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Protein

Suppressor of yeast profilin deletion

Gene

SYP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multi-functional protein that contributes to the endocytic process, but also to events that occur at the neck during budding and/or cytokinesis. Plays a role as an endocytic adapters with membrane-tubulation activity that associates with transmembrane cargo proteins and initiates the formation of endocytic sites. Contributes to the stabilization of the nascent clathrin-coated pit. Plays also a role in late endocytosis by mediating vesiculation. Involved in the regulation of cell cycle-dependent dynamics of the septin cytoskeleton by promoting septin turnover in different cell cycle stages. May act through the RHO2 signaling pathway to repolarize cortical actin patches in profilin-deficient cells.4 Publications

GO - Molecular functioni

  • enzyme inhibitor activity Source: SGD

GO - Biological processi

  • actin cortical patch assembly Source: SGD
  • cell cycle Source: UniProtKB-KW
  • endocytosis Source: UniProtKB-KW
  • negative regulation of catalytic activity Source: SGD
  • septin cytoskeleton organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Endocytosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29344-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Suppressor of yeast profilin deletion
Gene namesi
Name:SYP1
Ordered Locus Names:YCR030C
ORF Names:YCR30C/YCR29C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR030C.
SGDiS000000626. SYP1.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: UniProtKB
  • cellular bud neck septin ring Source: SGD
  • cellular bud tip Source: UniProtKB
  • endocytic patch Source: SGD
  • mating projection base Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000723901 – 870Suppressor of yeast profilin deletionAdd BLAST870

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki256Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei264PhosphoserineCombined sources1
Modified residuei331PhosphoserineCombined sources1
Modified residuei416PhosphothreonineCombined sources1
Modified residuei496PhosphoserineCombined sources1
Modified residuei500PhosphoserineCombined sources1
Modified residuei577PhosphothreonineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP25623.
PRIDEiP25623.

PTM databases

iPTMnetiP25623.

Interactioni

Subunit structurei

Interacts with CDC3, CDC10, CDC11, CDC12, EDE1 and EPS15.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-21900,EBI-21900
EDE1P342167EBI-21900,EBI-21243
MID2P360272EBI-21900,EBI-10901
SLA1P327903EBI-21900,EBI-17313

Protein-protein interaction databases

BioGridi31013. 61 interactors.
DIPiDIP-1758N.
IntActiP25623. 32 interactors.
MINTiMINT-402640.

Structurei

Secondary structure

1870
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 11Combined sources5
Turni12 – 15Combined sources4
Helixi18 – 64Combined sources47
Helixi67 – 77Combined sources11
Helixi83 – 88Combined sources6
Helixi97 – 125Combined sources29
Helixi127 – 131Combined sources5
Beta strandi133 – 136Combined sources4
Helixi138 – 159Combined sources22
Helixi168 – 230Combined sources63
Helixi233 – 246Combined sources14
Beta strandi611 – 625Combined sources15
Beta strandi628 – 643Combined sources16
Beta strandi646 – 648Combined sources3
Beta strandi654 – 661Combined sources8
Helixi663 – 665Combined sources3
Beta strandi666 – 671Combined sources6
Turni673 – 675Combined sources3
Beta strandi676 – 680Combined sources5
Beta strandi683 – 686Combined sources4
Turni688 – 691Combined sources4
Beta strandi695 – 705Combined sources11
Beta strandi709 – 718Combined sources10
Beta strandi720 – 731Combined sources12
Beta strandi741 – 753Combined sources13
Beta strandi758 – 766Combined sources9
Beta strandi771 – 773Combined sources3
Beta strandi775 – 779Combined sources5
Beta strandi784 – 787Combined sources4
Beta strandi793 – 803Combined sources11
Beta strandi812 – 818Combined sources7
Turni827 – 830Combined sources4
Beta strandi836 – 845Combined sources10
Beta strandi856 – 869Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G9GX-ray2.40A1-264[»]
3G9HX-ray2.80A566-870[»]
ProteinModelPortaliP25623.
SMRiP25623.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25623.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini609 – 869MHDPROSITE-ProRule annotationAdd BLAST261

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi308 – 405Ser-richAdd BLAST98
Compositional biasi417 – 528Pro-richAdd BLAST112

Sequence similaritiesi

Belongs to the SYP1 family.Curated
Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000057127.
InParanoidiP25623.
KOiK20042.
OMAiQPIFEDE.
OrthoDBiEOG092C13HH.

