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P25646 (PDP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial

Short name=PDP 2
EC=3.1.3.43
Alternative name(s):
Autophagy-related protein phosphatase 1
Phosphatase two C protein 6
Protein phosphatase 2C homolog 6
Short name=PP2C-6
Protein phosphatase of PDH protein 2
Pyruvate dehydrogenase complex phosphatase 2
Short name=PDC phosphatase 2
Gene names
Name:PTC6
Synonyms:AUP1, PPP2
Ordered Locus Names:YCR079W
ORF Names:YCR79C, YCR79W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the dephosphorylation and concomitant reactivation of the E1 alpha subunit (PDA1) of the pyruvate dehydrogenase complex. Required for efficient mitophagy in stationary phase cells. Ref.6 Ref.7 Ref.8

Catalytic activity

[Pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O = [pyruvate dehydrogenase (acetyl-transferring)] + phosphate.

Subunit structure

Interacts with PRO2. Ref.4

Subcellular location

Mitochondrion intermembrane space. Mitochondrion matrix Ref.7 Ref.8.

Disruption phenotype

Confers rapamycin-sensitivity. Ref.6

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 PP2C-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3434Mitochondrion Potential
Chain35 – 442408[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial
PRO_0000057788

Regions

Domain108 – 402295PP2C-like

Sequences

Sequence LengthMass (Da)Tools
P25646 [UniParc].

Last modified October 10, 2003. Version 2.
Checksum: 0F3303047F957515

FASTA44249,421
        10         20         30         40         50         60 
MRLGNAYAYC KPSQNVGLKL DLLRGLPGYV GHATSRINRL ENQDNYSIKM MRSWPNAYGS 

        70         80         90        100        110        120 
ALNCSVFDGH GEKGAQLSQL LADKLCSSLD FPEPSWDKQD LKKLVQEYAR RFPEGNYWKH 

       130        140        150        160        170        180 
KLSTFEKFYN KFIKNCNSKQ ELLLMKEGDS AILGQNGGRM IFDKMGNIID KIALLTELDR 

       190        200        210        220        230        240 
LRLFYGFARF DLDQCCGLGT AAGSTASSIF LYPYDDPNAP IDEGKDDDSW IISHSGLLKL 

       250        260        270        280        290        300 
IVTQVGDSKI ILCDQDGIAH ALTTTHHINS SRERHRLSID PSRLDPDAFG ETRFLNNFAN 

       310        320        330        340        350        360 
TRSFGDVAGK PYGISSEPDI FSFLVGNTLH LPRSERSKLP FNGDECFLAL VTDGITNKLA 

       370        380        390        400        410        420 
DQEVVDLITS TVNSWGLKKA TPQFVAEETI KFIQAIATKH SDNATCVVVR LSNWGNWPNV 

       430        440 
DRTGPQRETK LMNAQSNETK LN 

« Hide

References

« Hide 'large scale' references
[1]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Valles G., Volckaerts G.
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]Maris A.F.
Submitted (JUN-2002) to UniProtKB
Cited for: EXPRESSION, INTERACTION WITH PRO2.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The YCR079w gene confers a rapamycin-resistant function and encodes the sixth type 2C protein phosphatase in Saccharomyces cerevisiae."
Ruan H., Yan Z., Sun H., Jiang L.
FEMS Yeast Res. 7:209-215(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]"Aup1p, a yeast mitochondrial protein phosphatase homolog, is required for efficient stationary phase mitophagy and cell survival."
Tal R., Winter G., Ecker N., Klionsky D.J., Abeliovich H.
J. Biol. Chem. 282:5617-5624(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases."
Gey U., Czupalla C., Hoflack B., Rodel G., Krause-Buchholz U.
J. Biol. Chem. 283:9759-9767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59720 Genomic DNA. Translation: CAC42991.1.
BK006937 Genomic DNA. Translation: DAA07550.1.
PIRS19493.
RefSeqNP_010003.2. NM_001180034.1.

3D structure databases

ProteinModelPortalP25646.
SMRP25646. Positions 238-414.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31053. 72 interactions.
DIPDIP-2963N.
IntActP25646. 9 interactions.
MINTMINT-501031.
STRING4932.YCR079W.

Proteomic databases

PaxDbP25646.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR079W; YCR079W; YCR079W.
GeneID850441.
KEGGsce:YCR079W.

Organism-specific databases

CYGDYCR079w.
SGDS000002133. PTC6.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000000910.
OMADKNGIAH.
OrthoDBEOG7Q8CZ1.

Enzyme and pathway databases

BioCycYEAST:G3O-29400-MONOMER.

Gene expression databases

GenevestigatorP25646.

Family and domain databases

Gene3D3.60.40.10. 2 hits.
InterProIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 2 hits.
ProtoNetSearch...

Other

NextBio966045.

Entry information

Entry namePDP2_YEAST
AccessionPrimary (citable) accession number: P25646
Secondary accession number(s): D6VR81, Q8NIL5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 10, 2003
Last modified: February 19, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families