655.

PTM databases

iPTMnetiP25655.

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with CCR4, POP2, NOT2, NOT4 and NOT5. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31065. 348 interactors.
DIPiDIP-1202N.
IntActiP25655. 44 interactors.
MINTiMINT-383459.
STRINGi4932.YCR093W.

Structurei

Secondary structure

12108
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi194 – 197Combined sources4
Helixi198 – 200Combined sources3
Helixi201 – 207Combined sources7
Helixi234 – 245Combined sources12
Helixi249 – 251Combined sources3
Helixi254 – 263Combined sources10
Helixi275 – 285Combined sources11
Helixi288 – 296Combined sources9
Helixi301 – 312Combined sources12
Turni316 – 319Combined sources4
Helixi323 – 325Combined sources3
Beta strandi334 – 336Combined sources3
Helixi343 – 345Combined sources3
Helixi347 – 358Combined sources12
Turni359 – 362Combined sources4
Helixi367 – 382Combined sources16
Helixi384 – 393Combined sources10
Helixi395 – 402Combined sources8
Helixi405 – 421Combined sources17
Helixi424 – 426Combined sources3
Helixi427 – 433Combined sources7
Helixi437 – 450Combined sources14
Helixi457 – 464Combined sources8
Helixi468 – 474Combined sources7
Helixi479 – 482Combined sources4
Helixi484 – 490Combined sources7
Helixi495 – 502Combined sources8
Turni505 – 507Combined sources3
Helixi508 – 520Combined sources13
Turni530 – 532Combined sources3
Helixi536 – 547Combined sources12
Helixi553 – 569Combined sources17
Helixi571 – 573Combined sources3
Turni574 – 577Combined sources4
Helixi581 – 585Combined sources5
Beta strandi589 – 591Combined sources3
Helixi595 – 609Combined sources15
Helixi615 – 626Combined sources12
Helixi631 – 647Combined sources17
Helixi648 – 653Combined sources6
Helixi656 – 671Combined sources16
Helixi677 – 691Combined sources15
Helixi698 – 709Combined sources12
Helixi710 – 716Combined sources7
Helixi718 – 727Combined sources10
Helixi729 – 733Combined sources5
Helixi735 – 743Combined sources9
Beta strandi765 – 767Combined sources3
Helixi783 – 795Combined sources13
Turni798 – 800Combined sources3
Helixi801 – 811Combined sources11
Helixi814 – 816Combined sources3
Helixi817 – 827Combined sources11
Turni828 – 831Combined sources4
Helixi833 – 835Combined sources3
Helixi836 – 846Combined sources11
Helixi849 – 867Combined sources19
Helixi872 – 874Combined sources3
Helixi877 – 890Combined sources14
Helixi892 – 894Combined sources3
Turni900 – 902Combined sources3
Helixi905 – 914Combined sources10
Helixi918 – 929Combined sources12
Helixi930 – 932Combined sources3
Beta strandi936 – 939Combined sources4
Helixi943 – 958Combined sources16
Helixi963 – 975Combined sources13
Helixi980 – 982Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B89X-ray1.50A755-1000[»]
4B8AX-ray2.40A755-1000[»]
4B8BX-ray2.80A/B151-753[»]
4B8CX-ray3.41B/G/H/I755-1000[»]
4CV5X-ray3.81A/C1071-1282[»]
ProteinModelPortaliP25655.
SMRiP25655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili795 – 813Sequence analysisAdd BLAST19
Coiled coili1021 – 1046Sequence analysisAdd BLAST26

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1009 – 1046Gln-richAdd BLAST38
Compositional biasi1300 – 1329Gln-richAdd BLAST30

Sequence similaritiesi

Belongs to the CNOT1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000014869.
HOGENOMiHOG000057131.
InParanoidiP25655.
KOiK12604.
OMAiPNVFAND.
OrthoDBiEOG092C00SU.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR007196. CCR4-Not_Not1_C.
IPR024557. CCR4-Not_Not1su_DUF3819.
IPR032191. CNOT1_CAF1_bind.
IPR032194. CNOT1_HEAT.
IPR032195. CNOT1_HEAT_N.
IPR032193. CNOT1_TTP_bind.
IPR015943. WD40/YVTN_repeat-like_dom.
PfamiView protein in Pfam
PF16415. CNOT1_CAF1_bind. 1 hit.
PF16418. CNOT1_HEAT. 1 hit.
PF16419. CNOT1_HEAT_N. 1 hit.
PF16417. CNOT1_TTP_bind. 1 hit.
PF12842. DUF3819. 1 hit.
PF04054. Not1. 1 hit.

