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Protein

Putative lipase ATG15

Gene

ATG15

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension.7 Publications

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • phospholipase activity Source: SGD
  • triglyceride lipase activity Source: UniProtKB-EC

GO - Biological processi

  • autophagy Source: UniProtKB
  • membrane disassembly Source: UniProtKB
  • multivesicular body membrane disassembly Source: SGD
  • neutral lipid catabolic process Source: SGD
  • phosphatidylserine catabolic process Source: SGD
  • piecemeal microautophagy of nucleus Source: SGD
  • vacuolar protein processing Source: UniProtKB

Keywordsi

Molecular functionHydrolase
Biological processAutophagy, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciYEAST:YCR068W-MONOMER.

Protein family/group databases

ESTHERiyeast-cvt17. Lipase_3.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative lipase ATG15 (EC:3.1.1.3)
Alternative name(s):
Autophagy-related protein 15
Cytoplasm to vacuole targeting protein 17
Gene namesi
Name:ATG15
Synonyms:AUT5, CVT17
Ordered Locus Names:YCR068W
ORF Names:YCR68W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR068W.
SGDiS000000664. ATG15.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14Cytoplasmic1 PublicationAdd BLAST14
Transmembranei15 – 35Helical; Signal-anchor for type II membrane proteinAdd BLAST21
Topological domaini36 – 520Lumenal1 PublicationAdd BLAST485

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi332S → A: Loss of function. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000903721 – 520Putative lipase ATG15Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi202N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi208N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylated.3 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP25641.

Interactioni

Subunit structurei

Binds to both phosphatidylinositol (PI) and phosphatidylinositol 3,5-bisphosphate (PIP2).

Protein-protein interaction databases

BioGridi31044. 303 interactors.
DIPiDIP-5001N.
IntActiP25641. 7 interactors.
MINTiMINT-535113.
STRINGi4932.YCR068W.

Structurei

3D structure databases

ProteinModelPortaliP25641.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000034133.
InParanoidiP25641.
KOiK17900.
OMAiIGTCNGA.
OrthoDBiEOG092C284S.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiView protein in InterPro
IPR029058. AB_hydrolase.
IPR002921. Fungal_lipase-like.
PfamiView protein in Pfam
PF01764. Lipase_3. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiView protein in PROSITE
PS00120. LIPASE_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

P25641-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHKSPSRKR FASPLHLGCI LTLTVLCLIA YYFALPDYLS VGKSSSRGAM
60 70 80 90 100
DQKSDGTFRL KSIYRHGVGA NHRLHQRLEV TPEVISAAGM LYQETTTQGQ
110 120 130 140 150
DFEDQEPLWT TNAEYATTNP FDFEFELRRM PLLMKRMKER DPEFIESYIY
160 170 180 190 200
GETYMTEEEE HAMWIDDDIV APNITDRGTV VSLALMSSNA YVRIPQTGDW
210 220 230 240 250
RNVTEPWNET EPEDFGWDGD GIRGHVFYNE VENIVVLSIK GTSAQGLPGS
260 270 280 290 300
GEDETTGNDK INDNLLFSCC CARVSYLWTT VCDCYVKSYI CDESCLEKEL
310 320 330 340 350
RRKDRFYSAV VDIYKGVLKE YPDAAIWVTG HSLGGALASL LGRTFGLPAV
360 370 380 390 400
AFESPGELLP SKRLHLPFPP GLPSYMEGIW HFGHNADPIF MGTCNGASSS
410 420 430 440 450
CSLVGYAMET ACHTGRVCVY DVVNDKGWSV NMFNHRIHKV IDEVLLGYEQ
460 470 480 490 500
AAKCVEPEPC VDCYNWKFIP SRDWESSSRL ITKTKSHAAP TTTTRTTATT
510 520
TSSSTCVGRN WLGFCTKYEL
Length:520
Mass (Da):58,435
Last modified:October 24, 2003 - v2
Checksum:iB56ABAB72B999019
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42987.1.
BK006937 Genomic DNA. Translation: DAA07540.1.
PIRiS19483.
S74292.
RefSeqiNP_009994.2. NM_001178779.1.

Genome annotation databases

EnsemblFungiiCAC42987; CAC42987; CAC42987.
YCR068W; YCR068W; YCR068W.
GeneIDi850432.
KEGGisce:YCR068W.

Similar proteinsi

Entry informationi

Entry nameiATG15_YEAST
AccessioniPrimary (citable) accession number: P25641
Secondary accession number(s): D6VR71, Q8NIL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 24, 2003
Last modified: November 22, 2017
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names