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P25635

- PWP2_YEAST

UniProt

P25635 - PWP2_YEAST

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Protein

Periodic tryptophan protein 2

Gene

PWP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA.2 Publications

GO - Molecular functioni

  1. snoRNA binding Source: SGD

GO - Biological processi

  1. cytokinesis, completion of separation Source: SGD
  2. endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  3. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  4. endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  5. establishment of cell polarity Source: SGD
  6. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29363-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periodic tryptophan protein 2
Alternative name(s):
U three protein 1
U3 small nucleolar RNA-associated protein 1
Short name:
U3 snoRNA-associated protein 1
Gene namesi
Name:PWP2
Synonyms:UTP1
Ordered Locus Names:YCR057C
ORF Names:YCR55C/YCR57C/YCR58C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR057c.
SGDiS000000653. PWP2.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. 90S preribosome Source: SGD
  2. cytoplasm Source: SGD
  3. nucleolus Source: SGD
  4. Pwp2p-containing subcomplex of 90S preribosome Source: SGD
  5. small-subunit processome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 923923Periodic tryptophan protein 2PRO_0000051179Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei225 – 2251Phosphoserine1 Publication
Modified residuei232 – 2321Phosphoserine3 Publications
Modified residuei651 – 6511Phosphoserine3 Publications
Modified residuei664 – 6641Phosphoserine1 Publication
Modified residuei912 – 9121Phosphoserine3 Publications
Modified residuei913 – 9131Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25635.
PaxDbiP25635.
PeptideAtlasiP25635.

Expressioni

Gene expression databases

GenevestigatoriP25635.

Interactioni

Subunit structurei

Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DIP2Q122206EBI-14332,EBI-5896
UTP21Q060787EBI-14332,EBI-359

Protein-protein interaction databases

BioGridi31035. 119 interactions.
DIPiDIP-1868N.
IntActiP25635. 115 interactions.
MINTiMINT-392472.

Structurei

3D structure databases

ProteinModelPortaliP25635.
SMRiP25635. Positions 19-723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati12 – 5241WD 1Add
BLAST
Repeati53 – 9341WD 2Add
BLAST
Repeati94 – 13239WD 3Add
BLAST
Repeati144 – 18340WD 4Add
BLAST
Repeati189 – 22840WD 5Add
BLAST
Repeati258 – 29740WD 6Add
BLAST
Repeati300 – 34041WD 7Add
BLAST
Repeati343 – 38240WD 8Add
BLAST
Repeati385 – 42440WD 9Add
BLAST
Repeati428 – 47043WD 10Add
BLAST
Repeati471 – 51040WD 11Add
BLAST
Repeati513 – 55240WD 12Add
BLAST
Repeati575 – 61440WD 13Add
BLAST
Repeati676 – 71439WD 14Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi226 – 24015Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi862 – 92362Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat PWP2 family.Curated
Contains 14 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
GeneTreeiENSGT00550000074981.
HOGENOMiHOG000160363.
InParanoidiP25635.
KOiK14558.
OMAiIRAWDLI.
OrthoDBiEOG7BCNM6.

