P25653 (FIG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Factor-induced gene 2 protein

Alternative name(s):
Cell wall adhesin FIG2

Gene names

Name:	FIG2
Ordered Locus Names:	YCR089W
ORF Names:	YCR1102, YCR89W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for efficient mating. Plays a role in maintenance of cell wall integrity during mating. Important for mating cell projection shape and conjugation bridge diameter. Plays a role in cell fusion and nuclear migration. Ref.6 Ref.7 Ref.8 Ref.9
Subcellular location	Secreted › cell wall. Membrane; Lipid-anchor › GPI-anchor Potential. Note: Periphery of the mating cells. Localized to the mating projection. Ref.5 Ref.6 Ref.7
Induction	By mating pheromones. By cells of the opposite mating type. Ref.6 Ref.7 Ref.9
Post-translational modification	N-glycosylated.

Ontologies

Keywords
Biological process	Cell adhesion Cell shape Cell wall biogenesis/degradation Conjugation Pheromone response
Cellular component	Cell wall Membrane Secreted
Domain	Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW cell morphogenesis involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.6. Source: UniProtKB cytogamy Inferred from mutant phenotype Ref.8. Source: UniProtKB invasive growth in response to glucose limitation Inferred from mutant phenotype Ref.7. Source: SGD regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-KW fungal-type cell wall Inferred from direct assay Ref.7Ref.6. Source: UniProtKB mating projection Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 1588	1566	Factor-induced gene 2 protein		PRO_0000021264
	Propeptide	1589 – 1609	21	Removed in mature form Potential		PRO_0000372452
Regions
	Compositional bias	30 – 1366	1337	Ser-rich
	Compositional bias	529 – 1554	1026	Thr-rich
Amino acid modifications
	Lipidation	1588	1	GPI-anchor amidated glycine Potential
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	231	1	N-linked (GlcNAc...) Potential
	Glycosylation	298	1	N-linked (GlcNAc...) Potential
	Glycosylation	347	1	N-linked (GlcNAc...) Potential
	Glycosylation	386	1	N-linked (GlcNAc...) Potential
	Glycosylation	426	1	N-linked (GlcNAc...) Potential
	Glycosylation	495	1	N-linked (GlcNAc...) Potential
	Glycosylation	535	1	N-linked (GlcNAc...) Potential
	Glycosylation	661	1	N-linked (GlcNAc...) Potential
	Glycosylation	674	1	N-linked (GlcNAc...) Potential
	Glycosylation	713	1	N-linked (GlcNAc...) Potential
	Glycosylation	889	1	N-linked (GlcNAc...) Potential
	Glycosylation	907	1	N-linked (GlcNAc...) Potential
	Glycosylation	1079	1	N-linked (GlcNAc...) Potential
	Glycosylation	1400	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P25653 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: B9B6E08F98996B54	FASTA	1,609	166,036
	10 20 30 40 50 60 MNSFASLGLI YSVVNLLTRV EAQIVFYQNS STSLPVPTLV STSIADFHES SSTGEVQYSS 70 80 90 100 110 120 SYSYVQPSID SFTSSSFLTS FEAPTETSSS YAVSSSLITS DTFSSYSDIF DEETSSLIST 130 140 150 160 170 180 SAASSEKASS TLSSTAQPHR TSHSSSSFEL PVTAPSSSSL PSSTSLTFTS VNPSQSWTSF 190 200 210 220 230 240 NSEKSSALSS TIDFTSSEIS GSTSPKSLES FDTTGTITSS YSPSPSSKNS NQTSLLSPLE 250 260 270 280 290 300 PLSSSSGDLI LSSTIQATTN DQTSKTIPTL VDATSSLPPT LRSSSMAPTS GSDSISHNFT 310 320 330 340 350 360 SPPSKTSGNY DVLTSNSIDP SLFTTTSEYS STQLSSLNRA SKSETVNFTA SIASTPFGTD 370 380 390 400 410 420 SATSLIDPIS SVGSTASSFV GISTANFSTQ GNSNYVPEST ASGSSQYQDW SSSSLPLSQT 430 440 450 460 470 480 TWVVINTTNT QGSVTSTTSP AYVSTATKTV DGVITEYVTW CPLTQTKSQA IGVSSSISSV 490 500 510 520 530 540 PQASSFSGSS ILSSNSSTLA ASNNVPESTA SGSSQYQDWS SSSLPLSQTT WVVINTTNTQ 550 560 570 580 590 600 GSVTSTTSPA YVSTATKTVD GVITEYVTWC PLTQTKSQAI GISSSTISAT QTSKPSSILT 610 620 630 640 650 660 LGISTLQLSD ATFKGTETIN THLMTESTSI TEPTYFSGTS DSFYLCTSEV NLASSLSSYP 670 680 690 700 710 720 NFSSSEGSTA TITNSTVTFG STSKYPSTSV SNPTEASQHV SSSVNSLTDF TSNSTETIAV 730 740 750 760 770 780 ISNIHKTSSN KDYSLTTTQL KTSGMQTLVL STVTTTVNGA ATEYTTWCPA SSIAYTTSIS 790 800 810 820 830 840 YKTLVLTTEV CSHSECTPTV ITSVTATSST IPLLSTSSST VLSSTVSEGA KNPAASEVTI 850 860 870 880 890 900 NTQVSATSEA TSTSTQVSAT SATATASESS TTSQVSTASE TISTLGTQNF TTTGSLLFPA 910 920 930 940 950 960 LSTEMINTTV VSRKTLIIST EVCSHSKCVP TVITEVVTSK GTPSNGHSSQ TLQTEAVEVT 970 980 990 1000 1010 1020 LSSHQTVTMS TEVCSNSICT PTVITSVQMR STPFPYLTSS TSSSSLASTK KSSLEASSEM 1030 1040 1050 1060 1070 1080 STFSVSTQSL PLAFTSSEKR STTSVSQWSN TVLTNTIMSS SSNVISTNEK PSSTTSPYNF 1090 1100 1110 1120 1130 1140 SSGYSLPSSS TPSQYSLSTA TTTINGIKTV YTTWCPLAEK STVAASSQSS RSVDRFVSSS 1150 1160 1170 1180 1190 1200 KPSSSLSQTS IQYTLSTATT TISGLKTVYT TWCPLTSKST LGATTQTSST AKVRITSASS 1210 1220 1230 1240 1250 1260 ATSTSISLST STESESSSGY LSKGVCSGTE CTQDVPTQSS SPASTLAYSP SVSTSSSSSF 1270 1280 1290 1300 1310 1320 STTTASTLTS THTSVPLLPS SSSISASSPS STSLLSTSLP SPAFTSSTLP TATAVSSSTF 1330 1340 1350 1360 1370 1380 IASSLPLSSK SSLSLSPVSS SILMSQFSSS SSSSSSLASL PSLSISPTVD TVSVLQPTTS 1390 1400 1410 1420 1430 1440 IATLTCTDSQ CQQEVSTICN GSNCDDVTST ATTPPSTVTD TMTCTGSECQ KTTSSSCDGY 1450 1460 1470 1480 1490 1500 SCKVSETYKS SATISACSGE GCQASATSEL NSQYVTMTSV ITPSAITTTS VEVHSTESTI 1510 1520 1530 1540 1550 1560 SITTVKPVTY TSSDTNGELI TITSSSQTVI PSVTTIITRT KVAITSAPKP TTTTYVEQRL 1570 1580 1590 1600 SSSGIATSFV AAASSTWITT PIVSTYAGSA SKFLCSKFFM IMVMVINFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of a 6146 bp fragment of Saccharomyces cerevisiae chromosome III contains two new open reading frames." Wilson C., Grisanti P., Frontali L. Yeast 8:569-575(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Valles G., Volckaerts G. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 745 AND 1036.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins." Van der Vaart J.M., te Biesebeke R., Chapman J.W., Toschka H.Y., Klis F.M., Verrips C.T. Appl. Environ. Microbiol. 63:615-620(1997) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Pheromone-regulated genes required for yeast mating differentiation." Erdman S., Lin L., Malczynski M., Snyder M. J. Cell Biol. 140:461-483(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[7]	"A Saccharomyces gene family involved in invasive growth, cell-cell adhesion, and mating." Guo B., Styles C.A., Feng Q., Fink G.R. Proc. Natl. Acad. Sci. U.S.A. 97:12158-12163(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[8]	"Maintenance of mating cell integrity requires the adhesin Fig2p." Zhang M., Bennett D., Erdman S.E. Eukaryot. Cell 1:811-822(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Role of Fig2p in agglutination in Saccharomyces cerevisiae." Jue C.K., Lipke P.N. Eukaryot. Cell 1:843-845(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[10]	"Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes." Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H. Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59720 Genomic DNA. Translation: CAA42254.2. BK006937 Genomic DNA. Translation: DAA07558.1.
PIR	S25345.
RefSeq	NP_010013.2. NM_001178795.1.
3D structure databases
ProteinModelPortal	P25653.
ModBase	Search...
Protein-protein interaction databases
IntAct	P25653. 1 interaction.
MINT	MINT-2781749.
STRING	4932.YCR089W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCR089W; YCR089W; YCR089W.
GeneID	850451.
KEGG	sce:YCR089W.
Organism-specific databases
CYGD	YCR089w.
SGD	S000000685. FIG2.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00700000104630.
OMA	WVVINTT.
OrthoDB	EOG47WS05.
Gene expression databases
Genevestigator	P25653.
GermOnline	YCR089W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR025928. Flocculin_t3_rpt. [Graphical view]
Pfam	PF13928. Flocculin_t3. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	966069.

Entry information

Entry name

FIG2_YEAST

Accession

Primary (citable) accession number: P25653
Secondary accession number(s): D6VR89

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	March 1, 2004
Last modified:	April 3, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program