P25613 (ADY2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Accumulation of dyads protein 2

Alternative name(s):
Ammonia transport outward protein 1

Gene names

Name:	ADY2
Synonyms:	ATO1
Ordered Locus Names:	YCR010C
ORF Names:	YCR10C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transporter protein required for ammonia export and acetate uptake and resistance. Necessary for up-regulation and down-regulation of meiotic plaque (MP) component levels in a dependency on external acetate. Has a role in ascus formation. Ref.6 Ref.7 Ref.8 Ref.9 Ref.11
Subcellular location	Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Note: Localizes to large detergent resistant patches of the cell membrane (DRM) enriched in ergosterol and sphingolipids. Ref.9 Ref.10
Induction	During meiosis and by external ammonia. Ref.9 Ref.10
Sequence similarities	Belongs to the GPR1/FUN34/YaaH family.

Ontologies

Keywords
Biological process	Ammonia transport Ion transport Meiosis Transport
Cellular component	Cell membrane Membrane Vacuole
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	meiosis Inferred from electronic annotation. Source: UniProtKB-KW nitrogen utilization Inferred from mutant phenotype Ref.7. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD plasma membrane Inferred from direct assay PubMed 16622836Ref.10. Source: SGD vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetate transmembrane transporter activity Inferred from mutant phenotype Ref.8. Source: SGD ammonium transmembrane transporter activity Inferred from mutant phenotype Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Accumulation of dyads protein 2		PRO_0000135706
Regions
	Topological domain	1 – 89	89	Cytoplasmic Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Topological domain	111 – 120	10	Extracellular Potential
	Transmembrane	121 – 141	21	Helical; Potential
	Topological domain	142 – 151	10	Cytoplasmic Potential
	Transmembrane	152 – 172	21	Helical; Potential
	Topological domain	173 – 185	13	Extracellular Potential
	Transmembrane	186 – 206	21	Helical; Potential
	Topological domain	207 – 208	2	Cytoplasmic Potential
	Transmembrane	209 – 229	21	Helical; Potential
	Topological domain	230 – 240	11	Extracellular Potential
	Transmembrane	241 – 261	21	Helical; Potential
	Topological domain	262 – 283	22	Cytoplasmic Potential
Amino acid modifications
	Modified residue	47	1	Phosphothreonine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P25613 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 36DE421B09D8BB40	FASTA	283	30,726
	10 20 30 40 50 60 MSDKEQTSGN TDLENAPAGY YSSHDNDVNG VAEDERPSHD SLGKIYTGGD NNEYIYIGRQ 70 80 90 100 110 120 KFLKSDLYQA FGGTLNPGLA PAPVHKFANP APLGLSAFAL TTFVLSMFNA RAQGITVPNV 130 140 150 160 170 180 VVGCAMFYGG LVQLIAGIWE IALENTFGGT ALCSYGGFWL SFAAIYIPWF GILEAYEDNE 190 200 210 220 230 240 SDLNNALGFY LLGWAIFTFG LTVCTMKSTV MFFLLFFLLA LTFLLLSIGH FANRLGVTRA 250 260 270 280 GGVLGVVVAF IAWYNAYAGV ATKQNSYVLA RPFPLPSTER VIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of a 10.8 kb segment distal of SUF2 on the right arm of chromosome III from Saccharomyces cerevisiae reveals seven open reading frames including the RVS161, ADP1 and PGK genes." Skala J., Purnelle B., Goffeau A. Yeast 8:409-417(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[5]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-47, MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[6]	"A screen for genes required for meiosis and spore formation based on whole-genome expression." Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E., Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E., Klein F., Knop M., Nasmyth K. Curr. Biol. 11:1001-1009(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Ammonia pulses and metabolic oscillations guide yeast colony development." Palkova Z., Devaux F., Icicova M., Minarikova L., Le Crom S., Jacq C. Mol. Biol. Cell 13:3901-3914(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae." Paiva S., Devaux F., Barbosa S., Jacq C., Casal M. Yeast 21:201-210(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system." Taxis C., Keller P., Kavagiou Z., Jensen L.J., Colombelli J., Bork P., Stelzer E.H.K., Knop M. J. Cell Biol. 171:627-640(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[10]	"Association of putative ammonium exporters Ato with detergent-resistant compartments of plasma membrane during yeast colony development: pH affects Ato1p localisation in patches." Ricicova M., Kucerova H., Vachova L., Palkova Z. Biochim. Biophys. Acta 1768:1170-1178(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INDUCTION.
[11]	"Mutations at different sites in members of the Gpr1/Fun34/YaaH protein family cause hypersensitivity to acetic acid in Saccharomyces cerevisiae as well as in Yarrowia lipolytica." Gentsch M., Kuschel M., Schlegel S., Barth G. FEMS Yeast Res. 7:380-390(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59720 Genomic DNA. Translation: CAA42327.1. BK006937 Genomic DNA. Translation: DAA07488.1.
PIR	S19420.
RefSeq	NP_009936.1. NM_001178723.1.
3D structure databases
ProteinModelPortal	P25613.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7894N.
MINT	MINT-1355103.
STRING	4932.YCR010C.
Protein family/group databases
TCDB	2.A.96.1.4. YaaH family.
Proteomic databases
PaxDb	P25613.
PeptideAtlas	P25613.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCR010C; YCR010C; YCR010C.
GeneID	850368.
KEGG	sce:YCR010C.
Organism-specific databases
CYGD	YCR010c.
SGD	S000000603. ADY2.
Phylogenomic databases
eggNOG	COG1584.
GeneTree	ENSGT00390000006887.
HOGENOM	HOG000224167.
KO	K07034.
OMA	VPNVVVS.
OrthoDB	EOG425932.
Gene expression databases
Genevestigator	P25613.
GermOnline	YCR010C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000791. Grp1_Fun34_YaaH. [Graphical view]
Pfam	PF01184. Grp1_Fun34_YaaH. 1 hit. [Graphical view]
PROSITE	PS01114. GPR1_FUN34_YAAH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	965855.

Entry information

Entry name

ADY2_YEAST

Accession

Primary (citable) accession number: P25613
Secondary accession number(s): D6VR19

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program