P25635 (PWP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periodic tryptophan protein 2

Alternative name(s):
U three protein 1
U3 small nucleolar RNA-associated protein 1
Short name=U3 snoRNA-associated protein 1

Gene names

Name:	PWP2
Synonyms:	UTP1
Ordered Locus Names:	YCR057C
ORF Names:	YCR55C/YCR57C/YCR58C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA. Ref.1 Ref.5
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.5
Subcellular location	Nucleus › nucleolus Ref.5.
Miscellaneous	Present with 16700 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the WD repeat PWP2 family. Contains 14 WD repeats.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat WD repeat
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytokinesis Inferred from mutant phenotype Ref.1. Source: SGD endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD establishment of cell polarity Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	Pwp2p-containing subcomplex of 90S preribosome Inferred from direct assay. Source: SGD cytoplasm Inferred from direct assay Ref.1. Source: SGD small-subunit processome Inferred from direct assay Ref.5. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct snoRNA binding Inferred from physical interaction Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DIP2	Q12220	6	EBI-14332,EBI-5896
UTP13	Q05946	7	EBI-14332,EBI-34702
UTP21	Q06078	7	EBI-14332,EBI-359
YGR210C	P42942	2	EBI-14332,EBI-23482

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 923	923	Periodic tryptophan protein 2		PRO_0000051179
Regions
	Repeat	12 – 52	41	WD 1
	Repeat	53 – 93	41	WD 2
	Repeat	94 – 132	39	WD 3
	Repeat	144 – 183	40	WD 4
	Repeat	189 – 228	40	WD 5
	Repeat	258 – 297	40	WD 6
	Repeat	300 – 340	41	WD 7
	Repeat	343 – 382	40	WD 8
	Repeat	385 – 424	40	WD 9
	Repeat	428 – 470	43	WD 10
	Repeat	471 – 510	40	WD 11
	Repeat	513 – 552	40	WD 12
	Repeat	575 – 614	40	WD 13
	Repeat	676 – 714	39	WD 14
	Compositional bias	226 – 240	15	Asp/Glu-rich (acidic)
	Compositional bias	862 – 923	62	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	232	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Modified residue	651	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Modified residue	660	1	Phosphoserine Ref.9
	Modified residue	664	1	Phosphoserine Ref.9
	Modified residue	912	1	Phosphoserine Ref.7 Ref.9
	Modified residue	913	1	Phosphoserine Ref.7 Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P25635 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: CBEE4720F81378B6	FASTA	923	103,983
	10 20 30 40 50 60 MKSDFKFSNL LGTVYRQGNI TFSDDGKQLL SPVGNRVSVF DLINNKSFTF EYEHRKNIAA 70 80 90 100 110 120 IDLNKQGTLL ISIDEDGRAI LVNFKARNVL HHFNFKEKCS AVKFSPDGRL FALASGRFLQ 130 140 150 160 170 180 IWKTPDVNKD RQFAPFVRHR VHAGHFQDIT SLTWSQDSRF ILTTSKDLSA KIWSVDSEEK 190 200 210 220 230 240 NLAATTFNGH RDYVMGAFFS HDQEKIYTVS KDGAVFVWEF TKRPSDDDDN ESEDDDKQEE 250 260 270 280 290 300 VDISKYSWRI TKKHFFYANQ AKVKCVTFHP ATRLLAVGFT SGEFRLYDLP DFTLIQQLSM 310 320 330 340 350 360 GQNPVNTVSV NQTGEWLAFG SSKLGQLLVY EWQSESYILK QQGHFDSTNS LAYSPDGSRV 370 380 390 400 410 420 VTASEDGKIK VWDITSGFCL ATFEEHTSSV TAVQFAKRGQ VMFSSSLDGT VRAWDLIRYR 430 440 450 460 470 480 NFRTFTGTER IQFNCLAVDP SGEVVCAGSL DNFDIHVWSV QTGQLLDALS GHEGPVSCLS 490 500 510 520 530 540 FSQENSVLAS ASWDKTIRIW SIFGRSQQVE PIEVYSDVLA LSMRPDGKEV AVSTLKGQIS 550 560 570 580 590 600 IFNIEDAKQV GNIDCRKDII SGRFNQDRFT AKNSERSKFF TTIHYSFDGM AIVAGGNNNS 610 620 630 640 650 660 ICLYDVPNEV LLKRFIVSRN MALNGTLEFL NSKKMTEAGS LDLIDDAGEN SDLEDRIDNS 670 680 690 700 710 720 LPGSQRGGDL STRKMRPEVR VTSVQFSPTA NAFAAASTEG LLIYSTNDTI LFDPFDLDVD 730 740 750 760 770 780 VTPHSTVEAL REKQFLNALV MAFRLNEEYL INKVYEAIPI KEIPLVASNI PAIYLPRILK 790 800 810 820 830 840 FIGDFAIESQ HIEFNLIWIK ALLSASGGYI NEHKYLFSTA MRSIQRFIVR VAKEVVNTTT 850 860 870 880 890 900 DNKYTYRFLV STDGSMEDGA ADDDEVLLKD DADEDNEENE ENDVVMESDD EEGWIGFNGK 910 920 DNKLPLSNEN DSSDEEENEK ELP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PWP2, a member of the WD-repeat family of proteins, is an essential Saccharomyces cerevisiae gene involved in cell separation." Shafaatian R., Payton M.A., Reid J.D. Mol. Gen. Genet. 252:101-114(1996) [PubMed: 8804409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 204511 / S288c / AB972.
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Gromadka R. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed: 12068309] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-651; SER-912 AND SER-913, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232 AND SER-651, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-651; SER-660; SER-664; SER-912 AND SER-913, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78964 Genomic DNA. Translation: CAA55558.1. X59720 Genomic DNA. Translation: CAA42286.1. BK006937 Genomic DNA. Translation: DAA07532.1.
PIR	S44226.
RefSeq	NP_009984.1. NM_001178769.1.
3D structure databases
ProteinModelPortal	P25635.
SMR	P25635. Positions 19-613.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1868N.
IntAct	P25635. 118 interactions.
MINT	MINT-392472.
STRING	P25635.
Proteomic databases
PeptideAtlas	P25635.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCR057C; YCR057C; YCR057C.
GeneID	850422.
KEGG	sce:YCR057C.
NMPDR	fig|4932.3.peg.712.
Organism-specific databases
CYGD	YCR057c.
SGD	S000000653. PWP2.
Phylogenomic databases
eggNOG	fuNOG05128.
GeneTree	EFGT00050000000044.
HOGENOM	HBG721153.
OMA	VQFLQKG.
OrthoDB	EOG4350F7.
Gene expression databases
ArrayExpress	P25635.
Genevestigator	P25635.
GermOnline	YCR057C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR011047. Quinonprotein_ADH-like. IPR007148. SSU_processome_Utp12. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 4 hits.
KO	K14558.
Pfam	PF04003. Utp12. 1 hit. PF00400. WD40. 6 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 13 hits. [Graphical view]
SUPFAM	SSF50998. Quin_alc_DH_like. 1 hit. SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 3 hits. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	965992.

Entry information

Entry name

PWP2_YEAST

Accession

Primary (citable) accession number: P25635
Secondary accession number(s): D6VR63, P25633, P25636

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program