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P25632

- RSC6_YEAST

UniProt

P25632 - RSC6_YEAST

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Protein
Chromatin structure-remodeling complex protein RSC6
Gene
RSC6, YCR052W, YCR52W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and suppresses formamide sensitivity of the RSC8 mutants.7 Publications

GO - Molecular functioni

  1. protein binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. ATP-dependent chromatin remodeling Source: UniProtKB
  3. G2/M transition of mitotic cell cycle Source: SGD
  4. nucleosome disassembly Source: SGD
  5. nucleosome positioning Source: SGD
  6. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29361-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin structure-remodeling complex protein RSC6
Alternative name(s):
Remodel the structure of chromatin complex subunit 6
Gene namesi
Name:RSC6
Ordered Locus Names:YCR052W
ORF Names:YCR52W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR052w.
SGDiS000000648. RSC6.

Subcellular locationi

Nucleus
Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.1 Publication

GO - Cellular componenti

  1. RSC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483Chromatin structure-remodeling complex protein RSC6
PRO_0000097474Add
BLAST

Proteomic databases

MaxQBiP25632.
PaxDbiP25632.
PeptideAtlasiP25632.

Expressioni

Gene expression databases

GenevestigatoriP25632.

Interactioni

Subunit structurei

Interacts directly with RSC8. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RSC58Q079797EBI-21941,EBI-36549
RSC8P4360912EBI-21941,EBI-23005

Protein-protein interaction databases

BioGridi31032. 123 interactions.
DIPiDIP-4999N.
IntActiP25632. 48 interactions.
MINTiMINT-493435.
STRINGi4932.YCR052W.

Structurei

3D structure databases

ProteinModelPortaliP25632.

Family & Domainsi

Sequence similaritiesi

To yeast SNF12.

Phylogenomic databases

eggNOGiCOG5531.
GeneTreeiENSGT00730000114141.
HOGENOMiHOG000000860.
KOiK11760.
OMAiLHESANG.
OrthoDBiEOG7X0VS9.

Family and domain databases

Gene3Di1.10.245.10. 2 hits.
InterProiIPR003121. SWIB_MDM2_domain.
[Graphical view]
SUPFAMiSSF47592. SSF47592. 2 hits.

Sequencei

Sequence statusi: Complete.

P25632-1 [UniParc]FASTAAdd to Basket

« Hide

MVTQTNPVPV TYPTDAYIPT YLPDDKVSNL ADLKKLIEMD SRLDLYLTRR    50
RLDTSINLPT NTKTKDHPPN KEMLRIYVYN TTESSPRSDS GTPADSGKTT 100
WTLRIEGKLL HESANGKHPF SEFLEGVAVD FKRLKPLGMG KKRKRDSSLS 150
LPLNLQQPEY NDQDSTMGDN DNGEDEDSAE AESREEIVDA LEWNYDENNV 200
VEFDGIDIKR QGKDNLRCSI TIQLRGVDGG KVQYSPNLAT LIGMQTGSVN 250
DAVYSIYKYI LINNLFVTEQ TEAQDGSNDA EDSSNENNNK NGAGDDDGVE 300
GSTPKDKPEL GEVKLDSLLQ KVLDTNAAHL PLMNVVQTVN KLVSPLPPII 350
LDYTIDLSKD TTYGATTLDV DVSHILHQPQ PQPNLQKEEE TDAEDTAKLR 400
EITKLALQLN SSAQKYQFFH ELSLHPRETL THYLWSSKQN ELVLQGDQYF 450
NEDAARTSDI YSNNNNDRSL MGNISLLYSQ GRL 483
Length:483
Mass (Da):54,165
Last modified:May 1, 1992 - v1
Checksum:i6A9998BDBC4D24B2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42283.1.
AY692985 Genomic DNA. Translation: AAT93004.1.
BK006937 Genomic DNA. Translation: DAA07529.1.
PIRiS19466.
RefSeqiNP_009981.1. NM_001178766.1.

Genome annotation databases

EnsemblFungiiYCR052W; YCR052W; YCR052W.
GeneIDi850419.
KEGGisce:YCR052W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAA42283.1 .
AY692985 Genomic DNA. Translation: AAT93004.1 .
BK006937 Genomic DNA. Translation: DAA07529.1 .
PIRi S19466.
RefSeqi NP_009981.1. NM_001178766.1.

3D structure databases

ProteinModelPortali P25632.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31032. 123 interactions.
DIPi DIP-4999N.
IntActi P25632. 48 interactions.
MINTi MINT-493435.
STRINGi 4932.YCR052W.

Proteomic databases

MaxQBi P25632.
PaxDbi P25632.
PeptideAtlasi P25632.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR052W ; YCR052W ; YCR052W .
GeneIDi 850419.
KEGGi sce:YCR052W.

Organism-specific databases

CYGDi YCR052w.
SGDi S000000648. RSC6.

Phylogenomic databases

eggNOGi COG5531.
GeneTreei ENSGT00730000114141.
HOGENOMi HOG000000860.
KOi K11760.
OMAi LHESANG.
OrthoDBi EOG7X0VS9.

Enzyme and pathway databases

BioCyci YEAST:G3O-29361-MONOMER.

Miscellaneous databases

NextBioi 965983.

Gene expression databases

Genevestigatori P25632.

Family and domain databases

Gene3Di 1.10.245.10. 2 hits.
InterProi IPR003121. SWIB_MDM2_domain.
[Graphical view ]
SUPFAMi SSF47592. SSF47592. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: PROTEIN SEQUENCE OF 416-427, FUNCTION, COMPOSITION OF THE RSC COMPLEX.
  5. "Direct interaction between Rsc6 and Rsc8/Swh3, two proteins that are conserved in SWI/SNF-related complexes."
    Treich I., Ho L., Carlson M.
    Nucleic Acids Res. 26:3739-3745(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RSC8.
  6. "Histone octamer transfer by a chromatin-remodeling complex."
    Lorch Y., Zhang M., Kornberg R.D.
    Cell 96:389-392(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  7. "Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC."
    Moreira J.M.A., Holmberg S.
    EMBO J. 18:2836-2844(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  8. "Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains."
    Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F.
    Mol. Cell 4:715-723(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE RSC COMPLEX.
  9. "Chromatin remodeling by RSC involves ATP-dependent DNA translocation."
    Saha A., Wittmeyer J., Cairns B.R.
    Genes Dev. 16:2120-2134(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  10. "Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway."
    Chai B., Hsu J.-M., Du J., Laurent B.C.
    Genetics 161:575-584(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  11. "The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation."
    Hsu J.-M., Huang J., Meluh P.B., Laurent B.C.
    Mol. Cell. Biol. 23:3202-3215(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRSC6_YEAST
AccessioniPrimary (citable) accession number: P25632
Secondary accession number(s): D6VR60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 11, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2470 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

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