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P25618

- CWH43_YEAST

UniProt

P25618 - CWH43_YEAST

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Protein

Protein CWH43

Gene

CWH43

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the maintenance of cell wall integrity. Required for the replacement of the diacylglycerol moiety by ceramides during GPI-anchor maturation.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei802 – 8021Curated

GO - Biological processi

  1. cell wall organization Source: UniProtKB
  2. fungal-type cell wall organization Source: SGD
  3. GPI anchor biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-29332-MONOMER.

Protein family/group databases

TCDBi9.B.131.1.1. the post-gpi attachment protein (p-gap2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CWH43
Alternative name(s):
Calcofluor white hypersensitive protein
Gene namesi
Name:CWH43
Ordered Locus Names:YCR017C
ORF Names:YCR17C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome III

Organism-specific databases

CYGDiYCR017c.
SGDiS000000610. CWH43.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein
Note: Concentrates to the bud tip of small budded cells and to the neck of dividing cells.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Topological domaini29 – 6436ExtracellularSequence AnalysisAdd
BLAST
Transmembranei65 – 8521HelicalSequence AnalysisAdd
BLAST
Topological domaini86 – 916CytoplasmicSequence Analysis
Transmembranei92 – 11221HelicalSequence AnalysisAdd
BLAST
Topological domaini113 – 1175ExtracellularSequence Analysis
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST
Topological domaini139 – 14911CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence AnalysisAdd
BLAST
Topological domaini171 – 1755ExtracellularSequence Analysis
Transmembranei176 – 19621HelicalSequence AnalysisAdd
BLAST
Topological domaini197 – 27882CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei279 – 29921HelicalSequence AnalysisAdd
BLAST
Topological domaini300 – 3078ExtracellularSequence Analysis
Transmembranei308 – 32821HelicalSequence AnalysisAdd
BLAST
Topological domaini329 – 3302CytoplasmicSequence Analysis
Transmembranei331 – 35121HelicalSequence AnalysisAdd
BLAST
Topological domaini352 – 3521ExtracellularSequence Analysis
Transmembranei353 – 36816HelicalSequence AnalysisAdd
BLAST
Topological domaini369 – 38012CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei381 – 40121HelicalSequence AnalysisAdd
BLAST
Topological domaini402 – 42019ExtracellularSequence AnalysisAdd
BLAST
Transmembranei421 – 44121HelicalSequence AnalysisAdd
BLAST
Topological domaini442 – 4509CytoplasmicSequence Analysis
Transmembranei451 – 47121HelicalSequence AnalysisAdd
BLAST
Topological domaini472 – 49524ExtracellularSequence AnalysisAdd
BLAST
Transmembranei496 – 51621HelicalSequence AnalysisAdd
BLAST
Topological domaini517 – 5171CytoplasmicSequence Analysis
Transmembranei518 – 53821HelicalSequence AnalysisAdd
BLAST
Topological domaini539 – 5479ExtracellularSequence Analysis
Transmembranei548 – 56821HelicalSequence AnalysisAdd
BLAST
Topological domaini569 – 58517CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei586 – 60621HelicalSequence AnalysisAdd
BLAST
Topological domaini607 – 6137ExtracellularSequence Analysis
Transmembranei614 – 63421HelicalSequence AnalysisAdd
BLAST
Topological domaini635 – 6428CytoplasmicSequence Analysis
Transmembranei643 – 66321HelicalSequence AnalysisAdd
BLAST
Topological domaini664 – 953290ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cellular bud neck Source: UniProtKB
  2. cellular bud tip Source: UniProtKB
  3. endoplasmic reticulum Source: UniProtKB-KW
  4. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi57 – 571G → R: Causes destabilization of the protein and induces the release of cell wall proteins in the culture medium. 2 Publications
Mutagenesisi472 – 4721H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi693 – 6931D → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi713 – 7131D → A: Impairs the introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi770 – 7701H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi771 – 7711H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi802 – 8021H → A: Abrogates the introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi807 – 8071E → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi862 – 8621D → A: Impairs the introduction of ceramides into the GPI anchor. 1 Publication
Mutagenesisi882 – 8821R → A: Abrogates the introduction of ceramides into the GPI anchor. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 953953Protein CWH43PRO_0000021052Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi490 – 4901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi767 – 7671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi792 – 7921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi825 – 8251N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP25618.
PaxDbiP25618.
PeptideAtlasiP25618.

