Reviewed, UniProtKB/Swiss-Prot P25613 (ADY2_YEAST)

Names and origin

Protein names

Recommended name:
    Accumulation of dyads protein 2
Alternative name(s):
    Ammonia transport outward protein 1

Gene names

Name:	ADY2
Synonyms:	ATO1
Ordered Locus Names:	YCR010C
ORF Names:	YCR10C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transporter protein required for ammonia export and acetate uptake and resistance. Necessary for up-regulation and down-regulation of meiotic plaque (MP) component levels in a dependency on external acetate. Has a role in ascus formation. Ref.5 Ref.6 Ref.7 Ref.8 Ref.10
Subcellular location	Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Note: Localizes to large detergent resistent patches of the cell membrane (DRM) enriched in ergosterol and sphingolipids. Ref.8 Ref.9
Induction	During meiosis and by external ammonia. Ref.8 Ref.9
Sequence similarities	Belongs to the GPR1/FUN34/yaaH family.

Ontologies

Keywords
Biological process	Ammonia transport Ion transport Meiosis Transport
Cellular component	Cell membrane Membrane Vacuole
Domain	Transmembrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	acetate transport Ref.7 Inferred from mutant phenotype. Source: SGD ammonium transport Ref.6 Inferred from expression pattern. Source: SGD meiosis Inferred from electronic annotation. Source: UniProtKB-KW nitrogen utilization Ref.6 Inferred from mutant phenotype. Source: SGD transmembrane transport Ref.7 Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay. Source: SGD plasma membrane Ref.9 Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD vacuole Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetate transmembrane transporter activity Ref.7 Inferred from mutant phenotype. Source: SGD ammonium transmembrane transporter activity Ref.6 Inferred from expression pattern. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Accumulation of dyads protein 2		PRO_0000135706
Regions
	Topological domain	1 – 89	89	Cytoplasmic Potential
	Transmembrane	90 – 110	21	Potential
	Topological domain	111 – 120	10	Extracellular Potential
	Transmembrane	121 – 141	21	Potential
	Topological domain	142 – 151	10	Cytoplasmic Potential
	Transmembrane	152 – 172	21	Potential
	Topological domain	173 – 185	13	Extracellular Potential
	Transmembrane	186 – 206	21	Potential
	Topological domain	207 – 208	2	Cytoplasmic Potential
	Transmembrane	209 – 229	21	Potential
	Topological domain	230 – 240	11	Extracellular Potential
	Transmembrane	241 – 261	21	Potential
	Topological domain	262 – 283	22	Cytoplasmic Potential
Amino acid modifications
	Modified residue	47	1	Phosphothreonine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P25613-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 36DE421B09D8BB40	FASTA	283	30,726
	10 20 30 40 50 60 MSDKEQTSGN TDLENAPAGY YSSHDNDVNG VAEDERPSHD SLGKIYTGGD NNEYIYIGRQ 70 80 90 100 110 120 KFLKSDLYQA FGGTLNPGLA PAPVHKFANP APLGLSAFAL TTFVLSMFNA RAQGITVPNV 130 140 150 160 170 180 VVGCAMFYGG LVQLIAGIWE IALENTFGGT ALCSYGGFWL SFAAIYIPWF GILEAYEDNE 190 200 210 220 230 240 SDLNNALGFY LLGWAIFTFG LTVCTMKSTV MFFLLFFLLA LTFLLLSIGH FANRLGVTRA 250 260 270 280 GGVLGVVVAF IAWYNAYAGV ATKQNSYVLA RPFPLPSTER VIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequence of a 10.8 kb segment distal of SUF2 on the right arm of chromosome III from Saccharomyces cerevisiae reveals seven open reading frames including the RVS161, ADP1 and PGK genes." Skala J., Purnelle B., Goffeau A. Yeast 8:409-417(1992) [PubMed: 1626432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[4]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-47, MASS SPECTROMETRY.
[5]	"A screen for genes required for meiosis and spore formation based on whole-genome expression." Rabitsch K.P., Toth A., Galova M., Schleiffer A., Schaffner G., Aigner E., Rupp C., Penkner A.M., Moreno-Borchart A.C., Primig M., Esposito R.E., Klein F., Knop M., Nasmyth K. Curr. Biol. 11:1001-1009(2001) [PubMed: 11470404] [Abstract] Cited for: FUNCTION.
[6]	"Ammonia pulses and metabolic oscillations guide yeast colony development." Palkova Z., Devaux F., Icicova M., Minarikova L., Le Crom S., Jacq C. Mol. Biol. Cell 13:3901-3914(2002) [PubMed: 12429834] [Abstract] Cited for: FUNCTION.
[7]	"Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae." Paiva S., Devaux F., Barbosa S., Jacq C., Casal M. Yeast 21:201-210(2004) [PubMed: 14968426] [Abstract] Cited for: FUNCTION.
[8]	"Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system." Taxis C., Keller P., Kavagiou Z., Jensen L.J., Colombelli J., Bork P., Stelzer E.H.K., Knop M. J. Cell Biol. 171:627-640(2005) [PubMed: 16286509] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[9]	"Association of putative ammonium exporters Ato with detergent-resistant compartments of plasma membrane during yeast colony development: pH affects Ato1p localisation in patches." Ricicova M., Kucerova H., Vachova L., Palkova Z. Biochim. Biophys. Acta 1768:1170-1178(2007) [PubMed: 17395151] [Abstract] Cited for: SUBCELLULAR LOCATION, INDUCTION.
[10]	"Mutations at different sites in members of the Gpr1/Fun34/YaaH protein family cause hypersensitivity to acetic acid in Saccharomyces cerevisiae as well as in Yarrowia lipolytica." Gentsch M., Kuschel M., Schlegel S., Barth G. FEMS Yeast Res. 7:380-390(2007) [PubMed: 17233767] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59720 Genomic DNA. Translation: CAA42327.1.
PIR	S19420.
RefSeq	NP_009936.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7894N.
STRING	P25613.
Protein family/group databases
TCDB	2.A.96.1.4. YaaH family.
Proteomic databases
PeptideAtlas	P25613.
Genome annotation databases
Ensembl	YCR010C; YCR010C; YCR010C; Saccharomyces cerevisiae. [Genome view]
GeneID	850368.
KEGG	sce:YCR010C.
NMPDR	fig|4932.3.peg.659.
Organism-specific databases
CYGD	YCR010c.
SGD	S000000603. ADY2.
Phylogenomic databases
HOGENOM	HBG736287.
OMA	VPNVVVS.
OrthoDB	EOG9WDFTX.
Gene expression databases
ArrayExpress	P25613.
Genevestigator	P25613.
GermOnline	YCR010C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000791. Grp1_Fun34_YaaH. [Graphical view]
Pfam	PF01184. Grp1_Fun34_YaaH. 1 hit. [Graphical view]
PROSITE	PS01114. GPR1_FUN34_YAAH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	965855.

Entry information

Entry name

ADY2_YEAST

Accession

Primary (citable) accession number: P25613

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	December 15, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)