Family and domain databases

InterProiIPR028565. MHD.
IPR018808. Muniscin_C.
[Graphical view]
PfamiPF10291. muHD. 1 hit.
[Graphical view]
PROSITEiPS51072. MHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEQRTKYAD SILTTKSPYE ATETIRIRLS QVKLLNKDFY LLFKELANLK
60 70 80 90 100
RNYAQQLRKI IAENEDITKI LNAQMIESNV LTPQEMSAFR FNSLGELRNV
110 120 130 140 150
WDTVIEELKS DLKSSTEYYN TLDQQVVREL KESVENNTSW RESKDLHSKL
160 170 180 190 200
SKNAASIEHY SKNNENSSHL EEARRQWDQQ SPYLFELFET IDYNRLDTLK
210 220 230 240 250
NCMLRFQTSF SDYLLNTTKE CETVMTKFLA FEPQSEIDRF AKDASQYNFQ
260 270 280 290 300
LSSSSKEVVP NNASPASATG ARPVSVSNGA ANTEREKKSP QKDKRKSAFG
310 320 330 340 350
NIGHRLASAS SSLTHNDLMN NEFSDSTNNS SLKSKKSSHT LRSKVGSIFG
360 370 380 390 400
RNKTKNKRQQ QSSSNSHIQA SITETPNNSS TRVSSTATSS IYQKQRRPTY
410 420 430 440 450
SSSKSNNWTP GEASDTPPLP PHATPKNVDA PVTADTPPAQ TFTPSEVPPS
460 470 480 490 500
TPQQSSPPTA KEPDSSNLPK TVPISISQPP LQPQSKTKPL PVEPASPSIS
510 520 530 540 550
LPTATVDNQP SGQVDSRPLH IRAPALPPSR KQNFIHNRDS QLYDSLPNHG
560 570 580 590 600
SGATPTSSSL SSIPQERPVS TLSSQITGEL RELNPQATGS STSLVGQSLF
610 620 630 640 650
QHSSLDTSQF GLNASIAEVL NASFKDGMLQ NSQLIGEIAL NYLPNSVMNS
660 670 680 690 700
PLPIGINLRI NNGAKFEKVI LNQAFIERVA PEEFKVNPSF IDSRTLGAIK
710 720 730 740 750
YSIKEPIAPI VIHPVWRFES HQASVVLTVK MSPSLPDEIS QIVIEDLVVF
760 770 780 790 800
VNIDGANATS ALSKPQGSFS KEKKRITWRF KEPVVLTRNG EGQRLIARFI
810 820 830 840 850
TDGLAHESAK GVITKFTISE TDNVALPHSG AGSGITLTCQ ELDENNPFGG
860 870
EWLDVNTKRT LTTGNYHGLA
Length:870
Mass (Da):96,137
Last modified:October 24, 2003 - v3
Checksum:i6F35C8F1562E41CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42980.1.
BK006937 Genomic DNA. Translation: DAA07509.1.
PIRiS74291.
RefSeqiNP_009959.2. NM_001178744.1.

Genome annotation databases

EnsemblFungiiCAC42980; CAC42980; CAC42980.
YCR030C; YCR030C; YCR030C.
GeneIDi850396.
KEGGisce:YCR030C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42980.1.
BK006937 Genomic DNA. Translation: DAA07509.1.
PIRiS74291.
RefSeqiNP_009959.2. NM_001178744.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G9GX-ray2.40A1-264[»]
3G9HX-ray2.80A566-870[»]
ProteinModelPortaliP25623.
SMRiP25623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31013. 61 interactors.
DIPiDIP-1758N.
IntActiP25623. 32 interactors.
MINTiMINT-402640.

PTM databases

iPTMnetiP25623.

Proteomic databases

MaxQBiP25623.
PRIDEiP25623.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAC42980; CAC42980; CAC42980.
YCR030C; YCR030C; YCR030C.
GeneIDi850396.
KEGGisce:YCR030C.

Organism-specific databases

EuPathDBiFungiDB:YCR030C.
SGDiS000000626. SYP1.

Phylogenomic databases

HOGENOMiHOG000057127.
InParanoidiP25623.
KOiK20042.
OMAiQPIFEDE.
OrthoDBiEOG092C13HH.

Enzyme and pathway databases

BioCyciYEAST:G3O-29344-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP25623.
PROiP25623.

Family and domain databases

InterProiIPR028565. MHD.
IPR018808. Muniscin_C.
[Graphical view]
PfamiPF10291. muHD. 1 hit.
[Graphical view]
PROSITEiPS51072. MHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYP1_YEAST
AccessioniPrimary (citable) accession number: P25623
Secondary accession number(s): D6VR40, P25622, Q96VH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 24, 2003
Last modified: November 2, 2016
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.