Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P25635

- PWP2_YEAST

UniProt

P25635 - PWP2_YEAST

Protein

Periodic tryptophan protein 2

Gene

PWP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. snoRNA binding Source: SGD

    GO - Biological processi

    1. cytokinesis, completion of separation Source: SGD
    2. endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    3. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    4. endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    5. establishment of cell polarity Source: SGD
    6. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29363-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periodic tryptophan protein 2
    Alternative name(s):
    U three protein 1
    U3 small nucleolar RNA-associated protein 1
    Short name:
    U3 snoRNA-associated protein 1
    Gene namesi
    Name:PWP2
    Synonyms:UTP1
    Ordered Locus Names:YCR057C
    ORF Names:YCR55C/YCR57C/YCR58C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR057c.
    SGDiS000000653. PWP2.

    Subcellular locationi

    Nucleusnucleolus 1 Publication

    GO - Cellular componenti

    1. 90S preribosome Source: SGD
    2. cytoplasm Source: SGD
    3. nucleolus Source: SGD
    4. Pwp2p-containing subcomplex of 90S preribosome Source: SGD
    5. small-subunit processome Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 923923Periodic tryptophan protein 2PRO_0000051179Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei225 – 2251Phosphoserine1 Publication
    Modified residuei232 – 2321Phosphoserine3 Publications
    Modified residuei651 – 6511Phosphoserine3 Publications
    Modified residuei664 – 6641Phosphoserine1 Publication
    Modified residuei912 – 9121Phosphoserine3 Publications
    Modified residuei913 – 9131Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP25635.
    PaxDbiP25635.
    PeptideAtlasiP25635.

    Expressioni

    Gene expression databases

    GenevestigatoriP25635.

    Interactioni

    Subunit structurei

    Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DIP2Q122206EBI-14332,EBI-5896
    UTP21Q060787EBI-14332,EBI-359

    Protein-protein interaction databases

    BioGridi31035. 118 interactions.
    DIPiDIP-1868N.
    IntActiP25635. 115 interactions.
    MINTiMINT-392472.

    Structurei

    3D structure databases

    ProteinModelPortaliP25635.
    SMRiP25635. Positions 19-723.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati12 – 5241WD 1Add
    BLAST
    Repeati53 – 9341WD 2Add
    BLAST
    Repeati94 – 13239WD 3Add
    BLAST
    Repeati144 – 18340WD 4Add
    BLAST
    Repeati189 – 22840WD 5Add
    BLAST
    Repeati258 – 29740WD 6Add
    BLAST
    Repeati300 – 34041WD 7Add
    BLAST
    Repeati343 – 38240WD 8Add
    BLAST
    Repeati385 – 42440WD 9Add
    BLAST
    Repeati428 – 47043WD 10Add
    BLAST
    Repeati471 – 51040WD 11Add
    BLAST
    Repeati513 – 55240WD 12Add
    BLAST
    Repeati575 – 61440WD 13Add
    BLAST
    Repeati676 – 71439WD 14Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi226 – 24015Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi862 – 92362Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat PWP2 family.Curated
    Contains 14 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    GeneTreeiENSGT00550000074981.
    HOGENOMiHOG000160363.
    KOiK14558.
    OMAiIRAWDLI.
    OrthoDBiEOG7BCNM6.

