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P25641

- ATG15_YEAST

UniProt

P25641 - ATG15_YEAST

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Protein
Putative lipase ATG15
Gene
ATG15, AUT5, CVT17, YCR068W, YCR68W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Lipase which is essential for lysis of subvacuolar cytoplasm to vacuole targeted bodies and intravacuolar autophagic bodies. Involved in the lysis of intravacuolar multivesicular body (MVB) vesicles. The intravacuolar membrane disintegration by ATG15 is critical to life span extension.7 Publications

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei332 – 3321Charge relay system By similarity

GO - Molecular functioni

  1. triglyceride lipase activity Source: UniProtKB-EC

GO - Biological processi

  1. autophagy Source: UniProtKB
  2. lipid catabolic process Source: UniProtKB-KW
  3. membrane disassembly Source: UniProtKB
  4. multivesicular body membrane disassembly Source: SGD
  5. piecemeal microautophagy of nucleus Source: SGD
  6. vacuolar protein processing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Autophagy, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciYEAST:YCR068W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative lipase ATG15 (EC:3.1.1.3)
Alternative name(s):
Autophagy-related protein 15
Cytoplasm to vacuole targeting protein 17
Gene namesi
Name:ATG15
Synonyms:AUT5, CVT17
Ordered Locus Names:YCR068W
ORF Names:YCR68W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR068w.
SGDiS000000664. ATG15.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Endosomemultivesicular body membrane; Single-pass type II membrane protein. Prevacuolar compartment membrane; Single-pass type II membrane protein
Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC).2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414Cytoplasmic1 Publication
Add
BLAST
Transmembranei15 – 3521Helical; Signal-anchor for type II membrane protein
Add
BLAST
Topological domaini36 – 520485Lumenal1 Publication
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB
  4. multivesicular body membrane Source: UniProtKB-SubCell
  5. vacuolar lumen Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi332 – 3321S → A: Loss of function. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 520520Putative lipase ATG15
PRO_0000090372Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi173 – 1731N-linked (GlcNAc...) Reviewed prediction
Glycosylationi202 – 2021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi208 – 2081N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylated.3 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP25641.
PaxDbiP25641.

Expressioni

Gene expression databases

GenevestigatoriP25641.

Interactioni

Subunit structurei

Binds to both phosphatidylinositol (PI) and phosphatidylinositol 3,5-bisphosphate (PIP2).

Protein-protein interaction databases

BioGridi31044. 58 interactions.
DIPiDIP-5001N.
IntActiP25641. 5 interactions.
MINTiMINT-535113.
STRINGi4932.YCR068W.

Structurei

3D structure databases

ProteinModelPortaliP25641.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5153.
HOGENOMiHOG000034133.
KOiK17900.
OMAiYMGVCNG.
OrthoDBiEOG71P2N8.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002921. Lipase_3.
[Graphical view]
PfamiPF01764. Lipase_3. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25641-1 [UniParc]FASTAAdd to Basket

« Hide

MLHKSPSRKR FASPLHLGCI LTLTVLCLIA YYFALPDYLS VGKSSSRGAM    50
DQKSDGTFRL KSIYRHGVGA NHRLHQRLEV TPEVISAAGM LYQETTTQGQ 100
DFEDQEPLWT TNAEYATTNP FDFEFELRRM PLLMKRMKER DPEFIESYIY 150
GETYMTEEEE HAMWIDDDIV APNITDRGTV VSLALMSSNA YVRIPQTGDW 200
RNVTEPWNET EPEDFGWDGD GIRGHVFYNE VENIVVLSIK GTSAQGLPGS 250
GEDETTGNDK INDNLLFSCC CARVSYLWTT VCDCYVKSYI CDESCLEKEL 300
RRKDRFYSAV VDIYKGVLKE YPDAAIWVTG HSLGGALASL LGRTFGLPAV 350
AFESPGELLP SKRLHLPFPP GLPSYMEGIW HFGHNADPIF MGTCNGASSS 400
CSLVGYAMET ACHTGRVCVY DVVNDKGWSV NMFNHRIHKV IDEVLLGYEQ 450
AAKCVEPEPC VDCYNWKFIP SRDWESSSRL ITKTKSHAAP TTTTRTTATT 500
TSSSTCVGRN WLGFCTKYEL 520
Length:520
Mass (Da):58,435
Last modified:October 24, 2003 - v2
Checksum:iB56ABAB72B999019
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAC42987.1.
BK006937 Genomic DNA. Translation: DAA07540.1.
PIRiS19483.
S74292.
RefSeqiNP_009994.2. NM_001178779.1.

Genome annotation databases

EnsemblFungiiYCR068W; YCR068W; YCR068W.
GeneIDi850432.
KEGGisce:YCR068W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAC42987.1 .
BK006937 Genomic DNA. Translation: DAA07540.1 .
PIRi S19483.
S74292.
RefSeqi NP_009994.2. NM_001178779.1.

3D structure databases

ProteinModelPortali P25641.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31044. 58 interactions.
DIPi DIP-5001N.
IntActi P25641. 5 interactions.
MINTi MINT-535113.
STRINGi 4932.YCR068W.

Proteomic databases

MaxQBi P25641.
PaxDbi P25641.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR068W ; YCR068W ; YCR068W .
GeneIDi 850432.
KEGGi sce:YCR068W.

Organism-specific databases

CYGDi YCR068w.
SGDi S000000664. ATG15.

Phylogenomic databases

eggNOGi COG5153.
HOGENOMi HOG000034133.
KOi K17900.
OMAi YMGVCNG.
OrthoDBi EOG71P2N8.

Enzyme and pathway databases

BioCyci YEAST:YCR068W-MONOMER.

Miscellaneous databases

NextBioi 966018.

Gene expression databases

Genevestigatori P25641.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR002921. Lipase_3.
[Graphical view ]
Pfami PF01764. Lipase_3. 1 hit.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00120. LIPASE_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Degradation of lipid vesicles in the yeast vacuole requires function of Cvt17, a putative lipase."
    Teter S.A., Eggerton K.P., Scott S.V., Kim J., Fischer A.M., Klionsky D.J.
    J. Biol. Chem. 276:2083-2087(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, GLYCOSYLATION, MUTAGENESIS OF SER-332.
  2. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Valles G., Volckaerts G.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Aut5/Cvt17p, a putative lipase essential for disintegration of autophagic bodies inside the vacuole."
    Epple U.D., Suriapranata I., Eskelinen E.-L., Thumm M.
    J. Bacteriol. 183:5942-5955(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, MUTAGENESIS OF SER-332.
  6. "Vacuolar localization of oligomeric alpha-mannosidase requires the cytoplasm to vacuole targeting and autophagy pathway components in Saccharomyces cerevisiae."
    Hutchins M.U., Klionsky D.J.
    J. Biol. Chem. 276:20491-20498(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Intravacuolar membrane lysis in Saccharomyces cerevisiae. Does vacuolar targeting of Cvt17/Aut5p affect its function?"
    Epple U.D., Eskelinen E.-L., Thumm M.
    J. Biol. Chem. 278:7810-7821(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, GLYCOSYLATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A life-span extending form of autophagy employs the vacuole-vacuole fusion machinery."
    Tang F., Watkins J.W., Bermudez M., Gray R., Gaban A., Portie K., Grace S., Kleve M., Craciun G.
    Autophagy 4:874-886(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Starvation induced cell death in autophagy-defective yeast mutants is caused by mitochondria dysfunction."
    Suzuki S.W., Onodera J., Ohsumi Y.
    PLoS ONE 6:E17412-E17412(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "An overexpression screen in Saccharomyces cerevisiae identifies novel genes that affect endocytic protein trafficking."
    Arlt H., Perz A., Ungermann C.
    Traffic 12:1592-1603(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Profiling lipid-protein interactions using nonquenched fluorescent liposomal nanovesicles and proteome microarrays."
    Lu K.Y., Tao S.C., Yang T.C., Ho Y.H., Lee C.H., Lin C.C., Juan H.F., Huang H.C., Yang C.Y., Chen M.S., Lin Y.Y., Lu J.Y., Zhu H., Chen C.S.
    Mol. Cell. Proteomics 11:1177-1190(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHOINOSITIDES-BINDING.

Entry informationi

Entry nameiATG15_YEAST
AccessioniPrimary (citable) accession number: P25641
Secondary accession number(s): D6VR71, Q8NIL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 24, 2003
Last modified: June 11, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi