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Protein

Sterol O-acyltransferase 1

Gene

ARE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acyl-CoA + cholesterol = CoA + cholesterol ester.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei547 – 5471Sequence analysis

GO - Molecular functioni

  • ergosterol O-acyltransferase activity Source: SGD
  • lanosterol O-acyltransferase activity Source: SGD

GO - Biological processi

  • ergosterol metabolic process Source: GO_Central
  • sterol metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YCR048W-MONOMER.
YEAST:YCR048W-MONOMER.
ReactomeiR-SCE-1482883. Acyl chain remodeling of DAG and TAG.
R-SCE-75109. Triglyceride Biosynthesis.

Chemistry

SwissLipidsiSLP:000000706.
SLP:000000709.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol O-acyltransferase 1 (EC:2.3.1.26)
Alternative name(s):
Sterol-ester synthase 1
Gene namesi
Name:ARE1
Synonyms:SAT2
Ordered Locus Names:YCR048W
ORF Names:YCR48W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR048W.
SGDiS000000644. ARE1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei182 – 20221HelicalSequence analysisAdd
BLAST
Transmembranei229 – 24921HelicalSequence analysisAdd
BLAST
Transmembranei264 – 28421HelicalSequence analysisAdd
BLAST
Transmembranei371 – 39121HelicalSequence analysisAdd
BLAST
Transmembranei409 – 42921HelicalSequence analysisAdd
BLAST
Transmembranei535 – 55521HelicalSequence analysisAdd
BLAST
Transmembranei590 – 61021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610Sterol O-acyltransferase 1PRO_0000207648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211PhosphoserineCombined sources
Modified residuei45 – 451PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP25628.
TopDownProteomicsiP25628.

PTM databases

iPTMnetiP25628.

Interactioni

Protein-protein interaction databases

BioGridi31030. 64 interactions.
DIPiDIP-4998N.
IntActiP25628. 4 interactions.
MINTiMINT-552394.

Structurei

3D structure databases

ProteinModelPortaliP25628.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063122.
HOGENOMiHOG000210250.
InParanoidiP25628.
KOiK00637.
OMAiQINYPRT.
OrthoDBiEOG092C2MBI.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
[Graphical view]
PANTHERiPTHR10408. PTHR10408. 1 hit.
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.

Sequencei

Sequence statusi: Complete.

P25628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETKDLLQD EEFLKIRRLN SAEANKRHSV TYDNVILPQE SMEVSPRSST
60 70 80 90 100
TSLVEPVEST EGVESTEAER VAGKQEQEEE YPVDAHMQKY LSHLKSKSRS
110 120 130 140 150
RFHRKDASKY VSFFGDVSFD PRPTLLDSAI NVPFQTTFKG PVLEKQLKNL
160 170 180 190 200
QLTKTKTKAT VKTTVKTTEK TDKADAPPGE KLESNFSGIY VFAWMFLGWI
210 220 230 240 250
AIRCCTDYYA SYGSAWNKLE IVQYMTTDLF TIAMLDLAMF LCTFFVVFVH
260 270 280 290 300
WLVKKRIINW KWTGFVAVSI FELAFIPVTF PIYVYYFDFN WVTRIFLFLH
310 320 330 340 350
SVVFVMKSHS FAFYNGYLWD IKQELEYSSK QLQKYKESLS PETREILQKS
360 370 380 390 400
CDFCLFELNY QTKDNDFPNN ISCSNFFMFC LFPVLVYQIN YPRTSRIRWR
410 420 430 440 450
YVLEKVCAII GTIFLMMVTA QFFMHPVAMR CIQFHNTPTF GGWIPATQEW
460 470 480 490 500
FHLLFDMIPG FTVLYMLTFY MIWDALLNCV AELTRFADRY FYGDWWNCVS
510 520 530 540 550
FEEFSRIWNV PVHKFLLRHV YHSSMGALHL SKSQATLFTF FLSAVFHEMA
560 570 580 590 600
MFAIFRRVRG YLFMFQLSQF VWTALSNTKF LRARPQLSNV VFSFGVCSGP
610
SIIMTLYLTL
Length:610
Mass (Da):71,613
Last modified:May 1, 1992 - v1
Checksum:iFF72EFB9238B2205
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42296.1.
AY692921 Genomic DNA. Translation: AAT92940.1.
BK006937 Genomic DNA. Translation: DAA07526.1.
PIRiS19461.
RefSeqiNP_009978.1. NM_001178762.1.

Genome annotation databases

EnsemblFungiiCAA42296; CAA42296; CAA42296.
YCR048W; YCR048W; YCR048W.
GeneIDi850415.
KEGGisce:YCR048W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42296.1.
AY692921 Genomic DNA. Translation: AAT92940.1.
BK006937 Genomic DNA. Translation: DAA07526.1.
PIRiS19461.
RefSeqiNP_009978.1. NM_001178762.1.

3D structure databases

ProteinModelPortaliP25628.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31030. 64 interactions.
DIPiDIP-4998N.
IntActiP25628. 4 interactions.
MINTiMINT-552394.

Chemistry

SwissLipidsiSLP:000000706.
SLP:000000709.

PTM databases

iPTMnetiP25628.

Proteomic databases

MaxQBiP25628.
TopDownProteomicsiP25628.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42296; CAA42296; CAA42296.
YCR048W; YCR048W; YCR048W.
GeneIDi850415.
KEGGisce:YCR048W.

Organism-specific databases

EuPathDBiFungiDB:YCR048W.
SGDiS000000644. ARE1.

Phylogenomic databases

GeneTreeiENSGT00530000063122.
HOGENOMiHOG000210250.
InParanoidiP25628.
KOiK00637.
OMAiQINYPRT.
OrthoDBiEOG092C2MBI.

Enzyme and pathway databases

BioCyciMetaCyc:YCR048W-MONOMER.
YEAST:YCR048W-MONOMER.
ReactomeiR-SCE-1482883. Acyl chain remodeling of DAG and TAG.
R-SCE-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiP25628.

Family and domain databases

InterProiIPR004299. MBOAT_fam.
IPR014371. Oat_ACAT_DAG_ARE.
[Graphical view]
PANTHERiPTHR10408. PTHR10408. 1 hit.
PfamiPF03062. MBOAT. 1 hit.
[Graphical view]
PIRSFiPIRSF000439. Oat_ACAT_DAG_ARE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARE1_YEAST
AccessioniPrimary (citable) accession number: P25628
Secondary accession number(s): D6VR57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 7, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.