Sequencei

Sequence statusi: Complete.

P25655-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSATYRDLN TASNLETSKE KQAAQIVIAQ ISLLFTTLNN DNFESVEREI
60 70 80 90 100
RHILDRSSVD IYIKVWERLL TLSSRDILQA GKFLLQENLL HRLLLEFAKD
110 120 130 140 150
LPKKSTDLIE LLKERTFNNQ EFQKQTGITL SLFIDLFDKS ANKDIIESLD
160 170 180 190 200
RSSQINDFKT IKMNHTNYLR NFFLQTTPET LESNLRDLLH SLEGESLNDL
210 220 230 240 250
LALLLSEILS PGSQNLQNDP TRSWLTPPMV LDATNRGNVI ARSISSLQAN
260 270 280 290 300
QINWNRVFNL MSTKYFLSAP LMPTTASLSC LFAALHDGPV IDEFFSCDWK
310 320 330 340 350
VIFKLDLAIQ LHKWSVQNGC FDLLNAEGTR KVSETIPNTK QSLLYLLSIA
360 370 380 390 400
SLNLELFLQR EELSDGPMLA YFQECFFEDF NYAPEYLILA LVKEMKRFVL
410 420 430 440 450
LIENRTVIDE ILITLLIQVH NKSPSSFKDV ISTITDDSKI VDAAKIIINS
460 470 480 490 500
DDAPIANFLK SLLDTGRLDT VINKLPFNEA FKILPCARQI GWEGFDTFLK
510 520 530 540 550
TKVSPSNVDV VLESLEVQTK MTDTNTPFRS LKTFDLFAFH SLIEVLNKCP
560 570 580 590 600
LDVLQLQRFE SLEFSLLIAF PRLINFGFGH DEAILANGDI AGINNDIEKE
610 620 630 640 650
MQNYLQKMYS GELAIKDVIE LLRRLRDSDL PRDQEVFTCI THAVIAESTF
660 670 680 690 700
FQDYPLDALA TTSVLFGSMI LFQLLRGFVL DVAFRIIMRF AKEPPESKMF
710 720 730 740 750
KFAVQAIYAF RIRLAEYPQY CKDLLRDVPA LKSQAQVYQS IVEAATLANA
760 770 780 790 800
PKERSRPVQE MIPLKFFAVD EVSCQINQEG APKDVVEKVL FVLNNVTLAN
810 820 830 840 850
LNNKVDELKK SLTPNYFSWF STYLVTQRAK TEPNYHDLYS KVIVAMGSGL
860 870 880 890 900
LHQFMVNVTL RQLFVLLSTK DEQAIDKKHL KNLASWLGCI TLALNKPIKH
910 920 930 940 950
KNIAFREMLI EAYKENRLEI VVPFVTKILQ RASESKIFKP PNPWTVGILK
960 970 980 990 1000
LLIELNEKAN WKLSLTFEVE VLLKSFNLTT KSLKPSNFIN TPEVIETLSG
1010 1020 1030 1040 1050
ALGSITLEQQ QTEQQRQIIL MQQHQQQMLI YQQRQQQQQQ RQQQQQHHIS
1060 1070 1080 1090 1100
ANTIADQQAA FGGEGSISHD NPFNNLLGST IFVTHPDLKR VFQMALAKSV
1110 1120 1130 1140 1150
REILLEVVEK SSGIAVVTTT KIILKDFATE VDESKLKTAA IIMVRHLAQS
1160 1170 1180 1190 1200
LARATSIEPL KEGIRSTMQS LAPNLMSLSS SPAEELDTAI NENIGIALVL
1210 1220 1230 1240 1250
IEKASMDKST QDLADQLMQA IAIRRYHKER RADQPFITQN TNPYSLSLPE
1260 1270 1280 1290 1300
PLGLKNTGVT PQQFRVYEEF GKNIPNLDVI PFAGLPAHAP PMTQNVGLTQ
1310 1320 1330 1340 1350
PQQQQAQMPT QILTSEQIRA QQQQQQLQKS RLNQPSQSAQ PPGVNVPNPQ
1360 1370 1380 1390 1400
GGIAAVQSDL EQNQRVLVHL MDILVSQIKE NATKNNLAEL GDQNQIKTII
1410 1420 1430 1440 1450
FQILTFIAKS AQKDQLALKV SQAVVNSLFA TSESPLCREV LSLLLEKLCS
1460 1470 1480 1490 1500
LSLVARKDVV WWLVYALDSR KFNVPVIRSL LEVNLIDATE LDNVLVTAMK
1510 1520 1530 1540 1550
NKMENSTEFA MKLIQNTVLS DDPILMRMDF IKTLEHLASS EDENVKKFIK
1560 1570 1580 1590 1600
EFEDTKIMPV RKGTKTTRTE KLYLVFTEWV KLLQRVENND VITTVFIKQL
1610 1620 1630 1640 1650
VEKGVISDTD NLLTFVKSSL ELSVSSFKES DPTDEVFIAI DALGSLIIKL
1660 1670 1680 1690 1700
LILQGFKDDT RRDYINAIFS VIVLVFAKDH SQEGTTFNER PYFRLFSNIL
1710 1720 1730 1740 1750
YEWATIRTHN FVRISDSSTR QELIEFDSVF YNTFSGYLHA LQPFAFPGFS
1760 1770 1780 1790 1800
FAWVTLLSHR MLLPIMLRLP NKIGWEKLML LIIDLFKFLD QYTSKHAVSD
1810 1820 1830 1840 1850
AVSVVYKGTL RVILGISNDM PSFLIENHYE LMNNLPPTYF QLKNVILSAI
1860 1870 1880 1890 1900
PKNMTVPNPY DVDLNMEDIP ACKELPEVFF DPVIDLHSLK KPVDNYLRIP
1910 1920 1930 1940 1950
SNSLLRTILS AIYKDTYDIK KGVGYDFLSV DSKLIRAIVL HVGIEAGIEY
1960 1970 1980 1990 2000
KRTSSNAVFN TKSSYYTLLF NLIQNGSIEM KYQIILSIVE QLRYPNIHTY
2010 2020 2030 2040 2050
WFSFVLMNMF KSDEWNDQKL EVQEIILRNF LKRIIVNKPH TWGVSVFFTQ
2060 2070 2080 2090 2100
LINNNDINLL DLPFVQSVPE IKLILQQLVK YSKKYTTSEQ DDQSATINRR

QTPLQSNA
Length:2,108
Mass (Da):240,279
Last modified:October 5, 2010 - v3
Checksum:i5B2AE15BCF95E009
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti526T → A in CAA49721 (PubMed:8428577).Curated1
Sequence conflicti569A → R in CAA49721 (PubMed:8428577).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70151 Genomic DNA. Translation: CAA49721.1.
X59720 Genomic DNA. Translation: CAA42248.2.
BK006937 Genomic DNA. Translation: DAA07562.1.
PIRiS28417.
RefSeqiNP_010017.2. NM_001178799.1.

Genome annotation databases

EnsemblFungiiCAA42248; CAA42248; CAA42248.
YCR093W; YCR093W; YCR093W.
GeneIDi850455.
KEGGisce:YCR093W.

Similar proteinsi

Entry informationi

Entry nameiNOT1_YEAST
AccessioniPrimary (citable) accession number: P25655
Secondary accession number(s): D6VR93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 5, 2010
Last modified: October 25, 2017
This is version 165 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names