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P25618

- CWH43_YEAST

UniProt

P25618 - CWH43_YEAST

Protein

Protein CWH43

Gene

CWH43

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (24 Oct 2003)
      Previous versions | rss
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    Functioni

    Involved in the maintenance of cell wall integrity. Required for the replacement of the diacylglycerol moiety by ceramides during GPI-anchor maturation.3 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei802 – 8021Curated

    GO - Biological processi

    1. cell wall organization Source: UniProtKB
    2. fungal-type cell wall organization Source: SGD
    3. GPI anchor biosynthetic process Source: SGD

    Keywords - Biological processi

    GPI-anchor biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29332-MONOMER.

    Protein family/group databases

    TCDBi9.B.131.1.1. the post-gpi attachment protein (p-gap2) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein CWH43
    Alternative name(s):
    Calcofluor white hypersensitive protein
    Gene namesi
    Name:CWH43
    Ordered Locus Names:YCR017C
    ORF Names:YCR17C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR017c.
    SGDiS000000610. CWH43.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein
    Note: Concentrates to the bud tip of small budded cells and to the neck of dividing cells.

    GO - Cellular componenti

    1. cellular bud neck Source: UniProtKB
    2. cellular bud tip Source: UniProtKB
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. integral component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi57 – 571G → R: Causes destabilization of the protein and induces the release of cell wall proteins in the culture medium. 2 Publications
    Mutagenesisi472 – 4721H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
    Mutagenesisi693 – 6931D → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
    Mutagenesisi713 – 7131D → A: Impairs the introduction of ceramides into the GPI anchor. 1 Publication
    Mutagenesisi770 – 7701H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
    Mutagenesisi771 – 7711H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
    Mutagenesisi802 – 8021H → A: Abrogates the introduction of ceramides into the GPI anchor. 1 Publication
    Mutagenesisi807 – 8071E → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
    Mutagenesisi862 – 8621D → A: Impairs the introduction of ceramides into the GPI anchor. 1 Publication
    Mutagenesisi882 – 8821R → A: Abrogates the introduction of ceramides into the GPI anchor. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 953953Protein CWH43PRO_0000021052Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi490 – 4901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi767 – 7671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi792 – 7921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi825 – 8251N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP25618.
    PaxDbiP25618.
    PeptideAtlasiP25618.

    Expressioni

    Gene expression databases

    GenevestigatoriP25618.

    Interactioni

    Protein-protein interaction databases

    BioGridi30996. 83 interactions.
    MINTiMINT-4478332.
    STRINGi4932.YCR017C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25618.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77CytoplasmicSequence Analysis
    Topological domaini29 – 6436ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini86 – 916CytoplasmicSequence Analysis
    Topological domaini113 – 1175ExtracellularSequence Analysis
    Topological domaini139 – 14911CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini171 – 1755ExtracellularSequence Analysis
    Topological domaini197 – 27882CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini300 – 3078ExtracellularSequence Analysis
    Topological domaini329 – 3302CytoplasmicSequence Analysis
    Topological domaini352 – 3521ExtracellularSequence Analysis
    Topological domaini369 – 38012CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini402 – 42019ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini442 – 4509CytoplasmicSequence Analysis
    Topological domaini472 – 49524ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini517 – 5171CytoplasmicSequence Analysis
    Topological domaini539 – 5479ExtracellularSequence Analysis
    Topological domaini569 – 58517CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini607 – 6137ExtracellularSequence Analysis
    Topological domaini635 – 6428CytoplasmicSequence Analysis
    Topological domaini664 – 953290ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei65 – 8521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei92 – 11221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei118 – 13821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei150 – 17021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei176 – 19621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei279 – 29921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei308 – 32821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei331 – 35121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei353 – 36816HelicalSequence AnalysisAdd
    BLAST
    Transmembranei381 – 40121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei421 – 44121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei451 – 47121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei496 – 51621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei518 – 53821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei548 – 56821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei586 – 60621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei614 – 63421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei643 – 66321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 229229PGAP2-likeAdd
    BLAST
    Regioni230 – 953724PGAP2IP-likeAdd
    BLAST
    Regioni862 – 88221Required for function in lipid remodelingAdd
    BLAST

    Domaini

    The PGAP2-like region interacts with the PGAP2IP-like region.1 Publication

    Sequence similaritiesi

    In the N-terminal section; belongs to the PGAP2 family.Curated
    In the C-terminal section; belongs to the PGAP2IP family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG309644.
    GeneTreeiENSGT00510000048509.
    HOGENOMiHOG000196377.
    OMAiGNRDTTQ.
    OrthoDBiEOG77DJF6.

    Family and domain databases

    InterProiIPR005135. Endo/exonuclease/phosphatase.
    IPR019402. Frag1/DRAM/Sfk1.
    IPR027317. PGAP2IP.
    [Graphical view]
    PANTHERiPTHR14859:SF0. PTHR14859:SF0. 1 hit.
    PfamiPF10277. Frag1. 1 hit.
    [Graphical view]
    SUPFAMiSSF56219. SSF56219. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P25618-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLIINGKIIP IAHTICAFSA FFAALVTGYS LHFHKIVTNA HYTYPDEWFP    50
    SVSATIGDRY PERSIFQILI ALTAFPRFLL LLGHYYLNQS KVCFLVGVLR 100
    TVSCGGWVYI TSTDDHDIHD IFMITYIVLT LPWDIMITRY SSPLTSKNKG 150
    LTATIFFGTL FPMIYWYIQH SVQQRAGAYS IYAYFEWSLI LLDIAFDAFA 200
    YADFKKIDIV LAFNEKPGNT SFFQIRDSSP INYGEEKSSE LQKSGEKKVE 250
    KEKPVARSAT GSYFRFDSFF YLLTNIFNGF LFWSNVTSLL CSIWHFPLWY 300
    MGISGYEAAI LGYLGPIFLY LPFVSEAFTQ YGVLLGGIIA IGAYIVQMPE 350
    LRLISVAVGT SITVATFVQN LRYITNAETS FSFALTWLLG LVASVILKMG 400
    FYTNNPTWVI LDERNGGYNK TALVLTVLFG MLSPYVNSIN FEGKRNAQAK 450
    SASLIGKLFL AVGFGSLLFG IHQLLTDSST TIYWAWEGYN ESHGPLPWPW 500
    GALTCTVMLF ASLSSVKFMG KPLVPCLLLL ISTAVLSARS ITQWPKYIFG 550
    GLLYAIAMLW LVPSYFSALG QVQNIWVYVL SFSVYIIFVL AHVWVVAYAF 600
    VPMGWVLREK IETVLAFSST FIIIGALTCK NLNIQLVTMG KKFFIYVFFF 650
    AVALLSLTAR FVYDIRPTGI PQPYHPDSQL ITAGIWTIHF GLDNDMWASE 700
    DRMINLIKDM ELDVVGLLET DTQRITMGNR DLTSKLAHDL NMYADFGPGP 750
    NKHTWGCVLL SKFPIVNSTH HLLPSPVGEL APAIHATLQT YNDTLVDVFV 800
    FHSGQEEDEE DRRLQSNYMA KLMGNTTRPA ILLSYLVVDP GEGNYNTYVS 850
    ETSGMHDIDP SDDDRWCEYI LYRGLRRTGY ARVARGTITD TELQVGKFQV 900
    LSEQALVEHS DSMYEYGHMS EPEYEDMKFP DKFLGEGERG HFYHVFDEPR 950
    YYL 953
    Length:953
    Mass (Da):107,883
    Last modified:October 24, 2003 - v2
    Checksum:i9F56CCD85824E848
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAC42972.1.
    BK006937 Genomic DNA. Translation: DAA07494.1.
    PIRiS19427.
    RefSeqiNP_009943.2. NM_001178730.1.

    Genome annotation databases

    EnsemblFungiiYCR017C; YCR017C; YCR017C.
    GeneIDi850376.
    KEGGisce:YCR017C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAC42972.1 .
    BK006937 Genomic DNA. Translation: DAA07494.1 .
    PIRi S19427.
    RefSeqi NP_009943.2. NM_001178730.1.

    3D structure databases

    ProteinModelPortali P25618.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30996. 83 interactions.
    MINTi MINT-4478332.
    STRINGi 4932.YCR017C.

    Protein family/group databases

    TCDBi 9.B.131.1.1. the post-gpi attachment protein (p-gap2) family.

    Proteomic databases

    MaxQBi P25618.
    PaxDbi P25618.
    PeptideAtlasi P25618.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR017C ; YCR017C ; YCR017C .
    GeneIDi 850376.
    KEGGi sce:YCR017C.

    Organism-specific databases

    CYGDi YCR017c.
    SGDi S000000610. CWH43.

    Phylogenomic databases

    eggNOGi NOG309644.
    GeneTreei ENSGT00510000048509.
    HOGENOMi HOG000196377.
    OMAi GNRDTTQ.
    OrthoDBi EOG77DJF6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29332-MONOMER.

    Miscellaneous databases

    NextBioi 965875.
    PROi P25618.

    Gene expression databases

    Genevestigatori P25618.

    Family and domain databases

    InterProi IPR005135. Endo/exonuclease/phosphatase.
    IPR019402. Frag1/DRAM/Sfk1.
    IPR027317. PGAP2IP.
    [Graphical view ]
    PANTHERi PTHR14859:SF0. PTHR14859:SF0. 1 hit.
    Pfami PF10277. Frag1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56219. SSF56219. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Valles G., Volckaerts G.
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 229; 329; 634 AND 873.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Saccharomyces cerevisiae YCRO17c/CWH43 encodes a putative sensor/transporter protein upstream of the BCK2 branch of the PKC1-dependent cell wall integrity pathway."
      Martin-Yken H., Dagkessamanskaia A., De Groot P., Ram A., Klis F., Francois J.
      Yeast 18:827-840(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-57.
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    6. "Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid moiety of GPI anchors to ceramides."
      Umemura M., Fujita M., Yoko-O T., Fukamizu A., Jigami Y.
      Mol. Biol. Cell 18:4304-4316(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF GLY-57; HIS-472; ASP-693; HIS-770; HIS-771; GLU-807; ASP-862 AND ARG-882.
    7. "CWH43 is required for the introduction of ceramides into GPI anchors in Saccharomyces cerevisiae."
      Ghugtyal V., Vionnet C., Roubaty C., Conzelmann A.
      Mol. Microbiol. 65:1493-1502(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASP-713 AND HIS-802.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCWH43_YEAST
    AccessioniPrimary (citable) accession number: P25618
    Secondary accession number(s): D6VR25, Q8NIM1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: October 24, 2003
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3