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Protein

Protein CWH43

Gene

CWH43

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maintenance of cell wall integrity. Required for the replacement of the diacylglycerol moiety by ceramides during GPI-anchor maturation.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei802Curated1

GO - Biological processi

  • cell wall organization Source: UniProtKB
  • fungal-type cell wall organization Source: SGD
  • GPI anchor biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-29332-MONOMER.

Protein family/group databases

TCDBi9.B.131.1.1. the post-gpi attachment protein (p-gap2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CWH43
Alternative name(s):
Calcofluor white hypersensitive protein
Gene namesi
Name:CWH43
Ordered Locus Names:YCR017C
ORF Names:YCR17C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR017C.
SGDiS000000610. CWH43.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Topological domaini29 – 64ExtracellularSequence analysisAdd BLAST36
Transmembranei65 – 85HelicalSequence analysisAdd BLAST21
Topological domaini86 – 91CytoplasmicSequence analysis6
Transmembranei92 – 112HelicalSequence analysisAdd BLAST21
Topological domaini113 – 117ExtracellularSequence analysis5
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
Topological domaini139 – 149CytoplasmicSequence analysisAdd BLAST11
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Topological domaini171 – 175ExtracellularSequence analysis5
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 278CytoplasmicSequence analysisAdd BLAST82
Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
Topological domaini300 – 307ExtracellularSequence analysis8
Transmembranei308 – 328HelicalSequence analysisAdd BLAST21
Topological domaini329 – 330CytoplasmicSequence analysis2
Transmembranei331 – 351HelicalSequence analysisAdd BLAST21
Topological domaini352ExtracellularSequence analysis1
Transmembranei353 – 368HelicalSequence analysisAdd BLAST16
Topological domaini369 – 380CytoplasmicSequence analysisAdd BLAST12
Transmembranei381 – 401HelicalSequence analysisAdd BLAST21
Topological domaini402 – 420ExtracellularSequence analysisAdd BLAST19
Transmembranei421 – 441HelicalSequence analysisAdd BLAST21
Topological domaini442 – 450CytoplasmicSequence analysis9
Transmembranei451 – 471HelicalSequence analysisAdd BLAST21
Topological domaini472 – 495ExtracellularSequence analysisAdd BLAST24
Transmembranei496 – 516HelicalSequence analysisAdd BLAST21
Topological domaini517CytoplasmicSequence analysis1
Transmembranei518 – 538HelicalSequence analysisAdd BLAST21
Topological domaini539 – 547ExtracellularSequence analysis9
Transmembranei548 – 568HelicalSequence analysisAdd BLAST21
Topological domaini569 – 585CytoplasmicSequence analysisAdd BLAST17
Transmembranei586 – 606HelicalSequence analysisAdd BLAST21
Topological domaini607 – 613ExtracellularSequence analysis7
Transmembranei614 – 634HelicalSequence analysisAdd BLAST21
Topological domaini635 – 642CytoplasmicSequence analysis8
Transmembranei643 – 663HelicalSequence analysisAdd BLAST21
Topological domaini664 – 953ExtracellularSequence analysisAdd BLAST290

GO - Cellular componenti

  • cellular bud neck Source: UniProtKB
  • cellular bud tip Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57G → R: Causes destabilization of the protein and induces the release of cell wall proteins in the culture medium. 2 Publications1
Mutagenesisi472H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication1
Mutagenesisi693D → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication1
Mutagenesisi713D → A: Impairs the introduction of ceramides into the GPI anchor. 1 Publication1
Mutagenesisi770H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication1
Mutagenesisi771H → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication1
Mutagenesisi802H → A: Abrogates the introduction of ceramides into the GPI anchor. 1 Publication1
Mutagenesisi807E → A: No effect on introduction of ceramides into the GPI anchor. 1 Publication1
Mutagenesisi862D → A: Impairs the introduction of ceramides into the GPI anchor. 1 Publication1
Mutagenesisi882R → A: Abrogates the introduction of ceramides into the GPI anchor. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000210521 – 953Protein CWH43Add BLAST953

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi419N-linked (GlcNAc...)Sequence analysis1
Glycosylationi490N-linked (GlcNAc...)Sequence analysis1
Glycosylationi767N-linked (GlcNAc...)Sequence analysis1
Glycosylationi792N-linked (GlcNAc...)Sequence analysis1
Glycosylationi825N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP25618.
PRIDEiP25618.

PTM databases

iPTMnetiP25618.

Interactioni

Protein-protein interaction databases

BioGridi30996. 84 interactors.
MINTiMINT-4478332.

Structurei

3D structure databases

ProteinModelPortaliP25618.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 229PGAP2-likeAdd BLAST229
Regioni230 – 953PGAP2IP-likeAdd BLAST724
Regioni862 – 882Required for function in lipid remodelingAdd BLAST21

Domaini

The PGAP2-like region interacts with the PGAP2IP-like region.1 Publication

Sequence similaritiesi

In the N-terminal section; belongs to the PGAP2 family.Curated
In the C-terminal section; belongs to the PGAP2IP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000048509.
HOGENOMiHOG000196377.
InParanoidiP25618.
OMAiGNRDTTQ.
OrthoDBiEOG092C05MJ.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR019402. Frag1/DRAM/Sfk1.
IPR027317. PGAP2IP.
[Graphical view]
PANTHERiPTHR14859. PTHR14859. 1 hit.
PfamiPF10277. Frag1. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequencei

Sequence statusi: Complete.

P25618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIINGKIIP IAHTICAFSA FFAALVTGYS LHFHKIVTNA HYTYPDEWFP
60 70 80 90 100
SVSATIGDRY PERSIFQILI ALTAFPRFLL LLGHYYLNQS KVCFLVGVLR
110 120 130 140 150
TVSCGGWVYI TSTDDHDIHD IFMITYIVLT LPWDIMITRY SSPLTSKNKG
160 170 180 190 200
LTATIFFGTL FPMIYWYIQH SVQQRAGAYS IYAYFEWSLI LLDIAFDAFA
210 220 230 240 250
YADFKKIDIV LAFNEKPGNT SFFQIRDSSP INYGEEKSSE LQKSGEKKVE
260 270 280 290 300
KEKPVARSAT GSYFRFDSFF YLLTNIFNGF LFWSNVTSLL CSIWHFPLWY
310 320 330 340 350
MGISGYEAAI LGYLGPIFLY LPFVSEAFTQ YGVLLGGIIA IGAYIVQMPE
360 370 380 390 400
LRLISVAVGT SITVATFVQN LRYITNAETS FSFALTWLLG LVASVILKMG
410 420 430 440 450
FYTNNPTWVI LDERNGGYNK TALVLTVLFG MLSPYVNSIN FEGKRNAQAK
460 470 480 490 500
SASLIGKLFL AVGFGSLLFG IHQLLTDSST TIYWAWEGYN ESHGPLPWPW
510 520 530 540 550
GALTCTVMLF ASLSSVKFMG KPLVPCLLLL ISTAVLSARS ITQWPKYIFG
560 570 580 590 600
GLLYAIAMLW LVPSYFSALG QVQNIWVYVL SFSVYIIFVL AHVWVVAYAF
610 620 630 640 650
VPMGWVLREK IETVLAFSST FIIIGALTCK NLNIQLVTMG KKFFIYVFFF
660 670 680 690 700
AVALLSLTAR FVYDIRPTGI PQPYHPDSQL ITAGIWTIHF GLDNDMWASE
710 720 730 740 750
DRMINLIKDM ELDVVGLLET DTQRITMGNR DLTSKLAHDL NMYADFGPGP
760 770 780 790 800
NKHTWGCVLL SKFPIVNSTH HLLPSPVGEL APAIHATLQT YNDTLVDVFV
810 820 830 840 850
FHSGQEEDEE DRRLQSNYMA KLMGNTTRPA ILLSYLVVDP GEGNYNTYVS
860 870 880 890 900
ETSGMHDIDP SDDDRWCEYI LYRGLRRTGY ARVARGTITD TELQVGKFQV
910 920 930 940 950
LSEQALVEHS DSMYEYGHMS EPEYEDMKFP DKFLGEGERG HFYHVFDEPR

YYL
Length:953
Mass (Da):107,883
Last modified:October 24, 2003 - v2
Checksum:i9F56CCD85824E848
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42972.1.
BK006937 Genomic DNA. Translation: DAA07494.1.
PIRiS19427.
RefSeqiNP_009943.2. NM_001178730.1.

Genome annotation databases

EnsemblFungiiCAC42972; CAC42972; CAC42972.
YCR017C; YCR017C; YCR017C.
GeneIDi850376.
KEGGisce:YCR017C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59720 Genomic DNA. Translation: CAC42972.1.
BK006937 Genomic DNA. Translation: DAA07494.1.
PIRiS19427.
RefSeqiNP_009943.2. NM_001178730.1.

3D structure databases

ProteinModelPortaliP25618.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi30996. 84 interactors.
MINTiMINT-4478332.

Protein family/group databases

TCDBi9.B.131.1.1. the post-gpi attachment protein (p-gap2) family.

PTM databases

iPTMnetiP25618.

Proteomic databases

MaxQBiP25618.
PRIDEiP25618.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAC42972; CAC42972; CAC42972.
YCR017C; YCR017C; YCR017C.
GeneIDi850376.
KEGGisce:YCR017C.

Organism-specific databases

EuPathDBiFungiDB:YCR017C.
SGDiS000000610. CWH43.

Phylogenomic databases

GeneTreeiENSGT00510000048509.
HOGENOMiHOG000196377.
InParanoidiP25618.
OMAiGNRDTTQ.
OrthoDBiEOG092C05MJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-29332-MONOMER.

Miscellaneous databases

PROiP25618.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR019402. Frag1/DRAM/Sfk1.
IPR027317. PGAP2IP.
[Graphical view]
PANTHERiPTHR14859. PTHR14859. 1 hit.
PfamiPF10277. Frag1. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCWH43_YEAST
AccessioniPrimary (citable) accession number: P25618
Secondary accession number(s): D6VR25, Q8NIM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: October 24, 2003
Last modified: November 30, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome III
    Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.