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P25613

- ADY2_YEAST

UniProt

P25613 - ADY2_YEAST

Protein

Accumulation of dyads protein 2

Gene

ADY2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Transporter protein required for ammonia export and acetate uptake and resistance. Necessary for up-regulation and down-regulation of meiotic plaque (MP) component levels in a dependency on external acetate. Has a role in ascus formation.5 Publications

    GO - Molecular functioni

    1. acetate transmembrane transporter activity Source: SGD
    2. ammonium transmembrane transporter activity Source: SGD

    GO - Biological processi

    1. acetate transmembrane transport Source: GOC
    2. acetate transport Source: SGD
    3. ammonium transmembrane transport Source: GOC
    4. ammonium transport Source: SGD
    5. meiotic nuclear division Source: UniProtKB-KW
    6. nitrogen utilization Source: SGD
    7. transmembrane transport Source: SGD

    Keywords - Biological processi

    Ammonia transport, Ion transport, Meiosis, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29327-MONOMER.

    Protein family/group databases

    TCDBi2.A.96.1.4. 2.a.96. the acetate uptake transporter (acetr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Accumulation of dyads protein 2
    Alternative name(s):
    Ammonia transport outward protein 1
    Gene namesi
    Name:ADY2
    Synonyms:ATO1
    Ordered Locus Names:YCR010C
    ORF Names:YCR10C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome III

    Organism-specific databases

    CYGDiYCR010c.
    SGDiS000000603. ADY2.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein
    Note: Localizes to large detergent resistant patches of the cell membrane (DRM) enriched in ergosterol and sphingolipids.

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD
    3. vacuolar membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 283283Accumulation of dyads protein 2PRO_0000135706Add
    BLAST

    Proteomic databases

    PaxDbiP25613.
    PeptideAtlasiP25613.

    Expressioni

    Inductioni

    During meiosis and by external ammonia.2 Publications

    Gene expression databases

    GenevestigatoriP25613.

    Interactioni

    Protein-protein interaction databases

    BioGridi30989. 11 interactions.
    DIPiDIP-7894N.
    IntActiP25613. 4 interactions.
    MINTiMINT-1355103.
    STRINGi4932.YCR010C.

    Structurei

    3D structure databases

    ProteinModelPortaliP25613.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8989CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini111 – 12010ExtracellularSequence Analysis
    Topological domaini142 – 15110CytoplasmicSequence Analysis
    Topological domaini173 – 18513ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini207 – 2082CytoplasmicSequence Analysis
    Topological domaini230 – 24011ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini262 – 28322CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei90 – 11021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei121 – 14121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei152 – 17221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei186 – 20621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei209 – 22921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei241 – 26121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1584.
    GeneTreeiENSGT00390000006887.
    HOGENOMiHOG000224167.
    KOiK07034.
    OMAiFFLIGWA.
    OrthoDBiEOG7BKD57.

    Family and domain databases

    InterProiIPR000791. Grp1_Fun34_YaaH.
    [Graphical view]
    PfamiPF01184. Grp1_Fun34_YaaH. 1 hit.
    [Graphical view]
    PROSITEiPS01114. GPR1_FUN34_YAAH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25613-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDKEQTSGN TDLENAPAGY YSSHDNDVNG VAEDERPSHD SLGKIYTGGD    50
    NNEYIYIGRQ KFLKSDLYQA FGGTLNPGLA PAPVHKFANP APLGLSAFAL 100
    TTFVLSMFNA RAQGITVPNV VVGCAMFYGG LVQLIAGIWE IALENTFGGT 150
    ALCSYGGFWL SFAAIYIPWF GILEAYEDNE SDLNNALGFY LLGWAIFTFG 200
    LTVCTMKSTV MFFLLFFLLA LTFLLLSIGH FANRLGVTRA GGVLGVVVAF 250
    IAWYNAYAGV ATKQNSYVLA RPFPLPSTER VIF 283
    Length:283
    Mass (Da):30,726
    Last modified:May 1, 1992 - v1
    Checksum:i36DE421B09D8BB40
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42327.1.
    BK006937 Genomic DNA. Translation: DAA07488.1.
    PIRiS19420.
    RefSeqiNP_009936.1. NM_001178723.1.

