P25627 (BUD23_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative methyltransferase BUD23
EC=2.1.1.-

Alternative name(s):
Bud site selection protein 23

Gene names

Name:	BUD23
Ordered Locus Names:	YCR047C
ORF Names:	YCR47C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative methyltransferase involved in positioning the proximal bud pole signal. Ref.6
Subcellular location	Cytoplasm. Nucleus Ref.8.
Miscellaneous	Present with 1620 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the methyltransferase superfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular bud site selection Inferred from mutant phenotype Ref.6. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 18332120. Source: SGD rRNA (guanine-N7)-methylation Inferred from mutant phenotype PubMed 18332120. Source: SGD ribosomal small subunit export from nucleus Inferred from mutant phenotype PubMed 18332120. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.8. Source: SGD nucleolus Inferred from direct assay PubMed 18332120. Source: SGD
Molecular_function	rRNA (guanine) methyltransferase activity Inferred from mutant phenotype PubMed 18332120. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Putative methyltransferase BUD23		PRO_0000204459

Sequences

	Sequence		Length	Mass (Da)
	P25627 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: A8011A097C461900	FASTA	275	30,742
	10 20 30 40 50 60 MSRPEELAPP EIFYNDSEAH KYTGSTRVQH IQAKMTLRAL ELLNLQPCSF ILDIGCGSGL 70 80 90 100 110 120 SGEILTQEGD HVWCGLDISP SMLATGLSRE LEGDLMLQDM GTGIPFRAGS FDAAISISAI 130 140 150 160 170 180 QWLCNADTSY NDPKQRLMRF FNTLYAALKK GGKFVAQFYP KNDDQVDDIL QSAKVAGFSG 190 200 210 220 230 240 GLVVDDPESK KNKKYYLVLS SGAPPQGEEQ VNLDGVTMDE ENVNLKKQLR QRLKGGKDKE 250 260 270 SAKSFILRKK ELMKRRGRKV AKDSKFTGRK RRHRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Comprehensive sequence analysis of the 182 predicted open reading frames of yeast chromosome III." Bork P., Ouzounis C., Sander C., Scharf M., Schneider R., Sonnhammer E. Protein Sci. 1:1677-1690(1992) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY TO METHYLTRANSFERASES.
[5]	"S-adenosylmethionine-dependent methylation in Saccharomyces cerevisiae. Identification of a novel protein arginine methyltransferase." Niewmierzycka A., Clarke S. J. Biol. Chem. 274:814-824(1999) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY TO METHYLTRANSFERASES.
[6]	"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae." Ni L., Snyder M. Mol. Biol. Cell 12:2147-2170(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Automated identification of putative methyltransferases from genomic open reading frames." Katz J.E., Dlakic M., Clarke S. Mol. Cell. Proteomics 2:525-540(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY TO METHYLTRANSFERASES.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59720 Genomic DNA. Translation: CAA42295.1. AY692877 Genomic DNA. Translation: AAT92896.1. BK006937 Genomic DNA. Translation: DAA07525.1.
PIR	S19460.
RefSeq	NP_009976.1. NM_001178761.1.
3D structure databases
ProteinModelPortal	P25627.
SMR	P25627. Positions 40-163.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4673N.
IntAct	P25627. 1 interaction.
MINT	MINT-515776.
STRING	4932.YCR047C.
Proteomic databases
PaxDb	P25627.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCR047C; YCR047C; YCR047C.
GeneID	850414.
KEGG	sce:YCR047C.
Organism-specific databases
CYGD	YCR047c.
SGD	S000000643. BUD23.
Phylogenomic databases
eggNOG	COG0500.
GeneTree	ENSGT00390000014737.
HOGENOM	HOG000111527.
OMA	PFKPGSF.
OrthoDB	EOG44XNRR.
Gene expression databases
Genevestigator	P25627.
GermOnline	YCR047C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013216. Methyltransf_11. IPR022238. Unchr_MeTrfase_Williams-Beuren. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF12589. WBS_methylT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	965971.

Entry information

Entry name

BUD23_YEAST

Accession

Primary (citable) accession number: P25627
Secondary accession number(s): D6VR56

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program