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Protein

18S rRNA (guanine(1575)-N(7))-methyltransferase

Gene

BUD23

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N7 position of guanine 1575 (m7G1575) in 18S rRNA. Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity. Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity. Required for efficient cleavage of the primary 35S precursor rRNA at site A2. Involved in positioning the proximal bud pole signal.6 Publications

Catalytic activityi

S-adenosyl-L-methionine + guanine(1575) in 18S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(1575) in 18S rRNA.1 Publication

GO - Molecular functioni

  • rRNA (guanine) methyltransferase activity Source: SGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: SGD

GO - Biological processi

  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal small subunit export from nucleus Source: SGD
  • rRNA (guanine-N7)-methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29358-MONOMER.
YEAST:G3O-29358-MONOMER.
BRENDAi2.1.1.B65. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
18S rRNA (guanine(1575)-N(7))-methyltransferase (EC:2.1.1.3091 Publication)
Alternative name(s):
Bud site selection protein 23
Gene namesi
Name:BUD23
Ordered Locus Names:YCR047C
ORF Names:YCR47C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR047C.
SGDiS000000643. BUD23.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57G → E: Fails to ctalyze the N-7 methylation of the G1575 residue. 2 Publications1
Mutagenesisi57G → R: Can only partially restore the slow-growth phenotype and the random budding pattern of a null mutant. 2 Publications1
Mutagenesisi77D → A: No effect on growth and budding pattern. 2 Publications1
Mutagenesisi77D → K: Fails to ctalyze the N-7 methylation of the G1575 residue. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002044591 – 27518S rRNA (guanine(1575)-N(7))-methyltransferaseAdd BLAST275

Proteomic databases

MaxQBiP25627.
PRIDEiP25627.

Interactioni

Subunit structurei

Interacts with TRM112. Interacts with ECM16.3 Publications

Protein-protein interaction databases

BioGridi31029. 43 interactors.
DIPiDIP-4673N.
IntActiP25627. 1 interactor.
MINTiMINT-515776.

Structurei

Secondary structure

1275
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 24Combined sources9
Helixi25 – 43Combined sources19
Beta strandi50 – 54Combined sources5
Helixi60 – 69Combined sources10
Beta strandi72 – 78Combined sources7
Helixi80 – 87Combined sources8
Turni88 – 90Combined sources3
Beta strandi92 – 97Combined sources6
Helixi100 – 102Combined sources3
Beta strandi111 – 118Combined sources8
Helixi120 – 124Combined sources5
Helixi133 – 147Combined sources15
Beta strandi148 – 158Combined sources11
Helixi163 – 176Combined sources14
Beta strandi179 – 186Combined sources8
Turni190 – 192Combined sources3
Beta strandi194 – 200Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QTTX-ray2.00B/D1-202[»]
4QTUX-ray2.12B/D1-202[»]
ProteinModelPortaliP25627.
SMRiP25627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi257 – 264Nuclear localization signalSequence analysis8

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000014737.
HOGENOMiHOG000111527.
InParanoidiP25627.
KOiK19306.
OMAiNDAQRSM.
OrthoDBiEOG092C4WO9.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
IPR022238. Unchr_MeTrfase_Williams-Beuren.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF12589. WBS_methylT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P25627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPEELAPP EIFYNDSEAH KYTGSTRVQH IQAKMTLRAL ELLNLQPCSF
60 70 80 90 100
ILDIGCGSGL SGEILTQEGD HVWCGLDISP SMLATGLSRE LEGDLMLQDM
110 120 130 140 150
GTGIPFRAGS FDAAISISAI QWLCNADTSY NDPKQRLMRF FNTLYAALKK
160 170 180 190 200
GGKFVAQFYP KNDDQVDDIL QSAKVAGFSG GLVVDDPESK KNKKYYLVLS
210 220 230 240 250
SGAPPQGEEQ VNLDGVTMDE ENVNLKKQLR QRLKGGKDKE SAKSFILRKK
260 270
ELMKRRGRKV AKDSKFTGRK RRHRF
Length:275
Mass (Da):30,742
Last modified:May 1, 1992 - v1
Checksum:iA8011A097C461900
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42295.1.
AY692877 Genomic DNA. Translation: AAT92896.1.
BK006937 Genomic DNA. Translation: DAA07525.1.
PIRiS19460.
RefSeqiNP_009976.1. NM_001178761.1.

Genome annotation databases

EnsemblFungiiCAA42295; CAA42295; CAA42295.
YCR047C; YCR047C; YCR047C.
GeneIDi850414.
KEGGisce:YCR047C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAA42295.1.
AY692877 Genomic DNA. Translation: AAT92896.1.
BK006937 Genomic DNA. Translation: DAA07525.1.
PIRiS19460.
RefSeqiNP_009976.1. NM_001178761.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QTTX-ray2.00B/D1-202[»]
4QTUX-ray2.12B/D1-202[»]
ProteinModelPortaliP25627.
SMRiP25627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31029. 43 interactors.
DIPiDIP-4673N.
IntActiP25627. 1 interactor.
MINTiMINT-515776.

Proteomic databases

MaxQBiP25627.
PRIDEiP25627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAA42295; CAA42295; CAA42295.
YCR047C; YCR047C; YCR047C.
GeneIDi850414.
KEGGisce:YCR047C.

Organism-specific databases

EuPathDBiFungiDB:YCR047C.
SGDiS000000643. BUD23.

Phylogenomic databases

GeneTreeiENSGT00390000014737.
HOGENOMiHOG000111527.
InParanoidiP25627.
KOiK19306.
OMAiNDAQRSM.
OrthoDBiEOG092C4WO9.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29358-MONOMER.
YEAST:G3O-29358-MONOMER.
BRENDAi2.1.1.B65. 984.

Miscellaneous databases

PROiP25627.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
IPR022238. Unchr_MeTrfase_Williams-Beuren.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF12589. WBS_methylT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBUD23_YEAST
AccessioniPrimary (citable) accession number: P25627
Secondary accession number(s): D6VR56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1620 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.