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P25621 (FEN2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pantothenate transporter FEN2
Alternative name(s):
Fenpropimorph resistance protein 2
Gene names
Name:FEN2
Ordered Locus Names:YCR028C
ORF Names:YCR28C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transports pantothenate into the cell. Also involved in the catabolite repression-mediated regulation of ergosterol biosynthesis and in fenpropimorph resistance. Ref.5 Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.6.

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the major facilitator superfamily. Allantoate permease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Pantothenate transporter FEN2
PRO_0000121367

Regions

Topological domain1 – 2727Cytoplasmic Potential
Transmembrane28 – 4821Helical; Potential
Topological domain49 – 7931Extracellular Potential
Transmembrane80 – 10021Helical; Potential
Topological domain101 – 1022Cytoplasmic Potential
Transmembrane103 – 12321Helical; Potential
Topological domain124 – 1329Extracellular Potential
Transmembrane133 – 15321Helical; Potential
Topological domain154 – 16411Cytoplasmic Potential
Transmembrane165 – 18521Helical; Potential
Topological domain186 – 19813Extracellular Potential
Transmembrane199 – 21921Helical; Potential
Topological domain220 – 27152Cytoplasmic Potential
Transmembrane272 – 29221Helical; Potential
Topological domain293 – 31220Extracellular Potential
Transmembrane313 – 33321Helical; Potential
Topological domain334 – 3429Cytoplasmic Potential
Transmembrane343 – 36321Helical; Potential
Topological domain364 – 3729Extracellular Potential
Transmembrane373 – 39321Helical; Potential
Topological domain394 – 4018Cytoplasmic Potential
Transmembrane402 – 42221Helical; Potential
Topological domain423 – 43412Extracellular Potential
Transmembrane435 – 45521Helical; Potential
Topological domain456 – 51257Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P25621 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 361942E74C62B3B4

FASTA51258,256
        10         20         30         40         50         60 
MMKESKSITQ HEVERESVSS KRAIKKRLLL FKIDLFVLSF VCLQYWINYV DRVGFTNAYI 

        70         80         90        100        110        120 
SGMKEDLKMV GNDLTVSNTV FMIGYIVGMV PNNLMLLCVP PRIWLSFCTF AWGLLTLGMY 

       130        140        150        160        170        180 
KVTSFKHICA IRFFQALFES CTFSGTHFVL GSWYKEDELP IRSAIFTGSG LVGSMFSGFM 

       190        200        210        220        230        240 
QTSIFTHLNG RNGLAGWRWL FIIDFCITLP IAIYGFIFFP GLPDQTSAVS KFSMTRYIFN 

       250        260        270        280        290        300 
EQELHYARRR LPARDESTRL DWSTIPRVLK RWHWWMFSLV WVLGGENLGF ASNSTFALWL 

       310        320        330        340        350        360 
QNQKYTLAQR NNYPSGIFAV GIVSTLCSAV YMSKIPRARH WHVSVFISLV MVIVAVLIRA 

       370        380        390        400        410        420 
DPLNPKVVFS AQYLGGVAYA GQAVFFSWAN IICHADLQER AIVLASMNMF SGAVNAWWSI 

       430        440        450        460        470        480 
LFFASDMVPK FERGCYALLA TAISSGIVSV VIRSLQIKEN LSKKQVPYID ANDMPGEDDD 

       490        500        510 
DDNQDNENDG DDESMEVELH NEEMAEISNP FR 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of 9.2 kb left of CRY1 on yeast chromosome III from strain AB972: evidence for a Ty insertion and functional analysis of open reading frame YCR28."
Carbone M.L.A., Panzeri L., Falconi M.M., Carcano C., Plevani P., Lucchini G.
Yeast 8:805-812(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Yeast chromosome III: new gene functions."
Koonin E.V., Bork P., Sander C.
EMBO J. 13:493-503(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO DAL5 FAMILY.
[5]"FEN2: a gene implicated in the catabolite repression-mediated regulation of ergosterol biosynthesis in yeast."
Marcireau C., Joets J., Pousset D., Guilloton M., Karst F.
Yeast 12:531-539(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The fenpropimorph resistance gene FEN2 from Saccharomyces cerevisiae encodes a plasma membrane H+-pantothenate symporter."
Stolz J., Sauer N.
J. Biol. Chem. 274:18747-18752(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59720 Genomic DNA. Translation: CAA42320.1.
BK006937 Genomic DNA. Translation: DAA07507.1.
PIRS19439.
RefSeqNP_009957.1. NM_001178743.1.

3D structure databases

ProteinModelPortalP25621.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31011. 39 interactions.
DIPDIP-7672N.
IntActP25621. 6 interactions.
MINTMINT-1357147.
STRING4932.YCR028C.

Protein family/group databases

TCDB2.A.1.14.18. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbP25621.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR028C; YCR028C; YCR028C.
GeneID850394.
KEGGsce:YCR028C.

Organism-specific databases

CYGDYCR028c.
SGDS000000623. FEN2.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00620000088258.
HOGENOMHOG000179304.
KOK03448.
OMAFASNSTF.
OrthoDBEOG7SV14V.

Enzyme and pathway databases

BioCycYEAST:G3O-29343-MONOMER.

Gene expression databases

GenevestigatorP25621.

Family and domain databases

InterProIPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
ProtoNetSearch...

Other

NextBio965917.

Entry information

Entry nameFEN2_YEAST
AccessionPrimary (citable) accession number: P25621
Secondary accession number(s): D6VR38
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families