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P25619

- HSP30_YEAST

UniProt

P25619 - HSP30_YEAST

Protein

30 kDa heat shock protein

Gene

HSP30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Probably cooperates with other heat shock proteins in the translocation of polypeptides through membranes. It may counteract the altering effect of heat shock on the plasma membrane.

    GO - Molecular functioni

    1. ion channel activity Source: InterPro

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: SGD
    2. cellular response to ethanol Source: SGD
    3. cellular response to heat Source: SGD
    4. cellular response to hydrogen peroxide Source: SGD
    5. cellular response to osmotic stress Source: SGD
    6. negative regulation of ATPase activity Source: SGD

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29337-MONOMER.

    Protein family/group databases

    TCDBi3.E.1.4.1. the ion-translocating microbial rhodopsin (mr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    30 kDa heat shock protein
    Gene namesi
    Name:HSP30
    Ordered Locus Names:YCR021C
    ORF Names:YCR21C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR021c.
    SGDiS000000615. HSP30.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 33233230 kDa heat shock proteinPRO_0000196284Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei308 – 3081Phosphoserine1 Publication
    Modified residuei331 – 3311Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP25619.
    PeptideAtlasiP25619.

    Expressioni

    Developmental stagei

    Expressed during the entry into stationary phase resulting from glucose limitation.

    Gene expression databases

    GenevestigatoriP25619.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-8563,EBI-8563
    AGP1P253761EBI-8563,EBI-2357
    CYS4P325821EBI-8563,EBI-4167
    FLC1Q089671EBI-8563,EBI-36673
    FMP27Q061791EBI-8563,EBI-32551
    FMP45Q076511EBI-8563,EBI-2051056
    GLN1P322881EBI-8563,EBI-7665
    GPA2P108231EBI-8563,EBI-7382
    HNM1P198071EBI-8563,EBI-8409
    HXT1P324651EBI-8563,EBI-8759
    HXT3P324661EBI-8563,EBI-8770
    HXT5P386951EBI-8563,EBI-8778
    HXT7P390041EBI-8563,EBI-8790
    IST2P382501EBI-8563,EBI-21520
    ITR1P306051EBI-8563,EBI-2050956
    MBF1O144671EBI-8563,EBI-2043025
    MID2P360271EBI-8563,EBI-10901
    NHA1Q992711EBI-8563,EBI-11808
    OSH7P387551EBI-8563,EBI-12641
    PDR12Q027851EBI-8563,EBI-13065
    PMP3P872841EBI-8563,EBI-13555
    PPZ1P265701EBI-8563,EBI-13807
    RNR2P099381EBI-8563,EBI-15240
    RTN1Q049471EBI-8563,EBI-38020
    SFK1P357351EBI-8563,EBI-26678
    SNA3P143591EBI-8563,EBI-26122
    SNQ2P325681EBI-8563,EBI-17590
    STE20Q034971EBI-8563,EBI-18285
    TCB3Q036401EBI-8563,EBI-27872
    TPO2P532831EBI-8563,EBI-2044753
    TPO4Q122561EBI-8563,EBI-37213
    UBP1P250371EBI-8563,EBI-19819
    YBL029C-AQ3E7561EBI-8563,EBI-2044812
    YGR130CP532781EBI-8563,EBI-23314
    YHR146WP388451EBI-8563,EBI-24770

    Protein-protein interaction databases

    BioGridi31004. 90 interactions.
    DIPiDIP-4586N.
    IntActiP25619. 31 interactions.
    MINTiMINT-511943.
    STRINGi4932.YCR021C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25619.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3434ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini56 – 6510CytoplasmicSequence Analysis
    Topological domaini87 – 12135ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini143 – 15715CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini179 – 1813ExtracellularSequence Analysis
    Topological domaini203 – 21513CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini237 – 24812ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini270 – 33263CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei35 – 5521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei66 – 8621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei122 – 14221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei158 – 17821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei182 – 20221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei216 – 23621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei249 – 26921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi290 – 33243Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5524.
    GeneTreeiENSGT00390000004481.
    HOGENOMiHOG000248243.
    OMAiAQEHIST.
    OrthoDBiEOG754J0K.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25619-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDTLSSFLN RNEALGLNPP HGLDMHITKR GSDWLWAVFA VFGFILLCYV    50
    VMFFIAENKG SRLTRYALAP AFLITFFEFF AFFTYASDLG WTGVQAEFNH 100
    VKVSKSITGE VPGIRQIFYS KYIAWFLSWP CLLFLIELAA STTGENDDIS 150
    ALDMVHSLLI QIVGTLFWVV SLLVGSLIKS TYKWGYYTIG AVAMLVTQGV 200
    ICQRQFFNLK TRGFNALMLC TCMVIVWLYF ICWGLSDGGN RIQPDGEAIF 250
    YGVLDLCVFA IYPCYLLIAV SRDGKLPRLS LTGGFSHHHA TDDVEDAAPE 300
    TKEAVPESPR ASGETAIHEP EPEAEQAVED TA 332
    Length:332
    Mass (Da):37,045
    Last modified:May 1, 1992 - v1
    Checksum:i260474A481D29AC5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93123 Unassigned DNA. Translation: AAA02903.1.
    X59720 Genomic DNA. Translation: CAA42313.1.
    BK006937 Genomic DNA. Translation: DAA07500.1.
    PIRiS31848.
    RefSeqiNP_009950.1. NM_001178735.1.

    Genome annotation databases

    EnsemblFungiiYCR021C; YCR021C; YCR021C.
    GeneIDi850385.
    KEGGisce:YCR021C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93123 Unassigned DNA. Translation: AAA02903.1 .
    X59720 Genomic DNA. Translation: CAA42313.1 .
    BK006937 Genomic DNA. Translation: DAA07500.1 .
    PIRi S31848.
    RefSeqi NP_009950.1. NM_001178735.1.

    3D structure databases

    ProteinModelPortali P25619.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31004. 90 interactions.
    DIPi DIP-4586N.
    IntActi P25619. 31 interactions.
    MINTi MINT-511943.
    STRINGi 4932.YCR021C.

    Protein family/group databases

    TCDBi 3.E.1.4.1. the ion-translocating microbial rhodopsin (mr) family.

    Proteomic databases

    PaxDbi P25619.
    PeptideAtlasi P25619.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR021C ; YCR021C ; YCR021C .
    GeneIDi 850385.
    KEGGi sce:YCR021C.

    Organism-specific databases

    CYGDi YCR021c.
    SGDi S000000615. HSP30.

    Phylogenomic databases

    eggNOGi COG5524.
    GeneTreei ENSGT00390000004481.
    HOGENOMi HOG000248243.
    OMAi AQEHIST.
    OrthoDBi EOG754J0K.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29337-MONOMER.

    Miscellaneous databases

    NextBioi 965897.

    Gene expression databases

    Genevestigatori P25619.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and sequence of HSP30, a yeast heat-shock gene coding for a hydrophobic membrane protein."
      Regnacq M., Boucherie H.
      Curr. Genet. 23:435-442(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 44827 / SKQ2N.
    2. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-331, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-308, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHSP30_YEAST
    AccessioniPrimary (citable) accession number: P25619
    Secondary accession number(s): D6VR31, Q04556
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7800 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3