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P25613 (ADY2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Accumulation of dyads protein 2
Alternative name(s):
Ammonia transport outward protein 1
Gene names
Name:ADY2
Synonyms:ATO1
Ordered Locus Names:YCR010C
ORF Names:YCR10C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transporter protein required for ammonia export and acetate uptake and resistance. Necessary for up-regulation and down-regulation of meiotic plaque (MP) component levels in a dependency on external acetate. Has a role in ascus formation. Ref.6 Ref.7 Ref.8 Ref.9 Ref.11

Subcellular location

Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Note: Localizes to large detergent resistant patches of the cell membrane (DRM) enriched in ergosterol and sphingolipids. Ref.9 Ref.10

Induction

During meiosis and by external ammonia. Ref.9 Ref.10

Sequence similarities

Belongs to the GPR1/FUN34/yaaH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 283283Accumulation of dyads protein 2
PRO_0000135706

Regions

Topological domain1 – 8989Cytoplasmic Potential
Transmembrane90 – 11021Helical; Potential
Topological domain111 – 12010Extracellular Potential
Transmembrane121 – 14121Helical; Potential
Topological domain142 – 15110Cytoplasmic Potential
Transmembrane152 – 17221Helical; Potential
Topological domain173 – 18513Extracellular Potential
Transmembrane186 – 20621Helical; Potential
Topological domain207 – 2082Cytoplasmic Potential
Transmembrane209 – 22921Helical; Potential
Topological domain230 – 24011Extracellular Potential
Transmembrane241 – 26121Helical; Potential
Topological domain262 – 28322Cytoplasmic Potential

Amino acid modifications

Modified residue471Phosphothreonine Ref.5

Sequences

Sequence LengthMass (Da)Tools
P25613 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 36DE421B09D8BB40

FASTA28330,726
        10         20         30         40         50         60 
MSDKEQTSGN TDLENAPAGY YSSHDNDVNG VAEDERPSHD SLGKIYTGGD NNEYIYIGRQ 

        70         80         90        100        110        120 
KFLKSDLYQA FGGTLNPGLA PAPVHKFANP APLGLSAFAL TTFVLSMFNA RAQGITVPNV 

       130        140        150        160        170        180 
VVGCAMFYGG LVQLIAGIWE IALENTFGGT ALCSYGGFWL SFAAIYIPWF GILEAYEDNE 

       190        200        210        220        230        240 
SDLNNALGFY LLGWAIFTFG LTVCTMKSTV MFFLLFFLLA LTFLLLSIGH FANRLGVTRA 

       250        260        270        280 
GGVLGVVVAF IAWYNAYAGV ATKQNSYVLA RPFPLPSTER VIF

« Hide

References

« Hide 'large scale' references
[1]"The complete sequence of a 10.8 kb segment distal of SUF2 on the right arm of chromosome III from Saccharomyces cerevisiae reveals seven open reading frames including the RVS161, ADP1 and PGK genes."
Skala J., Purnelle B., Goffeau A.
Yeast 8:409-417(1992) [PubMed: 1626432] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete DNA sequence of yeast chromosome III."
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. expand/collapse author list , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.
Nature 357:38-46(1992) [PubMed: 1574125] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[5]"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-47, MASS SPECTROMETRY.
Strain: ATCC 76625 / YPH499.
[6]"A screen for genes required for meiosis and spore formation based on whole-genome expression."
Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E., Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E., Klein F., Knop M., Nasmyth K.
Curr. Biol. 11:1001-1009(2001) [PubMed: 11470404] [Abstract]
Cited for: FUNCTION.
[7]"Ammonia pulses and metabolic oscillations guide yeast colony development."
Palkova Z., Devaux F., Icicova M., Minarikova L., Le Crom S., Jacq C.
Mol. Biol. Cell 13:3901-3914(2002) [PubMed: 12429834] [Abstract]
Cited for: FUNCTION.
[8]"Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae."
Paiva S., Devaux F., Barbosa S., Jacq C., Casal M.
Yeast 21:201-210(2004) [PubMed: 14968426] [Abstract]
Cited for: FUNCTION.
[9]"Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system."
Taxis C., Keller P., Kavagiou Z., Jensen L.J., Colombelli J., Bork P., Stelzer E.H.K., Knop M.
J. Cell Biol. 171:627-640(2005) [PubMed: 16286509] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[10]"Association of putative ammonium exporters Ato with detergent-resistant compartments of plasma membrane during yeast colony development: pH affects Ato1p localisation in patches."
Ricicova M., Kucerova H., Vachova L., Palkova Z.
Biochim. Biophys. Acta 1768:1170-1178(2007) [PubMed: 17395151] [Abstract]
Cited for: SUBCELLULAR LOCATION, INDUCTION.
[11]"Mutations at different sites in members of the Gpr1/Fun34/YaaH protein family cause hypersensitivity to acetic acid in Saccharomyces cerevisiae as well as in Yarrowia lipolytica."
Gentsch M., Kuschel M., Schlegel S., Barth G.
FEMS Yeast Res. 7:380-390(2007) [PubMed: 17233767] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X59720 Genomic DNA. Translation: CAA42327.1.
BK006937 Genomic DNA. Translation: DAA07488.1.
PIRS19420.
RefSeqNP_009936.1. NM_001178723.1.

3D structure databases

ProteinModelPortalP25613.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-7894N.
MINTMINT-1355103.
STRINGP25613.

Protein family/group databases

TCDB2.A.96.1.4. YaaH family.

Proteomic databases

PeptideAtlasP25613.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYCR010C; YCR010C; YCR010C.
GeneID850368.
KEGGsce:YCR010C.
NMPDRfig|4932.3.peg.659.

Organism-specific databases

CYGDYCR010c.
SGDS000000603. ADY2.

Phylogenomic databases

eggNOGfuNOG05598.
GeneTreeEFGT00050000003883.
HOGENOMHBG736287.
OMAVPNVVVS.
OrthoDBEOG425932.

Gene expression databases

ArrayExpressP25613.
GenevestigatorP25613.
GermOnlineYCR010C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000791. Grp1_Fun34_YaaH.
[Graphical view]
KOK07034.
PfamPF01184. Grp1_Fun34_YaaH. 1 hit.
[Graphical view]
PROSITEPS01114. GPR1_FUN34_YAAH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio965855.

Entry information

Entry nameADY2_YEAST
AccessionPrimary (citable) accession number: P25613
Secondary accession number(s): D6VR19
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: December 14, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome III

Yeast (Saccharomyces cerevisiae) chromosome III: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents