Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P25632

- RSC6_YEAST

UniProt

P25632 - RSC6_YEAST

Protein

Chromatin structure-remodeling complex protein RSC6

Gene

RSC6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and suppresses formamide sensitivity of the RSC8 mutants.7 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. ATP-dependent chromatin remodeling Source: UniProtKB
    3. G2/M transition of mitotic cell cycle Source: SGD
    4. nucleosome disassembly Source: SGD
    5. nucleosome positioning Source: SGD
    6. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29361-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chromatin structure-remodeling complex protein RSC6
    Alternative name(s):
    Remodel the structure of chromatin complex subunit 6
    Gene namesi
    Name:RSC6
    Ordered Locus Names:YCR052W
    ORF Names:YCR52W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR052w.
    SGDiS000000648. RSC6.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.

    GO - Cellular componenti

    1. RSC complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 483483Chromatin structure-remodeling complex protein RSC6PRO_0000097474Add
    BLAST

    Proteomic databases

    MaxQBiP25632.
    PaxDbiP25632.
    PeptideAtlasiP25632.

    Expressioni

    Gene expression databases

    GenevestigatoriP25632.

    Interactioni

    Subunit structurei

    Interacts directly with RSC8. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RSC58Q079797EBI-21941,EBI-36549
    RSC8P4360912EBI-21941,EBI-23005

    Protein-protein interaction databases

    BioGridi31032. 123 interactions.
    DIPiDIP-4999N.
    IntActiP25632. 48 interactions.
    MINTiMINT-493435.
    STRINGi4932.YCR052W.

    Structurei

    3D structure databases

    ProteinModelPortaliP25632.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    To yeast SNF12.Curated

    Phylogenomic databases

    eggNOGiCOG5531.
    GeneTreeiENSGT00730000114141.
    HOGENOMiHOG000000860.
    KOiK11760.
    OMAiLHESANG.
    OrthoDBiEOG7X0VS9.

    Family and domain databases

    Gene3Di1.10.245.10. 2 hits.
    InterProiIPR003121. SWIB_MDM2_domain.
    [Graphical view]
    SUPFAMiSSF47592. SSF47592. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P25632-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTQTNPVPV TYPTDAYIPT YLPDDKVSNL ADLKKLIEMD SRLDLYLTRR    50
    RLDTSINLPT NTKTKDHPPN KEMLRIYVYN TTESSPRSDS GTPADSGKTT 100
    WTLRIEGKLL HESANGKHPF SEFLEGVAVD FKRLKPLGMG KKRKRDSSLS 150
    LPLNLQQPEY NDQDSTMGDN DNGEDEDSAE AESREEIVDA LEWNYDENNV 200
    VEFDGIDIKR QGKDNLRCSI TIQLRGVDGG KVQYSPNLAT LIGMQTGSVN 250
    DAVYSIYKYI LINNLFVTEQ TEAQDGSNDA EDSSNENNNK NGAGDDDGVE 300
    GSTPKDKPEL GEVKLDSLLQ KVLDTNAAHL PLMNVVQTVN KLVSPLPPII 350
    LDYTIDLSKD TTYGATTLDV DVSHILHQPQ PQPNLQKEEE TDAEDTAKLR 400
    EITKLALQLN SSAQKYQFFH ELSLHPRETL THYLWSSKQN ELVLQGDQYF 450
    NEDAARTSDI YSNNNNDRSL MGNISLLYSQ GRL 483
    Length:483
    Mass (Da):54,165
    Last modified:May 1, 1992 - v1
    Checksum:i6A9998BDBC4D24B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42283.1.
    AY692985 Genomic DNA. Translation: AAT93004.1.
    BK006937 Genomic DNA. Translation: DAA07529.1.
    PIRiS19466.
    RefSeqiNP_009981.1. NM_001178766.1.

    Genome annotation databases

    EnsemblFungiiYCR052W; YCR052W; YCR052W.
    GeneIDi850419.
    KEGGisce:YCR052W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42283.1 .
    AY692985 Genomic DNA. Translation: AAT93004.1 .
    BK006937 Genomic DNA. Translation: DAA07529.1 .
    PIRi S19466.
    RefSeqi NP_009981.1. NM_001178766.1.

    3D structure databases

    ProteinModelPortali P25632.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31032. 123 interactions.
    DIPi DIP-4999N.
    IntActi P25632. 48 interactions.
    MINTi MINT-493435.
    STRINGi 4932.YCR052W.

    Proteomic databases

    MaxQBi P25632.
    PaxDbi P25632.
    PeptideAtlasi P25632.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR052W ; YCR052W ; YCR052W .
    GeneIDi 850419.
    KEGGi sce:YCR052W.

    Organism-specific databases

    CYGDi YCR052w.
    SGDi S000000648. RSC6.

    Phylogenomic databases

    eggNOGi COG5531.
    GeneTreei ENSGT00730000114141.
    HOGENOMi HOG000000860.
    KOi K11760.
    OMAi LHESANG.
    OrthoDBi EOG7X0VS9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29361-MONOMER.

    Miscellaneous databases

    NextBioi 965983.

    Gene expression databases

    Genevestigatori P25632.

    Family and domain databases

    Gene3Di 1.10.245.10. 2 hits.
    InterProi IPR003121. SWIB_MDM2_domain.
    [Graphical view ]
    SUPFAMi SSF47592. SSF47592. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: PROTEIN SEQUENCE OF 416-427, FUNCTION, COMPOSITION OF THE RSC COMPLEX.
    5. "Direct interaction between Rsc6 and Rsc8/Swh3, two proteins that are conserved in SWI/SNF-related complexes."
      Treich I., Ho L., Carlson M.
      Nucleic Acids Res. 26:3739-3745(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RSC8.
    6. "Histone octamer transfer by a chromatin-remodeling complex."
      Lorch Y., Zhang M., Kornberg R.D.
      Cell 96:389-392(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSC COMPLEX.
    7. "Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC."
      Moreira J.M.A., Holmberg S.
      EMBO J. 18:2836-2844(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSC COMPLEX.
    8. "Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains."
      Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F.
      Mol. Cell 4:715-723(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: COMPOSITION OF THE RSC COMPLEX.
    9. "Chromatin remodeling by RSC involves ATP-dependent DNA translocation."
      Saha A., Wittmeyer J., Cairns B.R.
      Genes Dev. 16:2120-2134(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSC COMPLEX.
    10. "Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway."
      Chai B., Hsu J.-M., Du J., Laurent B.C.
      Genetics 161:575-584(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSC COMPLEX.
    11. "The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation."
      Hsu J.-M., Huang J., Meluh P.B., Laurent B.C.
      Mol. Cell. Biol. 23:3202-3215(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRSC6_YEAST
    AccessioniPrimary (citable) accession number: P25632
    Secondary accession number(s): D6VR60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2470 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3