Family and domain databases

Gene3Di2.130.10.10. 4 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR027145. PWP2.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR007148. SSU_processome_Utp12.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19858. PTHR19858. 1 hit.
PfamiPF04003. Utp12. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 13 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.
SSF50998. SSF50998. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25635-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSDFKFSNL LGTVYRQGNI TFSDDGKQLL SPVGNRVSVF DLINNKSFTF
60 70 80 90 100
EYEHRKNIAA IDLNKQGTLL ISIDEDGRAI LVNFKARNVL HHFNFKEKCS
110 120 130 140 150
AVKFSPDGRL FALASGRFLQ IWKTPDVNKD RQFAPFVRHR VHAGHFQDIT
160 170 180 190 200
SLTWSQDSRF ILTTSKDLSA KIWSVDSEEK NLAATTFNGH RDYVMGAFFS
210 220 230 240 250
HDQEKIYTVS KDGAVFVWEF TKRPSDDDDN ESEDDDKQEE VDISKYSWRI
260 270 280 290 300
TKKHFFYANQ AKVKCVTFHP ATRLLAVGFT SGEFRLYDLP DFTLIQQLSM
310 320 330 340 350
GQNPVNTVSV NQTGEWLAFG SSKLGQLLVY EWQSESYILK QQGHFDSTNS
360 370 380 390 400
LAYSPDGSRV VTASEDGKIK VWDITSGFCL ATFEEHTSSV TAVQFAKRGQ
410 420 430 440 450
VMFSSSLDGT VRAWDLIRYR NFRTFTGTER IQFNCLAVDP SGEVVCAGSL
460 470 480 490 500
DNFDIHVWSV QTGQLLDALS GHEGPVSCLS FSQENSVLAS ASWDKTIRIW
510 520 530 540 550
SIFGRSQQVE PIEVYSDVLA LSMRPDGKEV AVSTLKGQIS IFNIEDAKQV
560 570 580 590 600
GNIDCRKDII SGRFNQDRFT AKNSERSKFF TTIHYSFDGM AIVAGGNNNS
610 620 630 640 650
ICLYDVPNEV LLKRFIVSRN MALNGTLEFL NSKKMTEAGS LDLIDDAGEN
660 670 680 690 700
SDLEDRIDNS LPGSQRGGDL STRKMRPEVR VTSVQFSPTA NAFAAASTEG
710 720 730 740 750
LLIYSTNDTI LFDPFDLDVD VTPHSTVEAL REKQFLNALV MAFRLNEEYL
760 770 780 790 800
INKVYEAIPI KEIPLVASNI PAIYLPRILK FIGDFAIESQ HIEFNLIWIK
810 820 830 840 850
ALLSASGGYI NEHKYLFSTA MRSIQRFIVR VAKEVVNTTT DNKYTYRFLV
860 870 880 890 900
STDGSMEDGA ADDDEVLLKD DADEDNEENE ENDVVMESDD EEGWIGFNGK
910 920
DNKLPLSNEN DSSDEEENEK ELP
Length:923
Mass (Da):103,983
Last modified:February 1, 1995 - v2
Checksum:iCBEE4720F81378B6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78964 Genomic DNA. Translation: CAA55558.1.
X59720 Genomic DNA. Translation: CAA42286.1.
BK006937 Genomic DNA. Translation: DAA07532.1.
PIRiS44226.
RefSeqiNP_009984.1. NM_001178769.1.

Genome annotation databases

EnsemblFungiiYCR057C; YCR057C; YCR057C.
GeneIDi850422.
KEGGisce:YCR057C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78964 Genomic DNA. Translation: CAA55558.1 .
X59720 Genomic DNA. Translation: CAA42286.1 .
BK006937 Genomic DNA. Translation: DAA07532.1 .
PIRi S44226.
RefSeqi NP_009984.1. NM_001178769.1.

3D structure databases

ProteinModelPortali P25635.
SMRi P25635. Positions 19-723.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31035. 119 interactions.
DIPi DIP-1868N.
IntActi P25635. 115 interactions.
MINTi MINT-392472.

Proteomic databases

MaxQBi P25635.
PaxDbi P25635.
PeptideAtlasi P25635.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR057C ; YCR057C ; YCR057C .
GeneIDi 850422.
KEGGi sce:YCR057C.

Organism-specific databases

CYGDi YCR057c.
SGDi S000000653. PWP2.

Phylogenomic databases

eggNOGi COG2319.
GeneTreei ENSGT00550000074981.
HOGENOMi HOG000160363.
InParanoidi P25635.
KOi K14558.
OMAi IRAWDLI.
OrthoDBi EOG7BCNM6.

Enzyme and pathway databases

BioCyci YEAST:G3O-29363-MONOMER.

Miscellaneous databases

NextBioi 965992.
PROi P25635.

Gene expression databases

Genevestigatori P25635.

Family and domain databases

Gene3Di 2.130.10.10. 4 hits.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR027145. PWP2.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR007148. SSU_processome_Utp12.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
PANTHERi PTHR19858. PTHR19858. 1 hit.
Pfami PF04003. Utp12. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view ]
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 13 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 3 hits.
SSF50998. SSF50998. 2 hits.
PROSITEi PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PWP2, a member of the WD-repeat family of proteins, is an essential Saccharomyces cerevisiae gene involved in cell separation."
    Shafaatian R., Payton M.A., Reid J.D.
    Mol. Gen. Genet. 252:101-114(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 204511 / S288c / AB972.
  2. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Gromadka R.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-651; SER-912 AND SER-913, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-651; SER-664; SER-912 AND SER-913, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; SER-232; SER-651; SER-912 AND SER-913, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPWP2_YEAST
AccessioniPrimary (citable) accession number: P25635
Secondary accession number(s): D6VR63, P25633, P25636
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 16700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3