    Genome annotation databases

    EnsemblFungiiYCR010C; YCR010C; YCR010C.
    GeneIDi850368.
    KEGGisce:YCR010C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59720 Genomic DNA. Translation: CAA42327.1 .
    BK006937 Genomic DNA. Translation: DAA07488.1 .
    PIRi S19420.
    RefSeqi NP_009936.1. NM_001178723.1.

    3D structure databases

    ProteinModelPortali P25613.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 30989. 11 interactions.
    DIPi DIP-7894N.
    IntActi P25613. 4 interactions.
    MINTi MINT-1355103.
    STRINGi 4932.YCR010C.

    Protein family/group databases

    TCDBi 2.A.96.1.4. 2.a.96. the acetate uptake transporter (acetr) family.

    Proteomic databases

    PaxDbi P25613.
    PeptideAtlasi P25613.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YCR010C ; YCR010C ; YCR010C .
    GeneIDi 850368.
    KEGGi sce:YCR010C.

    Organism-specific databases

    CYGDi YCR010c.
    SGDi S000000603. ADY2.

    Phylogenomic databases

    eggNOGi COG1584.
    GeneTreei ENSGT00390000006887.
    HOGENOMi HOG000224167.
    KOi K07034.
    OMAi FFLIGWA.
    OrthoDBi EOG7BKD57.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29327-MONOMER.

    Miscellaneous databases

    NextBioi 965855.

    Gene expression databases

    Genevestigatori P25613.

    Family and domain databases

    InterProi IPR000791. Grp1_Fun34_YaaH.
    [Graphical view ]
    Pfami PF01184. Grp1_Fun34_YaaH. 1 hit.
    [Graphical view ]
    PROSITEi PS01114. GPR1_FUN34_YAAH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete sequence of a 10.8 kb segment distal of SUF2 on the right arm of chromosome III from Saccharomyces cerevisiae reveals seven open reading frames including the RVS161, ADP1 and PGK genes."
      Skala J., Purnelle B., Goffeau A.
      Yeast 8:409-417(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The complete DNA sequence of yeast chromosome III."
      Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M.
      , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
      Nature 357:38-46(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    5. Cited for: FUNCTION.
    6. "Ammonia pulses and metabolic oscillations guide yeast colony development."
      Palkova Z., Devaux F., Icicova M., Minarikova L., Le Crom S., Jacq C.
      Mol. Biol. Cell 13:3901-3914(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae."
      Paiva S., Devaux F., Barbosa S., Jacq C., Casal M.
      Yeast 21:201-210(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system."
      Taxis C., Keller P., Kavagiou Z., Jensen L.J., Colombelli J., Bork P., Stelzer E.H.K., Knop M.
      J. Cell Biol. 171:627-640(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
    9. "Association of putative ammonium exporters Ato with detergent-resistant compartments of plasma membrane during yeast colony development: pH affects Ato1p localisation in patches."
      Ricicova M., Kucerova H., Vachova L., Palkova Z.
      Biochim. Biophys. Acta 1768:1170-1178(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INDUCTION.
    10. "Mutations at different sites in members of the Gpr1/Fun34/YaaH protein family cause hypersensitivity to acetic acid in Saccharomyces cerevisiae as well as in Yarrowia lipolytica."
      Gentsch M., Kuschel M., Schlegel S., Barth G.
      FEMS Yeast Res. 7:380-390(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
      Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
      Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.

    Entry informationi

    Entry nameiADY2_YEAST
    AccessioniPrimary (citable) accession number: P25613
    Secondary accession number(s): D6VR19
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome III
      Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

    External Data

    Dasty 3