SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P25618

- CWH43_YEAST

UniProt

P25618 - CWH43_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein CWH43

Gene
CWH43, YCR017C, YCR17C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the maintenance of cell wall integrity. Required for the replacement of the diacylglycerol moiety by ceramides during GPI-anchor maturation.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei802 – 8021 Inferred

GO - Biological processi

  1. cell wall organization Source: UniProtKB
  2. fungal-type cell wall organization Source: SGD
  3. GPI anchor biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-29332-MONOMER.

Protein family/group databases

TCDBi9.B.131.1.1. the post-gpi attachment protein (p-gap2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CWH43
Alternative name(s):
Calcofluor white hypersensitive protein
Gene namesi
Name:CWH43
Ordered Locus Names:YCR017C
ORF Names:YCR17C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR017c.
SGDiS000000610. CWH43.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein
Note: Concentrates to the bud tip of small budded cells and to the neck of dividing cells.2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77Cytoplasmic Reviewed prediction
Topological domaini29 – 6436Extracellular Reviewed predictionAdd
BLAST
Transmembranei65 – 8521Helical; Reviewed predictionAdd
BLAST
Topological domaini86 – 916Cytoplasmic Reviewed prediction
Transmembranei92 – 11221Helical; Reviewed predictionAdd
BLAST
Topological domaini113 – 1175Extracellular Reviewed prediction
Transmembranei118 – 13821Helical; Reviewed predictionAdd
BLAST
Topological domaini139 – 14911Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei150 – 17021Helical; Reviewed predictionAdd
BLAST
Topological domaini171 – 1755Extracellular Reviewed prediction
Transmembranei176 – 19621Helical; Reviewed predictionAdd
BLAST
Topological domaini197 – 27882Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei279 – 29921Helical; Reviewed predictionAdd
BLAST
Topological domaini300 – 3078Extracellular Reviewed prediction
Transmembranei308 – 32821Helical; Reviewed predictionAdd
BLAST
Topological domaini329 – 3302Cytoplasmic Reviewed prediction
Transmembranei331 – 35121Helical; Reviewed predictionAdd
BLAST
Topological domaini352 – 3521Extracellular Reviewed prediction
Transmembranei353 – 36816Helical; Reviewed predictionAdd
BLAST
Topological domaini369 – 38012Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei381 – 40121Helical; Reviewed predictionAdd
BLAST
Topological domaini402 – 42019Extracellular Reviewed predictionAdd
BLAST
Transmembranei421 – 44121Helical; Reviewed predictionAdd
BLAST
Topological domaini442 – 4509Cytoplasmic Reviewed prediction
Transmembranei451 – 47121Helical; Reviewed predictionAdd
BLAST
Topological domaini472 – 49524Extracellular Reviewed predictionAdd
BLAST
Transmembranei496 – 51621Helical; Reviewed predictionAdd
BLAST
Topological domaini517 – 5171Cytoplasmic Reviewed prediction
Transmembranei518 – 53821Helical; Reviewed predictionAdd
BLAST
Topological domaini539 – 5479Extracellular Reviewed prediction
Transmembranei548 – 56821Helical; Reviewed predictionAdd
BLAST
Topological domaini569 – 58517Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei586 – 60621Helical; Reviewed predictionAdd
BLAST
Topological domaini607 – 6137Extracellular Reviewed prediction
Transmembranei614 – 63421Helical; Reviewed predictionAdd
BLAST
Topological domaini635 – 6428Cytoplasmic Reviewed prediction
Transmembranei643 – 66321Helical; Reviewed predictionAdd
BLAST
Topological domaini664 – 953290Extracellular Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cellular bud neck Source: UniProtKB
  2. cellular bud tip Source: UniProtKB
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi57 – 571G → R: Causes destabilization of the protein and induces the release of cell wall proteins in the culture medium. 2 Publications
Mutagenesisi472 – 4721H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi693 – 6931D → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi713 – 7131D → A: Impairs the introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi770 – 7701H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi771 – 7711H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi802 – 8021H → A: Abrogates the introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi807 – 8071E → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi862 – 8621D → A: Impairs the introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi882 – 8821R → A: Abrogates the introduction of ceramides into the GPI anchor. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Protein CWH43PRO_0000021052Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi419 – 4191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi490 – 4901N-linked (GlcNAc...) Reviewed prediction
Glycosylationi767 – 7671N-linked (GlcNAc...) Reviewed prediction
Glycosylationi792 – 7921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi825 – 8251N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP25618.
PaxDbiP25618.
PeptideAtlasiP25618.

Expressioni

Gene expression databases

GenevestigatoriP25618.

Interactioni

Protein-protein interaction databases

BioGridi30996. 83 interactions.
MINTiMINT-4478332.
STRINGi4932.YCR017C.

Structurei

3D structure databases

ProteinModelPortaliP25618.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 229229PGAP2-likeAdd
BLAST
Regioni230 – 953724PGAP2IP-likeAdd
BLAST
Regioni862 – 88221Required for function in lipid remodelingAdd
BLAST

Domaini

The PGAP2-like region interacts with the PGAP2IP-like region.1 Publication

Sequence similaritiesi

In the N-terminal section; belongs to the PGAP2 family.
In the C-terminal section; belongs to the PGAP2IP family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG309644.
GeneTreeiENSGT00510000048509.
HOGENOMiHOG000196377.
OMAiGNRDTTQ.
OrthoDBiEOG77DJF6.

Family and domain databases

InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR019402. Frag1/DRAM/Sfk1.
IPR027317. PGAP2IP.
[Graphical view]
PANTHERiPTHR14859:SF0. PTHR14859:SF0. 1 hit.
PfamiPF10277. Frag1. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequencei

Sequence statusi: Complete.

P25618-1 [UniParc]FASTAAdd to Basket

« Hide

MLIINGKIIP IAHTICAFSA FFAALVTGYS LHFHKIVTNA HYTYPDEWFP    50
SVSATIGDRY PERSIFQILI ALTAFPRFLL LLGHYYLNQS KVCFLVGVLR 100
TVSCGGWVYI TSTDDHDIHD IFMITYIVLT LPWDIMITRY SSPLTSKNKG 150
LTATIFFGTL FPMIYWYIQH SVQQRAGAYS IYAYFEWSLI LLDIAFDAFA 200
YADFKKIDIV LAFNEKPGNT SFFQIRDSSP INYGEEKSSE LQKSGEKKVE 250
KEKPVARSAT GSYFRFDSFF YLLTNIFNGF LFWSNVTSLL CSIWHFPLWY 300
MGISGYEAAI LGYLGPIFLY LPFVSEAFTQ YGVLLGGIIA IGAYIVQMPE 350
LRLISVAVGT SITVATFVQN LRYITNAETS FSFALTWLLG LVASVILKMG 400
FYTNNPTWVI LDERNGGYNK TALVLTVLFG MLSPYVNSIN FEGKRNAQAK 450
SASLIGKLFL AVGFGSLLFG IHQLLTDSST TIYWAWEGYN ESHGPLPWPW 500
GALTCTVMLF ASLSSVKFMG KPLVPCLLLL ISTAVLSARS ITQWPKYIFG 550
GLLYAIAMLW LVPSYFSALG QVQNIWVYVL SFSVYIIFVL AHVWVVAYAF 600
VPMGWVLREK IETVLAFSST FIIIGALTCK NLNIQLVTMG KKFFIYVFFF 650
AVALLSLTAR FVYDIRPTGI PQPYHPDSQL ITAGIWTIHF GLDNDMWASE 700
DRMINLIKDM ELDVVGLLET DTQRITMGNR DLTSKLAHDL NMYADFGPGP 750
NKHTWGCVLL SKFPIVNSTH HLLPSPVGEL APAIHATLQT YNDTLVDVFV 800
FHSGQEEDEE DRRLQSNYMA KLMGNTTRPA ILLSYLVVDP GEGNYNTYVS 850
ETSGMHDIDP SDDDRWCEYI LYRGLRRTGY ARVARGTITD TELQVGKFQV 900
LSEQALVEHS DSMYEYGHMS EPEYEDMKFP DKFLGEGERG HFYHVFDEPR 950
YYL 953
Length:953
Mass (Da):107,883
Last modified:October 24, 2003 - v2
Checksum:i9F56CCD85824E848
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAC42972.1.
BK006937 Genomic DNA. Translation: DAA07494.1.
PIRiS19427.
RefSeqiNP_009943.2. NM_001178730.1.

Genome annotation databases

EnsemblFungiiYCR017C; YCR017C; YCR017C.
GeneIDi850376.
KEGGisce:YCR017C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X59720 Genomic DNA. Translation: CAC42972.1 .
BK006937 Genomic DNA. Translation: DAA07494.1 .
PIRi S19427.
RefSeqi NP_009943.2. NM_001178730.1.

3D structure databases

ProteinModelPortali P25618.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30996. 83 interactions.
MINTi MINT-4478332.
STRINGi 4932.YCR017C.

Protein family/group databases

TCDBi 9.B.131.1.1. the post-gpi attachment protein (p-gap2) family.

Proteomic databases

MaxQBi P25618.
PaxDbi P25618.
PeptideAtlasi P25618.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR017C ; YCR017C ; YCR017C .
GeneIDi 850376.
KEGGi sce:YCR017C.

Organism-specific databases

CYGDi YCR017c.
SGDi S000000610. CWH43.

Phylogenomic databases

eggNOGi NOG309644.
GeneTreei ENSGT00510000048509.
HOGENOMi HOG000196377.
OMAi GNRDTTQ.
OrthoDBi EOG77DJF6.

Enzyme and pathway databases

BioCyci YEAST:G3O-29332-MONOMER.

Miscellaneous databases

NextBioi 965875.
PROi P25618.

Gene expression databases

Genevestigatori P25618.

Family and domain databases

InterProi IPR005135. Endo/exonuclease/phosphatase.
IPR019402. Frag1/DRAM/Sfk1.
IPR027317. PGAP2IP.
[Graphical view ]
PANTHERi PTHR14859:SF0. PTHR14859:SF0. 1 hit.
Pfami PF10277. Frag1. 1 hit.
[Graphical view ]
SUPFAMi SSF56219. SSF56219. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Valles G., Volckaerts G.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 229; 329; 634 AND 873.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Saccharomyces cerevisiae YCRO17c/CWH43 encodes a putative sensor/transporter protein upstream of the BCK2 branch of the PKC1-dependent cell wall integrity pathway."
    Martin-Yken H., Dagkessamanskaia A., De Groot P., Ram A., Klis F., Francois J.
    Yeast 18:827-840(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-57.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid moiety of GPI anchors to ceramides."
    Umemura M., Fujita M., Yoko-O T., Fukamizu A., Jigami Y.
    Mol. Biol. Cell 18:4304-4316(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF GLY-57; HIS-472; ASP-693; HIS-770; HIS-771; GLU-807; ASP-862 AND ARG-882.
  7. "CWH43 is required for the introduction of ceramides into GPI anchors in Saccharomyces cerevisiae."
    Ghugtyal V., Vionnet C., Roubaty C., Conzelmann A.
    Mol. Microbiol. 65:1493-1502(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-713 AND HIS-802.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCWH43_YEAST
AccessioniPrimary (citable) accession number: P25618
Secondary accession number(s): D6VR25, Q8NIM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 24, 2003
Last modified: May 14, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3

Similar proteinsi