    Family and domain databases

    Gene3Di2.130.10.10. 4 hits.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR027145. PWP2.
    IPR011047. Quinonprotein_ADH-like_supfam.
    IPR007148. SSU_processome_Utp12.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PANTHERiPTHR19858. PTHR19858. 1 hit.
    PfamiPF04003. Utp12. 1 hit.
    PF00400. WD40. 6 hits.
    [Graphical view]
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 13 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 3 hits.
    SSF50998. SSF50998. 2 hits.
    PROSITEiPS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25635-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSDFKFSNL LGTVYRQGNI TFSDDGKQLL SPVGNRVSVF DLINNKSFTF    50
    EYEHRKNIAA IDLNKQGTLL ISIDEDGRAI LVNFKARNVL HHFNFKEKCS 100
    AVKFSPDGRL FALASGRFLQ IWKTPDVNKD RQFAPFVRHR VHAGHFQDIT 150
    SLTWSQDSRF ILTTSKDLSA KIWSVDSEEK NLAATTFNGH RDYVMGAFFS 200
    HDQEKIYTVS KDGAVFVWEF TKRPSDDDDN ESEDDDKQEE VDISKYSWRI 250
    TKKHFFYANQ AKVKCVTFHP ATRLLAVGFT SGEFRLYDLP DFTLIQQLSM 300
    GQNPVNTVSV NQTGEWLAFG SSKLGQLLVY EWQSESYILK QQGHFDSTNS 350
    LAYSPDGSRV VTASEDGKIK VWDITSGFCL ATFEEHTSSV TAVQFAKRGQ 400
    VMFSSSLDGT VRAWDLIRYR NFRTFTGTER IQFNCLAVDP SGEVVCAGSL 450
    DNFDIHVWSV QTGQLLDALS GHEGPVSCLS FSQENSVLAS ASWDKTIRIW 500
    SIFGRSQQVE PIEVYSDVLA LSMRPDGKEV AVSTLKGQIS IFNIEDAKQV 550
    GNIDCRKDII SGRFNQDRFT AKNSERSKFF TTIHYSFDGM AIVAGGNNNS 600
    ICLYDVPNEV LLKRFIVSRN MALNGTLEFL NSKKMTEAGS LDLIDDAGEN 650
    SDLEDRIDNS LPGSQRGGDL STRKMRPEVR VTSVQFSPTA NAFAAASTEG 700
    LLIYSTNDTI LFDPFDLDVD VTPHSTVEAL REKQFLNALV MAFRLNEEYL 750
    INKVYEAIPI KEIPLVASNI PAIYLPRILK FIGDFAIESQ HIEFNLIWIK 800
    ALLSASGGYI NEHKYLFSTA MRSIQRFIVR VAKEVVNTTT DNKYTYRFLV 850
    STDGSMEDGA ADDDEVLLKD DADEDNEENE ENDVVMESDD EEGWIGFNGK 900
    DNKLPLSNEN DSSDEEENEK ELP 923
    Length:923
    Mass (Da):103,983
    Last modified:February 1, 1995 - v2
    Checksum:iCBEE4720F81378B6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78964 Genomic DNA. Translation: CAA55558.1.
    X59720 Genomic DNA. Translation: CAA42286.1.
    BK006937 Genomic DNA. Translation: DAA07532.1.
    PIRiS44226.
    RefSeqiNP_009984.1. NM_001178769.1.

    Genome annotation databases

    EnsemblFungiiYCR057C; YCR057C; YCR057C.
    GeneIDi850422.
    KEGGisce:YCR057C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78964 Genomic DNA. Translation: CAA55558.1 .
    X59720 Genomic DNA. Translation: CAA42286.1 .
    BK006937 Genomic DNA. Translation: DAA07532.1 .
    PIRi S44226.
    RefSeqi NP_009984.1. NM_001178769.1.

    3D structure databases

    ProteinModelPortali P25635.
    SMRi P25635. Positions 19-723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31035. 118 interactions.
    DIPi DIP-1868N.
    IntActi P25635. 115 interactions.
    MINTi MINT-392472.

    Proteomic databases

    MaxQBi P25635.
    PaxDbi P25635.
    PeptideAtlasi P25635.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR057C ; YCR057C ; YCR057C .
    GeneIDi 850422.
    KEGGi sce:YCR057C.

    Organism-specific databases

    CYGDi YCR057c.
    SGDi S000000653. PWP2.

    Phylogenomic databases

    eggNOGi COG2319.
    GeneTreei ENSGT00550000074981.
    HOGENOMi HOG000160363.
    KOi K14558.
    OMAi IRAWDLI.
    OrthoDBi EOG7BCNM6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29363-MONOMER.

    Miscellaneous databases

    NextBioi 965992.
    PROi P25635.

    Gene expression databases

    Genevestigatori P25635.

    Family and domain databases

    Gene3Di 2.130.10.10. 4 hits.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR027145. PWP2.
    IPR011047. Quinonprotein_ADH-like_supfam.
    IPR007148. SSU_processome_Utp12.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    PANTHERi PTHR19858. PTHR19858. 1 hit.
    Pfami PF04003. Utp12. 1 hit.
    PF00400. WD40. 6 hits.
    [Graphical view ]
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 13 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 3 hits.
    SSF50998. SSF50998. 2 hits.
    PROSITEi PS00678. WD_REPEATS_1. 3 hits.
    PS50082. WD_REPEATS_2. 5 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PWP2, a member of the WD-repeat family of proteins, is an essential Saccharomyces cerevisiae gene involved in cell separation."
      Shafaatian R., Payton M.A., Reid J.D.
      Mol. Gen. Genet. 252:101-114(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 204511 / S288c / AB972.
    2. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Gromadka R.
      Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-651; SER-912 AND SER-913, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-651; SER-664; SER-912 AND SER-913, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; SER-232; SER-651; SER-912 AND SER-913, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPWP2_YEAST
    AccessioniPrimary (citable) accession number: P25635
    Secondary accession number(s): D6VR63, P25633, P25636
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 146 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 16700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3