Expressioni

Gene expression databases

GenevestigatoriP25618.

Interactioni

Protein-protein interaction databases

BioGridi30996. 84 interactions.
MINTiMINT-4478332.
STRINGi4932.YCR017C.

Structurei

3D structure databases

ProteinModelPortaliP25618.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 229229PGAP2-likeAdd
BLAST
Regioni230 – 953724PGAP2IP-likeAdd
BLAST
Regioni862 – 88221Required for function in lipid remodelingAdd
BLAST

Domaini

The PGAP2-like region interacts with the PGAP2IP-like region.1 Publication

Sequence similaritiesi

In the N-terminal section; belongs to the PGAP2 family.Curated
In the C-terminal section; belongs to the PGAP2IP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG309644.
GeneTreeiENSGT00510000048509.
HOGENOMiHOG000196377.
InParanoidiP25618.
OMAiGNRDTTQ.
OrthoDBiEOG77DJF6.

Family and domain databases

InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR019402. Frag1/DRAM/Sfk1.
IPR027317. PGAP2IP.
[Graphical view]
PANTHERiPTHR14859:SF0. PTHR14859:SF0. 1 hit.
PfamiPF10277. Frag1. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequencei

Sequence statusi: Complete.

P25618-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLIINGKIIP IAHTICAFSA FFAALVTGYS LHFHKIVTNA HYTYPDEWFP
60 70 80 90 100
SVSATIGDRY PERSIFQILI ALTAFPRFLL LLGHYYLNQS KVCFLVGVLR
110 120 130 140 150
TVSCGGWVYI TSTDDHDIHD IFMITYIVLT LPWDIMITRY SSPLTSKNKG
160 170 180 190 200
LTATIFFGTL FPMIYWYIQH SVQQRAGAYS IYAYFEWSLI LLDIAFDAFA
210 220 230 240 250
YADFKKIDIV LAFNEKPGNT SFFQIRDSSP INYGEEKSSE LQKSGEKKVE
260 270 280 290 300
KEKPVARSAT GSYFRFDSFF YLLTNIFNGF LFWSNVTSLL CSIWHFPLWY
310 320 330 340 350
MGISGYEAAI LGYLGPIFLY LPFVSEAFTQ YGVLLGGIIA IGAYIVQMPE
360 370 380 390 400
LRLISVAVGT SITVATFVQN LRYITNAETS FSFALTWLLG LVASVILKMG
410 420 430 440 450
FYTNNPTWVI LDERNGGYNK TALVLTVLFG MLSPYVNSIN FEGKRNAQAK
460 470 480 490 500
SASLIGKLFL AVGFGSLLFG IHQLLTDSST TIYWAWEGYN ESHGPLPWPW
510 520 530 540 550
GALTCTVMLF ASLSSVKFMG KPLVPCLLLL ISTAVLSARS ITQWPKYIFG
560 570 580 590 600
GLLYAIAMLW LVPSYFSALG QVQNIWVYVL SFSVYIIFVL AHVWVVAYAF
610 620 630 640 650
VPMGWVLREK IETVLAFSST FIIIGALTCK NLNIQLVTMG KKFFIYVFFF
660 670 680 690 700
AVALLSLTAR FVYDIRPTGI PQPYHPDSQL ITAGIWTIHF GLDNDMWASE
710 720 730 740 750
DRMINLIKDM ELDVVGLLET DTQRITMGNR DLTSKLAHDL NMYADFGPGP
760 770 780 790 800
NKHTWGCVLL SKFPIVNSTH HLLPSPVGEL APAIHATLQT YNDTLVDVFV
810 820 830 840 850
FHSGQEEDEE DRRLQSNYMA KLMGNTTRPA ILLSYLVVDP GEGNYNTYVS
860 870 880 890 900
ETSGMHDIDP SDDDRWCEYI LYRGLRRTGY ARVARGTITD TELQVGKFQV
910 920 930 940 950
LSEQALVEHS DSMYEYGHMS EPEYEDMKFP DKFLGEGERG HFYHVFDEPR

YYL
Length:953
Mass (Da):107,883
Last modified:October 24, 2003 - v2
Checksum:i9F56CCD85824E848
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42972.1.
BK006937 Genomic DNA. Translation: DAA07494.1.
PIRiS19427.
RefSeqiNP_009943.2. NM_001178730.1.

Genome annotation databases

EnsemblFungiiYCR017C; YCR017C; YCR017C.
GeneIDi850376.
KEGGisce:YCR017C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42972.1 .
BK006937 Genomic DNA. Translation: DAA07494.1 .
PIRi S19427.
RefSeqi NP_009943.2. NM_001178730.1.

3D structure databases

ProteinModelPortali P25618.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 30996. 84 interactions.
MINTi MINT-4478332.
STRINGi 4932.YCR017C.

Protein family/group databases

TCDBi 9.B.131.1.1. the post-gpi attachment protein (p-gap2) family.

Proteomic databases

MaxQBi P25618.
PaxDbi P25618.
PeptideAtlasi P25618.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YCR017C ; YCR017C ; YCR017C .
GeneIDi 850376.
KEGGi sce:YCR017C.

Organism-specific databases

CYGDi YCR017c.
SGDi S000000610. CWH43.

Phylogenomic databases

eggNOGi NOG309644.
GeneTreei ENSGT00510000048509.
HOGENOMi HOG000196377.
InParanoidi P25618.
OMAi GNRDTTQ.
OrthoDBi EOG77DJF6.

Enzyme and pathway databases

BioCyci YEAST:G3O-29332-MONOMER.

Miscellaneous databases

NextBioi 965875.
PROi P25618.

Gene expression databases

Genevestigatori P25618.

Family and domain databases

InterProi IPR005135. Endo/exonuclease/phosphatase.
IPR019402. Frag1/DRAM/Sfk1.
IPR027317. PGAP2IP.
[Graphical view ]
PANTHERi PTHR14859:SF0. PTHR14859:SF0. 1 hit.
Pfami PF10277. Frag1. 1 hit.
[Graphical view ]
SUPFAMi SSF56219. SSF56219. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete DNA sequence of yeast chromosome III."
    Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
    , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
    Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Valles G., Volckaerts G.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 229; 329; 634 AND 873.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Saccharomyces cerevisiae YCRO17c/CWH43 encodes a putative sensor/transporter protein upstream of the BCK2 branch of the PKC1-dependent cell wall integrity pathway."
    Martin-Yken H., Dagkessamanskaia A., De Groot P., Ram A., Klis F., Francois J.
    Yeast 18:827-840(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-57.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid moiety of GPI anchors to ceramides."
    Umemura M., Fujita M., Yoko-O T., Fukamizu A., Jigami Y.
    Mol. Biol. Cell 18:4304-4316(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF GLY-57; HIS-472; ASP-693; HIS-770; HIS-771; GLU-807; ASP-862 AND ARG-882.
  7. "CWH43 is required for the introduction of ceramides into GPI anchors in Saccharomyces cerevisiae."
    Ghugtyal V., Vionnet C., Roubaty C., Conzelmann A.
    Mol. Microbiol. 65:1493-1502(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-713 AND HIS-802.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCWH43_YEAST
AccessioniPrimary (citable) accession number: P25618
Secondary accession number(s): D6VR25, Q8NIM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 24, 2003
Last modified: October 29, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

External Data

Dasty 3