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P25646

- PDP2_YEAST

UniProt

P25646 - PDP2_YEAST

Protein

[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial

Gene

PTC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (10 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the dephosphorylation and concomitant reactivation of the E1 alpha subunit (PDA1) of the pyruvate dehydrogenase complex. Required for efficient mitophagy in stationary phase cells.3 Publications

    Catalytic activityi

    [Pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O = [pyruvate dehydrogenase (acetyl-transferring)] + phosphate.

    GO - Molecular functioni

    1. [pyruvate dehydrogenase (lipoamide)] phosphatase activity Source: UniProtKB-EC
    2. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. macroautophagy Source: SGD
    2. mitochondrion degradation Source: SGD
    3. positive regulation of catalytic activity Source: SGD
    4. protein dephosphorylation Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29400-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial (EC:3.1.3.43)
    Short name:
    PDP 2
    Alternative name(s):
    Autophagy-related protein phosphatase 1
    Phosphatase two C protein 6
    Protein phosphatase 2C homolog 6
    Short name:
    PP2C-6
    Protein phosphatase of PDH protein 2
    Pyruvate dehydrogenase complex phosphatase 2
    Short name:
    PDC phosphatase 2
    Gene namesi
    Name:PTC6
    Synonyms:AUP1, PPP2
    Ordered Locus Names:YCR079W
    ORF Names:YCR79C, YCR79W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR079w.
    SGDiS000002133. PTC6.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: SGD
    2. mitochondrial matrix Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Confers rapamycin-sensitivity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3434MitochondrionSequence AnalysisAdd
    BLAST
    Chaini35 – 442408[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrialPRO_0000057788Add
    BLAST

    Proteomic databases

    PaxDbiP25646.

    Expressioni

    Gene expression databases

    GenevestigatoriP25646.

    Interactioni

    Subunit structurei

    Interacts with PRO2.1 Publication

    Protein-protein interaction databases

    BioGridi31053. 72 interactions.
    DIPiDIP-2963N.
    IntActiP25646. 9 interactions.
    MINTiMINT-501031.
    STRINGi4932.YCR079W.

    Structurei

    3D structure databases

    ProteinModelPortaliP25646.
    SMRiP25646. Positions 238-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini108 – 402295PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PP2C-like domain.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000000910.
    OMAiDKNGIAH.
    OrthoDBiEOG7Q8CZ1.

    Family and domain databases

    Gene3Di3.60.40.10. 2 hits.
    InterProiIPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P25646-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLGNAYAYC KPSQNVGLKL DLLRGLPGYV GHATSRINRL ENQDNYSIKM    50
    MRSWPNAYGS ALNCSVFDGH GEKGAQLSQL LADKLCSSLD FPEPSWDKQD 100
    LKKLVQEYAR RFPEGNYWKH KLSTFEKFYN KFIKNCNSKQ ELLLMKEGDS 150
    AILGQNGGRM IFDKMGNIID KIALLTELDR LRLFYGFARF DLDQCCGLGT 200
    AAGSTASSIF LYPYDDPNAP IDEGKDDDSW IISHSGLLKL IVTQVGDSKI 250
    ILCDQDGIAH ALTTTHHINS SRERHRLSID PSRLDPDAFG ETRFLNNFAN 300
    TRSFGDVAGK PYGISSEPDI FSFLVGNTLH LPRSERSKLP FNGDECFLAL 350
    VTDGITNKLA DQEVVDLITS TVNSWGLKKA TPQFVAEETI KFIQAIATKH 400
    SDNATCVVVR LSNWGNWPNV DRTGPQRETK LMNAQSNETK LN 442
    Length:442
    Mass (Da):49,421
    Last modified:October 10, 2003 - v2
    Checksum:i0F3303047F957515
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAC42991.1.
    BK006937 Genomic DNA. Translation: DAA07550.1.
    PIRiS19493.
    RefSeqiNP_010003.2. NM_001180034.1.

    Genome annotation databases

    EnsemblFungiiYCR079W; YCR079W; YCR079W.
    GeneIDi850441.
    KEGGisce:YCR079W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAC42991.1 .
    BK006937 Genomic DNA. Translation: DAA07550.1 .
    PIRi S19493.
    RefSeqi NP_010003.2. NM_001180034.1.

    3D structure databases

    ProteinModelPortali P25646.
    SMRi P25646. Positions 238-414.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31053. 72 interactions.
    DIPi DIP-2963N.
    IntActi P25646. 9 interactions.
    MINTi MINT-501031.
    STRINGi 4932.YCR079W.

    Proteomic databases

    PaxDbi P25646.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR079W ; YCR079W ; YCR079W .
    GeneIDi 850441.
    KEGGi sce:YCR079W.

    Organism-specific databases

    CYGDi YCR079w.
    SGDi S000002133. PTC6.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000000910.
    OMAi DKNGIAH.
    OrthoDBi EOG7Q8CZ1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29400-MONOMER.

    Miscellaneous databases

    NextBioi 966045.

    Gene expression databases

    Genevestigatori P25646.

    Family and domain databases

    Gene3Di 3.60.40.10. 2 hits.
    InterProi IPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Valles G., Volckaerts G.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Maris A.F.
      Submitted (JUN-2002) to UniProtKB
      Cited for: EXPRESSION, INTERACTION WITH PRO2.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The YCR079w gene confers a rapamycin-resistant function and encodes the sixth type 2C protein phosphatase in Saccharomyces cerevisiae."
      Ruan H., Yan Z., Sun H., Jiang L.
      FEMS Yeast Res. 7:209-215(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    7. "Aup1p, a yeast mitochondrial protein phosphatase homolog, is required for efficient stationary phase mitophagy and cell survival."
      Tal R., Winter G., Ecker N., Klionsky D.J., Abeliovich H.
      J. Biol. Chem. 282:5617-5624(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases."
      Gey U., Czupalla C., Hoflack B., Rodel G., Krause-Buchholz U.
      J. Biol. Chem. 283:9759-9767(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPDP2_YEAST
    AccessioniPrimary (citable) accession number: P25646
    Secondary accession number(s): D6VR81, Q8NIL5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: October 10, 2003
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 432 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3