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Reviewed, UniProtKB/Swiss-Prot P25628 (ARE1_YEAST)

Last modified November 3, 2009. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Sterol O-acyltransferase 1
    EC=2.3.1.26
Alternative name(s):
    Sterol-ester synthase 1
Gene names
Name: ARE1
Synonyms: SAT2
Ordered Locus Names: YCR048W
ORF Names: YCR48W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length610 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Acyl-CoA + cholesterol = CoA + cholesterol ester.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 610610Sterol O-acyltransferase 1
PRO_0000207648

Regions

Transmembrane182 – 20221 Potential
Transmembrane229 – 24921 Potential
Transmembrane264 – 28421 Potential
Transmembrane371 – 39121 Potential
Transmembrane409 – 42921 Potential
Transmembrane535 – 55521 Potential
Transmembrane590 – 61021 Potential

Sites

Active site5471 Potential

Amino acid modifications

Modified residue211Phosphoserine Ref.5 Ref.6
Modified residue451Phosphoserine Ref.6 Ref.4 Ref.7
Modified residue481Phosphoserine Ref.7

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Sequences

Sequence LengthMass (Da)Tools
P25628-1 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: FF72EFB9238B2205

FASTA61071,613
        10         20         30         40         50         60 
MTETKDLLQD EEFLKIRRLN SAEANKRHSV TYDNVILPQE SMEVSPRSST TSLVEPVEST 

        70         80         90        100        110        120 
EGVESTEAER VAGKQEQEEE YPVDAHMQKY LSHLKSKSRS RFHRKDASKY VSFFGDVSFD 

       130        140        150        160        170        180 
PRPTLLDSAI NVPFQTTFKG PVLEKQLKNL QLTKTKTKAT VKTTVKTTEK TDKADAPPGE 

       190        200        210        220        230        240 
KLESNFSGIY VFAWMFLGWI AIRCCTDYYA SYGSAWNKLE IVQYMTTDLF TIAMLDLAMF 

       250        260        270        280        290        300 
LCTFFVVFVH WLVKKRIINW KWTGFVAVSI FELAFIPVTF PIYVYYFDFN WVTRIFLFLH 

       310        320        330        340        350        360 
SVVFVMKSHS FAFYNGYLWD IKQELEYSSK QLQKYKESLS PETREILQKS CDFCLFELNY 

       370        380        390        400        410        420 
QTKDNDFPNN ISCSNFFMFC LFPVLVYQIN YPRTSRIRWR YVLEKVCAII GTIFLMMVTA 

       430        440        450        460        470        480 
QFFMHPVAMR CIQFHNTPTF GGWIPATQEW FHLLFDMIPG FTVLYMLTFY MIWDALLNCV 

       490        500        510        520        530        540 
AELTRFADRY FYGDWWNCVS FEEFSRIWNV PVHKFLLRHV YHSSMGALHL SKSQATLFTF 

       550        560        570        580        590        600 
FLSAVFHEMA MFAIFRRVRG YLFMFQLSQF VWTALSNTKF LRARPQLSNV VFSFGVCSGP 

       610 
SIIMTLYLTL

« Hide

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References

« Hide 'large scale' references
[1]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed: 1574125] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Sterol esterification in yeast: a two-gene process."
Yang H., Bard M., Bruner D.A., Gleeson A., Deckelbaum R.J., Aljinovic G., Pohl T.M., Rothstein R., Sturley S.L.
Science 272:1353-1356(1996) [PubMed: 8650549] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45, MASS SPECTROMETRY.
[5]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, MASS SPECTROMETRY.
[6]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21 AND SER-45, MASS SPECTROMETRY.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND SER-48, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X59720 Genomic DNA. Translation: CAA42296.1.
AY692921 Genomic DNA. Translation: AAT92940.1.
PIRS19461.
RefSeqNP_009978.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:4998N.
IntActP25628. 4 interactions.
STRINGP25628.

Genome annotation databases

EnsemblYCR048W; YCR048W; YCR048W; Saccharomyces cerevisiae. [Genome view]
GeneID850415.
GenomeReviewsGene locus YCR048W in contig X59720_GR.
KEGGsce:YCR048W.
NMPDRfig|4932.3.peg.706.

Organism-specific databases

CYGDYCR048w.
SGDS000000644. ARE1.

Phylogenomic databases

HOGENOMP25628.
OMAQINYPRT.

Enzyme and pathway databases

BRENDA2.3.1.26. 250.

Gene expression databases

ArrayExpressP25628.
GenevestigatorP25628.
GermOnlineYCR048W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004299. MBOAT_fam.
[Graphical view]
PfamPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio965974.

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Entry information

Entry nameARE1_YEAST
AccessionPrimary (citable) accession number: P25628
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 3, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents