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Protein

Periodic tryptophan protein 2

Gene

PWP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA.2 Publications

GO - Molecular functioni

  • snoRNA binding Source: SGD

GO - Biological processi

  • cell separation after cytokinesis Source: SGD
  • endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • establishment of cell polarity Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal small subunit assembly Source: GO_Central
  • rRNA methylation Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29363-MONOMER.
ReactomeiR-SCE-6790901. rRNA modification in the nucleus and cytosol.
R-SCE-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Periodic tryptophan protein 2
Alternative name(s):
U three protein 1
U3 small nucleolar RNA-associated protein 1
Short name:
U3 snoRNA-associated protein 1
Gene namesi
Name:PWP2
Synonyms:UTP1
Ordered Locus Names:YCR057C
ORF Names:YCR55C/YCR57C/YCR58C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR057C.
SGDiS000000653. PWP2.

Subcellular locationi

GO - Cellular componenti

  • 90S preribosome Source: SGD
  • cytoplasm Source: SGD
  • nucleolus Source: SGD
  • nucleoplasm Source: Reactome
  • Pwp2p-containing subcomplex of 90S preribosome Source: SGD
  • small-subunit processome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511791 – 923Periodic tryptophan protein 2Add BLAST923

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei225PhosphoserineCombined sources1
Modified residuei232PhosphoserineCombined sources1
Modified residuei651PhosphoserineCombined sources1
Modified residuei664PhosphoserineCombined sources1
Modified residuei912PhosphoserineCombined sources1
Modified residuei913PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25635.
PRIDEiP25635.

PTM databases

iPTMnetiP25635.

Interactioni

Subunit structurei

Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DIP2Q122206EBI-14332,EBI-5896
UTP18P403629EBI-14332,EBI-4534
UTP21Q060789EBI-14332,EBI-359

Protein-protein interaction databases

BioGridi31035. 122 interactors.
DIPiDIP-1868N.
IntActiP25635. 118 interactors.
MINTiMINT-392472.

Structurei

3D structure databases

ProteinModelPortaliP25635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati12 – 52WD 1Add BLAST41
Repeati53 – 93WD 2Add BLAST41
Repeati94 – 132WD 3Add BLAST39
Repeati144 – 183WD 4Add BLAST40
Repeati189 – 228WD 5Add BLAST40
Repeati258 – 297WD 6Add BLAST40
Repeati300 – 340WD 7Add BLAST41
Repeati343 – 382WD 8Add BLAST40
Repeati385 – 424WD 9Add BLAST40
Repeati428 – 470WD 10Add BLAST43
Repeati471 – 510WD 11Add BLAST40
Repeati513 – 552WD 12Add BLAST40
Repeati575 – 614WD 13Add BLAST40
Repeati676 – 714WD 14Add BLAST39

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi226 – 240Asp/Glu-rich (acidic)Add BLAST15
Compositional biasi862 – 923Asp/Glu-rich (acidic)Add BLAST62

Sequence similaritiesi

Belongs to the WD repeat PWP2 family.Curated
Contains 14 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00550000074981.
HOGENOMiHOG000160363.
InParanoidiP25635.
KOiK14558.
OMAiEMPAFSN.
OrthoDBiEOG092C0E9D.

Family and domain databases

Gene3Di2.130.10.10. 4 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR027145. PWP2.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR007148. SSU_processome_Utp12.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19858. PTHR19858. 1 hit.
PfamiPF04003. Utp12. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 13 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
SSF50998. SSF50998. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSDFKFSNL LGTVYRQGNI TFSDDGKQLL SPVGNRVSVF DLINNKSFTF
60 70 80 90 100
EYEHRKNIAA IDLNKQGTLL ISIDEDGRAI LVNFKARNVL HHFNFKEKCS
110 120 130 140 150
AVKFSPDGRL FALASGRFLQ IWKTPDVNKD RQFAPFVRHR VHAGHFQDIT
160 170 180 190 200
SLTWSQDSRF ILTTSKDLSA KIWSVDSEEK NLAATTFNGH RDYVMGAFFS
210 220 230 240 250
HDQEKIYTVS KDGAVFVWEF TKRPSDDDDN ESEDDDKQEE VDISKYSWRI
260 270 280 290 300
TKKHFFYANQ AKVKCVTFHP ATRLLAVGFT SGEFRLYDLP DFTLIQQLSM
310 320 330 340 350
GQNPVNTVSV NQTGEWLAFG SSKLGQLLVY EWQSESYILK QQGHFDSTNS
360 370 380 390 400
LAYSPDGSRV VTASEDGKIK VWDITSGFCL ATFEEHTSSV TAVQFAKRGQ
410 420 430 440 450
VMFSSSLDGT VRAWDLIRYR NFRTFTGTER IQFNCLAVDP SGEVVCAGSL
460 470 480 490 500
DNFDIHVWSV QTGQLLDALS GHEGPVSCLS FSQENSVLAS ASWDKTIRIW
510 520 530 540 550
SIFGRSQQVE PIEVYSDVLA LSMRPDGKEV AVSTLKGQIS IFNIEDAKQV
560 570 580 590 600
GNIDCRKDII SGRFNQDRFT AKNSERSKFF TTIHYSFDGM AIVAGGNNNS
610 620 630 640 650
ICLYDVPNEV LLKRFIVSRN MALNGTLEFL NSKKMTEAGS LDLIDDAGEN
660 670 680 690 700
SDLEDRIDNS LPGSQRGGDL STRKMRPEVR VTSVQFSPTA NAFAAASTEG
710 720 730 740 750
LLIYSTNDTI LFDPFDLDVD VTPHSTVEAL REKQFLNALV MAFRLNEEYL
760 770 780 790 800
INKVYEAIPI KEIPLVASNI PAIYLPRILK FIGDFAIESQ HIEFNLIWIK
810 820 830 840 850
ALLSASGGYI NEHKYLFSTA MRSIQRFIVR VAKEVVNTTT DNKYTYRFLV
860 870 880 890 900
STDGSMEDGA ADDDEVLLKD DADEDNEENE ENDVVMESDD EEGWIGFNGK
910 920
DNKLPLSNEN DSSDEEENEK ELP
Length:923
Mass (Da):103,983
Last modified:February 1, 1995 - v2
Checksum:iCBEE4720F81378B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78964 Genomic DNA. Translation: CAA55558.1.
X59720 Genomic DNA. Translation: CAA42286.1.
BK006937 Genomic DNA. Translation: DAA07532.1.
PIRiS44226.
RefSeqiNP_009984.1. NM_001178769.1.

Genome annotation databases

EnsemblFungiiCAA42286; CAA42286; CAA42286.
YCR057C; YCR057C; YCR057C.
GeneIDi850422.
KEGGisce:YCR057C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78964 Genomic DNA. Translation: CAA55558.1.
X59720 Genomic DNA. Translation: CAA42286.1.
BK006937 Genomic DNA. Translation: DAA07532.1.
PIRiS44226.
RefSeqiNP_009984.1. NM_001178769.1.

3D structure databases

ProteinModelPortaliP25635.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31035. 122 interactors.
DIPiDIP-1868N.
IntActiP25635. 118 interactors.
MINTiMINT-392472.

PTM databases

iPTMnetiP25635.

Proteomic databases

MaxQBiP25635.
PRIDEiP25635.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42286; CAA42286; CAA42286.
YCR057C; YCR057C; YCR057C.
GeneIDi850422.
KEGGisce:YCR057C.

Organism-specific databases

EuPathDBiFungiDB:YCR057C.
SGDiS000000653. PWP2.

Phylogenomic databases

GeneTreeiENSGT00550000074981.
HOGENOMiHOG000160363.
InParanoidiP25635.
KOiK14558.
OMAiEMPAFSN.
OrthoDBiEOG092C0E9D.

Enzyme and pathway databases

BioCyciYEAST:G3O-29363-MONOMER.
ReactomeiR-SCE-6790901. rRNA modification in the nucleus and cytosol.
R-SCE-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Miscellaneous databases

PROiP25635.

Family and domain databases

Gene3Di2.130.10.10. 4 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR027145. PWP2.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR007148. SSU_processome_Utp12.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19858. PTHR19858. 1 hit.
PfamiPF04003. Utp12. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 13 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
SSF50998. SSF50998. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPWP2_YEAST
AccessioniPrimary (citable) accession number: P25635
Secondary accession number(s): D6VR63, P25633, P25636
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 16700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.