Chromatin structure-remodeling complex protein RSC6 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P25632 (RSC6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chromatin structure-remodeling complex protein RSC6

Alternative name(s):
Remodel the structure of chromatin complex subunit 6

Gene names

Name:	RSC6
Ordered Locus Names:	YCR052W
ORF Names:	YCR52W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and suppresses formamide sensitivity of the RSC8 mutants. Ref.4 Ref.5 Ref.6 Ref.7 Ref.9 Ref.10 Ref.11
Subunit structure	Interacts directly with RSC8. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Ref.5 Ref.11
Subcellular location	Nucleus. Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1. Ref.11
Miscellaneous	Present with 2470 molecules/cell in log phase SD medium.
Sequence similarities	To yeast SNF12.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ATP-dependent chromatin remodeling Inferred from direct assay Ref.6 Ref.9 Ref.4. Source: UniProtKB G1 phase of mitotic cell cycle Inferred from genetic interaction PubMed 17542652. Source: SGD G2/M transition of mitotic cell cycle Inferred from mutant phenotype PubMed 17542652. Source: SGD nucleosome disassembly Inferred from direct assay PubMed 16492771. Source: SGD nucleosome positioning Inferred from direct assay PubMed 21343911. Source: SGD positive regulation of transcription elongation from RNA polymerase II promoter Inferred from direct assay PubMed 17081996. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	RSC complex Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSC58	Q07979	7	EBI-21941,EBI-36549
RSC8	P43609	12	EBI-21941,EBI-23005

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 483

483

Chromatin structure-remodeling complex protein RSC6

PRO_0000097474

Sequences

Sequence

Length

Mass (Da)

Tools

P25632 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 6A9998BDBC4D24B2
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FASTA

483

54,165

        10         20         30         40         50         60 
MVTQTNPVPV TYPTDAYIPT YLPDDKVSNL ADLKKLIEMD SRLDLYLTRR RLDTSINLPT 

        70         80         90        100        110        120 
NTKTKDHPPN KEMLRIYVYN TTESSPRSDS GTPADSGKTT WTLRIEGKLL HESANGKHPF 

       130        140        150        160        170        180 
SEFLEGVAVD FKRLKPLGMG KKRKRDSSLS LPLNLQQPEY NDQDSTMGDN DNGEDEDSAE 

       190        200        210        220        230        240 
AESREEIVDA LEWNYDENNV VEFDGIDIKR QGKDNLRCSI TIQLRGVDGG KVQYSPNLAT 

       250        260        270        280        290        300 
LIGMQTGSVN DAVYSIYKYI LINNLFVTEQ TEAQDGSNDA EDSSNENNNK NGAGDDDGVE 

       310        320        330        340        350        360 
GSTPKDKPEL GEVKLDSLLQ KVLDTNAAHL PLMNVVQTVN KLVSPLPPII LDYTIDLSKD 

       370        380        390        400        410        420 
TTYGATTLDV DVSHILHQPQ PQPNLQKEEE TDAEDTAKLR EITKLALQLN SSAQKYQFFH 

       430        440        450        460        470        480 
ELSLHPRETL THYLWSSKQN ELVLQGDQYF NEDAARTSDI YSNNNNDRSL MGNISLLYSQ 


GRL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"RSC, an essential, abundant chromatin-remodeling complex." Cairns B.R., Lorch Y., Li Y., Zhang M., Lacomis L., Erdjument-Bromage H., Tempst P., Du J., Laurent B.C., Kornberg R.D. Cell 87:1249-1260(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 416-427, FUNCTION, COMPOSITION OF THE RSC COMPLEX.
[5]	"Direct interaction between Rsc6 and Rsc8/Swh3, two proteins that are conserved in SWI/SNF-related complexes." Treich I., Ho L., Carlson M. Nucleic Acids Res. 26:3739-3745(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RSC8.
[6]	"Histone octamer transfer by a chromatin-remodeling complex." Lorch Y., Zhang M., Kornberg R.D. Cell 96:389-392(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[7]	"Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC." Moreira J.M.A., Holmberg S. EMBO J. 18:2836-2844(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[8]	"Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains." Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F. Mol. Cell 4:715-723(1999) [PubMed] [Europe PMC] [Abstract] Cited for: COMPOSITION OF THE RSC COMPLEX.
[9]	"Chromatin remodeling by RSC involves ATP-dependent DNA translocation." Saha A., Wittmeyer J., Cairns B.R. Genes Dev. 16:2120-2134(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[10]	"Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway." Chai B., Hsu J.-M., Du J., Laurent B.C. Genetics 161:575-584(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[11]	"The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation." Hsu J.-M., Huang J., Meluh P.B., Laurent B.C. Mol. Cell. Biol. 23:3202-3215(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59720 Genomic DNA. Translation: CAA42283.1. AY692985 Genomic DNA. Translation: AAT93004.1. BK006937 Genomic DNA. Translation: DAA07529.1.
PIR	S19466.
RefSeq	NP_009981.1. NM_001178766.1.
3D structure databases
ProteinModelPortal	P25632.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4999N.
IntAct	P25632. 48 interactions.
MINT	MINT-493435.
STRING	4932.YCR052W.
Proteomic databases
PaxDb	P25632.
PeptideAtlas	P25632.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCR052W; YCR052W; YCR052W.
GeneID	850419.
KEGG	sce:YCR052W.
Organism-specific databases
CYGD	YCR052w.
SGD	S000000648. RSC6.
Phylogenomic databases
eggNOG	COG5531.
GeneTree	ENSGT00390000017809.
HOGENOM	HOG000000860.
KO	K11760.
OMA	LHESANG.
OrthoDB	EOG4N8VDJ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-29361-MONOMER.
Gene expression databases
Genevestigator	P25632.
GermOnline	YCR052W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003121. SWIB_MDM2_domain. [Graphical view]
SUPFAM	SSF47592. MDM2. 1 hit.
ProtoNet	Search...
Other
NextBio	965983.

Entry information

Entry name

RSC6_YEAST

Accession

Primary (citable) accession number: P25632
Secondary accession number(s): D6VR60

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents