P25651 (CSM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Monopolin complex subunit CSM1

Alternative name(s):
Chromosome segregation in meiosis protein 1

Gene names

Name:	CSM1
Synonyms:	SPO86
Ordered Locus Names:	YCR086W
ORF Names:	YCR86W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the monopolin complex which promotes monoorientation during meiosis I, required for chromosome segregation during meiosis. Plays also a mitotic role in DNA replication. Ref.4 Ref.6 Ref.9 Ref.10 Ref.11
Subunit structure	Component of the monopolin complex composed of at least CSM1, LRS4 and MAM1. The complex associates with the kinetochore. Interacts with CDC46, CDC47, GIC1, GIC2, MCM3 and NUR1. Ref.5 Ref.6 Ref.9 Ref.11
Subcellular location	Nucleus › nucleolus. Chromosome › centromere. Note: Transiently released from the nucleolus and localizes to the centromere regions during late pachytene. Ref.5 Ref.6 Ref.7 Ref.9
Miscellaneous	Present with 1920 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Meiosis
Cellular component	Centromere Chromosome Nucleus
Domain	Coiled coil
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	homologous chromosome segregation Inferred from mutant phenotype Ref.6. Source: SGD protein localization to nucleolar rDNA repeats Inferred from mutant phenotype PubMed 19362534. Source: SGD rDNA condensation Inferred from mutant phenotype PubMed 18923139. Source: SGD
Cellular_component	monopolin complex Inferred from direct assay Ref.6. Source: SGD nuclear envelope Inferred from direct assay Ref.5. Source: SGD nucleolus Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-22001,EBI-22001
DSE3	Q08729	3	EBI-22001,EBI-32325
DSN1	P40568	3	EBI-22001,EBI-25398
LRS4	Q04087	16	EBI-22001,EBI-32189
MAM1	P40065	8	EBI-22001,EBI-22643
MCM3	P24279	2	EBI-22001,EBI-10541
MCM5	P29496	2	EBI-22001,EBI-10549
MCM7	P38132	3	EBI-22001,EBI-4300
TOF2	Q02208	4	EBI-22001,EBI-27048

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 190

190

Monopolin complex subunit CSM1

PRO_0000202577

Regions

Coiled coil

31 – 70

40

Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

190

Helix Strand Turn

Details...

Helix

71 – 82

12

Beta strand

83 – 91

9

Beta strand

96 – 102

7

Beta strand

116 – 122

7

Beta strand

131 – 137

7

Beta strand

139 – 141

3

Helix

143 – 150

8

Helix

155 – 158

4

Beta strand

161 – 164

4

Helix

165 – 167

3

Helix

168 – 179

12

Sequences

	Sequence		Length	Mass (Da)
	P25651 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 9650E0DA89B31CC5	FASTA	190	21,751
	10 20 30 40 50 60 MDPLTVYKNS VKQQIDSADL LVANLVNENF VLSEKLDTKA TEIKQLQKQI DSLNAQVKEL 70 80 90 100 110 120 KTQTSQQAEN SEVIKDLYEY LCNVRVHKSY EDDSGLWFDI SQGTHSGGSS DDYSIMDYKL 130 140 150 160 170 180 GFVKGQAQVT EVIYAPVLKQ RSTEELYSLQ SKLPEYLFET LSFPLSSLNQ FYNKIAKSLN 190 KKREKKDETE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"A screen for genes required for meiosis and spore formation based on whole-genome expression." Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E., Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E., Klein F., Knop M., Nasmyth K. Curr. Biol. 11:1001-1009(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"A protein interaction map for cell polarity development." Drees B.L., Sundin B.A., Brazeau E., Caviston J.P., Chen G.-C., Guo W., Kozminski K.G., Lau M.W., Moskow J.J., Tong A., Schenkman L.R., McKenzie A. III, Brennwald P.J., Longtine M., Bi E., Chan C., Novick P., Boone C. , Pringle J.R., Davis T.N., Fields S., Drubin D.G. J. Cell Biol. 154:549-571(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GIC1 AND GIC2, SUBCELLULAR LOCATION.
[6]	"Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I." Rabitsch K.P., Petronczki M., Javerzat J.-P., Genier S., Chwalla B., Schleiffer A., Tanaka T.U., Nasmyth K. Dev. Cell 4:535-548(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE MONOPOLIN COMPLEX.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Saccharomyces cerevisiae CSM1 gene encoding a protein influencing chromosome segregation in meiosis I interacts with elements of the DNA replication complex." Wysocka M., Rytka J., Kurlandzka A. Exp. Cell Res. 294:592-602(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CDC46; CDC47 AND MCM3.
[10]	"A DNA integrity network in the yeast Saccharomyces cerevisiae." Pan X., Ye P., Yuan D.S., Wang X., Bader J.S., Boeke J.D. Cell 124:1069-1081(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Role for perinuclear chromosome tethering in maintenance of genome stability." Mekhail K., Seebacher J., Gygi S.P., Moazed D. Nature 456:667-670(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH NUR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X59720 Genomic DNA. Translation: CAA42261.1.
AY558153 Genomic DNA. Translation: AAS56479.1.
BK006937 Genomic DNA. Translation: DAA07555.1.

PIR

S19501.

RefSeq

NP_010009.1. NM_001178792.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3N4R	X-ray	2.60	A/B/C/D	69-181	[»]
3N4S	X-ray	2.35	A/B/C/D	69-181	[»]
3N4X	X-ray	3.41	A/B/C/D	1-190	[»]
3N7N	X-ray	3.90	A/B/C/D	1-190	[»]
4EMC	X-ray	3.05	A/B	1-190	[»]

ProteinModelPortal

P25651.

SMR

P25651. Positions 13-181.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1283N.

IntAct

P25651. 54 interactions.

MINT

MINT-391333.

STRING

4932.YCR086W.

Proteomic databases

PaxDb

P25651.

PeptideAtlas

P25651.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YCR086W; YCR086W; YCR086W.

GeneID

850447.

KEGG

sce:YCR086W.

Organism-specific databases

CYGD

YCR086w.

SGD

S000000682. CSM1.

Phylogenomic databases

eggNOG

NOG43170.

HOGENOM

HOG000000914.

OMA

MDYKLGF.

OrthoDB

EOG4SJ8QD.

Gene expression databases

Genevestigator

P25651.

GermOnline

YCR086W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR020981. Monopolin_cplx_su-Csm1/Pcs1.
[Graphical view]

Pfam

PF12539. Csm1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P25651.

NextBio

966060.

Entry information

Entry name

CSM1_YEAST

Accession

Primary (citable) accession number: P25651
Secondary accession number(s): D6VR86

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program